11 results about "Bud dormancy" patented technology

The present application relates to the technical field of plant gene function, and aims to provide a construction method of a homologous silencing system of a Paeonia lactiflora bud dormancy gene, comprising the following steps: designing amplification primers according to gene annotation and a conserved sequence region in a Paeonia lactiflora bud dormancy full-length transcriptome, and constructing a conserved sequence fragment of a target gene into Agrobacterium tumefaciens containing a pTRV vector; taking a rhizome of a treated Paeonia lactiflora annual seedling as an infection material, and placing the infection material in Agrobacterium tumefaciens liquid containing pTRV1, pTRV2 and pTRV2-target gene fragments to perform infection treatment; potting the rhizome and moving the rhizome into a culture room to perform culture, observing phenotype and expression amount differences between the rhizome and a control group in a growth process, and confirming that the homologous silencing system is successfully constructed. The present application first completes miniaturization treatment and batch bacterial liquid infection of an underground rhizome system of Paeonia lactiflora, establishes a virus-mediated homologous function silencing system of a Paeonia lactiflora bud dormancy gene, and realizes the purpose of verifying functions of bud dormancy and even germination and growth related genes.

The invention discloses a Chinese orchid bud dormancy control gene CsABF2 as well as a coding sequence and application thereof, and belongs to the field of plant molecular biology. The invention finds that the expression of CsABF2 in Chinese cymbidium stems is the highest; in different flower development stages of cymbidium sinense, the expression trend of CsABF2 is that the expression quantity is reduced after S1 and is gradually increased after S2; in tissue parts of mature flowers of cymbidium sinense, CsABF2 has the highest expression in labial flaps. After the CsABF2 is subjected to heterologous expression in arabidopsis thaliana, the arabidopsis thaliana is found to have a phenotype of flowering delay. After CsABF2 is silenced in Chinese orchid, the phenotype of flowering in advance can appear. The Chinese orchid flowering regulation and control gene can be used for research of orchid flower differentiation molecular mechanism and improvement of plant flowering traits.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a PsRBL gene for promoting dormancy breaking of peony buds and application of the PsRBL gene. The nucleotide sequence of the PsRBL gene is as shown in SEQ ID NO. 1. In-depth study proves that overexpression of the PsRBL gene in peony can effectively promote release of a bud dormancy state and promote germination and growth of buds; and when the gene is silenced, the release of bud dormancy can be inhibited. The discovery clearly reveals that the PsRBL gene plays an important role in the peony bud dormancy regulation and control process through H3K4me3 apparent modification. The invention lays a solid technical foundation for improving the dormancy habit of the peony buds and cultivating novel peony varieties through a genetic engineering means.

This invention discloses a gene for controlling the dormancy of Cymbidium orchid buds. CsABF2 Its coding sequence and applications belong to the field of plant molecular biology. This invention discovers that... CsABF2 The highest expression was observed in the stems of Cymbidium goeringii; it was also observed at different flower development stages in Cymbidium goeringii. CsABF2 The expression trend was that the expression level decreased after S1 and gradually increased after S2; in the tissue parts of mature flowers of Cymbidium goeringii, CsABF2 It is expressed most highly in the lip. Heterologous expression in Arabidopsis thaliana. CsABF2 Later, it was discovered that Arabidopsis thaliana exhibited a delayed flowering phenotype. (Silent in Cymbidium orchids) CsABF2 CsABF2 Subsequently, an earlier flowering phenotype may appear. The flowering regulatory gene of Cymbidium goeringii of this invention can be used to study the molecular mechanisms of flower differentiation in orchids and to improve the flowering traits of plants.

Disclosed is a pesticide having a polysulphane as active agent, and being either synthetically prepared or derived from garlic oil. The active is held as a disperse oil phase within an aqueous continuous phase. A surfactant, typically comprising a carbohydrate fatty acid ester is employed to retain the two-phase system. The diameter of the emulsion droplets, and / or the surface area of the emulsion system, is controlled to optimise the effectiveness of the pesticide contained therein. Also disclosed is a granule having an inert carrier matrix such as a diatomaceous earth or a cellulosic material, for example wood cellulose, in which the poly sulphane-containing emulsion is supported. The use of emulsion containing granules in soils to release pesticide in a controlled manner into soil is also disclosed. Also disclosed is the use of the emulsion system for accelerating breaking of bud dormancy.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a PsASH2R gene for promoting dormancy breaking of peony buds and application of the PsASH2R gene. The nucleotide sequence of the PsASH2R gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). In-depth study proves that the dormant state of buds can be effectively relieved by overexpressing the PsASH2R gene in the peony, and germination and growth of the buds are promoted; and when the gene is silenced, the release of bud dormancy can be inhibited. The discovery clearly reveals that the PsASH2R gene plays an important role in the peony bud dormancy regulation and control process through H3K4me3 apparent modification. The invention lays a solid technical foundation for improving the dormancy habit of the peony buds and cultivating novel peony varieties through a genetic engineering means.

