60 results about "Succinylation" patented technology

Soluble keratin derivatives are disclosed. The soluble keratin derivatives may include a soluble keratin protein having at least one substituted chemical group at a lysine group, terminal amine group and / or hydroxyl amino acid group of a soluble keratin protein. Soluble keratin derivatives may be formed by succinylation or quaternisation, or by reaction with fatty acid derivatives. The soluble keratin derivatives may be used in personal care formulations, and may also comprise mixtures of several different soluble keratin derivatives.

The invention relates to a preparation method for a derivative of succinylated chitosan. The preparation method comprises the following steps: dissolving water-soluble chitosan in water; adding a succinic anhydride solution in a water-soluble chitosan solution on the basis of the mole ratio of the water-soluble chitosan to the succinic anhydride of 1:(2-10) under a stirring condition; stirring and reacting for 24-60 hours under a condition of room temperature to 50 DEG C; and dialyzing, de-salting, freezing and drying the reaction liquid, thereby obtaining the derivative of the succinylated chitosan. Carboxyl groups, amino groups and a plenty of hydroxy groups are contained in a molecular structure of the derivative of the succinylated chitosan provided by the invention; the derivative has excellent water solubility and biological degradability; the biocompatibility, the physiological activity and the reaction activity of the derivative are enhanced; and the derivative has important application value in the fields, such as cosmetics, drugs, degradable biological materials, and the like.

The invention relates to the field of polymer chemistry and the field of pharmaceutical excipients. The invention particularly relates to targeted chitosan derivatives (I) or (II) containing octreotide, in particular to the N-succinyl-N-alkylate chitosan derivative and the N-alkylate carboxymethyl chitosan and a preparation method thereof, the invention further relates to an effect thereof as a solubilizer of an insoluble drug, a modification effect on micelles, liposomes and other vectors and a pharmaceutical composition which leads the derivatives to have the target function and contains the derivatives.

The invention discloses a method for preparing an antibacterial membrane by N-succinyl-chitosan immobilized lysozyme, and especially designs a natural antibacterial membrane with relatively strong water solubility. The method comprises the following steps: performing succinylation on chitosan to prepare NSC; activating the carboxyl of NSC through EDC and NHS; performing covalent binding with lysozyme; and finally, preparing the antibacterial membrane through a salivation method. The method is simple and easy to operate, and the prepared membrane has a relatively strong antibacterial property for staphylococcus aureus and listeria monocytogenes.

The invention discloses a chitosan-based pure physically-crosslinked dual-network hydrogel and a preparation method thereof. With the method provided by the invention, a double-network hydrogel crosslinked by pure physical action is constructed by using chitosan and succinylated chitosan as raw materials and an alkaline solvent system as a basis; and the obtained hydrogel is further subjected to biomimetic mineralization to improve mechanical properties. Compared with a pure chitosan hydrogel, the chitosan-based pure physically-crosslinked double-network hydrogel provided by the invention is regular and compact in structure, and is significantly improved in the mechanical properties. Meanwhile, compared with a traditional chemically-physically crosslinked chitosan-based double-network hydrogel, the chitosan-based pure physically-crosslinked dual-network hydrogel has obvious advantage in good biocompatibility. The pure physically-crosslinked double-network hydrogel prepared by using thepreparation method provided by the invention has good mechanical properties and good biocompatibility, and has good application prospect in the field of biomedicine application.

The invention relates to a method for preparing protein-based surfactant by opening protein disulfide linkage. The method is characterized by converting disulfide linkage into sulfonic group by oxidizing disulfide bond in protein of peroxide, and opening the folded coiling structure of the protein to represent the surface activity properties so as to prepare the protein-based surfactant which has the functions of emulsification, foaming, defoaming, washing, decontamination, soakage, and the like; by introducing polar group or nonpolar group into a protein side chain with opened disulfide bond, the surface activity properties of peptide fragments can be further improved or increased; moreover, by adopting treatments including acetylization, succinylation, acylation or phosphorylation, protein surface activity properties such as surface tension, emulsification, foaming, defoaming, washing, decontamination and soakage can be changed further. The method furthest prevents protein polar group and nonpolar group from being damaged, and maintains the structural integrity and property naturalness of protein molecules, thereby converting the protein into natural high-performance surfactant.

