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Plant cloning technique for detoxication and fast reproduction

A technology, plant technology, applied in the field of plant reproduction by cloning, can solve the problems of poor plant growth, plant damage, premature death, etc., and achieve the effects of vigorous plant growth, huge business opportunities, and reduced pollution

Inactive Publication Date: 2002-07-03
张健
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although plant cloning technology has many advantages mentioned above, there are some technical difficulties in implementation. For example, if the cloning mother plant has pathogenic bacteria (fungus, bacteria, or virus), the cloned plants also have these pathogenic bacteria, which will cause The plants do not grow vigorously or die young; in addition, if the cloning test tube seedlings are transplanted into the soil in the wrong way, it will also cause damage to the plants or even lead to death

Method used

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Embodiment Construction

[0020] The preparation of medium is a very important part of plant cloning. There are many kinds of it currently used, but the main components are roughly the same. The main component is water, and other macroelements, trace elements, vitamins, growth regulators, sucrose and agar Wait. The invention provides a culture substrate, which has particularly good effect, and its composition is that when preparing 1 liter (1000 milliliters) of medium, add 1.65 grams of ammonium nitrate, 1.9 grams of potassium nitrate, 0.44 grams of calcium chloride, and 0.37 grams of magnesium sulfate , potassium dihydrogen phosphate 0.17 g, potassium iodide 0.83 mg, boric acid 5.2 mg, magnesium sulfate 22.3 mg, zinc sulfate 3.6 mg, sodium molybdate 0.25 mg, copper sulfate 0.025 mg, cobalt chloride 0.025 mg, iron sulfate 27.8 mg, sucrose 30 gram and 7 grams of agar, and other growth-regulating substances should be determined according to the type of plant and the purpose of cultivation. The concentrat...

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Abstract

A cloning technique for quick reproduction of plant includes preparing culture medium, choosing robust mother plant, surface sterilizing, inoculation, culturing, transplanting for naturized culture and transplanting to soil. Its advantages include high survival rate and robust plant.

Description

technical field [0001] The present invention relates to a method for breeding and breeding plants, in particular to a method for propagating plants in a cloning manner. Background technique [0002] Plant cloning is a biotechnology developed in the early 1930s. It is a method of cultivating plant organs, tissues, cells, protoplasts and other materials in a sterile state on an artificially prepared medium. Numerous small plants are regenerated from a part of the cell, or even a bare protoplast without a cell wall. Different plants have different culture substrates, and all the macroelements and trace elements are also different. At present, the number of varieties researched internationally has reached several There are hundreds of kinds. [0003] The use of cloning technology can realize the rapid propagation and promotion of excellent clones or single plants without changing their heredity, that is, keeping the original goodness unchanged. For example, one orchid stem tip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01H3/00
Inventor 张健
Owner 张健
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