55results about How to "Purification" patented technology

Methods to specifically detect and differentiate one or more small RNAs in miRNA maturation pathway.

A heat exchanger assembled from multiple heat exchanging tubes. Each of the multiple heat exchanging tubes is formed as a flattened tube of 0.5 mm in thickness by press work or bending work of a stainless steel plate member having a thickness of 0.1 mm. Each of the multiple heat exchanging tubes is structured to have multiple lines of sequential wave crests and multiple lines of sequential wave troughs formed on each of flattened faces of the heat exchanging tube. The multiple lines of the sequential wave crests and the multiple lines of the sequential wave troughs are arranged to have a preset angle γ relative to a main stream of an air flow. The lines of the sequential wave crests and the lines of the sequential wave troughs are symmetrically folded back about folding lines arranged at a preset interval W along the main stream of the air flow.

This invention relates to soluble forms of G glycoprotein from Hendra and Nipah virus. In particular, this invention relates to compositions comprising soluble forms of G glycoprotein from Hendra and Nipah virus and also to diagnostic and therapeutic methods using the soluble forms of G glycoprotein from Hendra and Nipah virus. Further, the invention relates to therapeutic antibodies including neutralizing antibodies, and vaccines for the prevention and treatment of infection by Hendra and Nipah viruses.

A method of purifying a polluted soil characterized in that it includes the steps of: emitting pollutants by heating soil containing the pollutants and decomposing the emitted pollutants by bringing the same into contact with functional water under light irradiation.

The invention discloses a method for sequential culture of human umbilical cord blood mesenchymal stem cells by using two culture media. A lymphocyte separation medium density gradient centrifugation method is used for separating umbilical cord blood mononuclear cells, a dulbecco modified eagle medium (DMEM)/F12 is used for primary culture of the mesenchymal stem cells of the human umbilical cord blood, and the method increases adherence of the cells, reduces growth of osteoclast like cells remarkably, facilitates formation of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) colonies, and greatly improves successful rate of culture of the hUCB-MSCs. The DMEM/F12 is used continuously for subculturing to a P2 generation, the method is combined by a using enzymatic digestion and differential velocity adherent method simultaneously, and the method facilitates purification of P1-P2 generation cells remarkably. A P3 generation and the following generations are cultured by using an Oricell human umbilical cord mesenchymal stem cell culture medium, marker proteins and good morphological characteristics and growth characteristics of the hUCB-MSCs are maintained, and multilineage differentiation potential of the hUCB-MSCs is maintained. In addition, costs of the culture media adopted by the method are reduced remarkably.

The invention relates to a biosynthesis method for improving yield of epothilone B. The biosynthesis method comprises the following steps of: selecting robust strains for producing the epothilone B by using a centrifugal process, inoculating the strains into a sterilized seed medium, performing shaking culture at 30DEG C on a shaker, and fermenting; adding a precursor inductor phenylalanine into a fermentation medium; harvesting a culture solution after culturing at 30DEG C for 10 to 11 days; and collecting, adsorbing and saturating by using macroporous resin, eluting by using ethyl acetate, separating and purifying, performing vacuum concentration, extracting, crystalizing at low temperature, and performing vacuum drying. The purity of the epothilone B can be effectively improved by a liquid preparation column, and is over 99.5 percent; and methanol is adopted for extraction and separation, so that the epothilone B is crystallized in the methanol, and the finished product reaches thepharmaceutical grade. By the method, the yield of the epothilone B is greatly improved and is 30-45 percent, and the method has a good industrial production prospect.

The invention relates to a preparation technique of beta-hydroxyalkylamide, which comprises the following steps: (a) proportionally adding diethanolamine and an alkaline catalyst into a reaction kettle, evacuating until the pressure in the kettle reaches 0.005-0.01MPa, and controlling the temperature at 50-80 DEG C; (b) dropwisely adding dimethyl adipate within 3 hours to carry out ammonolysis reaction, and evaporating to remove alcohol emitted in the reaction process; (c) after finishing dropwisely adding the dimethyl adipate, heating the reaction kettle to the high temperature of 105-120 DEG C to react for 2-4 hours, cooling to 75-95 DEG C to react for 0.5-2 hours, and keeping the viscosity of the product at 800-1000CPS; and (d) after the reaction finishes, carrying out after-treatment (crystallizing, centrifugalizing and drying) on the crude product. The mol ratio of dimethyl adipate to diethanolamine is 1:(1.8-4); the alkaline catalyst is selected from NaOH, KOH, CH3ONa and CH3ONa/NaOH; the alkaline catalyst accounts for 2-6 wt% of the dimethyl adipate; the crystallizing solvent is a mixed solvent in which the ratio of methanol to ethyl acetate is 1:(2-9); and the consumption of the crystallizing solvent is 1.5-4 times of HAA by weight. The invention can effectively enhance the purity of the product and inhibit the generation of side reactions.

