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Lumbricus peptide capable of resisting skin aging as well as preparation method and application thereof

An anti-skin aging and earthworm peptide technology, applied in the biological field, can solve the problems of excessive molecular weight of the target peptide, complicated preparation and separation process, and poor pertinence, and achieves strong affinity, obvious antioxidant activity, and safety. high effect

Active Publication Date: 2014-06-11
KARLIE COSMETICS MFG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of earthworms to prepare active peptides for the development of anti-aging skin products, and in the process of using earthworms as raw materials to develop and prepare peptides, there are still complicated preparation and separation processes, excessive molecular weight and wide distribution of target peptides. Issues such as poor pertinence

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preparation method of dilongide is provided, comprising the steps of:

[0018] (1) Wash the fresh earthworms, put them in deionized water or pure water for 24 hours, so that the food residues in the earthworms can be discharged from the body, and then use a high-speed masher to crush the washed earthworms, and the earthworm crushing liquid is fresh Earthworm sample liquid; Wherein said fresh earthworm is P. aspergillum (E.Perrier)), common ringworm (P.vulgaris Chen), William ringworm (P.guillelmi (Michaelsen) )), Pectinifera (Michaelsen), P.hupeiensis, P.tschiliensis, P.diffringens, P. One or more of P.californica, Allolobophora caliginosa trapezoides, or Eisenia foetida.

[0019] (2) Add 2 times the volume of deionized water to the earthworm sample solution, and use its own enzymes to hydrolyze. The enzymatic hydrolysis conditions are: pH value 6.5, temperature 40 ° C, hydrolysis 3h, that is, hydrolyzed hydrolyzate rich in dilongin liquid.

[0020] (3) The hydroly...

Embodiment 2

[0022] A preparation method of dilongide is provided, comprising the steps of:

[0023] (1) Wash the fresh earthworms and put them in deionized water or pure water for 36 hours to make the food residues in the body of the earthworms exhausted, and then use a high-speed masher to crush the washed earthworms, and the crushed earthworms are fresh Earthworm sample solution;

[0024] Dried earthworms are mashed, then soaked in deionized water or purified water with 5 times the mass volume, and the soaking solution is the earthworm sample liquid;

[0025] (2) Fresh earthworm sample solution and dry earthworm (Dilong) sample solution were mixed according to the volume ratio of 2:1, and hydrolyzed by their own enzymes. The enzymatic hydrolysis conditions were: pH value 6.5, temperature 45°C, hydrolysis 6h, that is A hydrolyzate rich in dilongide was obtained.

[0026] (3) The hydrolyzate is filtered through an ultrafiltration membrane with a molecular weight cut-off of 3000 Daltons,...

Embodiment 3

[0028] A preparation method of dilongide is provided, comprising the steps of:

[0029] (1) mash the dry earthworm product, then soak it in deionized water or pure water with 5 times the mass volume, and the soaking solution is the earthworm sample liquid;

[0030] (2) 50ml of dry earthworm sample solution, add 0.5mg of neutral protease with an enzyme activity unit of 12000U / mg, and carry out enzymatic hydrolysis. The hydrolyzate of dilongide.

[0031] (3) The hydrolyzate is filtered through an ultrafiltration membrane with a molecular weight cut-off of 3000 Daltons, and the filtrate is intercepted by a nanofiltration membrane with a molecular weight of about 300, and the retained liquid is freeze-dried to obtain dilongide.

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PUM

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Abstract

The invention discloses a lumbricus peptide capable of resisting skin aging as well as a preparation method and an application thereof. The preparation method comprises the following steps: hydrolyzing an earthworm sample liquid by using own enzyme or adding protease, then carrying out membrane filtration and membrane interception on the hydrolysate, and freezing and drying to acquire the lumbricus peptide which can be applied to cosmetics or health care products capable of resisting skin aging. By the way, the preparation method is green and simple, and the prepared lumbricus peptide has the advantages of strong affinity, high safety, obvious antioxidant activity and capabilities of effectively promoting growth and multiplication of human skin fibroblast, significantly promoting secretion and synthesis of cell matrix collagen and obviously resisting skin aging and can be applied to the development of cosmetics and health care products capable of resisting skin aging.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kind of anti-aging dilong peptide and its preparation method and application. Background technique [0002] Peptides are a class of biologically active substances involved in various cellular functions in organisms, and participate in the regulation of various complex physiological processes in the human body. Peptide has the characteristics of high activity, strong affinity, and high safety, and the peptide molecule is nano-scale, which is easily absorbed by the human intestine, or directly enters the deep skin through the surface layer of the skin, and is quickly absorbed by the human body to play a role. Modern pharmacological studies have shown that some active peptides not only have antioxidant activity, but also can effectively promote the growth and proliferation of human skin fibroblasts, especially by increasing the activity of skin fibroblasts, effectively promoting the g...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/64A23L1/29A61Q19/08A23L33/00
Inventor 刘廷强周华锋侯冬梅严泽民段明星徐之伟
Owner KARLIE COSMETICS MFG CO LTD
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