63 results about "Spore wall" patented technology

The invention discloses an extraction method and application for a ganoderma lucidum spore active composition. The extraction method comprises the following steps: (1) performing softening processing, namely, immersing ganoderma lucidum spore in a mixed enzyme solution containing cellulase with the mass concentration of 0.2-0.5% and pectase with the mass concentration of 0.2-0.5%, so as to soften ganoderma lucidum spore wall; (2) performing low-temperature vacuum drying; and (3) performing low-temperature wall breaking, namely, performing mechanical crushing wall breaking on ganoderma lucidum spore at a low-temperature environment. The method combines a double-enzyme solution and a low-temperature mechanical process for softening ganoderma lucidum spore cell wall, and helps to effectively reduce difficulty for crushing ganoderma lucidum spore cell wall and shorten the time. The extracted ganoderma lucidum spore active composition is free of toxic and side effects on human skin fibroblast, is applicable to anti-ageing cosmetic products, such as facial masks, face cream, emulsion and other cosmetic, and effectively reaches the effects of removing acnes, eliminating pockmark, removing wrinkles, removing fine wrinkles and the like.

The invention discloses a preparation method of a sporopollen microcapsule. The method comprises the following steps: taking unprocessed and complete pollen or spore, immersing the pollen or spore in one or more of alcohol, an alcohol solution, an alkali solution, water, acetone, an acetone solution, chloroform and an acid solution, filtering, cleaning, and drying to obtain the sporopollen microcapsule. The method allows the complete pollen or spore wall to be extracted and to be used for the microcapsule, can remove biomasses in the pollen or spore to make a capsule core substance absorbed through the nanometer channels of the microcapsule in order to realize an encapsulating effect, and also removes active substance having negative influences in the pollen or spore.

The invention discloses a method for cultivating selenium-enriched ganoderma lucidum. The method is characterized by comprising the following steps of: drilling holes on basswood for planting the ganoderma lucidum; respectively planting organic selenium yeast and ganoderma lucidum strains into the holes; plugging wooden plugs into the holes; planting the basswood into wet soil; ensuring certain temperature and humidity; and enabling ganoderma lucidum spore powder planted to be mature to contain the organic selenium yeast with certain amount. Due to use of a ganoderma lucidum spore wall-breaking technology, organic selenium with certain amount is absorbed to a human body after a person takes ganoderma lucidum spores and further medicinal value and economic value of ganoderma lucidum are improved. The content of the organic selenium in the ganoderma lucidum spore powder can be adjusted according to the planted amount of the organic selenium yeast.

The invention provides an enterocytozoon hepatopenaei spore wall protein as well as preparation and application of a polyclonal antibody thereof, and belongs to the technical field of animal quarantine. The enterocytozoon hepatopenaei spore wall protein (EHP00_430) is positioned on the surfaces of enterocytozoon hepatopenaei spores in the infection period, has high abundance, plays an important role in the process that enterocytozoon hepatopenaei infects prawns, and is an important target for detection, prevention and control of enterocytozoon hepatopenaei. The enterocytozoon hepatopenaei spore wall protein provided by the invention is used for preparing a polyclonal antibody for resisting the EHP00_430 protein; and the polyclonal antibody is high in specificity, can be used for accuratelydetecting enterocytozoon hepatopenaei in the infection period, and can be applied to detection of enterocytozoon hepatopenaei pathogens.

The invention discloses a physical and chemical wall dissolving method for ganoderma lucidum spore powder. The method comprises the following steps: adding a treating agent to ganoderma lucidum spore powder; reacting under ultrasonic and freezing treatment, and carrying out ultrasonic treatment again; adding an ethanol solution, reacting at room temperature, carrying out freezing reaction at -10 DEG C; carrying out ultrasonic treatment and centrifugally separating out a solid; and adding an ascorbic acid and a protecting agent liquid, stirring evenly, and drying to obtain a product. By virtue of physical acceleration, a few of chemical substances are adopted to react with the wall of the ganoderma lucidum spore powder; and the water dissolving rate of the ganoderma lucidum spore powder can be up to 99.8%, so that complementary advantages of a physical method and a chemical method are achieved; the wall of the ganoderma lucidum spore powder is dissolved into a mesh structure; dissolution of effective components in the ganoderma lucidum spore powder is facilitated; through wall-dissolving treatment and hole sealing of a protective agent, the spore wall is transformed into hydrophilia from hydrophobicity; formation of a suspension liquid of the ganoderma lucidum spore powder is facilitated; absorption of a human body is promoted; due to hole sealing protection, the loss of the effective components of the ganoderma lucidum spore powder in the storage process can be reduced, and therefore, the physical and chemical wall dissolving method is suitable for industrialized production.

