56results about How to "Purification" patented technology

Methods to specifically detect and differentiate one or more small RNAs in miRNA maturation pathway.

The present invention relates to enzymes which possess hydroxylase activity, in particular 42 hydroxylase activity, that can be used in methods of synthesizing modified fatty acids.

Soluble and membrane-anchored forms of Lassa virus (LASV) glycoprotein 1 (GP1), glycoprotein 2 (GP2), the glycoprotein precursor (GPC), the nucleocapsid protein (NP), and the nucleic acids encoding these proteins are disclosed, as well as diagnostic and preventative methods using these compositions. Also disclosed are methods including preparation of vaccines, factors (e.g. small molecules) that inhibit LASV infectivity, and diagnostic and therapeutic antibodies including neutralizing antibodies for the prevention and treatment of infection by LASV and other arenaviruses.

The invention provides a massaging bed; energy stones, which are capable of generating negative ions and releasing energy, are arranged in back cushions, seat cushions and foot cushions, wherein the energy stones per square centimeter can release 1600 negative ions; by virtue of the negative ions, central nervous system is regulated, so that processes of exciting and inhibiting can be normalized; electric heating wires are distributed below the energy stones; the electric heating stones, when electrified and heated, can enhance the energy release of the energy stones; magnetic stones, which are capable of releasing a magnetic field, and ultra-long wave boxes, which are capable of transmitting ultra-long waves, are additionally arranged in the back cushions and the foot cushions; the magnetic stones and the ultra-long wave boxes are arranged below the electric heating wires; the Earth is a huge magnet and human beings are impacted and affected by a geomagnetic field all the time; a magnetic field of human bodies is formed under the action of the geomagnetic field, the magnetic field is regulated by virtue of the magnetic stones, and by virtue of magnet supplementing physical therapy, an effect on preventing diseases can be achieved; and the ultra-long waves, which are transmitted by the ultra-long wave boxes, constitute an alternating magnetic field which is 50Hz in frequency, and the alternating magnetic field can enter deep layers of the human bodies and can generate a resonance energy wave with a body vibrating electromagnetic field.

The invention discloses a method for sequential culture of human umbilical cord blood mesenchymal stem cells by using two culture media. A lymphocyte separation medium density gradient centrifugation method is used for separating umbilical cord blood mononuclear cells, a dulbecco modified eagle medium (DMEM) / F12 is used for primary culture of the mesenchymal stem cells of the human umbilical cord blood, and the method increases adherence of the cells, reduces growth of osteoclast like cells remarkably, facilitates formation of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) colonies, and greatly improves successful rate of culture of the hUCB-MSCs. The DMEM / F12 is used continuously for subculturing to a P2 generation, the method is combined by a using enzymatic digestion and differential velocity adherent method simultaneously, and the method facilitates purification of P1-P2 generation cells remarkably. A P3 generation and the following generations are cultured by using an Oricell human umbilical cord mesenchymal stem cell culture medium, marker proteins and good morphological characteristics and growth characteristics of the hUCB-MSCs are maintained, and multilineage differentiation potential of the hUCB-MSCs is maintained. In addition, costs of the culture media adopted by the method are reduced remarkably.

A heat exchanger assembled from multiple heat exchanging tubes. Each of the multiple heat exchanging tubes is formed as a flattened tube of 0.5 mm in thickness by press work or bending work of a stainless steel plate member having a thickness of 0.1 mm. Each of the multiple heat exchanging tubes is structured to have multiple lines of sequential wave crests and multiple lines of sequential wave troughs formed on each of flattened faces of the heat exchanging tube. The multiple lines of the sequential wave crests and the multiple lines of the sequential wave troughs are arranged to have a preset angle γ relative to a main stream of an air flow. The lines of the sequential wave crests and the lines of the sequential wave troughs are symmetrically folded back about folding lines arranged at a preset interval W along the main stream of the air flow.

The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.