Premna puberula cutting propagation method
A technology of cutting propagation and body odor firewood, which is applied in the fields of botanical equipment and methods, animal repellents, horticulture, etc., can solve the problems of lack of concentration, lack of wild resources, and small populations.
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Embodiment 1
[0017] Embodiment 1: the screening of cutting material and cutting time,
[0018] Plant material body odor wood in the present invention ( Premna puberula Pamp.) is collected from Wuchuan Gelao and Miao Autonomous County, Guizhou Province, and cultivated under three conditions: heavy shading (light transmittance 8%-10%), moderate shading (light transmittance 20%-25%) and natural light .
[0019] During the bud dormancy period (early December to mid-January), germination period (late January to late February) and leaf-expansion period (early March to late April), the annuals grown under the above three light conditions were harvested. Cuttings are carried out on strong branches without pests and diseases and without mechanical damage. The cuttings were cut to a length of 20-25 cm, the top was cut flat at a distance of 1-2 cm from the node, and the base was obliquely cut. Each cutting contained 2-3 nodes, and the survival rate was counted. Conclusion: The cuttings of C. bodh...
Embodiment 2
[0021] Embodiment 2: the screening of plant growth regulating substance,
[0022] Cut cuttings with basically the same growth conditions (the cutting method is the same as in Example 1), and place the cuttings in concentrations of 50, 100, 200, and 400 mg·L -1 The cuttings were soaked in 6-BA, KT, NAA, and IBA solutions for 2 h, and the survival rate was counted with clean water as the control. Conclusion: In 6-BA 200 mg·L -1 , 6-BA 400 mg·L -1 and NAA 200 mg·L -1 The cuttings of C. chinensis soaked in the solution have a higher survival rate of the cuttings (Table 2).
[0023]
Embodiment 3
[0024] Embodiment 3: the optimization of cutting condition,
[0025] During the dormancy period of buds, the branches of C. chinensis under moderate shading conditions were cut, and a three-factor three-level orthogonal experiment was designed with different concentrations of 6-BA, NAA and soaking time. After treatment, the cuttings were cut, and the survival rate was counted. Conclusion: The cuttings were treated with 6-BA 300 mg·L -1 +NAA 200 mg·L -1 After soaking in the solution for 1 h, the cutting survival rate was higher (Table 3).
[0026]
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