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Extraction method and application for ganoderma lucidum spore active composition

A technology of Ganoderma lucidum spores and active ingredients, applied in the application of active ingredients of Ganoderma lucidum spores in anti-aging products, the field of extraction of active ingredients of Ganoderma lucidum spores, can solve the problem of large loss of nutrients, easy introduction of heavy metal elements, general ball milling efficiency, etc. problems, to achieve the effect of eliminating acne marks, shortening the time, and increasing the wall breaking rate

Inactive Publication Date: 2015-05-13
GUANGZHOU KOHEALA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ball milling efficiency of this method is average, the wall breaking rate is low, the loss of nutrients is relatively large during the ball milling process, and heavy metal elements are easily introduced

Method used

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  • Extraction method and application for ganoderma lucidum spore active composition
  • Extraction method and application for ganoderma lucidum spore active composition
  • Extraction method and application for ganoderma lucidum spore active composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for extracting active ingredients of ganoderma lucidum spores, comprising the following steps:

[0035] 1. Cultivation of Ganoderma lucidum spores

[0036] (1) The present invention adopts slant culture medium to cultivate Ganoderma lucidum strain (purchased from China General Microorganism Strain Collection and Management Center, numbered CGMCC 5.616). The slant medium is potato dextrose (PDA) agar medium, and the specific formula is shown in Table 1. The cultivation conditions of the ganoderma lucidum strains are: cultivation temperature 28° C., humidity and dark cultivation, and cultivation time is 7 days.

[0037] Table 1 Potato dextrose (PDA) agar medium formula

[0038] name

content

name

content

Magnesium Sulfate Heptahydrate

1.5g / L

Potassium dihydrogen phosphate

3.0g / L

glucose

20g / L

Vitamin B1

0.05g / L

potato extract

200g / L

[0039] (2) Primary liquid seed medium c...

Embodiment 2

[0056] A preparation method of ganoderma lucidum spore active ingredient freeze-dried powder, which comprises the following steps:

[0057] (1) Collect the ganoderma lucidum spore extract, filter it with a 0.45 μM filter membrane, and concentrate.

[0058] (2) The protein concentration in the supernatant was detected by the BCA method, and the protein concentration in the concentrate was adjusted to 50 μg / ml by a concentration method.

[0059] (3) The obtained concentrated supernatant was sterilized by filtration through a 0.22 μM membrane filter.

[0060] (4) Freeze-drying after sterilization, the freeze-drying temperature is (-20°C)-(-35°C), the vacuum degree is 50-200Pa, and the freeze-drying time is 24 hours to obtain the active ingredient freeze-dried powder of Ganoderma lucidum spores . Divide the freeze-dried powder into 1ml / bottle.

[0061] Quality standard for testing the freeze-dried powder of Ganoderma lucidum spore active ingredient

[0062] (1) The freeze-drie...

Embodiment 3

[0065] Example 3 In Vitro Toxicity Test of Ganoderma Lucidum Spore Active Component Freeze-dried Powder

[0066] (1) Using CCK-8 method (Cell Counting Kit-8) to detect the effect of the active ingredient freeze-dried powder aqueous solution of Ganoderma lucidum spores on the proliferation activity of human skin fibroblasts (HSF)

[0067] 1) Preparation of HSF cell suspension: resuspend HSF cells with 10% DMEM low-sugar medium, adjust its density to 5×104 cells / ml, inoculate them on 96-well plates at 100 μl / well, and set 4 cells in each treatment group. Each well was cultured in a 37°C incubator containing 5% CO2 for 24 hours.

[0068] 2) Experimental grouping: After 24 hours, the original culture medium was discarded, and each group was added with 10% DMEM low-sugar medium containing 1 μg / ml, 10 μg / ml, 50 μg / ml, and 100 μg / ml active ingredient freeze-dried powder of Ganoderma lucidum spores dissolved in low-sugar medium , set 10% DMEM low-glucose medium as the control group, ...

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Abstract

The invention discloses an extraction method and application for a ganoderma lucidum spore active composition. The extraction method comprises the following steps: (1) performing softening processing, namely, immersing ganoderma lucidum spore in a mixed enzyme solution containing cellulase with the mass concentration of 0.2-0.5% and pectase with the mass concentration of 0.2-0.5%, so as to soften ganoderma lucidum spore wall; (2) performing low-temperature vacuum drying; and (3) performing low-temperature wall breaking, namely, performing mechanical crushing wall breaking on ganoderma lucidum spore at a low-temperature environment. The method combines a double-enzyme solution and a low-temperature mechanical process for softening ganoderma lucidum spore cell wall, and helps to effectively reduce difficulty for crushing ganoderma lucidum spore cell wall and shorten the time. The extracted ganoderma lucidum spore active composition is free of toxic and side effects on human skin fibroblast, is applicable to anti-ageing cosmetic products, such as facial masks, face cream, emulsion and other cosmetic, and effectively reaches the effects of removing acnes, eliminating pockmark, removing wrinkles, removing fine wrinkles and the like.

Description

technical field [0001] The invention belongs to the field of microbes and cosmetics, and in particular relates to a method for extracting active components of ganoderma lucidum spores and the application of the active components of ganoderma spores in anti-aging products. Background technique [0002] Ganoderma lucidum, also known as Ganoderma lucidum grass, fairy grass, etc., is native to eastern Asia, and the most widely distributed in China is in Jiangxi. Ganoderma lucidum belongs to the fungi of Basidiomycotina, Polyporaceae, Polyporaceae, and Ganoderma genus. Li Shizhen pointed out in "Compendium of Materia Medica" that Ganoderma lucidum has the effect of "increasing wisdom and improving color". Ganoderma lucidum contains ingredients that inhibit melanin and a variety of trace elements that are beneficial to the skin. These ingredients can reduce free radicals in the human body, accelerate cell regeneration, increase skin thickness and increase collagen, so as to plump...

Claims

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Application Information

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IPC IPC(8): C12N1/06A61K8/99A61K8/02A61Q19/08A61Q19/00A61Q19/10C12R1/645
CPCC12N1/06A61Q19/00A61Q19/08A61Q19/10
Inventor 陈海佳王一飞葛啸虎罗二梅王小燕马岩岩
Owner GUANGZHOU KOHEALA BIOTECHNOLOGY CO LTD
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