Wall-removed wall-broken ganoderma lucidum spore powder and preparation method thereof

Abstract

The invention relates to the field of food processing, and discloses wall-removed wall-broken ganoderma lucidum spore powder and a preparation method thereof.The preparation method comprises the following steps that A, ganoderma lucidum spore powder is taken as a raw material and subjected to pretreatment, pre-humidification and low-temperature shearing wall breaking, and wall-broken ganoderma lucidum spore powder is prepared; b, performing settling separation on spore wall shells and spore kernels in the sporoderm-broken ganoderma lucidum spore powder, and recovering settling liquid; and C, drying the wet material subjected to settling separation, and carrying out microscopic examination layering to obtain the wall-removed wall-broken ganoderma lucidum spore powder. The preparation process is simple and efficient, the ganoderma lucidum spore wall shells are separated by adopting a pure physical means, and the ganoderma lucidum spore wall shells in the obtained wall-removed wall-broken ganoderma lucidum spore powder are obviously less than those in wall-broken ganoderma lucidum spore powder prepared by adopting a general wall-breaking means; compared with the wall-broken ganoderma lucidum spore powder which is not prepared by the preparation method, the wall-removed wall-broken ganoderma lucidum spore powder has higher functional component content, higher absorption speed and higher absorption rate, and has great commercial value.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of food processing, in particular to a preparation method of a de-walled broken wall ganoderma spore powder. BACKGROUND

[0002] Ganoderma (scientific name: Ganoderma Lucidum), also known as Reishaw, Xiancao, is a perennial saprophytic fungus of Basidiomycota Polyporaceae. Its fruiting body is mostly umbrella-shaped, with a lacquered surface and a color ranging from red-brown to purple-black. The underside of the cap has a porous tracheal layer to release its spores. Ganoderma prefers warm and humid environments, with an optimal growth temperature of 25-30°C. It is commonly found around the base of broad-leaved tree stumps or around felled logs. It is distributed in East China, South China, and Southwest China, with many species, including Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, and Ganoderma lucidum. Since ancient times, Ganoderma lucidum has been known as a valuable medicinal material in China. According to the "Shennong Bencao Jing", Ganoderma lucidum has a bitter taste and is used to treat chest congestion, improve heart function, and enhance wisdom and intelligence. Long-term consumption can lead to weight loss, youthfulness, and longevity.

[0003] Ganoderma spore powder is the dried mature spore of Ganoderma lucidum (Leyss. ex Fr.) Karst. Ganoderma spores, as the "seeds" of Ganoderma lucidum, contain a large amount of polysaccharides, polypeptides, triterpenes, and Ganoderma spore oil. According to modern research and clinical cases, broken wall Ganoderma spore powder has good regulating and improving effects on human immune cells, nervous system, and cardiovascular system.

[0004] Ganoderma spores have a typical fungal cell structure. The Ganoderma spore kernel, which is the center of genetic material and nutrition, is protected by a double-layer spore wall. The main component of this double-layer wall is chitin, which is hard and accounts for about 50%-70% of the entire Ganoderma spore. Chitin, also known as chitosan, is a polysaccharide widely found in the exoskeleton of arthropods and the cell wall of fungi. It has important applications in medicine, food, agriculture, and other fields. However, chitin is a polysaccharide composed of N-acetylglucosamine, with a stable molecular structure. Humans lack the enzymes or bacteria to break down its glycosidic bonds, making it difficult to be directly digested and absorbed by the digestive tract. Furthermore, the Ganoderma spore wall hinders direct contact between the Ganoderma spore kernel and the digestive juice, resulting in extremely low overall absorption rate when consuming unbroken Ganoderma spore powder. Therefore, most commercially available Ganoderma spore powder products use a breaking wall technology to improve their utilization rate in the human body.