The invention relates to a method for preparing acylated casein by taking yak milk casein as a raw material. The method comprises the following steps of: (1) preparing a casein solution, namely, dissolving the milk casein in deionized water, performing magnetic stirring till the complete dissolution of the casein, regulating pH value and stabilizing the pH value at 6.5-7.5 to obtain a casein solution; (2) acylating: regulating the pH value of the casein solution to 8.0-10.0, adding solid succinyl oxide in multiple times, performing constant-temperature magnetic stirring to perform succinylation, and continuously stirring for 30-50 min after the succinyl oxide is completely added so as to realize full reaction to obtain the casein solution after acylation; and (3) obtaining an acylated casein product: adjusting the pH value of the casein solution after acylation to an isoelectric point by adopting a hydrochloric acid solution to precipitate the casein, centrifuging to remove supernatant fluid to obtain a precipitate, and performing freeze-drying on the precipitate till constant weight to finally obtain the powdered white acylated casein product. The method disclosed by the invention is easy to control and good in modification effect.

Soluble keratin derivatives are disclosed. The soluble keratin derivatives may include a soluble keratin protein having at least one substituted chemical group at a lysine group, terminal amine group and / or hydroxyl amino acid group of a soluble keratin protein. Soluble keratin derivatives may be formed by succinylation or quaternisation, or by reaction with fatty acid derivatives. The soluble keratin derivatives may be used in personal care formulations, and may also comprise mixtures of several different soluble keratin derivatives.

The invention discloses natural environment-friendly modified casein glue and a preparation process thereof and aims at providing natural environment-friendly modified casein glue prepared by taking succinic anhydride as a modified agent, casein as a raw material, starch and urea as auxiliary materials and trace monoglyceride, calcium chloride and sodium benzoate as aids and a preparation process thereof. The used modified agent succinic anhydride has small toxic and side effects, the modification effect is obvious, the carboxyl content of the succinylated casein is obviously increased, and the solubleness and other processing characteristics are obviously improved; the acylated casein is taken as a raw material to prepare an adhesive, addition of auxiliary chemicals is avoided, the environmental protection performance of the casein glue is obviously improved, and the requirements on health and environmental friendliness of the adhesive are met; the prepared casein glue is high in viscosity and low in spinnability, and the adhesion requirement on the adhesive in the industry can be met; and moreover, the modified casein glue is mild in preparation conditions, simple in preparation process and easily controlled in preparation process and is suitable for industrial production and popularization.

The present invention relates to delayed release oral formulations comprising active ingredients and modified proteins used as excipients. The proteins have chemical modifications such as succmylation, deammation, glytarylation or phosphorylation which decrease the isoelectric point of the protein compared to the protein's native isoelectric point and enhance protem-protem interactions, thereby reducing solubility and swelling, and delaying release of the active ingredient when the formulation is ingested orally. Particularly, the invention relates to tablets that comprise an excipient of chemically-modified food proteins such as soy proteins or -lactoglobulm useful for delaying release of an active ingredient such as a pharmaceutical drug or a probiotic.

The invention discloses a preparation method of modified whey protein powder, which belongs to a cow milk protein processing technology. The method comprises the following steps: (1) dissolving a whey protein into water so as to prepare a whey protein solution with a certain concentration; (2) adding a certain amount of succinic anhydride into the protein solution to carry out succinylation; (3) adjusting the pH of the protein solution subjected to succinylation to be alkaline, and then carrying out microwave treatment on the protein solution; (4) adjusting the pH of the protein solution subjected to microwave treatment to be acidic, and after the protein solution is stirred after a time, carrying out centrifugal separation on the obtained object so as to obtain a precipitation part; and (5) carrying out vacuum drying on the precipitation part so as to obtain modified whey protein powder. According to the invention, a whey protein is modified through succinylation supplemented by microwaves so as to improve the high gel property thereof, the preparation of whey protein powder with a high gel property is simple in required equipment, and safe and rapid in operation, the modified protein gel property is high, and the application of the whey protein in food is facilitated.

The invention discloses a method for synthesizing crosslinking succinyl xanthic acid chitosan and application thereof to the aspect of heavy metal ion adsorption. According to the method, a target product is obtained through the succinylation reaction, the xanthation reaction and the crosslinking reaction. The crosslinking succinyl xanthic acid chitosan obtained according to the synthesizing method has a performance of being insoluble to acid. The synthesizing method is simple and easy to operate, and the aftertreatment of the product is simple. The invention further discloses application of the crosslinking succinyl xanthic acid chitosan serving as a heavy metal ion adsorbing agent. The crosslinking succinyl xanthic acid chitosan can efficiently adsorb various metal ions in water solution, such as Pb2+, Ni2+, Cd2+ and Co2+.

