Tissue engineering skin with layered structure and preparation method of tissue engineering skin

Abstract

The invention discloses tissue engineering skin with a layered structure and a preparation method of the tissue engineering skin, and relates to the technical field of tissue engineering skin. The tissue engineering skin with the layered structure comprises a collagen layer, a collagen layer embedded in human skin fibroblast, a first multilayer collagen layer, a collagen layer embedded in keratinocyte and a second multilayer collagen layer. According to the tissue engineering skin disclosed by the invention, a manner that channels are innovatively generated in tissues by a temporary material such as gelatin is adopted, and human umbilical vein endothelial cells are adhered to the inner parts of the channels, so that the formed blood vessels are better fit with human body blood vessels; thetransporting and distributing rate of the formed blood vessels is increased; cells have higher viability and high multiplication rate; the printed skin with the layered structure is adopted, so thatthe interaction between cells of the formed skin structure and the interaction of the skin tissues and epimatrix are more stable, and regeneration of the skin tissue is facilitated.

Description

technical field [0001] The invention relates to the technical field of tissue engineering skin, in particular to a tissue engineering skin with layered structure and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, an important tissue covering the body surface, and a barrier between the human body and the external environment. It not only undertakes functions such as protecting the body, sweating, and sensing, but also maintains the stability of the internal environment of the body. Skin repair is important in the treatment of burns, lacerations and diabetic wounds. Typically, in order to treat skin lesions, grafts of healthy skin from other parts of the body are needed, however, extensive skin lesions require a large amount of skin available for grafting, and this approach not only creates new wounds in the donor site , also has defects such as limited source of normal skin itself and low survival rate of grafted ...

AI Technical Summary

Problems solved by technology

However, this method is easily limited by the shape of the repair site, and it is difficult to produce a skin shape that fits the repair site when organotypic skin culture is required for the purpose of wound repair, and it is not easy to produce it easily by conventional methods.

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