The invention relates to a water purification system for artificial wetland based on oyster shell and carbon fiber. The system includes along the direction of water flow placed in sequence a water inlet grille tank, an anaerobic tank, an anoxic tank, a first grade aerobic digester, a second grade aerobic digester, an artificial wetland, a water outlet grille tank; inside the anaerobic tank and the anoxic tank are placed with oyster shell active sludge reactive barriers, inside the first grade aerobic digester and the second grade aerobic digester are placed with carbon fiber biofilm reactive barriers, the first grade aerobic digester and the second grade aerobic digester are both connected with an aeration device, the artificial wetland whose top part is planted with aquatic plants includes a sand feeding processing layer, an oyster shell feeding processing layer and a gravel feeding processing layer placed in sequence from the top to the bottom. The present invention also relates to a water purification system for artificial wetland based on oyster shell and carbon fiber. The present invention not only solves the low efficiency problem in nitrogen and phosphorus removal in sewage treatment system, but also lowers the infrastructure and operation costs of sewage treatment system to about half of the operation cost of routine biochemical treatments; it belongs to the technical field of sewage treatment.

The present invention relates to enzymes which possess hydroxylase activity, in particular 42 hydroxylase activity, that can be used in methods of synthesizing modified fatty acids.

The invention relates to a purification method of low concentration hydrogen sulfide waste gas, adds a sort of Mn (NO3)2 and Mn (CH3COO) 2 into the FeCl3 solution which contains Fe3+, evenly stirring, adds a or several kinds of ethylenediamine tetraacetic acid, sulfosalicylic acid, citric acid as the metal ion stabilizer, adjusting the solution pH value to 8-10 with Na2CO3 and NaHCO3, getting the complex ion absorption solution. Joins the complex ion absorption solution and the low thickness sulfureted hydrogen into the impact flow reactor and control the response temperature in 50-80deg.c, the oxygen content volume in the waste gas is 0.5-20%, carries on the absorption purification response to reduce the purification efficiency to lower than 70%, takes out the absorption solution and filters, the filtrate carries on the drum oxygen regeneration, the hydrogen sulfide purification efficiency may maintains above 95% in the long time. The invention is the purification method which is able to treat the hydrogen sulfide waste gas whose volume percentage concentration is below than 0.1%, with the absorption solution recycle, and high absorption purification efficiency.

The invention discloses a regeneration and purification process of a recyclable silicon material in a photovoltaic industry chain. The recyclable silicon material in the photovoltaic industry chain is subjected to regeneration and purification to produce a silicon material which satisfies solar energy-grade standard. The process includes the steps of: 1) sorting and cleaning the recyclable silicon material; 2) smelting the silicon material to remove impurities; 3) performing vacuum overflow to remove impurities; and 4) classifying products. The process is environment-friendly, simple, energy-saving and low-cost. Yield of the silicon material, which satisfies the solar energy-grade standard, is higher than 70%; and purity of the silicon material is increased from 2-3 N to more than 6 N.

The invention discloses compositions comprising soluble and membrane-anchored forms of Lassa virus (LASV) glycoprotein 1 (GP1), glycoprotein 2 (GP2), the glycoprotein precursor (GPC), the nucleocapsid protein (NP), and the nucleic acids encoding these proteins. This invention further relates to diagnostic and preventative methods using these compositions. Preventative methods include preparation of vaccines, as well as factors (e.g. small molecules) that inhibit LASV infectivity. Further, the invention relates to diagnostic and therapeutic antibodies including neutralizing antibodies for the prevention and treatment of infection by LASV and other arenaviruses.

The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.

The invention relates to a steel rail thermite welding gating system. The system comprises a sand mold, a crucible body arranged above the sand mold, a crucible cover matched with the crucible body, a shunting plug arranged inside the sand mold, and a base plate arranged at the bottom of the sand mold. According to the system, a reaction crucible and a pouring gate system are both designed to be of a liquid deslagging structure, and the purity of molten steel in a purifying cavity is fully considered. The crucible top cover of the system is in a pneumatic layout mode, reaction high-temperature gas can be dredged and high-temperature liquid-solid splash can be blocked. Two risers at the bottom of the sand mold in the system communicate with each other, and thus the casting feeding capacity is enhanced.

