137 results about "Lactoglobulins" patented technology

The invention discloses a protein separation method and related application thereof, and relates to the technical field of dairy product processing, the method comprises the following steps: heating a whey solution of which the pH value is adjusted to 3.5-4.2 by a citric acid solution, carrying out microfiltration on the heated product, carrying out beta-lactoglobulin and / or alpha-lactalbumin separation on permeate and trapped fluid obtained by microfiltration, the method is based on the membrane filtration technology, high-purity separation of beta-lactoglobulin and alpha-lactalbumin is achieved, the purity can reach 70%-90% or above, the method is compatible with whey from multiple sources, equipment is simple, the method has the advantages of being low in separation cost, high in efficiency, easy to implement, large in flux and the like, and a way is provided for large-scale industrial production of beta-lactoglobulin and alpha-lactalbumin.

A method for separating protein from a milk raw material, the method comprising separating beta-lactoglobulin and alpha-lactalbumin from the milk raw material, the method comprising: a whey separation step for separating whey protein from the milk raw material; a step of ion exchange treatment: adsorbing the whey protein by using strong anion exchange resin, then eluting by using an eluent, adsorbing the whey protein by using strong anion exchange resin to obtain a flow-through liquid, enriching alpha-lactalbumin in the flow-through liquid, and carrying out gradient elution by using the eluent to obtain an elution component, at least one of the elution components is enriched with beta-lactoglobulin; and purifying the flow-through liquid to obtain an alpha-lactalbumin solid, and purifying the elution component enriched with beta-lactoglobulin to obtain a beta-lactoglobulin solid.

The invention discloses a beta-lactoglobulin fluorescent probe as well as a preparation method and application thereof, the structural formula of the probe is (C30H25N4I), the probe is combined with beta-lactoglobulin through non-covalent interaction, a fluorescence signal is triggered to be remarkably enhanced, and low-background fluorescence detection of the beta-lactoglobulin is realized. The probe has low cytotoxicity and excellent mitochondrial targeting ability, and provides a powerful tool for visualization of beta-lactoglobulin in living cells. After being combined with beta-lactoglobulin, a sample can generate obvious color change under the irradiation of an ultraviolet lamp, and the change can be used for evaluating the content of active beta-lactoglobulin in the sample, so that the rapid detection of the milk protein level in a beverage is realized. The beta-lactoglobulin fluorescent probe disclosed by the invention has a wide application prospect in the fields of biological imaging, milk protein detection, beverage quality control and the like.

The invention discloses a method for enriching whey protein main monomers from cow milk. According to the method, whey protein subjected to membrane separation is taken as a raw material, interaction between main monomers of the whey protein and other milk proteins is weakened by adopting a pH conversion technology and an ultrasonic technology, mineral substances, lactose, bovine serum albumin and residual casein are removed through ion exchange chromatography, the main monomers of the whey protein are enriched, and the high-purity whey protein is obtained. And drying the alpha-lactalbumin and the beta-lactoglobulin to obtain a milk protein product rich in two main monomers of the whey protein, and the total content of the two monomers is up to 85% or above. According to the technology, cow milk serves as a raw material, efficient enrichment of two main monomers of whey protein can be achieved only through membrane separation, pH conversion, ultrasonic assistance and ion exchange chromatography, only pH needs to be adjusted in the separation process, other chemical reagents are not added, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in the whey protein main monomer can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

The invention discloses a beta-lactoglobulin modified near-infrared fluorescent targeting probe as well as a preparation method and application thereof. The probe comprises indocyanine green (ICG) and beta-lactoglobulin (beta-LG) which are covalently bound. Compared with the prior art, the probe disclosed by the invention has the advantages that (1) the probe disclosed by the invention improves the stability of the near-infrared fluorescent dye in a body and prolongs the retention time of the near-infrared fluorescent dye, so that the observation window time is prolonged, imaging information is fully acquired in real time, and the effective circulation time in the body is prolonged; (2) the probe has the advantages of good biocompatibility, low toxicity and the like; and (3) the probe can specifically enrich in renal tubules and perform targeted imaging in kidneys, so that diagnosis and treatment of kidney injury, especially acute injury, are realized.

The invention discloses a method for extracting a target product from a milk raw material, and relates to the technical field of food processing. According to the method, before strong anion exchange chromatography is carried out, a synergistic pretreatment step of ultrafiltration and conductivity adjustment is introduced, so that acceleration, synergism, quality improvement and cost reduction of the chromatography process are realized, and the technical bottlenecks of low speed, easiness in pollution, instability and the like of the traditional ion exchange chromatography during treatment of milk raw materials are overcome; and a solution is provided for high-efficiency, high-purity and large-scale production of the beta-lactoglobulin and the alpha-lactalbumin. According to the method, strong anion exchange chromatography and weak anion exchange chromatography can be combined, so that the extraction purity of beta-lactoglobulin and alpha-lactalbumin can be further improved; in addition, the method can synchronously realize high-efficiency extraction of various target substances such as casein micelles, lactose and milk mineral salts, so that the additional value and resource utilization efficiency of milk source byproducts are greatly improved.

