367 results about "Mitochondrial RNA" patented technology

The present invention provides methods for rapid forensic analysis of mitochondrial DNA by amplification of a segment of mitochondrial DNA containing restriction sites, digesting the mitochondrial DNA segments with restriction enzymes, determining the molecular masses of the restriction fragments and comparing the molecular masses with the molecular masses of theoretical restriction digests of known mitochondrial DNA sequences stored in a database.

The present invention provides methods for rapid forensic analysis of mitochondrial DNA by amplification of a segment of mitochondrial DNA containing restriction sites, digesting the mitochondrial DNA segments with restriction enzymes, determining the molecular masses of the restriction fragments and comparing the molecular masses with the molecular masses of theoretical restriction digests of known mitochondrial DNA sequences stored in a database.

The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.

The present invention relates to improved diagnostic methods for early detection of a risk for developing an arthritic disorder in humans, and screening assays for therapeutic agents useful in the treatment of arthritic disorders, by comparing the levels of one or more indicators of altered mitochondrial function. Indicators of altered mitochondrial function include enzymes such as mitochondrial enzymes and ATP biosynthesis factors. Other indicators of altered mitochondrial function include mitochondrial mass, mitochondrial number and mitochondrial DNA content, cellular responses to elevated intracellular calcium and to apoptogens, and free radical production. Methods of treating, and of stratifying, human patients as such methods relate to disclosed indicators of altered mitchondrial function are also provided.

The invention relates to the technical field of traditional Chinese medicine identification, in particular to a turtle shell DNA (deoxyribonucleic acid) detection kit and an identification method. The turtle shell DNA detection kit comprises a sample pretreatment liquid, a decalcification liquid, a mitochondrial DNA extraction system, a PCR (polymerase chain reaction) system and a result observation system. The turtle shell DNA identification method comprises the steps of pretreating a detection sample, extracting mitochondrial DNA, conducting PCR primer design and synthesis, establishing PCR, and judging a result. A judgment standard is that a turtle shell is authentic if 1000bp and 500bp bands appear simultaneously, and the turtle shell is fake if only one or no band appears. The established multi-PCR identification method has the advantages of simplicity, convenience, quickness, reliable detection result and the like, and can differentiate and identify specificities of the turtle shell and the fake turtle shell accurately and simultaneously.

The present invention provides novel mitochondrial fusion transcripts, the parent mutated mtDNA molecules, and the resulting translation products (proteins) for predicting, diagnosing and / or monitoring cancer. Hybridization probes complementary thereto for use in the methods of the invention are also provided.

A novel family of human mitochondrial RNAs, referred to as chimeric RNAs, which are differentially expressed in normal, pre-cancer and cancer cells, are described. Oligonucleotides targeted to the chimeric RNAs are provided. The described oligonucleotides or their analogs can be used for cancer diagnostics and cancer therapy as well as for research. In one embodiment of this invention, these oligonucleotides hybridize with the sense or with the antisense mitochondrial chimeric RNAs, and the result of the hybridization is useful to differentiate between normal proliferating cells, pre-cancer cells and cancer cells. In another embodiment of the invention, the compositions comprise oligonucleotides that hybridize with the human chimeric RNAs resulting in cancer cell and pre-cancer cell death, while there is no effect in normal cells, constituting therefore, a novel approach for cancer therapy.

The invention discloses a multiplex-polymerase chain reaction (PCR) kit for rapidly detecting a deer product, which is used for rapidly and accurately distinguishing the deer product. The invention also discloses the preparation method of the kit. A multiplex-PCR technology is adopted to amplify the specific molecular fragments of mitochondrial DNAs of sika deer, red deer, Tarim red deer, wapiti, reindeer, pig, cow, sheep and chicken, so that the kit is prepared and used for detecting a detected sample contains the components of the animal components and detecting a mixed sample; and the kit and the method have the characteristics that is operation is simple, sensitivity is high and speed is rapid.

The invention relates to the use of active ingredients, which increase the concentration of pyrimidine-based elements for nucleic acid biosynthesis in the body, in particular to the use of pyrimidine nucleosides and / or prodrugs produced therefrom, for reducing the side-effects of inhibitors of nucleic acid biosynthesis or their precursors, in particular by activating the biosynthesis of mitochondrial DNA (mtDNA). The invention also relates to the use of said active ingredients, in particular pyrimidine nucleosides and / or prodrugs for producing pharmaceutical preparations for reducing the aforementioned side-effects and to combinations or products for administering active ingredients of this type, in particular pyrimidine nucleosides and / or prodrugs produced therefrom, comprising inhibitors of nucleic acid biosynthesis or their precursors. The invention further relates to methods for treating the side-effects of inhibitors of nucleic acid biosynthesis or their precursors using the aforementioned active ingredients, in particular pyrimidine nucleosides and / or prodrugs produced therefrom, or the aforementioned combinations or products and to corresponding pharmaceutical preparations. Side-effects of HAART (Highly Active Anti-Retroviral Therapy) and side-effects of other anti-viral nucleoside analogous agents, which inhibit the mitochondrial γ-polymerases, can in particular be prophylactically and / or therapeutically treated in this manner.