[0005] The current mature methods for breaking the wall of Ganoderma lucidum spore powder include chemical method, biological method and physical method. The chemical method uses strong acid or strong base to chemically destroy the wall of Ganoderma lucidum spore, which has the characteristics of simple operation, high efficiency, uniform degree of wall breaking and high wall breaking rate. However, the chemical residues after wall breaking make it not a mainstream method. The biological method mainly uses biological enzymatic technology to decompose and destroy the wall of Ganoderma lucidum spore, which is safer than chemical method, but still has problems such as long breaking period and low breaking rate. The mainstream method for breaking the wall in the market is physical method, which has advantages such as mature production equipment, high breaking efficiency, and the ability to monitor the breaking rate at any time. However, since it only uses physical means to destroy the structure of the wall of Ganoderma lucidum spore, although it can solve the problem of hindering the absorption of nutritional components of Ganoderma lucidum spore by human body, a large amount of chitin remains after wall breaking, which is mixed with the Ganoderma lucidum spore kernel as the main nutritional part, reducing the overall utilization rate of broken Ganoderma lucidum spore powder.

[0006] In recent years, with the vigorous development of the field of food processing, the wall removal technology of broken Ganoderma lucidum spore powder has developed somewhat. However, the current industry's methods for removing the wall of broken Ganoderma lucidum spore powder are mostly a combination of multiple breaking methods or through water extraction or alcohol extraction of broken Ganoderma lucidum spore powder to obtain Ganoderma lucidum spore powder extract and claim to have achieved wall removal. Such methods are difficult to operate, the product is unstable, or incompatible with the current legal and regulatory requirements for commercial broken Ganoderma lucidum spore powder, which is "low-temperature physical breaking", making the problem of wall removal technology of broken Ganoderma lucidum spore powder still not fundamentally solved.

[0007] Chinese patent CN111418822A discloses a method for preparing wall-removed Ganoderma lucidum spore powder. The preparation process is as follows: after the Ganoderma lucidum spore powder raw material is decontaminated and dried, the activated and cultured 5h-24h Kluyveromyces lactis seed liquid and the activated and cultured 5h-24h Lactobacillus reuteri seed liquid are prepared, the Ganoderma lucidum spore powder is mixed with water to prepare a liquid fermentation medium with a concentration of 5g / L-20g / L and sterilized and cooled, then the Kluyveromyces lactis seed liquid is inoculated into the liquid fermentation medium and cultured for 10h-60h, then placed in an alternating magnetic field environment for further culture for 1h-10h, sterilized and cooled, and then the Lactobacillus reuteri seed liquid is inoculated, cultured for 10h-60h, and the obtained fermentation liquid is dried to obtain wall-removed Ganoderma lucidum spore powder. This preparation method is complex and time-consuming, involves microbial fermentation, and has risks such as incomplete sterilization and microbial metabolite contamination. At the same time, the wall-removed Ganoderma lucidum spore powder prepared by this method does not meet the current relevant product commercialization regulations, and has no commercial value.

[0008] Chinese patent CN107115304A discloses a method for preparing Ganoderma lucidum spore powder tablets with cell wall broken spores. The preparation process involves mixing 13-20 parts of water extract of broken-cell Ganoderma lucidum spore powder, 3-5 parts of sorbitol, and 0.8-1.2 parts of povidone K30 for 10-30 minutes. The resulting mixture is then granulated, sized, and dried to obtain granules. These granules are then compressed into tablets to obtain Ganoderma lucidum spore powder tablets with cell wall broken spores. This preparation method directly treats the water extract of broken-cell Ganoderma lucidum spore powder as Ganoderma lucidum spore powder with cell wall broken spores, confusing the two concepts. It uses raw materials that are not suitable for food as ingredients in the formulation, making it impossible to commercialize and thus lacking commercial value. Summary of the Invention

[0009] The purpose of this invention is to address the shortcomings of existing processes for preparing broken-cell wall Ganoderma lucidum spore powder and decelled-cell wall Ganoderma lucidum spore powder, by providing a safe, reliable, effective, and commercially viable decelled-cell wall broken-cell wall Ganoderma lucidum spore powder and its preparation method. This decelled-cell wall broken-cell wall Ganoderma lucidum spore powder has higher triterpenoid content, higher polysaccharide content, higher absorption rate, faster absorption speed, is easily absorbed, has high bioavailability, and is more suitable for the elderly or those with weak digestive systems. The preparation method of this decelled-cell wall broken-cell wall Ganoderma lucidum spore powder is safe, efficient, and has high resource utilization, making it suitable for large-scale industrial production. This technological disclosure provides a feasible approach for the further commercialization of broken-cell wall Ganoderma lucidum spore powder.