The invention discloses pediococcus acidilactici screening and application of pediococcus acidilactici in reducing allergenicity of whey and improving flavor, and belongs to the technical field of whey processing. The invention provides a Pediococcus acidilactici BC37, and the preservation number of the Pediococcus acidilactici BC37 is GDMCC No: 66262. The Pediococcus acidilactici BC37 can be used for preparing a Pediococcus acidilactici strain. When the pediococcus acidilactici BC37 is used for preparing the fermented whey, beta-lactoglobulin and lactose in the whey can be effectively degraded, and the degradation rates respectively reach 49.93% and 15.74%; meanwhile, after whey is fermented by the pediococcus acidilactici BC37, high-content flavor substances such as furfural, butyric acid and ethyl caprate are generated. Therefore, the pediococcus acidilactici BC37 provided by the invention has a great industrial application prospect in preparation of fermented whey products with low sensitization and unique flavor.

The present invention relates to a nutrition bar comprising a protein and extraneous β-lactoglobulin. The invention further relates to a process for the preparation of such nutrition bar. The invention further relates to the use of a β-lactoglobulin to modulate the hardness, firmness and / or cohesiveness of a nutrition bar.

The invention discloses a method for preparing low-bitterness micromolecular bovine colostrum protein peptide powder by adopting a multi-stage membrane separation and enzyme membrane coupling combined technology, and belongs to the technical field of food processing. The preparation method comprises the following steps: firstly, dividing a colostrum component into components to be hydrolyzed by adopting a multi-stage membrane separation technology, namely casein intercepted by ceramic membrane microfiltration and lactoglobulin and lactalbumin filtered by an ultrafiltration membrane, and non-hydrolytic components, namely immune globulin and lactoferrin; the preparation method comprises the following steps: firstly, separating bovine coloctrum, then deeply hydrolyzing the separated lactoglobulin, milky white and casein flocculate with the molecular weight of less than 50kDa by adopting an enzyme membrane coupling technology, mixing with unhydrolyzed immunoglobulin and lactoferrin, and freeze-drying, so that the peptide content in the treated bovine coloctrum is more than 40%, the peptide content of 200-1000Da is more than 30%, the peptide content of 200-1000Da is more than 30%, and the peptide content of 200-1000Da is more than 30%. The preparation method is simple, the preparation dispersion time is within 10 s, no obvious bitter taste exists, the retention rate of immune globulin in bovine coloctrum reaches 90% or above, and the preparation method is suitable for large-scale industrial popularization.

The invention relates to a ganoderma lucidum beverage and a making process thereof, and belongs to the technical field of drinks, the making process comprises the following steps: uniformly mixing lactobacillus acidophilus and bifidobacterium bifidum to obtain a mixed bacteria solution; inoculating the mixed bacterial liquid into a culture medium for culturing, and then mixing with a lucid ganoderma extract to obtain a lucid ganoderma active substance; packaging the lucid ganoderma active substance with maltodextrin and beta-lactoglobulin to obtain a lucid ganoderma encapsulated substance; and mixing the ganoderma lucidum encapsulation material, a sweetening agent, a thickening agent and water, homogenizing, sterilizing, cooling and aseptically filling to obtain the ganoderma lucidum beverage. According to the technical scheme, the two probiotics are mixed according to a certain volume ratio, intestinal flora balance can be effectively adjusted, proliferation of bad microorganisms in the intestinal tract can be inhibited, the probiotics and the ganoderma lucidum extract are jointly packaged in a microcapsule system formed by the maltodextrin and the beta-lactoglobulin, the stability of the microcapsule is improved, and the preparation process is simple and convenient. And the prepared ganoderma lucidum beverage has relatively good mouth feel and flavor.

