81 results about "Lactoglobulins" patented technology

The invention discloses a method for extracting a target product from a milk raw material, and relates to the technical field of food processing. According to the method, before strong anion exchange chromatography is carried out, a synergistic pretreatment step of ultrafiltration and conductivity adjustment is introduced, so that acceleration, synergism, quality improvement and cost reduction of the chromatography process are realized, and the technical bottlenecks of low speed, easiness in pollution, instability and the like of the traditional ion exchange chromatography during treatment of milk raw materials are overcome; and a solution is provided for high-efficiency, high-purity and large-scale production of the beta-lactoglobulin and the alpha-lactalbumin. According to the method, strong anion exchange chromatography and weak anion exchange chromatography can be combined, so that the extraction purity of beta-lactoglobulin and alpha-lactalbumin can be further improved; in addition, the method can synchronously realize high-efficiency extraction of various target substances such as casein micelles, lactose and milk mineral salts, so that the additional value and resource utilization efficiency of milk source byproducts are greatly improved.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The present application relates to the production of novel beta-lactoglobulin preparations and related methods, uses and food products. The present invention relates to novel methods of producing isolated beta-lactoglobulin compositions and / or compositions containing crystalline beta-lactoglobulin. The present invention also relates to novel beta-lactoglobulin compositions, uses of these compositions and food products comprising these compositions.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.

The invention discloses a fluorescent probe for beta-lactoglobulin detection and a preparation method and application thereof, and relates to the technical field of food detection. The structure of the fluorescent probe is shown in the specification, r1 and R2 are independently selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. Aggregation-induced emission (AIE) is combined with inhibition of twisted intramolecular charge transfer (TICT) when a beta-lactoglobulin (beta-LG) cavity is inserted, a D-pi-A skeleton is adopted, triphenylamine is used as a conservative electron donor and a conservative molecular rotor, and cavity coordination and static electricity are optimized by repositioning receptor connection, so that ICT and charge distribution are systematically adjusted. The cavity locking strategy integrates molecular recognition and motion limitation, and can realize rapid analysis and specific detection of beta-LG in a complex dairy product matrix.

The invention discloses a paper-based microfluidic analysis device for beta-lactoglobulin detection as well as a preparation method and application of the paper-based microfluidic analysis device, and relates to the technical field of food detection. The paper-based microfluidic analysis device comprises a bottom plate and absorbent paper loaded on the bottom plate, the absorbent paper sequentially comprises a sample application area, an acidification area containing a buffer system with the pH range of 3-5, a deposition area overlaid with at least one layer of absorbent paper, a neutralization area containing a buffer system with the pH range of 6-8, and a detection area containing a fluorescent probe for detecting beta-lactoglobulin along the liquid flowing direction; the structure of the fluorescent probe is as follows: R1 and R2 are selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. When the paper-based microfluidic analysis device is applied, a detection sample passes through the acidification area to trigger casein precipitation, the deposition area is subjected to mechanical filtration, the neutralization area recovers the physiological pH condition before detection, and finally the detection area is combined with the fluorescent probe to generate a fluorescence detection signal.

The present invention pertains to a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for use in preventing and / or treating a metabolic disorder and / or muscle atrophy. It also pertains to a method of preventing and / or treating a metabolic disorder and / or muscle atrophy in a subject. It furthermore pertains to use of a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for increasing the level of insulin and / or glucagon in the blood of a subject and the present invention also pertains to a non-therapeutic use or method of a) beta-lactoglobulin or b) a nutritional composition comprising BLG. It also pertains to beta-lactoglobulin (BLG) for use in preventing and / or treating diabetes or prediabetes in a subject. It furthermore pertains to a nutritional composition for use in preventing and / or treating diabetes or prediabetes in a subject.

The invention provides conductive hydrogel and a preparation method thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: firstly, modifying beta-lactoglobulin by using 3-hydroxyphthalic anhydride (3-HPA), then preparing a 2-acrylamido-2-sodium methylpropanesulfonate (AMPS) precursor solution, and then adding the 3-HPA modified beta-lactoglobulin into an AMPS precursor, so as to obtain the conductive hydrogel. Firstly, beta-lactoglobulin is freeze-dried, competitive reaction of water molecules and 3-HPA is avoided, DMSO is used as a solvent when the beta-lactoglobulin is modified by 3-HPA, the problem that the solubility of 3-HPA in water is extremely low and acid anhydride is possibly hydrolyzed in advance is solved, the 3-HPA modified beta-lactoglobulin is added into an AMPS system, the conductivity of gel is enhanced, piezoelectric output is improved, and the piezoelectric effect is improved. The prepared hydrogel has high ion transmission performance and network strength, meanwhile, the material system is biocompatible, and the hydrogel can be applied to the field of health monitoring.

