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103 results about "Rennet" patented technology

Rennet /ˈrɛnɪt/ is a complex set of enzymes produced in the stomachs of ruminant mammals. Chymosin, its key component, is a protease enzyme that curdles the casein in milk. In addition to chymosin, rennet contains other important enzymes such as pepsin and a lipase.

Bacillus amyloliquefaciens and method for producing chymosin by fermenting using same

The invention relates to Bacillus amyloliquefaciens and a method for producing chymosin by fermenting using the same and belongs to the technical field of microbial fermentation. The invention discloses a bacterial strain for producing the chymosin. The bacterial strain is named as Bacillus amyloliquefaciens JUN002 and is collected in China Center for Type Culture Collection, wherein the collection number is CCTCC No:M2011045. The method for producing the chymosin by using the bacterial strain through liquid fermentation comprises the following steps of: performing slant culture; (2) performing seed culture; and (3) performing liquid fermentation culture, namely inoculating cultured seeds into a fermentation culture medium according to the inoculation quantity of 0.1 to 0.2 percent to culture the seeds at the temperature of between 32 and 36 DEG C for 60 to 72 hours. The Bacillus amyloliquefaciens and the method for producing the chymosin by fermenting using the same have the advantages that: the chymosin is produced by using the Bacillus amyloliquefaciens CCTCC No:M2011045 through liquid fermentation, so the enzyme production level is high, the fermenting time is short, growth conditions are easy to control and the like; and the method is completely suitable for application to industrialized production.
Owner:四川生力源生物工程有限公司

Red date wolfberry fruit yoghourt and making method thereof

InactiveCN104522170AStay Nutritious and TastyHas health benefitsMilk preparationMilk proteinVegetable Proteins
The invention relates to red date wolfberry fruit yoghourt and a making method of the red date wolfberry fruit yoghourt. According to the technical scheme, fresh milk is filtered, purified and cooled to 0 DEG C-5 DEG C, then passes through a purified milk separator to separate out fat and is proportionally added into skimmed milk to be prepared into standard milk; fresh red dates are added according to a formula and subjected to variable temperature and pressure difference puffing drying and superfine grinding to obtain red date powder, and the fresh wolfberry fruits are subjected to variable temperature and pressure difference puffing drying and superfine grinding to obtain wolfberry fruit powder, a stabilizer and a sweetener; ultrahigh-temperature short-time sterilization is carried out for 4 s-6s at the temperature of 137 DEG C, the product is cooled to the temperature of 34 DEG C-40 DEG C, 1.2 g/L-3.5 g/L chymosin is added, the product is inoculated with a fermentation agent and fermented for 8 h-16 h at the temperature of 35 DEG C-45 DEG C, and then the red date wolfberry fruit yoghourt is obtained. The problem that vegetable protein and milk protein are difficult to combine is solved by a modern scientific and technological means, the red dates and the wolfberry fruits high in nutrition value and healthcare value are added into the milk and fermented through the fermentation agent, and then the red date wolfberry fruit yoghourt with the high healthcare function is produced.
Owner:QINGHAI TIANYUAN DAIRY

Quark base mix having enhanced taste properties iii

A quark base mix is proposed having enhanced taste properties which is obtainable in that
  • (a) raw milk is subjected to a temperature treatment and the cream is separated off,
  • (b) the resultant skimmed milk is subjected to an ultrafiltration and in this case a first retentate R1 which contains a milk protein concentrate is produced, and a first permeate P1 is produced,
  • (c) the first permeate P1 is subjected to a nanofiltration and/or reverse osmosis and in this case a second retentate R2 which contains alkali metal salts is produced, and a second permeate P2 is produced,
  • (d) optionally the second retentate R2 is subjected to an alkaline demineralization and in this case a third retentate R3 which contains phosphate salts is produced and a third permeate P3 is produced,
  • (e) the third permeate P3 is combined with the retentate R1 in such a manner that a non-acidified quark base mix is formed, and
  • (f) the resultant mixture is subjected to a temperature treatment until denaturation occurs, and finally
  • (g) the denatured product is admixed with starter cultures and rennet and optionally
  • (h) the quark base mix thus obtained after completion of fermentation is adjusted to a defined dry matter content and protein content,
and in step (g), as starter culture
  • (i) a first mixture of five microorganisms comprising (i-1) Streptococcus thermophilus, (i-2) Leuconostoc species, (i-3) Lactococcus lactis subsp. lactis biovar diacetylactis, (i-4) Lactococcus lactis subsp. lactis and (i-5) Lactococcus lactis subsp. cremoris, and
  • (ii) a second mixture of three microorganisms comprising (ii-1) Streptococcus thermophilus, (ii-2) Lactococcus lactis subsp. lactis and (ii-3) Lactococcus lactis subsp. cremoris
  • is used.
    Owner:DMK DEUT MILCHKONTOR

