5results about How to "Method green" patented technology

The invention relates to a yeast gene modified cell containing GDP-fucose synthase polypeptide, which can be applied to synthesis of human milk oligosaccharide, and belongs to the technical field of gene engineering. According to the technical scheme, the yeast gene modified cell contains a heterologous nucleic acid sequence of a GDP-fucose synthase active polypeptide. The synthetase active polypeptide is obtained by modifying at least one site of the 20th site to the 29th site, the 40th site to the 53th site, the 75th site to the 81st site, the 95th site, the 148th site to the 158th site, the 193th site, the 215th site, the 219th site to the 21st site, the 227th site to the 232 site, the 239th site to the 241st site, the 259th site to the 264th site, the 301st site to the 302 site, the 306th site and the 310th site to the 316th site of an amino acid sequence shown in SEQ ID NO: 1. Compared with an original strain, the yeast gene modified cell has higher activity of synthesizing fucosyllactose, and a safe and feasible technical scheme is provided for industrial production of fucosyllactose.

The present application relates to a kind of preparation method of immunoglobulin powder and its application, the preparation method includes the following steps: collecting bovine colostrum, using cell survival technology is frozen;Frozen bovine colostrum is thawed, carries out milk fat separation, and obtains defatted bovine colostrum;Defatted bovine colostrum and composite rennet are mixed and are curdled, and then broken, obtain whey;The composite rennet includes aspartic protease, papain and bromelain;Whey is ultrafiltrated, and immunoglobulin solution is obtained;Immunoglobulin solution is vacuum freeze-dried, and the immunoglobulin powder is obtained.The preparation method provided by the present application is low temperature all the way, the activity of immunoglobulin is retained to the greatest extent, the use of composite rennet solves the problem of curd failure, and each link cooperates to improve the curd effect, realizes the preparation of high-efficiency, high-purity and high-quality immunoglobulin powder.

The present application relates to a kind of ferulic acid cyclodextrin inclusion compound and its preparation method and a kind of method for enhancing the water retention of muscle fiber protein hydrogel, belong to meat protein processing technical field.The preparation method of the ferulic acid cyclodextrin inclusion compound of the present application is, ferulic acid solution is mixed with cyclodextrin solution, and mixed solution is obtained;Mixed solution is dried, and cyclodextrin inclusion compound is obtained, solve the problems such as low water solubility of ferulic acid, poor stability etc.The present application also provides a kind of method for enhancing the water retention of muscle fiber protein hydrogel, specific method is, muscle fiber protein solution is mixed with ferulic acid cyclodextrin inclusion compound, heat treatment, by adding ferulic acid cyclodextrin inclusion compound, the water retention of muscle fiber protein gel is significantly improved.Therefore, the present application provides a kind of processing method of meat product with excellent quality, nutritional health and can be widely applied.

This invention provides a method and system for producing cyclohexyl hydrogen peroxide by cyclohexane oxidation. The method includes the following steps: reacting cyclohexane with an oxygen-containing gas containing CO2 to obtain a material containing cyclohexyl hydrogen peroxide. The method of this invention improves the yield and selectivity of cyclohexyl hydrogen peroxide by reacting the feed cyclohexane with an oxygen-containing gas containing CO2, reduces production costs, requires less equipment investment, and has a simple operation process. It can be used in the continuous industrial production of cyclohexane to cyclohexyl hydrogen peroxide via air oxidation.

This invention discloses a bamboo fiber, its preparation method, and a bamboo fiber wound hydrogen storage tank. The preparation method of the bamboo fiber includes the following steps: cutting natural bamboo into equal-length bamboo poles and pretreating them by soaking them in boiling water to remove water-soluble organic matter; mixing hydrogen peroxide, organic acid, and H2SO4 in a certain proportion to prepare a peroxyacid solution, wherein the concentration of hydrogen peroxide in the peroxyacid solution is 7-24 wt%, the concentration of organic acid is 15-55 wt%, and the concentration of H2SO4 is 0.5-3 wt%; soaking the pretreated bamboo poles in the peroxyacid solution at 50-60℃ for 12-20 hours to obtain bamboo fiber; then soaking the bamboo fiber in an alkaline solution for neutralization, and washing it after neutralization; vacuum drying the washed bamboo fiber and twisting it to extend its length to obtain long fiber; and applying microwave irradiation to the long fiber to obtain the final product.