The invention relates to bacillus subtilis for expressing bovine-derived beta-lactoglobulin and a construction method of the bacillus subtilis, and belongs to the technical field of microorganisms. According to the bacillus subtilis provided by the invention, bacillus subtilis BSF01 is used as a host, a constitutive promoter P566 is used for heterologous expression of a bovine-derived beta-lactoglobulin coding gene optimized by a codon, the yield of beta-lactoglobulin obtained by shake flask fermentation for 30 hours reaches 0.37 g / L, the yield of beta-lactoglobulin obtained by 3L fermentation tank fermentation for 44 hours reaches 3.06 g / L, the production intensity is 0.07 g / L / h, and the production cost is low. The bovine-derived beta-lactoglobulin is efficiently and insolubly expressed in a food safety level strain, the food industrial application of the beta-lactoglobulin is promoted, and the problems of food safety and excessive glycosylation possibly existing in heterologous expression of the beta-lactoglobulin in the prior art are solved.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The present application relates to the production of novel beta-lactoglobulin preparations and related methods, uses and food products. The present invention relates to novel methods of producing isolated beta-lactoglobulin compositions and / or compositions containing crystalline beta-lactoglobulin. The present invention also relates to novel beta-lactoglobulin compositions, uses of these compositions and food products comprising these compositions.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The invention relates to a T7RNA polymerase-guided bacillus subtilis cytosine base editor and application thereof, and belongs to the technical field of gene editing. A T7RNAP guided cytosine base editor is developed in bacillus subtilis, four kinds of cytidine deaminase from different sources are selected to be fused with T7RNAP, the cytidine deaminase is fused at the N end of the T7RNAP, and a fusion body with the highest mutation activity is obtained through screening; the cytosine base editor is used for evolving a global transcriptional regulatory factor CodY, in-situ evolution of genome protein is achieved, and the expression level of bovine-derived beta-lactoglobulin (beta-Lg) is improved. According to the cytosine base editor, the operable range of the cytosine base editor on a genome is widened, multi-site base (C-T) editing can be realized, and an editing window is large.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.

The invention discloses a fluorescent probe for beta-lactoglobulin detection and a preparation method and application thereof, and relates to the technical field of food detection. The structure of the fluorescent probe is shown in the specification, r1 and R2 are independently selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. Aggregation-induced emission (AIE) is combined with inhibition of twisted intramolecular charge transfer (TICT) when a beta-lactoglobulin (beta-LG) cavity is inserted, a D-pi-A skeleton is adopted, triphenylamine is used as a conservative electron donor and a conservative molecular rotor, and cavity coordination and static electricity are optimized by repositioning receptor connection, so that ICT and charge distribution are systematically adjusted. The cavity locking strategy integrates molecular recognition and motion limitation, and can realize rapid analysis and specific detection of beta-LG in a complex dairy product matrix.

The present disclosure describes a genetically modified microbial host cell expressing one or more heterologous genes encoding a modified beta-lactoglobulin (BLG), thereby producing the said modified BLG, wherein the modified BLG comprises at least one substitution of a non-essential and / or non-branched amino acid with an essential and / or branched amino acid compared to a parent unmodified BLG.

The invention discloses a paper-based microfluidic analysis device for beta-lactoglobulin detection as well as a preparation method and application of the paper-based microfluidic analysis device, and relates to the technical field of food detection. The paper-based microfluidic analysis device comprises a bottom plate and absorbent paper loaded on the bottom plate, the absorbent paper sequentially comprises a sample application area, an acidification area containing a buffer system with the pH range of 3-5, a deposition area overlaid with at least one layer of absorbent paper, a neutralization area containing a buffer system with the pH range of 6-8, and a detection area containing a fluorescent probe for detecting beta-lactoglobulin along the liquid flowing direction; the structure of the fluorescent probe is as follows: R1 and R2 are selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. When the paper-based microfluidic analysis device is applied, a detection sample passes through the acidification area to trigger casein precipitation, the deposition area is subjected to mechanical filtration, the neutralization area recovers the physiological pH condition before detection, and finally the detection area is combined with the fluorescent probe to generate a fluorescence detection signal.

The present invention pertains to a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for use in preventing and / or treating a metabolic disorder and / or muscle atrophy. It also pertains to a method of preventing and / or treating a metabolic disorder and / or muscle atrophy in a subject. It furthermore pertains to use of a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for increasing the level of insulin and / or glucagon in the blood of a subject and the present invention also pertains to a non-therapeutic use or method of a) beta-lactoglobulin or b) a nutritional composition comprising BLG. It also pertains to beta-lactoglobulin (BLG) for use in preventing and / or treating diabetes or prediabetes in a subject. It furthermore pertains to a nutritional composition for use in preventing and / or treating diabetes or prediabetes in a subject.