The invention discloses ganoderma spore efficient wall breaking and grinding equipment which comprises a ganoderma spore wall breaking machine, conveying belt equipment, broken wall removing equipmentand a receiving basin. The receiving basin is located below a discharge outlet of the ganoderma spore wall breaking machine, the receiving basin is located on the surface of the conveying belt equipment, the broken wall removing equipment is fixed to one end of the conveying belt equipment, the conveying belt equipment penetrates through the interior of the broken wall removing equipment, the broken wall removing equipment is composed of an equipment shell, a fixing plate, an external connection water pipe, a driving device, a lifting part, an opening part and a sealing part, the bottom of the equipment shell is fixed to the outer side of the conveying belt equipment, the two sides of the equipment shell are provided with feed inlets, and the sealing part is fixed to the outer wall, abovethe feed inlets, of the equipment shell. Impurities and broken walls are removed from wall-broken ganoderma spores in the receiving basin through the broken wall removing equipment, the efficiency ishigher compared with traditional manual removing, the labor intensity of staff is relieved, and the wall breaking and grinding efficiency of ganoderma spores is improved.

The ginkgo leaf and olive leaf health granule is prepared with ginkgo leaf and olive leaf as basic material and spore wall-breaking glossy ganoderma powder or ginseng powder as supplementary material. Ginkgo leaf and olive leaf collected in spring, summer and autumn is processed through heating to soft, kneading, squeezing, rinsing to eliminate bitter and astringency, fumigating, stoving, drying and crushing to prepare the health granule. Taking the ginkgo leaf and olive leaf health granule regularly results in the health functions of preventing cardiac vascular diseases, strengthening physique, lowering serum cholesterol, etc.

The invention discloses chitinase capable of degrading spore walls of myxosporean and encoding genes thereof. The chitinase is a protein with either of the following amino acid residue sequence: 1) SEQ ID No. 2 the amino acid residue sequence in a sequence table; and 2) sequence of a protein which has the related function of the chitinase and is derived from SEQ ID No. 2 by replacing and/or deleting and/or adding one or more amino acid residues in the amino acid residue sequence. Experiments show that the chitinase has the activity for degrading chitin, and can destroy the spore walls of the myxosporean. Therefore, the chitinase and the encoding genes thereof can be used for preventing and controlling myxosporidiosis, particularly fish myxosporidiosis.

The invention discloses a ganoderma lucidum spore wall breaking machine. The ganoderma lucidum spore wall breaking machine comprises a case, a feeding hopper, two wall-breaking rollers, a leakage-proof cover, mounting rods, first brushes, a discharging pipe, a screening barrel, a motor, second brushes, fine powder holes, a fine powder box, a coarse material port and a coarse material box. The feeding hopper is arranged at the top of the case, the lower end of the feeding hopper is communicated with the interior of the case, the two wall-breaking rollers in relative rotation are arranged in thecase and under the lower end of the feeding hopper, and the leakage-proof cover which is capable of covering the two wall-breaking rollers is arranged at the lower end of the feeding hopper. The mounting rods are horizontally and fixedly arranged on two opposite side walls, and each first brush is detachably arranged at one end, facing the periphery of the corresponding wall-breaking roller, of the corresponding mounting rod. Compared with the prior art, the ganoderma lucidum spore wall breaking machine has advantages that high efficiency and quickness in wall breaking and screening of ganoderma lucidum spores can be achieved, efficiency and effects in wall breaking and screening of ganoderma lucidum spores are improved, waste of the ganoderma lucidum spores is effectively prevented, andstructural simplicity and convenience in use are achieved.

The invention discloses a ganoderma spore wall breaking method. According to the method, ganoderma spore powder and fullerene powder are mixed and are pretreated, so that the cluster particles in the fullerene powder are crushed into micrometer and/or nanometer particles to enter the shell walls and the inside of the ganoderma spores; then, an external energy source mode is used for radiating the fullerene so that the fullerene generates phase change and generates volume expansion; the spore outer walls are expanded open; the wall breaking of the ganoderma spores is realized. The invention also discloses a preparation method of wall breaking ganoderma spore powder and the wall breaking ganoderma spore powder prepared by the method. The method does not need high temperature and high pressure; the active ingredient content and the activity of the ganoderma spores are maintained to the maximum degree; the tumor inhibition rate is high; the prepared wall breaking ganoderma spore powder has the obvious curative effect on the liver injury due to chemotherapy. The invention also discloses a preparation method of ganoderma spore oil and the ganoderma spore oil prepared by the method. The ganoderma spore oil is obtained through being extracted from the wall breaking ganoderma spore powder obtained through preparation and the extraction rate is high.

