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Earthworm extract having skin aging resisting effect and preparation method and application thereof

An anti-skin aging and extract technology, applied in the field of earthworm extract and its preparation, can solve the problems of heavy fishy smell of the extract, affecting product development, etc., and achieve the effects of promoting growth and proliferation, promoting synthesis, and obvious antioxidant activity.

Active Publication Date: 2014-06-11
KARLIE COSMETICS MFG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing preparation technology, on the one hand, these active ingredients are often ignored, as well as other small molecular substances such as vitamins and trace elements contained in the earthworm itself; on the other hand, the preparation of the extract has a strong fishy smell, which affects the subsequent development of the product

Method used

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  • Earthworm extract having skin aging resisting effect and preparation method and application thereof
  • Earthworm extract having skin aging resisting effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Provided is a preparation method of earthworm extract with anti-skin aging effect, comprising the steps of:

[0021] (1) Wash the fresh earthworms, put them in deionized water or pure water for 24 hours, drain the food residues in the body, and then mash the washed earthworms with a high-speed masher, and the earthworm mash liquid is the fresh earthworm sample wherein the fresh earthworms are P. aspergillum (E.Perrier)), common ringworm (P.vulgaris Chen), and William ringworm (P.guillelmi (Michaelsen)) , Pectinifera (Michaelsen) , P.hupeiensis , P.tschiliensis , P.diffringens , P.diffringens One or more of P.californica, Allolobophora caliginosa trapezoides, or Eisenia foetida.

[0022] (2) Add 2 times the volume of deionized water to the earthworm sample liquid, and use its own enzymes to hydrolyze. The enzymatic hydrolysis conditions are: pH value 6.5, temperature 40°C, hydrolysis for 3 hours, and the earthworm hydrolyzate is obtained.

[0023] (3) The earthworm hyd...

Embodiment 2

[0026] Analysis experiment of ingredients in earthworm extract:

[0027] (1) Molecular weight distribution of peptides and proteins in earthworm extracts:

[0028] Experimental procedure: According to the national standard GB / T22492-2008 method of soybean peptide powder, the molecular weight distribution of the polypeptide obtained by autolysis of earthworms was analyzed. Adopt the chromatographic column of Agilent1200 series high performance liquid chromatography, TOSOH TSK-Gel-2000SWXL (300 * 7.8mm), detection wavelength 220nm, mobile phase acetonitrile: water: the volume ratio of trifluoroacetic acid is 20: 80: 0.1, and flow velocity is 0.5ml / min, the column temperature is 25°C, and the injection volume is 20μl. A standard curve was drawn using lysozyme, insulin, bacitracin and glutathione as references.

[0029] Experimental results: see figure 1 , the HPLC test results are shown in the figure. After comparing the peak time of the standard protein, the earthworm extract...

Embodiment 3

[0039] Antioxidant activity of earthworm extract in vitro

[0040] (1) Preparation of sample solution: the earthworm extract in Example 1 was filtered with a 500 Dalton nanofiltration membrane to remove small molecular substances such as amino acids and trace elements, and an extract with a molecular weight of 500 to 30,000 Daltons was prepared as a control ; The earthworm extract without nanofiltration was used as the experimental group, and the sample solutions with different concentrations were prepared respectively.

[0041] (2) Preparation of DPPH solution: Dissolve DPPH in absolute ethanol to prepare a mother solution with a concentration of 1 mmol / L, store it in the dark at 4°C, and dilute to 100 μmol / L DPPH absolute ethanol solution before use.

[0042] (3) Take 2 mL of the sample solution and 1 mL of the DPPH solution with a concentration of 100 μmol / L, respectively, add them to the same test tube, shake well, let stand at room temperature for 30 minutes, and measure ...

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Abstract

The invention discloses an earthworm extract having a skin aging resisting effect and a preparation method and application thereof. The earthworm extract contains polypeptides of which the molecular weight is less than or equal to 30000 Dalton, amino acid, proteins, lumbricin, vitamins and trace elements. The method comprises the following steps: smashing clean earthworms or smashing dry earthworms and soaking into water to obtain earthworm sample liquid; hydrolyzing the earthworm sample liquid by using own enzyme or added protease to obtain earthworm hydrolysate; performing ultrafiltration on the earthworm hydrolysate through an ultrafiltration membrane of which the molecular weight cutoff is less than or equal to 30000 Dalton, and freeze-drying to obtain the earthworm extract. By adopting the method, original fishy smell of earthworms is removed in the obtained earthworm extract. The earthworm extract has remarkable antioxidant activity, can be used for effectively promoting the growth and proliferation of human skin fibroblasts and promoting the synthesis of substrates such as collagen to achieve the effect of resisting aging, and can be applied to development of cosmetics and health products having the effects of resisting skin aging.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an earthworm extract with anti-skin aging effect and its preparation method and application. Background technique [0002] Earthworm is a traditional traditional Chinese medicine in my country, also known as earthworm, which has the functions of clearing away heat and calming convulsions, dredging collaterals, and relieving asthma. At present, the clinical medicine of earthworms is mainly the traditional decocted extract or earthworm powder, and the crude extract of earthworm protein extracted by low temperature, which is used for thrombolysis and antithrombotic, as well as the prevention and treatment of cardiovascular, hypertension and hyperlipidemia and other diseases. [0003] Earthworms are rich in protein, fat, and active ingredients such as earthworm element, trace elements and vitamins necessary for human body. The small-molecule earthworm extract prepared by autolysis at a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/64A23L1/29A61Q19/08A23L33/00
Inventor 刘廷强周华锋侯冬梅严泽民段明星徐之伟
Owner KARLIE COSMETICS MFG CO LTD
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