9 results about "Spore wall" patented technology

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

This invention discloses an online detection system and method for the cell wall breakage rate of Ganoderma lucidum spore powder, belonging to the field of online detection technology. This online detection system and method for Ganoderma lucidum spore powder cell wall breakage rate completes the initial screening through circumferential grayscale feature analysis of parallel and perpendicular polarized signals, quickly achieving the separation of broken spores in the initial screening. Through radial grayscale gradient analysis, the number of jump rings corresponding to the number of intact spore wall layers is determined, achieving refined classification of unbroken, partially broken, and broken spores in the initial screening, solving the problem that existing technologies cannot identify the partially broken state. A secondary circumferential grayscale feature verification and breakpoint quantification analysis are performed on the unbroken spores in the initial screening to remove false unbroken spores with localized spore wall damage. Using the characteristic spectrum of the original effective components inside the spore as a benchmark, the release behavior of the effective components is anchored through homology matching of internal and external spectra and radial intensity distribution verification, distinguishing between effective cell wall breakage and ineffective breakage, thus improving the accuracy of Ganoderma lucidum spore powder cell wall breakage rate detection.

The invention discloses a ganoderma spore wall-breaking, extracting and freeze-drying integrated method which comprises the following steps: firstly, mixing ganoderma spore powder with a specific wall-breaking medium which consists of beta-cyclodextrin, sodium alginate, vitamin C sodium salt and the like, and forming uniform suspension at 45-55 DEG C; secondly, carrying out stepped variable-temperature wall breaking treatment, and physically destroying a spore wall structure through multiple cycles of freezing at-25 DEG C to-20 DEG C and quick unfreezing at 60-70 DEG C; then, the pH is adjusted, a compound enzyme preparation is added for synergistic enzymolysis, and intracellular active substances are efficiently released; then, performing targeted enrichment on components such as polysaccharide by adopting a gradient ethanol precipitation method; finally, a final product is obtained through program temperature control vacuum freeze drying. The method effectively solves the problems of tedious process, large loss of active ingredients and high energy consumption of the traditional method. The method has the advantages of mild conditions and no need of complex equipment, and the obtained product has the advantages of high wall breaking rate, complete reservation of active components and good solubility, and is suitable for industrial production of high-quality ganoderma spore products.

The present application relates to the technical field of traditional Chinese medicine processing, and specifically discloses a broken wall powder processing technology for ganoderma spore powder, which comprises the following steps: S1: selecting ganoderma spore powder raw materials for cleaning, filtering impurities and unqualified ganoderma spore powder after washing, separating qualified ganoderma spore powder, and performing low-temperature vacuum drying to obtain dried ganoderma spore powder; S2: conveying the dried ganoderma spore powder to a dry ice freezing cabin in a sealed manner, keeping the temperature constant for 30-45 minutes in a low-temperature environment, so that the spore wall is changed from toughness to brittleness, creating a structural basis for subsequent physical broken wall; by first cooling the ganoderma spore powder, the spore wall is changed from toughness to brittleness, and then is taken away by high-speed airflow and impacted with the inner wall and grinding balls of the ball mill to break the wall, and then is continuously impacted by the ball mill to break the wall, so that, compared with directly using the ball mill, the broken wall effect is better, the broken wall success rate is further improved, and the use temperature of the ball mill can be reduced to maintain the component activity of the ganoderma spore powder.

The invention relates to the technical field of ganoderma lucidum spore oil extraction, and discloses an efficient ganoderma lucidum spore oil extraction device and process, the process comprises pretreatment, supercritical extraction, separation and purification and post-treatment.The pretreatment device comprising a microwave wall breaking unit is additionally arranged, efficient cell wall breaking treatment is carried out on ganoderma lucidum spore raw materials, and the extraction efficiency is improved; the compact structure of a spore wall is destroyed, so that internal grease is easier to release, and the extraction efficiency of a subsequent extraction process and the yield of a target product are improved; by adopting an extraction system which takes supercritical carbon dioxide as a main body and cooperates with an entrainer, the specific high diffusivity and strong dissolving capacity of supercritical fluid are utilized, and the solubilizing effect of the entrainer on polar components is combined, so that fat-soluble and partial polar active components in the ganoderma lucidum spores are efficiently and selectively extracted; the extraction rate and the comprehensive quality of an extraction product are improved.