[0010] Based on the physical structure of Ganoderma lucidum spores and the sedimentation characteristics of microparticles in fluids, this invention pre-treats Ganoderma lucidum spore powder by repeatedly and rapidly cooling and heating it before cell wall breaking. While preserving the structure of both, it separates the physical connection between the Ganoderma lucidum spore shell and the Ganoderma lucidum spore kernel. Then, the pre-treated Ganoderma lucidum spore powder is pre-humidified, and the formula of the chilled water in the Ganoderma lucidum spore powder cell wall breaking machine (based on the principle of double roller differential shearing cell wall breaking) is improved to enhance the cell wall breaking effect during low-temperature shearing cell wall breaking and further widen the particle size difference between Ganoderma lucidum spore shell fragments and Ganoderma lucidum spore kernels. In the subsequent sedimentation separation process, the difference in sedimentation velocity caused by the difference in particle size between the two is used to achieve the stratification of Ganoderma lucidum spore shell fragments and Ganoderma lucidum spore kernels. After drying and microscopic examination to confirm the boundary layer depth, the Ganoderma lucidum spore shell fragment layer is separated to obtain cell wall broken Ganoderma lucidum spore powder. The prepared cell wall-removed Ganoderma lucidum spore powder has a significantly reduced amount of residual cell wall shell compared to ordinary cell wall-removed spore powder, and its biological activity is significantly improved, resulting in a higher utilization rate by the human body.

[0011] The method for preparing cell wall-broken Ganoderma lucidum spore powder provided by the present invention includes the following steps: (a) Pretreatment: By rapidly lowering and raising the temperature of Ganoderma lucidum spore powder, the tissue connection between the Ganoderma lucidum spore wall shell and the Ganoderma lucidum spore kernel is destroyed; (b) Pre-humidification: Spray the Ganoderma lucidum spore powder that is about to undergo the cell wall breaking process with a pre-humidification solution and stir evenly; (c) Low-temperature shearing cell disruption: The pre-moistened material is subjected to low-temperature cell disruption using a Ganoderma lucidum spore powder cell disruption machine (based on the principle of double-roller differential shearing cell disruption) to obtain cell-wall disrupted Ganoderma lucidum spore powder; (d) Sedimentation and recovery of sedimentation liquid: The broken-cell wall Ganoderma lucidum spore powder obtained in step c is mixed evenly with a small amount of sedimentation liquid to prepare a broken-cell wall Ganoderma lucidum spore powder solution. This solution is then slowly poured into a sedimentation tank containing the sedimentation liquid to separate the Ganoderma lucidum spore walls and spore kernels. After the sedimentation process is complete, food-grade filter paper is used to cover the bottom of the sedimentation tank to recover the sedimentation liquid and obtain the stratified moist broken-cell wall Ganoderma lucidum spore powder. (e) Drying: The moistened Ganoderma lucidum spore powder that has been separated into layers obtained in step d is dried to obtain the separated Ganoderma lucidum spore powder. (f) Microscopic examination of the layered structure: A conical sampling pit is dug on the layered broken Ganoderma lucidum spore powder obtained in step e. Samples are taken and examined under a microscope to confirm the boundary height between the Ganoderma lucidum spore wall shell layer and the broken Ganoderma lucidum spore powder. The upper layer of Ganoderma lucidum spore wall shell layer is separated by hand shovel to obtain broken Ganoderma lucidum spore powder.

[0012] Furthermore, in the preparation method of the cell wall-breaking Ganoderma lucidum spore powder provided by the present invention, in step (a), the tissue connection between the Ganoderma lucidum spore wall shell and the Ganoderma lucidum spore kernel can be destroyed by cyclic rapid heating and cooling changes 1 to 3 times; rapid cooling is achieved by liquid nitrogen isolation cooling; and rapid heating is achieved by hot water isolation heating at 40℃ to 80℃.

[0013] Furthermore, in the method for preparing cell wall-breaking Ganoderma lucidum spore powder provided by the present invention, in step (b), the pre-humidification solution is purified water; the moisture content of the pre-humidified material ranges from 8% to 15%.

[0014] Furthermore, in the method for preparing cell wall-breaking Ganoderma lucidum spore powder provided by the present invention, in step (c), the cooling water in the chiller unit of the Ganoderma lucidum spore powder cell wall-breaking machine is a 10%-20% concentration of saline solution; the cooling water temperature is -5℃ to -15℃; and under working conditions, the surface temperature range of the double roller components of the Ganoderma lucidum spore powder cell wall-breaking machine is -2℃ to -10℃.

