Preservative-free polypeptide composition with anti-aging effect

A technology of polypeptide composition and carnosine, which is applied in the field of antiseptic polypeptide composition and its preparation, can solve the problems of limited efficacy and achieve the effect of promoting collagen production and fibroblast proliferation

Active Publication Date: 2020-12-08
杭州时光肌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a non-preservative polypeptide composition with components homologous to the

Method used

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  • Preservative-free polypeptide composition with anti-aging effect
  • Preservative-free polypeptide composition with anti-aging effect
  • Preservative-free polypeptide composition with anti-aging effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Add 0.20g oligopeptide-1, 0.25g carnosine, 0.25g tripeptide-1, 0.3g pentapeptide-3, 1.0g recombinant human type I collagen, 8g mannitol to 90g ultrapure water, stir until completely dissolved.

[0033] 2. Freeze the solution obtained in step 1 at -48°C for 5h, then vacuumize, and raise the temperature to -20°C, keep for 8h; continue to heat up to -10°C, keep for 5h; then warm up to 0°C, keep for 6h; continue The temperature was raised to 20° C. and kept for 5 hours; finally the temperature was raised to 40° C. and kept for 8 hours to obtain a freeze-dried powder, that is, a polypeptide composition.

[0034] The obtained polypeptide composition is added in different proportions to high-sugar DMEM culture solution containing 10% newborn calf serum, and human skin fibroblasts are cultured in vitro. Discard the culture medium after 48 hours, wash it, test it with a cell proliferation detection kit, record the absorbance value at 450nm, calculate the relative cell viabil...

Embodiment 2

[0038] 1. Add 0.24g oligopeptide-1, 0.24g carnosine, 0.16g tripeptide-1, 0.16g pentapeptide-3, 1.2g recombinant human type I collagen, 8g mannitol to 90g ultrapure water, stir until completely dissolved.

[0039] 2. Freeze the solution obtained in step 1 at -50°C for 6h, then vacuumize, raise the temperature to -30°C, and keep it for 10h; continue to heat up to -15°C, keep it for 6h; then raise the temperature to 0°C, keep it for 6h; continue to heat up to 20° C. and keep for 7 hours; finally raise the temperature to 40° C. and keep for 8 hours to obtain a lyophilized powder, that is, a polypeptide composition.

[0040] The polypeptide composition prepared in this example is added to the water agent to make anti-wrinkle firming toner, and the specific composition of the toner is shown in Table 1:

[0041] Table 1

[0042]

[0043]

[0044] The concrete preparation method of above-mentioned softening water is as follows:

[0045] 1. According to the proportions in Tabl...

Embodiment 3

[0049] 1. Add 0.3g oligopeptide-1, 0.25g carnosine, 0.25g tripeptide-1, 0.2g pentapeptide-3, 1.5g recombinant human type I collagen, 7.5g mannitol into 90g water, stir until completely dissolved .

[0050] 2. Freeze the solution obtained in step 1 at -45°C for 7h, then vacuumize, and raise the temperature to -25°C, keep for 6h; continue to heat up to -10°C, keep for 7h; then warm up to 0°C, keep for 8h; continue The temperature was raised to 20° C. and kept for 6 hours; finally the temperature was raised to 35° C. and kept for 8 hours to obtain a lyophilized powder, that is, a polypeptide composition.

[0051] Add the polypeptide composition prepared in this example to the cream to make anti-wrinkle firming lotion. The specific composition of the lotion is shown in Table 2:

[0052] Table 2

[0053]

[0054]

[0055] The concrete preparation method of above-mentioned emulsion is as follows:

[0056] 1. According to the proportions in Table 1, weigh the raw materials o...

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Abstract

The invention discloses a preservative-free polypeptide composition with an anti-aging effect. The preservative-free polypeptide composition is freeze-dried powder prepared from the following raw materials in percentage by mass of 1%-20% of compound polypeptide, 1%-20% of recombinant human I-type collagen and 60%-98% of a freeze-drying protective agent, wherein the compound polypeptide comprises the following components in percentage by mass of 15%-30% of oligopeptide-1, 20%-30% of carnosine, 5%-30% of tripeptide-1 and 10%-55% of pentapeptide-3. The polypeptide composition has a remarkable proliferation promoting effect on human skin fibroblasts, has a promoting effect on I-type collagen gene expression at high concentration has a promoting effect on III-type collagen gene expression at low concentration, and keeps the activity of peptide raw materials to the maximum extent; and meanwhile, the polypeptide composition does not contain preservative, so that the influence of the preservative on the activity and stability of the peptide components is avoided. The polypeptide composition disclosed by the invention can be added into different products such as water aqua, cream, masks andessences according to requirements, so that the anti-aging effect is provided for the products.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a non-corrosion polypeptide composition with anti-aging effect and a preparation method thereof. Background technique [0002] Anti-aging is a long-standing hot topic in the cosmetics industry. Early anti-aging products mostly used plant extracts as the main functional ingredients, but in recent years, more and more anti-aging products with bioactive peptides as the main ingredient have occupied the beauty market. [0003] Biologically active peptides refer to peptide compounds that are beneficial to the life activities of organisms or have physiological effects. Because of its diverse functions and high safety, it is considered to be one of the most popular raw materials in the cosmetics industry today, and it is widely used in anti-aging, whitening, repairing and other fields. [0004] There are many kinds of peptide / protein raw materials commonly used in the co...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61Q19/08A61Q19/00
CPCA61K8/64A61Q19/08A61Q19/00A61K2800/30
Inventor 张大勇罗惠敏侯亚东王国柱
Owner 杭州时光肌生物科技有限公司
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