Soluble keratin derivatives are disclosed. The soluble keratin derivatives may include a soluble keratin protein having at least one substituted chemical group at a lysine group, terminal amine group and / or hydroxyl amino acid group of a soluble keratin protein. Soluble keratin derivatives may be formed by succinylation or quaternisation, or by reaction with fatty acid derivatives. The soluble keratin derivatives may be used in personal care formulations, and may also comprise mixtures of several different soluble keratin derivatives.

The invention discloses a method for packing a drug compound. The method comprises the steps of synthetic succinylation of glucosan; synthetic amination of folacin analogue; synthesis of folacin-glucan conjugate; mixing of the drug compound into the folacin-glucan conjugate; and then adding of pharmaceutically acceptable auxiliary material and / or material to dry. A preparation method of an auxiliary material for packing the drug compound, produced auxiliary material, further application of the auxiliary material and the like are also disclosed by the invention.

The invention relates to a method for extracting and modifying wheat gluten proteins. The method is characterized by comprising the following steps: selecting wheat bran, degreasing, performing high-temperature alpha-amylase enzymolysis, alkali extracting, centrifuging, collecting supernatant, acid precipitating, centrifuging, collecting precipitates, drying, and modifying. Succinylation introduces succinate carboxyl with negative electricity, by improving the electronegativity, the proteins generate a higher electrostatic repulsive force, and the combination of hydrogen bonds is reduced, so that the interaction of the proteins is weakened, the interaction between the proteins and water is improved, and the solubility is further improved.

The invention discloses application of succinylation-related preparations for the regulation of mitochondrion OCR. According to the application of the succinylation-related preparations for the regulation of the mitochondrion OCR, mitochondrion oxygen consumption (p<average>is less than 0.05) is significantly reduced after treatment of human thyroid epithelial cells with palmitic acid, while the change of the mitochondrion oxygen consumption(p<average>is less than 0.05) is on the contrary after treatment of the human thyroid epithelial cells with palmitic acid and a desuccinylation inhibitor.The result of further detection of succinylation IDH2 (isocitrate dehydrogenase 2) shows that the result is achieved by regulating the succinylation of IDH2, that is, the succinylation of the IDH2 isreduced, mitochondrion OCR is reduced, and the desuccinylation inhibitor is involved in increasing or restoring succinylation of the IDH2, and the mitochondrion OCR is enabled to be increased or restored. The application of the succinylation-related preparations for the regulation of the mitochondria OCR provides a novel drug target for the treatment of congenital hypothyroidism.

The invention discloses a sustained and controlled release antibacterial rice bran protein, which composite membrane and a preparation method and an application thereof. The composite membrane is prepared through the following steps that rice bran protein is subjected to succinylation modification through succinic anhydride to obtain rice bran acylated protein, the rice bran acylated protein and carboxymethyl chitosan serve as main membrane base material raw materials, glycerin serves as a plasticizer, tween 20 is a dispersant, and curcumin-loaded rice bran nanogel is added at the same time, according to the present invention, the film base material raw materials are combined according to the specific ratio, and the raw material components achieve the synergistic effect, such that the composite film has the excellent antibacterial property and the excellent mechanical property, and the sustained and controlled release effect of the antibacterial agent in the composite film is achieved; and the composite film produced by the invention is uniform in texture and excellent in performance. As the film base material is a food-borne raw material, the film has the characteristics of edibility, environmental protection, low energy consumption and the like, is a novel food packaging film which is effective and has a wide application prospect, and has a far-reaching influence on the development of the field of food packaging.

The invention provides an intelligent pH-catalytic-response micro-nano robot. The intelligent pH-catalytic-response micro-nano robot is characterized by comprising a porous skeleton internally and totally loaded with succinylation beta-lactoglobulin and catalase, wherein the succinylation beta-lactoglobulin is beta-lactoglobulin modified by succinylation, and the porous skeleton is a ZIF-L metal organic frame. The invention provides an assembling method for the intelligent pH-catalytic-response micro-nano robot, and the assembling method includes the following steps that 1, the beta-lactoglobulin is subjected to succinylation modification to obtain the succinylation beta-lactoglobulin; 2, the succinylation beta-lactoglobulin, the catalase and a 2-methylimidazole water solution are mixed, azinc ion solution is added, the mixture continues to stir for preset time, centrifugal separation is carried out, and the intelligent pH-catalytic-response micro-nano robot is obtained. The inventionprovides application of the intelligent pH-catalytic-response micro-nano robot as a carrier to preparing tumor treatment medicine.