[0015] Furthermore, in the method for preparing cell wall-broken Ganoderma lucidum spore powder provided by the present invention, in step (d), the settling liquid is water or 5%~75% edible alcohol; the material-liquid ratio is 1:5~10.

[0016] Furthermore, in the method for preparing cell wall-broken Ganoderma lucidum spore powder provided by the present invention, in step (e), the drying temperature is 35℃~70℃.

[0017] Furthermore, in the preparation method of the cell wall-removed and broken Ganoderma lucidum spore powder provided by the present invention, in step (f), the microscopic examination method is as follows: 0.15g of sample is taken and diluted to 100mL with purified water. After ultrasonic vibration until the spores are fully dispersed, a drop of the middle layer liquid is drawn with a pipette and placed on a hemocytometer, covered with a coverslip. After the sample is injected, it is allowed to stand for about 30 seconds. Then, the hemocytometer is placed under an 800x optical microscope for observation and counting. Ten small squares are randomly sampled and the number of Ganoderma lucidum spore wall fragments in each small square is recorded. The criterion for determining whether it meets the requirements for cell wall-removed and broken Ganoderma lucidum spore powder is: the average number of Ganoderma lucidum spore wall fragments per small square does not exceed 5.

[0018] The cell wall-breaking Ganoderma lucidum spore powder provided by this invention can be used as a raw material for health food or traditional Chinese medicine decoction pieces. It can be prepared into various dosage forms without the addition of human-acceptable food additives or pharmaceutical excipients, according to the corresponding preparation methods.

[0019] The dosage forms include powders, granules, tablets, granules, powders, oral liquids, film-coated tablets, enteric-coated tablets, hard capsules, soft capsules, lozenges, pills, ointments, boluses, drop pills, disintegrants, orally disintegrating agents, microcapsules, microemulsions, and solid dispersions.

[0020] Compared with existing technologies, this invention has the following beneficial effects: The residual amount of the cell wall in the de-celled Ganoderma lucidum spore powder of this invention is significantly lower than that in ordinary de-celled Ganoderma lucidum spore powder, making it easier to digest and absorb. It has a higher content of triterpenes and polysaccharides, is easily absorbed, has high human utilization rate, higher absorption rate, and faster absorption speed, making it more suitable for the elderly or those with weak gastrointestinal function. It also has health benefits such as anti-tumor, hypoglycemic, hypolipidemic, cholesterol reduction, anti-fatigue, antioxidant, anti-aging, tumor inhibition, improved sleep, improved allergic constitution, reduced inflammatory symptoms, prevention of arteriosclerosis, liver protection, and accelerated blood circulation. The preparation method of the de-celled Ganoderma lucidum spore powder provided by this invention overcomes the technical problems of low cell wall breaking rate, residual microbial metabolites, and incomplete inactivation of fermentation bacteria caused by enzymatic cell wall removal in existing cell wall removal technologies, as well as the inactivation of active ingredients and food safety risks of reaction products during high-temperature extraction caused by cell wall removal. The cell wall breaking process of the Ganoderma lucidum spore powder of this invention adopts a cell wall breaking machine based on the principle of dual-roller differential shearing. It can monitor and precisely control the cell wall breaking progress in real time, ensuring that the cell wall breaking rate meets the standard before subsequent processing. Moreover, the entire cell wall breaking process is carried out in a low-temperature and slow-speed state, which does not cause problems such as heavy metal contamination due to metal wear or material inactivation and denaturation at high temperatures, and is less likely to cause food safety risks. At the same time, this invention adopts low-temperature physical cell wall breaking technology, and the entire preparation process uses technologies or additives that meet the current regulations of relevant food or traditional Chinese medicine decoction pieces, which has high commercial value. Attached Figure Description

[0021] Figure 1 The process flow diagram for preparing cell wall-broken Ganoderma lucidum spore powder.

[0022] Figure 2 This is a photomicrograph of commercially available broken-cell wall Ganoderma lucidum spore powder.

[0023] Figure 3 This is a micrograph of the cell wall-broken Ganoderma lucidum spore powder obtained by the present invention.

