The invention belongs to pore minimizing products in the field of cosmetics, and particularly relates to bio-polypeptide truly matte pore minimizing serum which comprises, in weight percentage, 10%-20% of soybean polypeptide, 10%-20% of hamamelis water, 10%-30% of aloe barbadensis leaf extracts, 5%-10% of glycine betaine, 5%-10% of dipotassium glycyrrhizinate, 1%-10% of bio-saccharide gum-1, 1%-10% of sodium hyaluronate, 1%-5% of glycerol, 1%-10% of propylene glycol, 1%-5% of methylparaben, 1%-3% of methylisothiazolinone and the rest solvents, wherein the solvents are preferably deionized water. The bio-polypeptide truly matte pore minimizing serum is capable of removing blackheads, minimizing pores, residual cutin and grease, improving skin colors, fading color spots, eliminating acne marks, diminishing inflammation, relaxing collaterals, expelling toxins, purifying, repairing and moistening skins and supplementing nutrient components lost by the skins, so that the skins are fine, smooth, white, tender, moistened, refreshed, natural and glossy, elasticity is enhanced, and health is recovered again.