198 results about "Peptide molecule" patented technology

The invention discloses a bidirectional reversible conversion method and system between a peptide molecule SMILES and a sequence expression. The core innovation lies in that a new sequence description syntax is defined to retain information of a polypeptide special bond and specific modification of amino acid; a main chain atom index and adjacency traversal topology identification algorithm is adopted, and end group and topology integrated detection and coding are carried out; a residue recognition algorithm for main chain cutting and template library matching is compatible with any standard or non-standard amino acid residues, an extensible end group library / monomer template library and an automatic increment mechanism, and automatic recognition and sequence annotation of S-S disulfide bonds; the invention relates to a high-fidelity assembly algorithm of HELM anchor points and topology aware cyclic peptide processing. The method solves the problems of incapability of supporting a complex polypeptide topological structure, poor reversibility, insufficient expansibility of a monomer library and the like in the prior art, can be widely applied to scenes of quantitative structure-activity relationship model construction, large-scale polypeptide data cleaning and the like, and has remarkable practicability and innovativeness.

The present application provides a soybean antihypertensive peptide and its screening method and application, which belongs to the field of polypeptides. The soybean antihypertensive peptide provided in the present application includes one or more of soybean antihypertensive peptide I with a molecular weight of less than 1000Da, soybean antihypertensive peptide II with a molecular weight of less than 1000Da, and soybean antihypertensive peptide III with a molecular weight of less than 1000Da; wherein, the soybean antihypertensive peptide I with a molecular weight of less than 1000Da is prepared by nattokinase enzymatic hydrolysis of soybean protein isolate, the soybean antihypertensive peptide III with a molecular weight of less than 1000Da is prepared by Bacillus subtilis liquid fermentation of soybean protein hydrolysate, and the soybean antihypertensive peptide III with a molecular weight of less than 1000Da is prepared by Bacillus subtilis solid fermentation of soybeans. The soybean antihypertensive peptide of the present application is a plant-derived antihypertensive peptide with high angiotensin converting enzyme inhibition rate and good antihypertensive effect. And it has been verified by animal experiments that the soybean antihypertensive peptide of the present application can significantly reduce the heart rate, diastolic blood pressure and systolic blood pressure of rats, and has less toxic and side effects.

The present invention relates to a polypeptide CYS3 that affects seed germination. The CYS3 polypeptide molecule affects the germination of immature tobacco seeds. Immature tobacco seeds picked 20 days in advance after pollination are used as test materials. The CYS3 polypeptide is expressed and then treated in the tobacco seeds. It is found that the CYS3 protein has a germination-promoting effect on the immature tobacco seeds, providing a practical technology for promoting tobacco seed germination and seedling emergence in production.

The embodiment of the invention relates to a phycobiliprotein active peptide with a hypoglycemic function and a preparation method and application thereof, and belongs to the technical field of biological active peptides. According to the phycobiliprotein active peptide with the blood sugar reducing function, the amino acid sequence of the phycobiliprotein active peptide is Ser-Tyr-Phe-Asp-Arg-Ala, Ile-Ala-Ser-Tyr-Phe-Asp-Arg, Gly-Tyr-Tyr-Leu-Arg or Val-Ala-Phe-Gly-Arg-Phe, and the amino acid sequence of the phycobiliprotein active peptide with the blood sugar reducing function is shown in the description. The phycobiliprotein active peptide in the embodiment of the invention is separated from spirulina phycobiliprotein, and the spirulina phycobiliprotein is an important protein resource in spirulina and a high-quality source of bioactive peptide, and has wider application; the phycobiliprotein active peptide is low in molecular weight, and compared with a chemically synthesized hypoglycemic preparation, food-borne peptides are safer and more efficient, free of toxic and side effects and easy to digest and absorb; the phycobiliprotein active peptide has high binding affinity with the alpha-glucosidase and the DPP-IV, can form a very stable ligand receptor compound, and has a good inhibition effect on the alpha-glucosidase and the DPP-IV; the compound has excellent inhibition performance on alpha-glucosidase, alpha-amylase and DPP-IV (dipeptidyl peptidase-IV).