The invention discloses a skin surface wound repair preparation and a preparation method and application thereof. Natural high-molecular polysaccharides such as chitosan, hyaluronic acid, pullulan andthe like are used as raw materials, succinic anhydride is used as a connecting arm, 4-dimethylaminopyridine is used as a catalyst, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is takenas a dehydrating agent, a product is dialyzed to remove a catalyst and the dehydrating agent, freeze-drying is executed to obtain hyaluronic acid-based pullulan, and the hyaluronic acid-based pullulan is physically mixed with chitosan to prepare a membrane, and a skin wound repair membrane preparation is obtained; or hyaluronic acid and pullulan are linked by adopting succinylated chitosan to obtain the skin surface wound repair preparation prepared from the chemically grafted chitosan-hyaluronic acid-pullulan high molecular material. The skin surface wound repair preparation has good biocompatibility, can effectively stop bleeding, resist bacteria, absorb wound area liquid, keep wounds moist, promote epithelial cell growth and accelerate wound healing, and has the advantages of wide rawmaterial source, low cost, mild preparation conditions, simple production route and easy control of product quality.

The invention discloses a lysine succinylation modification prediction method based on bidirectional long-short term memory and a convolutional neural network. The method comprises the following steps: segmenting a succinylation protein sequence into fragments which take lysine as a center and each of which comprises 15 amino acid residues at the upstream and the downstream, and processing the balance problem of data by using a random sampling method; and sending known lysine succinylation modification data into an embedded layer of a deep learning classification model, converting amino acids into vectors for expression, then inputting the vectors into a one-dimensional convolutional layer, a pooling layer, a bidirectional long-short term memory network layer, a discarding layer, a flat layer and a full connection layer, and finally outputting information of lysine succinylation modification sites. According to the method, the semantic relationship hidden in the succinylation sequence is absorbed, and lysine succinylation modification can be quickly and effectively predicted; and the invention further develops a network prediction platform which is used for on-line prediction of lysine succinylation modification sites.

The invention discloses a novel, wide source, good biocompatibility, safe and nontoxic sustained-release preparation auxiliary material. This excipient is obtained from whey protein isolate, whey protein concentrate, or other mixtures containing one or more of the above proteins through succinylation or acetylation modification. release preparations. In the present invention, different acid anhydrides are used to acylate samples, and common drying techniques such as freeze drying, spray drying, blast drying and the like can be used to prepare auxiliary materials. The excipient enters the environment of the human body and can form a gel-like retardation layer outside the drug, thereby controlling the slow release of the drug in the human gastrointestinal tract, and is widely used in the preparation of sustained and controlled release preparations for drugs with short biological half-lives. and development.

The invention belongs to the technical field of medicine and relates to a preparation method of iron(II) casein succinylate. Casein is subjected as a material to acylation reaction with a succinylation reagent under certain conditions so as to obtain casein succinylate; the casein succinylate is complexed with a ferrite to obtain iron(II) casein succinylate. The iron(II) casein succinylate is in solid state, odorless and insoluble in water at normal temperature. The iron content in the iron(II) casein succinylate reaches 5% and above; casein succinylate and iron(II) complexing rate is not lessthan 98%; the iron(II) casein succinylate is suitable for the treatment of patients with iron-deficiency anemia and patients with iron deficiency symptoms.

The invention belongs to the technical field of medical biological materials, and particularly relates to a cold compress type medical hydrogel and a preparation method thereof. According to the cold compress type medical hydrogel, a water-soluble polymer, succinylation modified starch, collagen, a cross-linking agent and deionized water are used as a raw material system, and the starch is subjected to succinylation modification, so that the gel performance, the water absorption performance and the strength performance of the starch are further improved, the gel content, the mechanical properties such as the gel strength and the like, and the water absorption performance of the whole hydrogel system are effectively improved, the cooling effect of the hydrogel can be realized, and the hydrogel has the effect of relieving pain by further utilizing the advantage of cooling.

The invention provides a polypeptide. The polypeptide has a modification at the N-terminus compared to a polypeptide having an amino acid sequence selected from at least one of addition, methylation,formylation, carbamylation, succinylation, cyclization, propionylation, hexadecane acylation, tetradecane acylation and acetylation. The polypeptide is: (1) a polypeptide having an amino acid sequencerepresented by SEQ ID NO: 1-6; or (2) a polypeptide having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% identity as compared to (1); or (3) a polypeptide having substitution, deletion and / or addition of one or more amino acids as compared to (1). According to the polypeptide disclosed by the embodiment of the invention, the activity of the BefAprotein for promoting beta cell proliferation is maintained, the polypeptide has the characteristics of higher stability and difficulty in degradation, and the polypeptide has the potentials of reducing blood glucose and weight and protecting the liver.