The invention relates to the field of food processing, and discloses wall-removed wall-broken ganoderma lucidum spore powder and a preparation method thereof.The preparation method comprises the following steps that A, ganoderma lucidum spore powder is taken as a raw material and subjected to pretreatment, pre-humidification and low-temperature shearing wall breaking, and wall-broken ganoderma lucidum spore powder is prepared; b, performing settling separation on spore wall shells and spore kernels in the sporoderm-broken ganoderma lucidum spore powder, and recovering settling liquid; and C, drying the wet material subjected to settling separation, and carrying out microscopic examination layering to obtain the wall-removed wall-broken ganoderma lucidum spore powder. The preparation process is simple and efficient, the ganoderma lucidum spore wall shells are separated by adopting a pure physical means, and the ganoderma lucidum spore wall shells in the obtained wall-removed wall-broken ganoderma lucidum spore powder are obviously less than those in wall-broken ganoderma lucidum spore powder prepared by adopting a general wall-breaking means; compared with the wall-broken ganoderma lucidum spore powder which is not prepared by the preparation method, the wall-removed wall-broken ganoderma lucidum spore powder has higher functional component content, higher absorption speed and higher absorption rate, and has great commercial value.

The invention provides an LAMP (loop-mediated isothermal amplification) primer group for detecting antheraea pernyi, a detection method and application, and belongs to the technical field of pathogenic microorganism detection. The LAMP primer group is designed according to a plurality of regions of a target gene spore wall protein NpSWP13 DNA sequence, so that only a target sequence can be effectively amplified. According to the LAMP detection method, the nosema antheraeae can be effectively distinguished from other tussah diseases, the nosema antheraeae with extremely low content can be detected, the LAMP detection method has extremely high sensitivity, only a constant-temperature water bath kettle or other constant-temperature equipment is needed during detection, an expensive PCR instrument is not needed, the operation is simple, a result can be obtained by reacting for 60 minutes at the constant temperature of 65 DEG C, and the method is suitable for large-scale popularization and application. The detection result is visual and can be directly judged by naked eyes. In addition, the invention is of great significance to centralized quarantine of antheraea pernyi pebrine and germplasm resource protection in the antheraea pernyi industry.

The invention relates to the technical field of ganoderma lucidum spore powder processing, in particular to a ganoderma lucidum spore powder processing method for pharmaceutical excipients, which comprises the following steps: obtaining an original ganoderma lucidum spore powder raw material, and placing the original ganoderma lucidum spore powder raw material into a spiral conveying sterilization cabin; the method comprises the following steps: acquiring biological characteristic data of a raw material based on a preset spore activation target, and selecting a first biological enzyme preparation from a pre-established enzyme preparation library based on the data; the rotating speed of a screw rod of the spiral conveying sterilization cabin is controlled within the range of 5-25 rpm, and the temperature of a jacket is controlled within the range of 45-65 DEG C, so that the materials in the cabin reach and maintain a first preset process environment; in a first preset process environment, passing through a sectional type injection system integrated in a spiral conveying sterilization cabin; according to the method, the spore wall is mildly deconstructed by using the first bio-enzyme preparation, and the natural germination process of the spore wall is simulated, so that the damage of high temperature, high pressure and strong shearing force to active components is avoided, and the natural nutritional conformation and physiological activity of the ganoderma lucidum spores are furthest reserved.

The utility model relates to the technical field of spore wall breaking, in particular to a ganoderma spore wall breaking device which comprises a box body, a first motor and a wall breaking assembly, supporting columns are arranged at the lower end of the box body, a feeding opening is formed in the upper end of the box body, a first discharging opening is formed in one side of the box body, a second discharging opening is formed in the lower end of the box body, and a wall breaking assembly is arranged on one side of the box body; a first motor is arranged on one side of the box body, a rotating shaft is arranged at the output end of the first motor, a cam is arranged on the outer side of the rotating shaft, a fixing block is arranged on the inner side of the box body, a spring is arranged at the upper end of the fixing block, a sieve plate is arranged on one side of the spring, and a sliding rod is arranged at the lower end of the sieve plate. And the ganoderma lucidum spore powder can smoothly pass through the sieve plate, so that the problems that most sieve plates do not have a vibration function and are low in filtering efficiency, and the ganoderma lucidum spore powder after wall breaking can be accumulated on the filtering sieve plates, so that blockage is caused, the discharging efficiency of the device is reduced, and the production efficiency is reduced are solved.