Biostimulant compositions for bud growth in fruit trees comprising bacteria capable of producing HCN, and processes for stimulating bud growth of fruit trees in vivo are described.

The invention provides an application of a PaBBX30 protein and a coding gene thereof in regulating dormancy delay of a plant terminal bud, and belongs to the technical field of plant genetic engineering. The amino acid sequence of the protein PaBBX30 is as shown in SEQ ID No. 2. The method comprises the following steps: connecting a gene for coding the PaBBX30 protein to a p2301s vector by a homologous recombination method to construct an over-expression vector; the overexpression vector is introduced into a plant by adopting an agrobacterium-mediated transformation method, a positive transgenic line is screened out through kanamycin, and whether the PaBBX30 gene in the transgenic line is expressed or not is detected; the overexpression strain is subjected to short-day treatment, the dormancy performance of the overexpression strain is observed, and the terminal bud dormancy of the overexpression PaBBX30 gene strain is obviously delayed. The method is suitable for being applied to growth rhythm regulation and breeding improvement technologies of landscaping plants, fruit trees or other perennial plants.

The invention belongs to the technical field of plant biology, and particularly relates to a method for breaking dormancy of winter buds of kiwi fruits and promoting germination. The method comprises the following steps: S1, collecting annual kiwi fruit bearing branches meeting a preset cold demand condition after the field air temperature is lower than 7 DEG C for continuous 3 days; s2, carrying out surface disinfection treatment on the collected fruiting branches; s3, smearing or spraying a dormancy breaking agent on bud points on the fruiting branches treated in the step S2; and S4, placing the base parts of the bearing branches treated in the step S3 under a water culture condition, and culturing under the conditions that the temperature is 20-25 DEG C and the light-dark period is 12 hours until winter buds germinate. According to the method for breaking dormancy of winter buds of kiwi fruits and promoting germination, the period is remarkably shortened; according to the method disclosed by the invention, branch collection meeting partial cold demand is combined with efficient dormancy breaking agent treatment, and an optimal dormancy breaking starting condition is created.

The invention relates to application of a PaBBX28 / 29-2 protein and a coding gene thereof in promoting plant dormancy breaking, and belongs to the technical field of plant genetic engineering. The amino acid sequence of the protein PaBBX28 / 29-2 is as shown in SEQ ID No. 2. Experiments prove that the PaBBX28 / 29-2 gene can promote plant dormancy release by up-regulating expression of a bud dormancy key gene PdeuFT1 for the first time, and the action mechanism of the PaBBX28 / 29-2 gene as a positive regulation factor is disclosed. The invention also provides an amino acid sequence of the PaBBX28 / 29-2 protein, a nucleic acid sequence of a gene for coding the PaBBX28 / 29-2 protein, a functional primer, an over-expression vector and a genetically engineered bacterium. The PaBBX28 / 29-2 protein can be widely applied to growth rhythm regulation and molecular improvement of garden plants, forest trees and other perennial plants.

The application discloses a shaping method of a 'Su Cui No.1' pear tree fork-shaped tree shape, which only retains 3-5 fruiting branches per plant, is distributed in a 45-degree fork shape, has a vertical interval of 20 cm between adjacent branches, forms a 'layered ventilation zone', breaks through a traditional 'central trunk-main branch-fruiting branch' grading mode, constructs a 'trunk-fruiting branch' two-level simplified structure, reduces management levels from the root, dynamically adjusts a base angle of the fruiting branch to slightly form a funnel-shaped crown layer which is narrow at the bottom and wide at the top, controls the tree height to be less than or equal to 2.3 m, and 80% of the fruiting branches are distributed in a 0.8-1.8 m golden operation zone. The application solves the comprehensive problems of the 'Su Cui No.1' pear tree, such as frequent bud dormancy, serious early leaf fall and labor-consuming cultivation management, through a tree structure innovation and a dynamic management system, realizes early fruiting, high yield, stable yield and quality improvement of the pear tree, and is suitable for labor-saving cultivation of a large-scale pear orchard.