    Quark base mix having enhanced taste properties

    A quark base mix is proposed having enhanced taste properties which is obtainable in that (a) raw milk is subjected to a temperature treatment and the cream is separated off, (b) the resultant skimmed milk is subjected to an ultrafiltration and in this case a first retentate R1 which contains a milk protein concentrate is produced, and a first permeate P1 is produced, (c) the first permeate P1 is subjected to a nanofiltration and / or reverse osmosis and in this case a second retentate R2 which contains alkali metal salts is produced, and a second permeate P2 is produced, (d) optionally the second retentate R2 is subjected to an alkaline demineralization and in this case a third retentate R3 which contains phosphate salts is produced and a third permeate P3 is produced, (e) the third permeate P3 is combined with the retentate R1 in such a manner that a non-acidified quark base mix is formed, and (f) the resultant mixture is subjected to a temperature treatment until denaturation occurs, and finally (g) the denatured product is admixed with starter cultures and rennet and optionally (h) the quark base mix thus obtained after completion of fermentation is adjusted to a defined dry matter content and protein content, and in step (g), as starter culture (i) a first mixture of five microorganisms comprising (i-1) Streptococcus thermophilus, (i-2) Leuconostoc species, (i-3) Lactococcus lactis subsp. lactis biovar diacetylactis, (i-4) Lactococcus lactis subsp. lactis and (i-5) Lactococcus lactis subsp. cremoris, and (ii) a second mixture of three microorganisms comprising (ii-1) Streptococcus thermophilus, (ii-2) Lactococcus lactis subsp. lactis and (ii-3) Lactococcus lactis subsp. cremoris is used.
    Owner:DMK DEUT MILCHKONTOR

    Novel milk custard and preparation method thereof

    The invention relates to a food taking powdered milk and white sugar as main ingredients, that is, novel milk custard, and a preparation method thereof. The invention is characterized in that: the provided novel milk custard contains no egg white and renninum, or glutamine aminotransferase and has rich nutrition, good taste and health care function. The novel milk custard is mainly prepared with the following raw materials by weight proportion: 35-70 parts of powdered milk, 15-50 parts of white sugar, 1.0-2.5 parts of calcium carbonate, 0.5-2.5 parts of compound carrageenan, 0.5-2.5 parts of glucomannan and 1-10 parts of other materials. Compound carrageenan and glucomannan are both gelling agents which belong to dietary fiber and have health care function; the preparation method of the invention is as follows: the raw materials are evenly blended, added with 4-5 times of warm water by weight proportion, and then heated to 80-90 DEG C, and the temperature is kept for 10-30 minutes; filling, cooling, standing still and consolidation forming are carried out to obtain the edible milk custard. The novel milk custard has the advantages of no bitter flavor, good mouthfeel, rich nutrition and health care function, and the preparation method can facilitate large-scale production and the improvement of production efficiency.
    Owner:GUANGZHOU JIANWEI BIOLOGICAL TECH CO LTD

    Quick preparation method for ultrasonic-assisted extraction of effective components of lamb abomasum

    InactiveCN106511383AShorten the timeNo reduction in biological activityDigestive systemUnknown materialsPhosphateFreeze-drying
    The invention relates to a quick preparation method for ultrasonic-assisted extraction of effective components of lamb abomasum. The method comprises the steps of adding degreased lamb abomasum powder to a phosphate buffer solution at the low temperature for dissolution, and performing ultrasonic processing; adjusting the pH value by utilizing a hydrochloric acid solution, and performing activation at the lower temperature; adjusting the pH value by using a sodium hydroxide solution, performing filtering, performing centrifugation, removing residues, and performing dialysis desalting on a supernatant solution; and performing centrifugation to remove deposits (salt-soluble proteins) formed by dialysis, and performing freeze-drying to obtain the effective components of the lamb abomasum. Through a test, the protein content of effective component extract obtained by the method reaches 71.5%, the rennin activity is 26,916 Ru/g, the pepsin activity is 3,685.2 u/g, and rennin and pepsin bands in a lauryl sodium sulfate-polyacrylamide gel electrophoresis image are clear. Compared with a conventional stirring autolysis method, the quick preparation method has the advantages that the time required for preparing the effective components of the lamb abomasum by the method can be remarkably shortened, the bio-activity is not reduced, the large-scale production is facilitated, and the method is one of ideal methods for preparing the effective components of the lamb abomasum.
    Owner:XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI

    Tannic acid type feed formula for sheep and digestion and metabolism determination method thereof

    The invention relates to the technical field of animal feed and in particular relates to a tannic acid type feed formula for sheep and a digestion and metabolism determination method thereof. Tannic acid type feed is prepared from basic feed and tannic acid, wherein the tannic acid is added into the basic feed; the adding amount of the tannic acid is 0.1g.kg<-1> to 20g.kg<-1>; the basic feed is prepared from the following ingredients in parts by weight: 35 to 40 parts of corn, 5 to 10 parts of soybean meal, 10 to 15 parts of alfalfa hay, 25 to 30 parts of corn straw, 15 to 20 parts of barley straw, 0.1 to 0.2 part of calcium hydrogen phosphate, 0.3 to 0.4 part of limestone powder, 0.5 to 1 part of table salt and 0.1 to 0.2 part of premix. After the tannic acid is added, a tannic acid protein compound is formed; the tannic acid protein compound is decomposed through pepsin and trypsin when the feed stays in a rennet stomach (with the pH (Potential of Hydrogen) of 2.5) and a small intestine (with the pH (Potential of Hydrogen) of 8 to 9), so as to form small molecular substances which are easy to absorb, and furthermore, the effect of protecting rumen bypass protein is realized. The content of the protein in milk products can be improved, so that the tannic acid can be used for improving the digestion and absorption of the feed protein in the dairy cow. A research shows that the protection effect on the feed protein is the best when the content of the tannic acid in the feed is 3.40g.kg<-1>.
    Owner:甘肃省农业科学院畜草与绿色农业研究所
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