[0024] Figure 4 Comparison of residual mass of cell-wall-removed Ganoderma lucidum spore powder and ordinary cell-wall-removed Ganoderma lucidum spore powder in artificial intestinal fluid after 72 hours. Detailed Implementation

[0025] The present invention can be better understood from the following embodiments. However, the specific feed-liquid ratios, process conditions, and results described in the embodiments are for illustrative purposes only and should not, and will not, limit the invention as described in detail in the claims. Example 1

[0026] The preparation method of cell wall-removed and broken Ganoderma lucidum spore powder is attached. Figure 1 As shown, please follow these steps: (1) Take 1000g of cleaned and de-shriveled Ganoderma lucidum spore powder and place it in a stainless steel tray. Prepare a stainless steel deep tray containing liquid nitrogen and a stainless steel deep tray containing 70℃ hot water. Place the stainless steel tray in the stainless steel deep tray containing liquid nitrogen. After the Ganoderma lucidum spore powder is uniformly cooled to -100℃ by monitoring with an infrared thermometer, quickly transfer the stainless steel tray to the stainless steel deep tray containing 70℃ hot water. When the temperature of the Ganoderma lucidum spore powder no longer rises, transfer the stainless steel tray to the stainless steel deep tray containing liquid nitrogen again and add 70℃ hot water to the other stainless steel deep tray. Repeat the above process twice. (2) Spray 60g of purified water evenly onto 1000g of pretreated Ganoderma lucidum spore powder raw material and stir evenly; (3) Replace the chilled water in the Ganoderma lucidum spore powder cell wall breaking machine based on the principle of double roller differential shearing with 15% salt water, and set the cooling water temperature to -10℃. When the double roller parts are cooled to below -8℃, put in the pre-moistened Ganoderma lucidum spore powder and repeatedly break the cell wall until the cell wall breaking rate is higher than 99%, and obtain 980 grams of broken Ganoderma lucidum spore powder. (4) Prepare a 20L stainless steel settling tank containing 4000g of 20% edible alcohol settling liquid. After the cell wall of the broken Ganoderma lucidum spore powder is broken, it is sieved through a 30-mesh sieve and then mixed with 2000g of 20% edible alcohol until uniform. Then slowly pour it into the settling tank and let it stand for 1 hour to allow the Ganoderma lucidum spore shells and Ganoderma lucidum spore kernels to settle and separate into layers. Cut a piece of food-grade filter paper with the same size as the diameter of the settling tank and slowly place it horizontally on the surface of the settling liquid. After it sinks naturally and covers the bottom material, use a siphon tube to recover the excess settling liquid and obtain 2400g of wet broken Ganoderma lucidum spore powder that has been separated into layers. (5) Place the sedimentation tank in a hot air circulating oven and set the temperature to 45°C for drying until the moisture content is less than 6%. Remove the filter paper on the surface to obtain 940 grams of dried layered broken Ganoderma lucidum spore powder. (6) A cone-shaped sampling pit with a diameter of 5 cm and a cone apex angle of 90 degrees was dug on the layered broken Ganoderma lucidum spore powder. For every 0.25 cm increase in depth, 0.15 g of sample was taken for microscopic examination. It was confirmed that the boundary height between the Ganoderma lucidum spore wall shell layer and the broken Ganoderma lucidum spore powder was 1.75 cm from the top surface. The upper layer of Ganoderma lucidum spore wall shell layer was separated by hand shovel to obtain 690 g of broken Ganoderma lucidum spore powder. Example 2

[0027] Detection of effective ingredient content between cell wall-removed and ordinary cell wall-removed Ganoderma lucidum spore powder The cell wall-removed Ganoderma lucidum spore powder produced in Example 1 and the ordinary cell wall-removed Ganoderma lucidum spore powder produced by conventional cell wall-removal process were sampled and tested multiple times for polysaccharides and total triterpenes. The results showed that the polysaccharide and total triterpenes content of the cell wall-removed Ganoderma lucidum spore powder was significantly higher than that of the ordinary cell wall-removed Ganoderma lucidum spore powder (P<0.05), with an average increase of nearly 6 times. The results are shown in Table 1.

