Preparation method of alpha-lactalbumin-rich whey
A lactalbumin and whey technology, which is applied in the field of dairy product processing and preparation, can solve the problems of difficult large-scale production, lack of whey resources, and low feasibility, and achieve large-scale industrial production, low production cost, and low production cost. simple effect
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[0029] The preparation method of the whey rich in α-lactalbumin provided by the present invention may comprise the following specific steps:
[0030]Using fresh milk as raw material, preheating to 35-50°C, removing milk fat with a milk fat separator, and preparing skim milk, the fat content of which is less than 0.5% by weight;
[0031] Adjust the pH value of the skim milk to 4.8-7.0, then rapidly heat it to 70-98°C and keep it warm for about 2-25 minutes for heat treatment;
[0032] Cool the heat-treated skim milk to below 40°C; a faster cooling method than natural cooling can be used, such as circulating cooling through tap water;
[0033] Add rennet (10000-100000U / 100mL based on the volume of skim milk) and calcium chloride (0.005-0.1g / 100mL) to the cooled skim milk for enzymatic coagulation treatment to obtain casein curd and whey (that is, whey rich in α-lactalbumin), wherein the pH value of the enzymatic coagulation treatment can be controlled to the pH value of the hea...
Embodiment 1
[0038] Get fresh milk and skim it to obtain skimmed milk with a fat content of 0.15% by weight;
[0039] Adjust the pH value of the skim milk to 5.8, and heat-treat it at 85°C for 16 minutes;
[0040] Place the heat-treated skim milk in circulating tap water and cool it down to below 40°C;
[0041] Add rennet (rennet from microorganisms or plants) to the cooled skim milk in an amount of 50000U / 100mL, and add 0.02g / 100mL calcium chloride at the same time, and carry out enzymatic coagulation treatment at 42°C for 30min , the amount added is based on the volume of skim milk;
[0042] The curd and whey obtained after enzymatic coagulation were separated by centrifugation to obtain whey rich in α-lactalbumin, wherein the concentration ratio of α-lactalbumin to β-lactoglobulin was 3.32.
[0043] The obtained liquid whey can be directly used in the production of formula milk powder and the like.
Embodiment 2
[0045] Get fresh cow's milk, skim to obtain the skimmed milk that fat content is 0.03wt%;
[0046] Adjust the pH value of the skim milk to 5.2, heat treatment at 75°C for 22 minutes;
[0047] Rapidly cool the heat-treated skim milk to below 40°C;
[0048] Add rennet (rennet from microorganisms or plants) to the cooled skim milk in an amount of 30000U / 100mL, and add 0.04g / 100mL calcium chloride at the same time, and carry out enzymatic coagulation treatment at 39°C for 25min , the amount added is based on the volume of skim milk;
[0049] The curd and whey obtained after enzymatic coagulation are separated by centrifugation to obtain whey rich in α-lactalbumin, wherein the concentration ratio of α-lactalbumin to β-lactoglobulin is 2.87.
[0050] Further, by concentrating the α-lactalbumin-rich whey and then spray-drying, the α-lactalbumin-rich whey powder is obtained.
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