Compound with wrinkle removing function and application of compound

A compound and functional technology, applied in cosmetic preparations, preparations for skin care, pharmaceutical formulations, etc., can solve the problems of undisclosed triterpene saponins and triterpenoid acids, and achieve the effect of improving the repairing effect.

Inactive Publication Date: 2016-02-03
JIANGSU LONGLIQI BIOSCIENCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far it has not been disclosed that the complex of triterpene saponins and triterpene acids in Centella asiatica is used for repairing aging skin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Normal human skin fibroblasts were inoculated on cell culture plates at an appropriate density, digested with 0.25% trypsin, and then prepared into a single cell suspension with DMEM medium containing 10% fetal bovine serum by volume. solution, 1×10 per well 5 Inoculated in 96-well plates or cell culture dishes (30mm), adhered to the wall overnight, and discarded the supernatant. Then add DMEM medium without fetal bovine serum, starve for 24 hours, and synchronize the cells in each group, in 5% CO 2 , Cultivated in a 37°C incubator.

[0019] The experiment was divided into an untreated blank control group and 5 treatment groups, which were asiaticoside 50ug / ml in treatment group 1, madecassoside 50ug / ml in treatment group 2, and centella asiaticoside in treatment group 3. Glycoside 25ug / ml and madecassoside 25ug / ml, asiaticoside 25ug / ml and asiatic acid 25ug / ml in treatment group 4, asiaticoside 25ug / ml and madecassic acid 25ug / ml in treatment group 5 / ml, the fi...

Embodiment 2

[0022] According to the method for cell culture in Example 1, the experiment was divided into an untreated blank control group and 4 treatment groups, which were respectively asiatic acid 30ug / ml of treatment group 1 and 30ug of madecassic acid of treatment group 2 / ml, asiatic acid 15ug / ml and madecassic acid 15ug / ml of treatment group 3, asiatic acid 15ug / ml and asiatic acid 15ug / ml of treatment group 4, asiatic acid 15ug / ml of treatment group 5 ml and madecassoside 15ug / ml. All five treatment groups were formulated with DMEM medium without fetal bovine serum. After cell starvation treatment, active ingredients were added for treatment respectively, and a control group was set up, and 6 duplicate wells were set up respectively. After culturing for 48 hours, the supernatant was centrifuged, and the content of procollagen type I was determined according to the detection kit (ProcollagenTypeIC-Peptide (PIP) EIAKit) instructions, detect the content of type I procollagen in the ...

Embodiment 3

[0025] The following is the application of the complex of triterpene saponin and triterpene acid in cosmetics with remarkable anti-wrinkle function, which is formed by mixing each component according to conventional techniques.

[0026] Cosmetic formula 1: anti-wrinkle cream, by mass percentage, glycerin 6.0%, butanediol 3.0%, 1,2-pentanediol 2.0%, EDTA disodium 0.1%, PEG-100 stearate 1.5%, glycerin Stearate 0.5%, Caprylic / Capric / Myristic / Stearic Triglyceride 6.0%, Cholesteryl Hydroxystearate 1.0%, Cetyl Alcohol 2.0%, Shea Butter (BUTYROSPERMUMPARKII) 2.0% , Behenyl Alcohol 0.5%, Dimethicone 1.0%, Cholesterol 0.2%, Xanthan Gum 0.3%, Sodium Hyaluronate 0.05%, Phenoxyethanol 0.3%, Methylparaben 0.2%, Paraben Ethyl ester 0.1%, essence 0.1%, compound 0.1%, deionized water make up to 100%, wherein the mass ratio of triterpene saponin and triterpene acid in the compound is 3:1.

[0027]Cosmetic formula 2: anti-wrinkle lotion, by mass percentage, glycerin 6.0%, butanediol 5.0%, 1,2-...

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PUM

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Abstract

The invention discloses a compound with a wrinkle removing function. The compound comprises triterpenoid saponin and triterpene acid, wherein the triterpenoid saponin and the triterpene acid are active ingredients from centella. The triterpenoid saponin comprises one or two of asiaticoside and hydroxy asiaticoside, and the triterpene acid comprises one or two of asiatic acid and madasiatic acid. By compounding any one or two of the triterpenoid saponin with any one or two of the triterpene acid, human skin fibroblast secretes type I collagen, and the like can be remarkably improved, the obvious effect of aging skin regeneration is realized, and the compound can be used for the development of wrinkle removing cosmetics.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a compound with wrinkle-removing function and its application. Background technique [0002] Centella asiatica L. is a plant of the genus Centella asiatica in the Umbelliferae family, also known as Bengdawan, Callagrass, Gotu Kola, Tiger Grass, etc. It has been used in my country for more than 2,000 years. It is mainly used for clearing heat and dampness, reducing swelling and detoxification, damp-heat jaundice, carbuncle sores, bruises and other diseases. Modern pharmacological studies have shown that Centella asiatica extract has a strong central nervous system inhibitory effect and various pharmacological effects such as anti-gastric ulcer, anti-cancer, anti-inflammation, enhancing memory, improving cardiovascular and cerebrovascular, and promoting wound healing. The main active substances in Centella asiatica are triterpenoid saponins such as asiaticoside and madecassoside, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K8/63A61Q19/08
CPCA61K8/97A61K8/63A61Q19/08
Inventor 刘廷强段明星严泽民喻启波徐之伟
Owner JIANGSU LONGLIQI BIOSCIENCE CO LTD
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