The invention relates to a Chinese medicine plaster for treating diseases in muscles and bones, in particular to a plaster for promoting muscles and bones, and preparation and application thereof. The plaster is prepared by mixing pastes and powders; wherein the raw materials of the pastes by weight comprise: 25-60g of clematis root, 25-60g of heracleum, 25-60g of incised notopterygium rhizome, 25-60g of erycibe obtusifolia, 25-60g of garden balsam stem, 25-60g of gentiana macrophylla, 25-60g of prepared common monkshood daughter root, 25-60g of dried ginger, 25-60g of cinnamon, 25-60g of harlequin glorybower leaf and twig, 25-60g of rhizoma corydalis, 25-60g of ligusticum wallichii, 25-60g of frankincense, 25-60g of cowherb seed, 25-60g of lingzhi mushroom, 25-60g of gastrodia elata and 25-60g of astragalus, 25-60g of one or more of honeycomb, radix aconiti brachypodi and nux vomica, 0.1-3kg of minium and 0.5-5kg of vegetable oil; and the raw materials of the powders by weight comprise: 15-45g of clematis root, 15-45g of heracleum, 15-45g of incised notopterygium rhizome, 15-45g of phellodendron amurense, 15-45g of dragon's blood, 15-45g of gastrodia elata, 15-45g of nux vomica, 15-45g of rhizoma corydalis, 15-45g of borneol, 15-45g of harlequin glorybower leaf and twig and 15-45g of common monkshood mother root. The plaster for promoting muscles and bones has balanced effect, high medicine utilization ratio and good treatment effect.