[0028] Table 1. Results of Detection of Effective Component Content in Ganoderma lucidum Spore Powder with Cell Wall Removal and Ordinary Cell Wall Removal Sample Polysaccharide content (g / 100g) Total triterpene content (g / 100g) De-walled broken Ganoderma lucidum spore powder 7.540±0.119 9.367±0.105 Common broken Ganoderma lucidum spore powder 1.308±0.075 1.608±0.108 Example 3

[0029] Comparative experiment on the dissolution rate of polysaccharide components in artificial intestinal fluid between cell wall-removed and ordinary cell wall-removed Ganoderma lucidum spore powders within 2 hours. One gram of each of the cell-wall-removed Ganoderma lucidum spore powder produced in Example 1 and the ordinary cell-wall-removed Ganoderma lucidum spore powder produced by conventional cell-wall-removal process was weighed and poured into 100 ml of sterile artificial intestinal fluid (Phygene brand) and stirred evenly. The mixture was then stirred again every 10 minutes to prevent spore powder sedimentation. After the samples had been in the artificial intestinal fluid for 2 hours, the suspension samples were filtered using medium-speed filter paper. The filter residue was dried until the moisture content was below 6%. The remaining polysaccharide content of both samples after the simulated digestion process was measured, and the average absorption rate of both samples over 2 hours was calculated. The results showed that the average polysaccharide absorption rate of the cell-wall-removed Ganoderma lucidum spore powder was approximately 3.1 times that of the ordinary cell-wall-removed Ganoderma lucidum spore powder over 2 hours. The results are shown in Table 2.

[0030] Table 2. Comparison of polysaccharide dissolution rates between cell wall-removed and regular cell wall-removed Ganoderma lucidum spore powder in artificial intestinal fluid within 2 hours. Sample Polysaccharide content before digestion (g / 100g) Polysaccharide content after 2 hours of digestion (g / 100g) Average polysaccharide absorption rate (g / 100g*h) De-walled broken Ganoderma lucidum spore powder 7.54 4.982 1.279 Common broken Ganoderma lucidum spore powder 1.308 0.486 0.411 Example 4

[0031] Comparison of residual mass of cell-wall-removed Ganoderma lucidum spore powder and ordinary cell-wall-removed Ganoderma lucidum spore powder in artificial intestinal fluid after 72 hours Twelve portions (3.000g each) of the cell-wall-removed Ganoderma lucidum spore powder produced in Example 1 and the ordinary cell-wall-removed Ganoderma lucidum spore powder produced using conventional cell-wall-removing processes were accurately weighed and poured into 100ml of sterile artificial intestinal fluid (Phygene brand). The mixtures were stirred thoroughly and allowed to stand. After 2, 4, 6, 8, 10, 12, 18, 24, 36, 48, 60, and 72 hours of adding the samples to the artificial intestinal fluid, one sample was taken from each sample. The suspension samples were filtered using medium-speed filter paper, and the filter residue was dried using a rapid moisture analyzer. The mass of the filter residue was then weighed and recorded. The results showed that after 72 hours, the absorption rate of the cell-wall-removed Ganoderma lucidum spore powder was approximately 1.91 times that of the ordinary cell-wall-removed Ganoderma lucidum spore powder. (See attached figure). Figure 4 . Example 5

[0032] Preparation of cell wall-broken Ganoderma lucidum spore powder granules Take 50 grams of the cell-wall-removed and broken Ganoderma lucidum spore powder obtained in Example 1, atomize and spray it with 25 grams of purified water to make cell-wall-removed and broken Ganoderma lucidum spore powder granules, and granulate them through a 30-mesh sieve. After setting the hot air circulating oven to 55°C, dry the material until the moisture content is below 6%. Use a gyratory granulator to granulate through a 30-mesh sieve to obtain cell-wall-removed and broken Ganoderma lucidum spore powder granules. Example 6

[0033] Preparation of cell wall-broken Ganoderma lucidum spore powder capsules Take 50 grams of the cell-wall-removed and broken Ganoderma lucidum spore powder obtained in Example 1, atomize and spray it with 30 grams of 75% edible alcohol to form a soft mass, and granulate it through a 30-mesh sieve. Dry the material in a hot air circulating oven at 55°C until the moisture content is below 6%. After granulation using a gyratory granulator through a 30-mesh sieve, mix it evenly with 10 grams of microcrystalline cellulose. Fill the mixture into No. 0 gelatin capsule shells using a fully automatic capsule filling machine to obtain cell-wall-removed and broken Ganoderma lucidum spore powder capsules.