The invention relates to the technical field of collagen preparation, in particular to a collagen product as well as a preparation method and application thereof. The collagen product prepared by the invention overcomes the problems of poor dissolution effect in cold water, poor fat solubility and poor emulsifying property. The preparation method comprises the following steps: crushing bovine bones, and performing collagen extraction, enzymolysis treatment, glycosylation treatment, fatty acid amidation treatment, polyphenol grafting modification and colloid compounding to obtain the collagen product. During glycosylation treatment, the cold water dissolving effect is improved by adjusting the variety of reducing sugar, the reaction temperature and time and grafting bone peptide molecules to sugar chains, and a flavor substance is generated by the Maillard reaction in the glycosylation treatment, so that the effect of covering the fishy smell of cattle bones is achieved; relevant parameters are adjusted through fatty acid amidation treatment, the bone peptide is endowed with liposolubility and emulsibility, and the solubility and emulsibility of the collagen product are synergistically improved through colloid compounding, optimization of the use amount of various colloids and control of reaction conditions.

The invention belongs to the technical field of biological fermentation, and particularly relates to ganoderma lucidum polypeptide and a preparation method and application thereof. The preparation method comprises the following steps: (1) taking ganoderma lucidum powder, adding water, and cooking to obtain a mixture 1; (2) adding bacillus subtilis liquid into the mixture 1, and fermenting to obtain ganoderma lucidum polypeptide fermentation liquid bacteria liquid; (3) adding neutral protease into the ganoderma lucidum polypeptide fermentation liquid for primary enzymolysis, and then adding aminopeptidase for secondary enzymolysis to obtain ganoderma lucidum polypeptide enzymatic hydrolysate; and (4) filtering, concentrating and spray-drying the lucid ganoderma polypeptide enzymatic hydrolysate to obtain the lucid ganoderma polypeptide. The molecular weight of the ganoderma lucidum polypeptide ranges from 200 Da to 5000 Da, molecular weight distribution is uniform, the polypeptide content can reach 31.11% at most, the ganoderma lucidum polypeptide can be applied to functional cosmetics, and the application range of the ganoderma lucidum polypeptide is widened.

The colla corii asini low-peptide molecule generator comprises a supporting plate and a motor, a rotating mechanism is arranged at one end of the side face of the motor, and an enzymolysis tank used for mixing a colla corii asini aqueous solution and enzymes is arranged at one end of the side face of the rotating mechanism; one end of the side surface of the enzymolysis tank is provided with an air extracting mechanism for extracting air from the interior of the enzymolysis tank, and a first connecting pipe and a second connecting pipe for air circulation are arranged between the air extracting mechanism and the enzymolysis tank; according to the device, oxygen in the enzymolysis tank can be pumped out through the air pumping mechanism when an oxygen-free environment is needed in the enzymolysis process, the enzymatic activity is protected, the enzymolysis efficiency can be better improved, then a donkey-hide gelatin aqueous solution and enzymes in the enzymolysis tank can be more uniformly mixed through the rotating mechanism, the enzymolysis tank and the like, and the enzymolysis efficiency is improved. The enzymolysis efficiency is improved, and the quality of protein is ensured.

The invention relates to the field of biological medicine and aquatic products, and discloses a grass carp IFNf interferon and truncated protein and application thereof in virus resistance or bacterium resistance. The applicant discovers the new subtype IFNf of the grass carp interferon for the first time, the protein coded by the new gene has an efficient antiviral effect, and the derived peptide AMP-G2 of the grass carp interferon has broad-spectrum antibacterial activity. The sensitivity of the grass carp interferon IFNf to grass carp reovirus (GCRV) infection is higher than that of other interferon, and the grass carp interferon IFNf shows obvious antiviral activity on the cellular level and can be used for preparing antiviral drugs. The interferon-derived peptide provided by the invention is a host natural peptide, is small in molecular weight and simple to synthesize, has a remarkable bactericidal effect on various bacteria and fungi, and can be used for preparing broad-spectrum antibacterial agents or antibacterial drugs.

Provides herein are multifunctional polypeptide molecules comprising T cell receptor variable alpha-binding moieties, T cell receptor variable beta-binding moieties, TAA-binding moieties, and / or cytokines, and methods of treating conditions or diseases in a subject using the same.

The invention relates to the technical field of fluid circulation of collagen peptide production equipment, in particular to a collagen peptide mechanical seal water circulation system which comprises an intelligent control unit, a cooling unit and an ultrasonic descaling unit. Converting the vibration spectrum into a peptide molecule aggregation tendency coefficient, correcting a viscosity predicted value to generate a flow control weight, allocating resources to a cooling unit according to a hydrolysis risk thermodynamic diagram, directionally cooling a high-risk area, activating an ultrasonic descaling unit and reducing the flow when the aggregation coefficient continuously rises and exceeds a limit, the system generates cooperative control quantity through rolling of a three-dimensional dynamic weight matrix, synchronously adjusts the rotating speed of a water pump, the opening degree of a cooling valve and ultrasonic power, solves the problems of unstable flowing, peptide chain hydrolysis and aggregation scaling, and guarantees production stability and product quality.