The present invention relates to the field of nutritional compositions, methods of providing nutritional compositions and uses thereof. More particularly, the present invention relates to a ready-to-eat powdered infant formula or ready-to-eat powdered growth formula exhibiting desirable gastric digestion characteristics. There is provided a nutritional composition comprising casein, whey protein comprising alpha-lactalbumin (aLac) and beta-lactoglobulin (bLac), and protein-coated fat droplets wherein the composition has a protein content of from 5% to 20% (w / w) based on total solids; the bLac content is at least 3% (w / w) based on the total protein level, and the aLac + bLac content is at least 25% (w / w) based on the total protein level; the fat content is in the range of 10% to 50% (w / w) based on total solids; the fat droplets are coated with an average protein load of 2 to 10 mg / m2, and wherein the weight ratio of casein: (aLacc + bLac) on the fat droplets is at least 4 times the weight ratio of casein: (aLacc + bLac) in the total composition; the level of sphingomyelin per 100 g of the composition is between 70 mg and 200 mg; the level of phospholipids per 100 g of the composition is between 280 mg and 950 mg.

The invention discloses a multi-step enzymolysis desensitization process for preparing low-sensitization beta-lactoglobulin peptide and application, and belongs to the technical field of food safety detection. According to the method disclosed by the invention, the anaphylaxis of the beta-lactoglobulin can be obviously reduced, and the antigen reduction rates are 62.46% (the combination of pepsin and papain) and 71.81% (the combination of bromelain and pepsin) respectively. The low-anaphylaxis beta-lactoglobulin peptide can effectively maintain the spleen index and the body temperature of an allergic model mouse at the physiological level, reduce the level of IgE (Immunoglobulin E) and improve the protective barrier of intestinal tracts. On the basis, the method can be effectively applied to development of food industry and functional food, meanwhile, technical support is provided for development of dairy product raw materials and products with higher safety, and wide industrial application prospects are shown.

The present invention pertains to a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for use in preventing and / or treating a metabolic disorder and / or muscle atrophy. It also pertains to a method of preventing and / or treating a metabolic disorder and / or muscle atrophy in a subject. It furthermore pertains to use of a) beta-lactoglobulin or b) a nutritional composition comprising beta-lactoglobulin for increasing the level of insulin and / or glucagon in the blood of a subject and the present invention also pertains to a non-therapeutic use or method of a) beta-lactoglobulin orb) a nutritional composition comprising BLG. It also pertains to beta-lactoglobulin (BLG) for use in preventing and / or treating diabetes or prediabetes in a subject. It furthermore pertains to a nutritional composition for use in preventing and / or treating diabetes or prediabetes in a subject.

The present invention relates to a method of preparing a modified protein composition by reaction with an oxidized phenolic compound under conditions of exposure and modification of the free thiol groups of beta-lactoglobulin. The resulting modified protein products have been found to have excellent performance in, for example, protein-rich beverage products, and have particularly been found to produce reduced levels of unpleasant odors during neutral pH sterilization heat treatments as well as during consumption of such beverage products.

The invention belongs to the technical field of foods, and particularly relates to a high-biological-activity muscle-building formula food for middle-aged and elderly people and a preparation method thereof. According to the high-biological-activity muscle-building formula food for the middle-aged and elderly people, the prepared mulberry leaf extract is added, the raw material raw milk is subjected to multi-stage membrane filtration to enrich beta-lactoglobulin, other auxiliary materials are adopted, muscle attenuation can be delayed, blood sugar can be remarkably reduced, sufficient nutrition support can be provided for the middle-aged and elderly people, and the high-biological-activity muscle-building formula food is suitable for the middle-aged and elderly people. The content of beta-lactoglobulin in the formula is not less than 3%, so that muscle health can be effectively improved, the immune barrier is improved, and functional decline is delayed.

This invention relates to a nutritional composition comprising a combination of β-lactoglobulin and lactoferrin. β-Lactoglobulin is a major whey protein with excellent emulsifying and gelling properties, while lactoferrin is known for its antimicrobial and immune-modulating effects. The combination of these proteins provides enhanced health benefits such as improved immune support and gut health. A key feature of this invention is the ability of β-lactoglobulin to prevent or reduce the coagulation of lactoferrin during heating, maintaining its functional integrity and efficacy. Additionally, methods for recombinantly producing lactoferrin and β-lactoglobulin, and specific formulations for targeted demographics such as children, infants, and the elderly, and formulations for healthy ageing, for improvement of women's health and / or as a sports nutrition composition are disclosed.