The invention provides conductive hydrogel and a preparation method thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: firstly, modifying beta-lactoglobulin by using 3-hydroxyphthalic anhydride (3-HPA), then preparing a 2-acrylamido-2-sodium methylpropanesulfonate (AMPS) precursor solution, and then adding the 3-HPA modified beta-lactoglobulin into an AMPS precursor, so as to obtain the conductive hydrogel. Firstly, beta-lactoglobulin is freeze-dried, competitive reaction of water molecules and 3-HPA is avoided, DMSO is used as a solvent when the beta-lactoglobulin is modified by 3-HPA, the problem that the solubility of 3-HPA in water is extremely low and acid anhydride is possibly hydrolyzed in advance is solved, the 3-HPA modified beta-lactoglobulin is added into an AMPS system, the conductivity of gel is enhanced, piezoelectric output is improved, and the piezoelectric effect is improved. The prepared hydrogel has high ion transmission performance and network strength, meanwhile, the material system is biocompatible, and the hydrogel can be applied to the field of health monitoring.

The present invention relates to the field of nutritional compositions, methods of providing nutritional compositions and uses thereof. More particularly, the present invention relates to a ready-to-eat powdered infant formula or ready-to-eat powdered growth formula exhibiting desirable gastric digestion characteristics. There is provided a nutritional composition comprising casein, whey protein comprising alpha-lactalbumin (aLac) and beta-lactoglobulin (bLac), and protein-coated fat droplets wherein the composition has a protein content of from 5% to 20% (w / w) based on total solids; the bLac content is at least 3% (w / w) based on the total protein level, and the aLac + bLac content is at least 25% (w / w) based on the total protein level; the fat content is in the range of 10% to 50% (w / w) based on total solids; the fat droplets are coated with an average protein load of 2 to 10 mg / m2, and wherein the weight ratio of casein: (aLacc + bLac) on the fat droplets is at least 4 times the weight ratio of casein: (aLacc + bLac) in the total composition; the level of sphingomyelin per 100 g of the composition is between 70 mg and 200 mg; the level of phospholipids per 100 g of the composition is between 280 mg and 950 mg.

The invention discloses a multi-step enzymolysis desensitization process for preparing low-sensitization beta-lactoglobulin peptide and application, and belongs to the technical field of food safety detection. According to the method disclosed by the invention, the anaphylaxis of the beta-lactoglobulin can be obviously reduced, and the antigen reduction rates are 62.46% (the combination of pepsin and papain) and 71.81% (the combination of bromelain and pepsin) respectively. The low-anaphylaxis beta-lactoglobulin peptide can effectively maintain the spleen index and the body temperature of an allergic model mouse at the physiological level, reduce the level of IgE (Immunoglobulin E) and improve the protective barrier of intestinal tracts. On the basis, the method can be effectively applied to development of food industry and functional food, meanwhile, technical support is provided for development of dairy product raw materials and products with higher safety, and wide industrial application prospects are shown.

The present invention provides a β-lactoglobulin lipoic acid hydrogel material and its preparation method and application, belonging to the field of molecular biomedical technology. Lipoic acid powder is dissolved in alkali solution, the obtained lipoic acid salt solution, β-lactoglobulin solution and carboxyl activator are mixed, pH is adjusted to carry out chemical modification reaction, and a modified product is obtained; after the modified product is dialyzed and dried in sequence, β-lactoglobulin lipoic acid powder is obtained; β-lactoglobulin lipoic acid powder is mixed with water to obtain β-lactoglobulin lipoic acid solution; β-lactoglobulin lipoic acid solution is contacted with an oil phase solution, and β-lactoglobulin lipoic acid hydrogel material is obtained after ultraviolet light irradiation. The present invention converts lipoic acid into water-soluble lipoic acid salt, increases the solubility of lipoic acid in the aqueous phase, thereby improving the efficiency of the reaction with β-lactoglobulin; adopts oil-water interface mediation, effectively exposes the photocrosslinking site, and further improves the photocrosslinking efficiency.

The present invention pertains to a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for use in preventing and / or treating a metabolic disorder and / or muscle atrophy. It also pertains to a method of preventing and / or treating a metabolic disorder and / or muscle atrophy in a subject. It furthermore pertains to use of a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for increasing the level of insulin and / or glucagon in the blood of a subject and the present invention also pertains to a non-therapeutic use or method of a) beta-lactoglobulin orb) a nutritional composition comprising BLG. It also pertains to beta-lactoglobulin (BLG) for use in preventing and / or treating diabetes or prediabetes in a subject. It furthermore pertains to a nutritional composition for use in preventing and / or treating diabetes or prediabetes in a subject.

The present invention relates to a method of preparing a modified protein composition by reaction with an oxidized phenolic compound under conditions of exposure and modification of the free thiol groups of beta-lactoglobulin. The resulting modified protein products have been found to have excellent performance in, for example, protein-rich beverage products, and have particularly been found to produce reduced levels of unpleasant odors during neutral pH sterilization heat treatments as well as during consumption of such beverage products.