To provide: a method for producing a complicated peptide molecule, such as a dimeric peptide, in a simpler manner at lower cost compared with the conventional chemical synthesis methods; a dimeric peptide which comprises a specific short linker and has a satisfactory activity; and others.SOLUTION: A peptide molecule comprises: (I) a first physiologically active peptide portion; (II) a linker portion composed of 49 or less amino acid residues; and (III) a second physiologically active peptide portion located on the opposite side of the linker portion from the first physiologically active peptide portion, wherein the first physiologically active peptide portion and the second physiologically active peptide portion may be the same as or different from each other, and at least 90% of an amino acid sequence of the linker portion is composed of amino acid residues selected from alanine (A), proline (P), and serine (S).SELECTED DRAWING: None

The invention discloses an Al / polypeptide / AP biological composite energetic material and a preparation method thereof. The biological composite energetic material is of a core-shell structure with Al / polypeptide as a core and AP as a shell. The particle size of the biological composite energetic material is 5-300 [mu] m; the content of the aluminum powder is 20-45% by mass, the polypeptide is 0-5% by mass, the AP is 50-75% by mass, and the content of the polypeptide is not 0%. The invention further provides a preparation method of the Al / polypeptide / AP biological composite energetic material, a cooling recrystallization method is adopted, the self-assembly characteristic of polypeptide molecules is combined, directional arrangement of Al, polypeptide and AP on the nanoscale is achieved, and a uniformly-mixed composite material is formed. The material is stable in structure, the Al powder is not easy to agglomerate, hydroxyl, carboxyl and nitro functional groups in the polypeptide respectively form strong interaction with the Al powder and AP, and the mechanical property, the thermal stability and the reaction activity of the material are remarkably improved. In addition, the preparation process is simple and convenient, the negative influence on the environment is reduced due to the biodegradability of the polypeptide, and the method is suitable for large-scale industrial production.

The application relates to the fields of biological medicine and aquaculture, and discloses a zebrafish IFNf interferon truncated protein and application thereof in antibiosis. The zebrafish interferon derivative peptide AMP-Z2 can specifically inhibit fungi. The interferon derivative peptide provided by the application is a host natural peptide, has small molecular weight, is simple to synthesize, has significant bactericidal effects on various bacteria and fungi, and can be used for preparing broad-spectrum antibacterial agents or antibacterial drugs.

The invention belongs to the technical field of biochemistry and functional polymer materials, and particularly relates to a polysaccharide-oligopeptide co-assembled intestinal flora regulation and control material and a preparation method thereof. The material is prepared from imine-boric acid ester double dynamic crosslinking modified chitosan, glycyl-tryptophan dipeptide, sodium lactate, citric acid, glycerol and a phosphate buffer solution. A reversible imine bond and a borate bond are introduced into chitosan molecules to construct a polysaccharide network with a double-dynamic crosslinking characteristic, and then the polysaccharide network and oligopeptide molecules are synergistically self-assembled to form a three-dimensional gel structure. The material has pH responsiveness, self-repairing property and slow-release function, and can realize continuous release of short peptides and selective regulation and control of probiotics in an intestinal environment. The prepared material is high in gel strength, good in structural stability and stable in oligopeptide release, and has remarkable promotion and inhibition selectivity on intestinal flora. The method provides an innovative co-assembly strategy for polysaccharide functionalization and micro-ecological intervention, and has a good application prospect.

The present invention relates to a method for the acute treatment of a relapsing-remitting condition, the method comprising the step of administering to a subject in need thereof one or more doses of an effective amount of a peptide molecule as defined in claim 1 in response to or during a relapse, wherein the method causes remission of the condition.

The present invention relates to the field of biotechnology. The present invention relates to a bioactive polypeptide, which has the effect of protecting the liver. Specifically, the present invention provides a bioactive polypeptide molecule PGPIP derived from A2β-casein in cow's milk, and its amino acid sequence is Pro-Gly-Pro-Ile-Pro. The present invention has confirmed through in vitro and in vivo experiments that it can effectively reduce liver lipid accumulation and hepatocyte damage, providing a feasible direction for the treatment of liver diseases, especially the prevention and treatment of alcoholic fatty liver; this bioactive polypeptide also has great application potential in the fields of food and health products.