The invention discloses a rapid determination method for haematococcus pluvialis spore wall-broken rate. The method includes the steps of: A. conducting acetone extraction and determining astaxanthin absorbance of a known sample; B. grinding the known sample and performing homogenizing to make the wall-broken rate reach 100%, then conducting acetone extraction, and determining astaxanthin absorbance of the 100% wall-broken sample; and C. conducting acetone extraction and determining astaxanthin absorbance (light absorption value A1) of the known sample, grinding the known sample and performing homogenizing to make the wall-broken rate reach 100%, then conducting acetone extraction, and determining the ratio of astaxanthin absorbance (light absorption value A2) of the 100% wall-broken sample, i.e. a spore wall-broken rate. The method provided by the invention only includes three steps, has the characteristics of simple operation, convenient calculation (calculation of the actual content of astaxanthin is unnecessary, and only through division of the absorbance A1 and A2 of the two samples can a corresponding proportional result be obtained), high efficiency and rapidity (the whole process is about 15-20min), thus being very suitable for real-time monitoring and quality control of a haematococcus pluvialis product production process.

The invention discloses a spore physical wall breaking device and method based on laser light energy, and belongs to the technical field of spore wall breaking. The spore physical wall breaking devicecomprises a mixing barrel, a pipeline pump, a light energy receiver and a laser device; the mixing barrel, the pipeline pump and the light energy receiver are sequentially connected through pipelines, and a spore feeding hopper and a pure water entering pipe are arranged on the mixing barrel; and the pipeline pump is used for conveying a mixed solution in the mixing barrel to the light energy receiver; and the laser device is used for carrying out laser irradiation on the mixed solution in the light energy receiver. The spore physical wall breaking method comprises the following steps that 1,pure water and spores are added into the mixing barrel to obtain the mixed solution; 2, the mixed solution is conveyed into the light energy receiver; 3, laser irradiation is carried out on the sidepart of the light energy receiver by the laser device; and 4, the spores in the mixed solution are subjected to irradiation wall breaking through the light energy receiver and then are conveyed into afinished product barrel. According to the spore physical wall breaking device and method, laser light energy is utilized to carry out non-oxidation and no-additive physical wall breaking on drug-derived plant spores and pollen spores, and the wall breaking rate is high.

The invention discloses application of rabbit encephalitozoon cuniculi spore wall protein SWP1 to preparation of a reagent for diagnosing or detecting rabbit encephalitozoon cuniculi infection. A specific primer is designed according to the gene sequence of the rabbit encephalitozoon cuniculi spore wall protein SWP1, published by a database of NCBI (National Center of Biotechnology Information), and is amplified in a fox kidney sample infected with encephalitozoon cuniculi to obtain the SWP1nucleotide sequence shown as SEQ ID NO.2. The fused-expressed SWP1-GST is used for the detection of the sample, the result shows that the fused-expressed SWP1-GST protein has good specificity on the detection of fox serum infected with rabbit encephalitozoo cuniculi, and has no cross reaction with positive serum infected with toxoplasma gondii, neospora caninum and cryptosporidium. In addition, the research result shows that the sensitivity of the SWP1 protein, which is taken as a detection antigen, is obviously higher than that of the PTP2 (Protein-tyrosine-phosphatase 2) protein as a detection antigen. Therefore, the method for diagnosing or detecting the rabbit encephalitozoonosis cuniculi is high in sensitivity, and the diagnosis and prevention of the rabbit encephalitozoonosis cuniculi are further promoted.

The invention discloses a ganoderma spore wall breaking method. The method comprises crushing, oscillating, enzymolysis, ultrasonic treatment, drying, superfine grinding, freeze drying and ultrasonictreatment processes. The method has the advantages that the enzymolysis, ultrasonic treatment, superfine grinding and freeze drying processes have huge effect on wall breaking, and the oscillating process, within which wall breaking enzyme is added, further has huge effect on the preliminary decomposition of cell wall, so that the later processes exert the biggest effect, the wall breaking efficiency can be improved only through combination of all the steps, the wall breaking rate reaches 99.8 percent, and meanwhile loss of effective components is reduced.

