Method for preparing chymosin by mixed culture fermentation of bean dregs and waste material from fungal culture (WMFC)
A technology of mixed fermentation and rennet, which is applied to the preparation of microbial rennet, and the field of rennet prepared by mixed fermentation of bean dregs and edible fungus bran, can solve the problem that the research is limited to, does not involve the production of rennet by solid fermentation, and It involves problems such as Mucor microscopicus, and achieves the effect of high enzyme activity, simple and easy technology, and prevention of agglomeration
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Embodiment 1
[0033]The wet bean dregs (with a water content of 80-85%) are dehydrated by mechanical pressing to reduce the water content to about 65%, and then mixed with edible fungus chaff in a weight ratio of 3:1 (which has been fully washed in advance and then dried in the sun, containing 8% water), so that the moisture content of the solid fermentation medium is 50% (w / w) and the dry basis weight ratio of bean dregs and fungus chaff is about 1:1. Put the well-mixed culture medium into a triangular flask or a solid fermenter, and control the amount of material so that the thickness of the material layer is not greater than 3cm. Insert a cotton plug into the mouth of the bottle, or close the feed port of the fermenter, sterilize at 121°C for 30 minutes, and add Mucor micromyces spore suspension (concentration of 10 7 cells / mL), cultured at 35°C and 70% relative humidity for 72 hours. After the fermentation is over, take the culture material out of the fermentation container, add 10 tim...
Embodiment 2
[0036] The method for preparing chymosin by mixed fermentation of bean dregs and fungus chaff according to the present invention is characterized in that: the method specifically includes the following steps:
[0037] (1) Mechanical dehydration of bean dregs: the wet bean dregs with a water content of 80-85% are dehydrated by mechanical pressing to reduce the water content to 60%-70%;
[0038] (2) Preparation of compost: Stir and mix the dehydrated wet bean dregs: bacteria chaff = 4:1 (w:w) to make the moisture content of the solid fermentation medium 65% (w / w), and The dry basis weight ratio of bean dregs and fungus chaff is about 1:0.8; the fungus chaff has been fully washed in advance and then dried in the sun, with a water content of 7%;
[0039] (3) Filling and sterilization: put the mixed culture material into the Erlenmeyer flask, control the filling amount so that the thickness of the material layer is 0.2cm, insert a cotton plug into the mouth of the bottle, and steri...
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