The application belongs to the field of pharmacy, and particularly relates to a whole-process targeting polypeptide molecule with the functions of targeting brain capillary endothelial cells, tumor neovascular endothelial cells, tumor pseudo vascular, tumor cells and tumor stem cells, a stable and optimized polypeptide molecule, and a modified complex and drug delivery system for tumor diagnosis and treatment. The application prepares a whole-process targeting polypeptide WVAP and a WVAP modified drug and high polymer carrier material, and applies the WVAP to the construction of a drug delivery system for tumor imaging and targeted therapy. The results show that the WVAP can cross the blood-brain barrier, target tumor neovascular and cross the blood-tumor barrier, target tumor pseudo vascular, tumor cells and tumor stem cells; and the nano drug delivery system constructed by the WVAP modified high polymer carrier material can effectively deliver the loaded drug to the brain, target tumor tissues, and significantly improve the anti-tumor efficacy.

The application relates to the technical field of organic synthesis, and discloses a tyrosine compound olefination reaction method based on ligand promotion. The application successfully modifies tyrosine compounds by using compounds containing ethenyl as terminal groups by taking tyrosine as an embedded guiding group and by optimizing reaction raw materials and reaction conditions, and provides a method for C-H functionalization modification and reaction of polypeptide macromolecules. The reaction provided by the application can be carried out as long as the peptide molecule contains tyrosine, and tyrosine does not need to be used as a terminal residue of the peptide molecule, so that the olefination reaction method provided by the application has a wide application range.

The present invention relates to bispecific polypeptide molecules capable of simultaneously binding to a disease associated antigen and a cell surface antigen expressed on the surface of an immune effector cell such as a T-cell resulting in the activation of the immune effector cell.

The present invention provides compositions comprising peptide-coupled biodegradable poly(lactide-co-glycolide) (PLG) particles. In particular, PLG particles are surface-functionalized to allow for coupling of peptide molecules to the surface of the particles (e.g., for use in eliciting induction of immunological tolerance).

The invention relates to a hydrophobic tripeptide molecule, a crystal constructed by the hydrophobic tripeptide molecule and application of the hydrophobic tripeptide molecule in a piezoelectric device. The structural formula of the polypeptide molecule is shown as a formula (I). The polypeptide molecule can form a micron-sized crystal through a heating-cooling method, and the interior of the micron-sized crystal is of an asymmetric structure and is of a triclinic system. A crystal formed by the polypeptide molecule has a relatively high piezoelectric coefficient (21.5 pC / N), a sandwich type piezoelectric device is constructed by using the crystal, and the output voltage can reach 2.57 V, which is the highest value of an oligopeptide nano material prepared in an aqueous solution. Therefore, the invention provides a method for forming the tripeptide crystal, and provides a potential strategy for development of piezoelectric materials based on short peptide self-assembly. (I)

The invention belongs to the technical field of biological medicines, and particularly provides a brand-new active antialcoholism peptide, the sequence of which is as shown in SEQ ID NO.1; the invention also correspondingly provides a composition containing the peptide, and the composition can be a medicine, a health-care product or food. The peptide is small in molecular weight, easy to synthesize, safe, free of toxic and side effects and good in safety and practicability.

The invention provides royal jelly polypeptide CMKI-21 capable of promoting wound healing and resisting skin photoaging and application of the royal jelly polypeptide CMKI-21, and belongs to the technical field of biological medicine. The amino acid sequence of the royal jelly polypeptide is KKELVREVGFEICFKDHHEKI, and the royal jelly polypeptide is obtained by a solid-phase chemical synthesis method. The royal jelly polypeptide CMKI-21 disclosed by the invention is small in molecular weight, simple and feasible in preparation method, high in purity and good in biocompatibility, and can remarkably promote proliferation and migration of HaCaT cells, accelerate the healing rate of full-thickness skin defect wounds of mice, relieve light damage of the HaCaT cells and relieve skin light aging and sunburn caused by ultraviolet (UVB). The royal jelly polypeptide has the potential to be applied to various clinical skin wounds as a wound healing agent, and can also be applied to medicines and cosmetics for resisting skin photoaging and repairing sunburn.

The invention relates to the field of biological medicine, and provides a glycopeptide molecule, the glycopeptide molecule is a glycopeptide molecule or a glycopeptide assembly which is constructed by combining oligosaccharide fragments with oligopeptide fragments and has an osteogenesis promoting function, and the glycopeptide molecule has the effects of being safer, small in side effect and capable of effectively preventing and treating osteoporosis.