The invention relates to the technical field of molecular sieve preparation and application, and particularly discloses a preparation method of a stepped pore molecular sieve for refining and separating phase change wax, wherein the preparation method comprises the following steps: step 1, weighing Beta molecular sieve raw powder, carrying out ammonium ion exchange, and roasting at high temperature to remove an organic template agent to obtain an H-Beta molecular sieve; step 2, carrying out acid treatment and dealumination on the H-Beta molecular sieve obtained in the step 1, and thus obtaining a Si-Beta molecular sieve with a certain T atom defect vacancy; and step 3, taking the Si-Beta molecular sieve subjected to dealumination in the step 2 as a Si source, and carrying out secondary crystallization through an organic template agent to obtain the stepped pore Sec-SiBeta molecular sieve with high silica-alumina ratio, high mesoporous pore volume and rich Si-OH. The defects in the prior art are overcome, and the prepared molecular sieve has the advantages of micropore-mesopore composite stepped structure, regular and ordered pore structure, fewer acid sites, water resistance, thermal stability and the like.

The invention relates to a new technology for arranging loach breeding nests, and belongs to the technical field of aquaculture. According to the technical scheme, the breeding nests are manufactured through waste bicycle tyres with particular specifications in an artificial breeding pond to replace soil, and an elusive habitat is provided for ecological and healthy breeding of the loach. The new technology for arranging the loach breeding nests has the advantages that the structure is simple, the waste is turned into things of value, catching is convenient and fast, the application range is wide, the success rate is high and the management is facilitated.

Provided is a method for simultaneously breeding lobsters and crucians. The method is characterized by comprising the steps of building breeding ponds, sterilizing the breeding ponds, fertilizing water, putting fry, putting fish baits, and conducting daily management and disease control. According to the method for simultaneously breeding the lobsters and the crucians, the lobsters are bred in a shallow water area and the crucians are bred in a deep water area, so that the species, amount and size of the bred fishes can be effectively controlled, breeding costs are reduced, and the economic benefits of breeding are increased.

The invention relates to a soilless loach culturing technology, and belongs to the technical field of aquaculture. According to the technical scheme of the invention, waste bicycle tire casings of specific specifications are made into culture nests for replacing soil in an artificial culturing pond, thereby providing a hidden dwelling site for ecological and healthy culture of loaches. The soilless loach culturing technology has the advantages of simple structure, capability of turning waste into valuable, rapidness in catching, high suitability, high yield, convenience in management and the like.

The invention discloses a transposition method of an acetophenone isomer, and the transposition method of an acetophenone isomer comprises 2, 4-dichloroacetophenone synthesis and transposition reactions. According to the transposition method of an acetophenone isomer, a 2, 6-dichloroacetophenone isomer in crude 2, 4-dichloroacetophenone can be converted into 2, 4-dichloroacetophenone through a transposition reaction, so that the content of the 2, 6-dichloroacetophenone isomer in the crude 2, 4-dichloroacetophenone is reduced, the yield of the 2, 4-dichloroacetophenone is increased, the purification of the crude 2, 4-dichloroacetophenone is realized, the reaction is simple, and the transposition efficiency is high.

The invention discloses a dust and oil-gas removing apparatus, which comprises a housing, wherein at least a separator, a water supplement device and a clarification device connected with the water supplement device are arranged inside the housing, the inner wall on the upper portion of the separator is fixedly connected with the outer side of a diversion blade, a center cylinder with an induced fan is fixedly connected with the inner side of the diversion blade, the separator and the clarification device are communicated through a separator lower pipe, a stirring device is arranged inside the clarification device, and is connected with a drive device, a mud outlet position on the lower portion of the clarification device is provided with a mud outlet valve, the mud outlet is communicated with a mud pump, the upper portion of the clarification device is provided with an overflow port, the overflow port is connected with a high pressure water tank through an overflow lower pipe, a water outlet of the high pressure water tank and one high temperature main pipe end provided with a high pressure water pump are communicated, the other high temperature main pipe end provided with a high pressure nozzle is arranged on the inner side wall above a separator inlet, and a controller is connected with the drive device, the mud outlet valve and the mud pump. The dust and oil-gas removing apparatus has advantages of water resource saving and good dust recovery effect.