The invention discloses a processing method of a Wuyi rock tea drink based on a modified beta-lactoglobulin composite system, and belongs to the technical field of tea drink processing. Food-grade beta-lactoglobulin is subjected to glycosylation modification and then is compounded with xanthan gum to form the composite additive, the beta-lactoglobulin and astringent compounds such as tea polyphenol in tea soup are subjected to non-covalent binding, stable complex precipitates are formed, and the astringent taste of the tea soup is remarkably reduced; meanwhile, the modified beta-lactoglobulin and natural polysaccharides such as xanthan gum have a synergistic effect, so that the phenomena of precipitation (tea cheese) and turbidity in the storage process are effectively reduced, and the clarity of tea soup is improved to a certain extent, so that the flavor of the product is improved, and the shelf life of the product is greatly prolonged. The technological process is simple and easy to implement, and a solution with both quality and benefits is provided for the development of Wuyi rock tea drinks.

The present invention relates to a method for preparing a modified protein composition under conditions in which the free thiol groups of β-lactoglobulin are exposed to and modified by a reaction with an oxidized phenol compound. The resulting modified protein product (e.g., a protein-rich beverage product) has been found to have excellent performance, and in particular, it has been shown to reduce the level of unpleasant odors during sterilization heat treatment at a neutral pH and when such beverage products are consumed.

The application belongs to the technical field of composite materials, and particularly discloses a fiber hybrid membrane for synchronously removing radioactive cesium and strontium as well as a preparation method and application thereof. The preparation method comprises the following steps: adjusting the pH of a beta-lactoglobulin solution, centrifuging, collecting the supernatant, filtering, dialyzing, freeze-drying, and obtaining beta-lactoglobulin monomers; dissolving the beta-lactoglobulin monomers in ultrapure water, adjusting the pH, and water-bath heating to obtain an amyloid fiber solution; adding potassium titanate to the amyloid fiber solution, adjusting the pH, and obtaining amyloid fiber loaded with titanium dioxide; and mixing the amyloid fiber loaded with titanium dioxide with activated carbon to obtain an amyloid fiber hybrid membrane. The fiber hybrid membrane for synchronously removing radioactive cesium and strontium and the preparation method and application thereof have high membrane flux, excellent adsorption selectivity and cycle performance, can accurately adsorb radioactive cesium and strontium in a complex seawater system, and do not produce secondary waste in the treatment process, and are low in price.

The invention relates to the technical field of biological medicine, and discloses dual-network hydrogel for treating inflammatory bowel disease as well as a preparation method and application of the dual-network hydrogel. The preparation method of the double-network hydrogel comprises the following steps: dissolving glycyrrhizic acid into deionized water, and then adding beta-lactoglobulin to obtain a solution A; dissolving zinc chloride and 2, 3, 4-trihydroxybenzoic acid in deionized water to obtain a solution B; mixing the solution A and the solution B in proportion, and stirring for reaction to obtain the double-network hydrogel. A main framework of the double-network hydrogel is composed of a first-stage network formed by glycyrrhizic acid and zinc ions through self-crosslinking and a second-stage network formed by beta-lactoglobulin and 2, 3, 4-trihydroxybenzoic acid through Schiff base and hydrogen bond crosslinking. The invention provides the dual-crosslinked hydrogel with the skeleton being a therapeutic effective component and good plasticity and adhesion, and the dual-crosslinked hydrogel has the advantages of resistance to external severe environment and strong anti-inflammatory ability, and can effectively relieve mouse colitis.

A pharmaceutical composition comprising composite active particles that comprise β-lactoglobulin and at least one active pharmaceutical ingredient (API) is provided, with methods for producing and using same in the administration of the API to a subject. The composite active particles have a tapped density of at least about 0.35 g / cm3.

The invention discloses a preparation method of beta-lactoglobulin fibers, which is characterized by comprising the following steps: S1, placing a 10 mg / mL beta-LG solution in a room temperature environment, continuously stirring for 2 hours by adopting a magnetic stirrer, then storing at 4 DEG C, completing an overnight hydration process, and performing centrifugal operation to obtain a product A; s2, preparing a product A solution with a certain concentration, and adding the product A solution into a plasma reaction kettle; and S3, setting the input voltage U of the plasma reaction kettle, maintaining the current within 1 + / -0.002 A, and controlling the reaction temperature T and the treatment time t to obtain the finished product P-beta-LGF. The method can effectively improve the fibrosis degree of the milk protein fiber during preparation, and the prepared beta-lactoglobulin fiber has better structural characteristics and functional characteristics.