The invention discloses a light-stable modified curdlan antioxidant as well as a preparation method and application thereof, and belongs to the technical field of biomass materials. According to the method, the problems of steric hindrance and water solubility of physical characteristics-reaction of carboxymethyl curdlan are considered, and the modified product is prepared by adopting EDC / DMAP esterification reaction. In the modification process of the method, carboxyl of carboxymethyl curdlan is reserved to a great extent, EGCG succinylation is carried out firstly, and then reaction with remaining hydroxyl of the carboxymethyl curdlan is carried out, so that the EGCG modified curdlan antioxidant Na-CMCUD-EGCG is prepared. According to the method disclosed by the invention, the grafting rate of the EGCG is favorably improved, the grafting rate reaches 70-150mg EGCG / g Na-CMCUD-EGCG, and the water solubility of the product is also favorably improved; and the obtained product has relatively strong antioxidant activity and light stability, and can be applied to the fields of food industry, medicine industry and daily use chemicals.

The invention discloses a pharmaceutical preparation for treating intractable epilepsy, the preparation comprises a cannabis sativa extract, a gastrodia elata extract, succinylated protein, borneol and an auxiliary agent, the active ingredient of the cannabis sativa extract is a compound with an alkyl resorcinol structure, and the active ingredient of the gastrodia elata extract is a compound with an alkyl resorcinol structure. As an extraction substance which is extracted from a cannabis sativa plant and has the same biological activity, the pharmaceutical preparation has a great synergistic effect on playing a pharmacological effect on refractory epilepsy, and the pharmacological activity of the pharmaceutical preparation is effectively improved; the gastrodia elata extract can provide effective nutrition and repair nerves in the brain, and the borneol can be used as a channel guiding drug to enhance absorption of other drugs in a blood brain barrier and effectively relieve convulsion, spasm, unconsciousness, wandering and other clinical characteristics accompanied by epilepsy disease attack, the succinylated protein in the preparation can prevent the drug from being damaged in an acid environment, and the nano-emulsion preparation prepared by adding the succinylated protein can effectively improve the bioavailability and stability of the drug.

The invention relates to a method for preparing acylated casein using yak casein as a raw material. The method comprises the following steps: (1) preparing a casein solution: dissolving casein in deionized water, stirring magnetically until the casein is completely dissolved, and adjusting The pH value is stabilized at 6.5-7.5 to obtain a casein solution; (2) acylation: adjust the pH value of the casein solution to 8.0-10.0, add solid succinic anhydride several times, and carry out succinylation with constant temperature magnetic stirring, and After all the succinic anhydride is added, continue to stir for 30 to 50 minutes to allow it to fully react to obtain the acylated casein solution; (3) acylated casein products: use hydrochloric acid solution to adjust the pH value of the acylated casein solution When reaching the isoelectric point, the casein is precipitated, and the supernatant is discarded by centrifugation to obtain a precipitate, which is freeze-dried to a constant weight to obtain a powdery white acylated casein product. The invention is easy to control and has good modification effect.

The invention belongs to the technical field of medicines and particularly provides application of glycine and a derivative thereof to preparation of a medicine for treating IDH (IsocitrateDehydrogenase) and FH (Fumarate Hydratase) gene mutation related tumors. Aiming at solving the problem that effective medicines for treating IDH gene mutation and FH gene mutation related tumors are lacked, the invention provides an effective medicine aiming at the tumors. An experiment is carried out on a mouse which is inoculated with IDH mutated cells to form the tumors under the skin, and the growth speed of the tumors can be greatly reduced by orally taking the glycine, so that the life quality of the mouse is improved, protein high succinylation in the tumors is reduced, and normal functions of mitochondria of tumor cells are enhanced. The glycine has a remarkable growth inhibition effect on the tumors and can be used for preparing the medicine for treating the related tumors.

The invention belongs to the technical field of medical biological materials, and particularly relates to an antibacterial medical cold compress hydrogel and a preparation method thereof. According to the antibacterial medical cold compress hydrogel disclosed by the invention, a water-soluble polymer, succinylated modified starch, collagen, a cross-linking agent and deionized water are taken as a raw material system, and the starch is subjected to succinylated modification so as to further improve the gel performance, the water absorption performance and the strength performance of the starch, so that the gel content, the gel strength and other mechanical properties and the water absorption performance of the whole hydrogel system are effectively improved, the cooling effect of the hydrogel can be achieved, and the advantage of cooling is further utilized, so that the hydrogel has the effect of relieving pain; and due to the addition of the antibacterial agent, the antibacterial performance of the whole hydrogel system is effectively improved, and the medical value is better.