The invention relates to a method for extracting mycotic spore RNA by a mechanical disruption method. The method includes steps of taking mycotic spore liquid, adding glass bead to perform mechanical disruption treatment, and then performing RNA extraction. In the invention, a mechanical method is applied to break a mycotic spore wall, and RNA extraction and purification are carried out by combining with a column purification method after breaking; then reverse transcription is performed by using a kit, and the extracting effect is detected through a real-time fluorescent quantitation PCR and gel electrophoresis; accordingly, a technical system for accurately extracting the mycotic spore RNA is set up. The optimized method for extracting mycotic spore RNA has the advantages of being high in extracting efficiency, convenient and easy to operate, free from toxicity and harm; more importantly, the extracted RNA quality is good, and the RNA can meet the past sequencing requirement of monoplast; the method provides a good reference value for the monospore sequencing in future.

A ganoderma spore wall-breaking method is characterized in that a ganoderma spore is soaked in a soak solution, a coating film covers the outer surface of the ganoderma spore, and the ganoderma sporeis placed in a free-drying room to be subjected to freeze-drying treatment. The coating film covers the outer surface of the ganoderma spore and is bonded to the outer wall of the ganoderma spore, theganoderma spore is placed in the free-drying room, a temperature difference is generated in the freeze-drying room within a short period of time, and the ganoderma spore is wall-broken through fracture of the coating film. According to the ganoderma spore wall breaking method, two materials with different thermal expansion coefficients are utilized to perform wall-breaking on the ganoderma spore,so that the wall-breaking efficiency is high, and effective active ingredients of the ganoderma spore can be reserved.

The invention relates to a preparation method of broken-wall ganoderma lucidum spore powder. The preparation method is characterized by comprising the following steps: screening, collecting powder, and cutting the collected ganoderma lucidum spore powder by using a high-pressure purified air cutting and crushing machine technology at the ultralow temperature of -60 DEG C; removing broken ganoderma lucidum spore walls without containing a bioactive substance by using a low-temperature layered-purifying method; and finally performing anti-oxidation treatment to obtain the broken-wall ganoderma lucidum spore powder. The preparation method has the advantages that original ecological active effective ingredients of the ganoderma lucidum spore powder are maximally stored or not damaged by virtue of low-temperature and high-pressure air cutting, and hard ganoderma lucidum spore cell walls are very easily broken simply, rapidly and effectively.

The invention relates to a technology to soften Ganoderma lucidum spore cover. It adopts wood culture medium to cultivate the Ganoderma lucidum spore, screening to 200-300 items after rough separating, purification, and antisepsis. The feature is that it adopts biotechnology, under the catalysis of enzyme and the temperature between 40-50 degree centigrade, the cover of Ganoderma lucidum would be softened but not broken, and the oiliness of spore would not exuded. It would be kept for 2-4 years in normal temperature. Moreover, the spore cover after softening would be absorbed by human. The invention has advanced technology, simple process and great social benefit and economic benefit.

The invention discloses a method for preparing wall-broken ganoderma lucidum spore powder capsules. First trichoderma viride is cultivated, obtained metabolites are utilized to process collected ganoderma lucidum spore powder, diluted hydrochloric acid is added and then intermittent oscillation processing is performed, so that the spore wall is loosened, the treated spore powder undergoes washing, drying, mechanical crushing and ultraviolet sterilization, and the wall-broken ganoderma lucidum spore powder capsules are obtained. Compared with the case that the wall-broken rate byconventional physical and mechanical crushing is low and it is difficult to achieve the satisfactory wall-broken effects, the method is simple to operate, the mechanical wall breaking is adopted after treatment, so that the wall-broken rate of obtained ganoderma lucidum spores is over 99% and even reaches 100%, and accordingly nutritional ingredients in the ganoderma lucidum spore powder can be released better to be directly absorbed by the human intestines and stomach.

The invention discloses an extraction method of ganoderma lucidum spore powder polysaccharide. The method comprises the following steps: pretreatment; wall breaking treatment; ultrasonic extraction; concentration; and freeze-drying and the like. According to the ganoderma spore wall breaking method, enzymolysis and microwave wall breaking are adopted, the wall breaking rate of ganoderma spores is effectively increased and reaches up to 99%, and effective components in the raw materials can be effectively reserved; and through ultrasonic extraction and vacuum freeze drying, the biological activity of the ganoderma lucidum spore powder polysaccharide is effectively reserved, the influence of physical and chemical factors such as solvents, temperature and pressure is avoided, the ganoderma lucidum spore powder polysaccharide which is fragrant and sweet in cane sugar, high in purity and free of organic solvent residues is finally obtained, and the extraction rate of the ganoderma lucidum spore powder polysaccharide is high and reaches up to 99%.