The invention discloses a plant composite antibacterial liquid composition aiming at HPV (human papillomavirus) infection and a preparation method thereof, and belongs to the technical field of biological medicines. The composition is prepared from the following components in parts by weight: 5 to 20 parts of brucea javanica extract, 3 to 15 parts of senna extract, 2 to 10 parts of anhydridized beta-lactoglobulin, 1 to 8 parts of dandelion extract, 1 to 5 parts of penetration enhancer, 0.1 to 1 part of pH (Potential of Hydrogen) regulator, 0.1 to 0.5 part of preservative and the balance of phosphate buffer solution. According to the invention, anhydridized beta-lactoglobulin is innovatively taken as a carrier, so that targeted delivery of active ingredients is realized; the anti-HPV effect is enhanced through the synergistic effect of the brucea javanica and the kalii extract; the anti-inflammatory repair function of the dandelion extract is combined, and a compound penetration enhancer and a specific preparation process are matched, so that the product has the characteristics of efficient HPV resistance, anti-inflammatory repair, mildness, no irritation and good stability.

This disclosure provides expression systems comprising secretion signals that promote production and / or secretion of proteins of interest, as well as one or more polynucleotides encoding chaperone proteins (e.g., CRT and / or PDIA3) which, as demonstrate herein, enhance production and / or secretion of proteins. Moreover, genetically modified host cells comprising these expression systems are capable of producing high levels of protein of interest, such as bovine lactoferrin (bLF), bovine lactoglobulin (bLG), or ovalbumin (Ova).

Heat stable whey protein composition comprising a protein content, based on dry matter, of 60-95 wt%, a total concentration of β-lactoglobulin, based on total protein, of at least 30 wt%, at least 90% thereof being denatured β-lactoglobulin, and a total concentration of α-lactalbumin, based on total protein, of at least 20 wt%, 60-84% thereof being denatured α-lactalbumin.

The present application relates to the field of biotechnology, in particular to a preparation method of human type III collagen.The present application first attempts to use beta-lactoglobulin as a guide protein in the construction of a human type III collagen expression system, and relies on the characteristics of the expression system to produce high-yield beta-lactoglobulin, thereby achieving high-yield human type III collagen.The present application also provides a fusion protein composed of beta-lactoglobulin as a guide protein and human type III collagen, and a method for producing human type III collagen using the fusion protein.It has been verified that the present application successfully prepares human type III collagen with natural amino acid sequences and skin care effects, and the yield is more than 15g / L, thereby achieving high-yield human type III collagen with skin care effects under the premise of ensuring safety.

The invention discloses a beta-lactoglobulin nanofiber as an adjuvant for oral delivery of therapeutic polypeptide drugs and application of the beta-lactoglobulin nanofiber, and belongs to the technical field of drug delivery adjuvants, the nanofiber is formed by heat treatment of BLG under an acidic condition, and has good gastrointestinal tract stability and severe environment tolerance; in the oral administration process, the nanofibers can temporarily induce the activity of calcium-dependent calpain to be improved, so that the intestinal epithelial permeability is enhanced, and the absorption of polypeptide drugs is facilitated; the rapid recovery of the intestinal barrier function can also be promoted. Insulin is loaded in an enteric capsule coated with 3% of sodium alginate, and by utilizing the BLG nanofiber mediated intestinal barrier regulation effect, compared with subcutaneous injection, the oral administration of insulin shows that the area under a blood glucose curve (AUC) is obviously reduced in a mouse model, and the blood glucose AUC is reduced by about 20.8%. The nanofiber is high in safety and meets the clinical large-scale production requirement.

The application discloses a beta-lactoglobulin mutant, a preparation method and application, relates to the technical field of genetic engineering, and the amino acid sequence of the beta-lactoglobulin mutant is shown as SEQ ID NO. 1; the preparation method is as follows: designing a forward primer, a reverse primer, a forward mutant primer and a reverse mutant primer; using a wild-type beta-Lg plasmid as a template, a mutant DNA segment is obtained by using overlap extension PCR technology; after the mutant DNA segment is connected with an empty vector, the obtained recombinant plasmid is transformed into an E. coli competent cell, cultured and identified; the recombinant plasmid is transformed into the E. coli competent cell, cultured and lysed; the cell supernatant is collected, purified, and the beta-lactoglobulin mutant is obtained; the application improves the binding property of the beta-lactoglobulin with specific IgE antibodies, enhances the immunological activity of the beta-lactoglobulin allergen, and enhances the sensitivity and specificity of the allergen specific IgE antibody detection result.