The present invention relates to the field of biomedicine and antibody engineering. Specifically, it relates to mono and multispecific multivalent single chain polypeptide molecules and derivatives thereof, preferably to multispecific single chain (tandem) trimerbodies with defined stoichiometry, to nucleic acid sequences and vectors encoding thereof, and host cells expressing the same. It further relates to methods of producing thereof, pharmaceutical compositions, kits, methods of treatment, use as diagnostics or imaging reagents and combination therapies using thereof.

The invention belongs to the technical field of protein beverages, and particularly relates to a composite peptide beverage and a preparation process thereof. The active ingredients of the drink comprise calf thymosin, cattle spleen protein peptide, hemoglobin peptide, bone collagen peptide and collagen peptide in a weight ratio of (2-3): 1: 1: 1: 1. The pH value of the system is adjusted to 3.5-4.5, so that each peptide molecule is positively charged under the acidic condition, the electrostatic repulsion and hydration among molecules are enhanced, and bitter groups are effectively masked. The flavoring agent is compounded by DL-malic acid, crystalline fructose, xylitol and sucralose according to the weight ratio of (0.6-1): 1: 0.4: (0.03-0.04), and the sour and sweet taste and flavor coordination are synergistically improved. The preparation method comprises the following steps: dissolving the DL-malic acid in purified water, dissolving the uniformly mixed active components in purified water at 40-60 DEG C, respectively adding other flavoring agents, stabilizing agents and essence, and finally filtering to obtain the product. The problem of bad flavor of the peptide is solved, so that the beverage is appropriate in taste, harmonious in flavor and easy to accept.

The invention discloses a preparation process optimization system and method of soybean enzymolysis protein peptides, and relates to the technical field of bio-enzymes, initial enzymolysis condition evaluation is carried out on soybean protein raw materials, an initial enzymolysis parameter combination is obtained, an experimental enzymolysis reaction is carried out, a hydrolysis degree change curve is obtained, whether the hydrolysis degree increase rate reaches a preset threshold value or not is judged, and if yes, the soybean enzymolysis protein peptides are obtained. If the peptide molecular weight distribution meets the target range, continuing the enzymolysis reaction, and when the peptide molecular weight distribution meets the target range, separating and purifying an enzymolysis product, recording a final enzymolysis parameter combination, judging whether a preset functional index is met or not, and if so, taking the final enzymolysis parameter combination as an optimized preparation process; if not, dynamically adjusting a preset threshold value or a target range; and if not, adjusting parameters in the initial enzymolysis parameter combination to form an optimized enzymolysis parameter combination. The functional activity and the stability of a protein peptide product are remarkably improved, and the production cost is reduced.

The application discloses an immunoregulatory peptide BugaCATH and application thereof, and belongs to the field of biomedicine. Buga The CATH is a cyclic polypeptide with a pair of intramolecular disulfide bond composed of a sixth cysteine and a thirteenth cysteine, and has a molecular weight of 3156.67 Dalton and an isoelectric point of 8.76, wherein the amino acid sequence is shown as SEQ ID NO: 2. Buga The gene (GenBank accession: OQ870533) of the CATH precursor is composed of 663 nucleotide sequences, and the nucleotide sequence is shown as SEQ ID NO: 1; wherein the nucleotide at the positions of 322-448 is the immunoregulatory peptide Buga The encoding gene of the CATH. The immunoregulatory peptide Buga The application of the CATH in the preparation of a treatment drug for promoting skin wound repair.

This invention discloses an environmentally friendly curcumin nanocrystal and its preparation method, relating to the field of nanoparticle technology. The nanocrystal has a core-shell structure, with a core of curcumin crystals and a shell of hepatin peptide molecules bonded together by intermolecular forces. The average particle size of the nanocrystal is 50-200 nanometers, and it is completely dispersed and stable in an aqueous phase, containing no organic solvents or synthetic surfactants. This invention utilizes a pectin-chitosan complex to microencapsulate the nanocrystal, enabling it to resist gastric acid degradation and target release to the colon, thus precisely acting on the gut-hepatic axis and significantly improving bioavailability and hepatoprotective efficacy. While completely avoiding organic solvents and synthetic stabilizers, it simultaneously solves four major industry challenges related to curcumin: solubility, stability, targeted delivery, and synergistic efficacy, providing a new technical path for developing high-end, naturally derived hepatoprotective products.