Claims

1. A cell wall-breaking Ganoderma lucidum spore powder, characterized in that, Take a 0.15g sample and dilute it to 100mL with purified water. After sonicating until the spores are fully dispersed, use a pipette to draw a drop of the middle layer liquid and place it on a hemocytometer. Cover it with a coverslip and let it stand for about 30 seconds after the sample is injected. Then place the hemocytometer under an 800x optical microscope for observation and counting. Randomly select 10 small squares and record the number of Ganoderma lucidum spore wall fragments in each small square. The average number of Ganoderma lucidum spore wall fragments per small square is no more than 5.

2. The method for preparing the cell wall-broken Ganoderma lucidum spore powder according to claim 1, characterized in that, Includes the following steps: a. Pretreatment: By rapidly lowering and raising the temperature of Ganoderma lucidum spore powder, the tissue connection between the Ganoderma lucidum spore wall shell and the Ganoderma lucidum spore kernel is destroyed; b. Pre-humidification: Spray the Ganoderma lucidum spore powder, which is about to undergo the cell wall breaking process, with a pre-humidification solution and stir evenly; c. Low-temperature shearing and cell wall breaking: The pre-moistened material is broken at low temperature using a Ganoderma lucidum spore powder cell wall breaking machine (based on the principle of double roller differential shearing and cell wall breaking) to obtain broken Ganoderma lucidum spore powder; d. Sedimentation and recovery of sedimentation liquid: The broken-cell wall Ganoderma lucidum spore powder obtained in step c is mixed evenly with a small amount of sedimentation liquid to prepare a broken-cell wall Ganoderma lucidum spore powder solution. This solution is then slowly poured into a sedimentation tank containing the sedimentation liquid to separate the Ganoderma lucidum spore walls and spore kernels. After the sedimentation process is complete, food-grade filter paper is used to cover the bottom of the sedimentation tank to recover the sedimentation liquid and obtain the stratified moist broken-cell wall Ganoderma lucidum spore powder. e. Drying: Dry the layered moist Ganoderma lucidum spore powder obtained in step d to obtain layered Ganoderma lucidum spore powder; f. Microscopic examination of layering: A conical sampling pit is dug on the layered Ganoderma lucidum spore powder obtained in step e. Samples are taken and examined under a microscope to confirm the boundary height between the Ganoderma lucidum spore wall shell layer and the cell wall-removed Ganoderma lucidum spore powder. The upper layer of Ganoderma lucidum spore wall shell layer is separated by hand shovel to obtain cell wall-removed Ganoderma lucidum spore powder.

3. The preparation method according to claim 2, characterized in that, In step a, rapid heating and cooling cycles of 1 to 3 times can disrupt the tissue connection between the Ganoderma lucidum spore wall and the Ganoderma lucidum spore kernel; rapid cooling is achieved using liquid nitrogen isolation cooling; and rapid heating is achieved using hot water isolation heating at 40℃ to 80℃.

4. The preparation method according to claim 2, characterized in that, In step b, the pre-humidification solution is purified water; the moisture content of the pre-humidified material ranges from 8% to 15%.

5. The preparation method according to claim 2, characterized in that, In step c, the cooling water in the chiller of the Ganoderma lucidum spore powder cell wall blender is a 10%-20% concentration of saline solution; the cooling water temperature is -5℃ to -15℃; and under working conditions, the surface temperature range of the double roller components of the Ganoderma lucidum spore powder cell wall blender is -2℃ to -10℃.

6. The preparation method according to claim 2, characterized in that, In step d, the settling liquid is water or 5%~75% edible alcohol; the material-to-liquid ratio is 1:5~10.

7. The preparation method according to claim 2, characterized in that, The drying temperature in step e is 35℃~70℃.

8. The preparation method according to claim 2, characterized in that, The microscopic examination method in step f is as follows: Take 0.15g of sample and dilute it to 100mL with purified water. After sonicating until the spores are fully dispersed, use a pipette to draw a drop of the middle layer liquid and place it on a hemocytometer. Cover it with a coverslip. After the sample is injected, let it stand for about 30 seconds. Then, place the hemocytometer under an 800x optical microscope for observation and counting. Randomly select 10 small squares and record the number of Ganoderma lucidum spore wall fragments in each small square. The average number of Ganoderma lucidum spore wall fragments per small square is no more than 5.

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