31 results about "Gentiana straminea" patented technology

The invention discloses a gentiana straminea beta-amyrin synthase gene GsAS2 and yeast and a plant expression vector containing the gene GsAS2. Meanwhile, the invention also discloses an application of the gentiana straminea beta-amyrin synthase gene GsAS2 and the plant expression vector containing the gene GsAS2 to preparing oleanolic acid. The inventon provides theoretical basis and practical foundation for increasing the content of target product oleanolic acid.

The invention relates to a novel secoiridoid compound and application in the preparation of anti-inflammatory drugs. A novel compound is extracted and separated from gentiana straminea, has a chemical structural general formula shown in a formula I and has anti-inflammatory activity. The invention also relates to a use of the compound or pharmaceutically acceptable salts of the compound in the preparation of drugs, in particular to a use in the preparation of anti-inflammatory drugs. The invention also relates to a pharmaceutical composition taking the compound as a main component.

The invention relates to the technical field of traditional Chinese medicinal material quality control and identification, in particular to a near infrared spectrum detection method for evaluating thequality of a medicinal material of gentiana straminea maxim. The method comprises the following steps of: processing a gentiana straminea maxim medicinal material sample and acquiring and processingnear infrared spectrum data; extracting gentiopicroside and loganic acid from the sample for content determination by means of high performance liquid chromatography, importing data and correspondingnear infrared spectrum data into software TQ Analysis, and establishing a model by taking the total amount of gentiopicroside and loganic acid as an evaluation index, and carrying out evaluation. Themethod can be used for simply, conveniently, quickly, accurately and efficiently detecting the quality of the medicinal material; when the sample points are arranged, a resource distribution area is wide in coverage, the sample size is large, and the collected samples are systematic, representative and different; related data of a target absorption peak can be rapidly extracted by adopting the infrared spectrum data extraction software, so that 2/3 of data extraction time is saved; and the method can ensure the most original information of the sample and reflect the interaction and overall relationship of the chemical components of the medicinal material.

The invention relates to a method for preparing gentiopicroside chemical reference substances from gentiana straminea maxim. The method includes the following steps: (1), drying gentiana straminea maxim herbs in the shade, smashing the herbs, and performing alcohol extraction, filtration and drying on the smashed herbs to obtain paste, namely gentiana straminea maxim extracts; (2), dissolving the gentiana straminea maxim extracts in methanol solutions before resin column separation, eluting and removing high-polarity impurities by water solutions, performing step gradient elution by 30% of methanol solutions and 50% of methanol solutions, collecting 50% of eluates, and drying the eluates under reduced pressure to obtaine gentiana straminea maxim crude products; (3), dissolving the gentiana straminea maxim crude products in the methanol solutions, collecting main chromatographic peak cut fractions in preparative chromatograms through filtering and inverted liquid phase preparative chromatography separation, and drying the chromatographic peak cut fractions under reduced pressure to obtain the white powdery reference substances with purity larger than 98%. The method has the advantages of short separation time, good separation effect and high product purity.

The invention discloses a twisted Gentiana beta-amyrin synthetase gene GsAS1 and yeast and plant expression vector containing the gene GsAS1, also discloses a twisted Gentiana beta-amyrin synthetase gene GsAS1 and the applications of the plant expression vector containing the gene GsAS1 in the preparation of the oleanolic acid. The invention provides a theoretical basis and practical basis for improving the oleanolic acid content in the target products.

The invention relates to a judgment method for quickly distinguishing two similar medicinal materials. The method comprises the following steps: (1), precisely absorbing swertiamarin standard product solution with different volumes, analyzing by using a high performance liquid chromatograph (HPLC), drawing a standard curve and calculating a regression equation; (2), precisely absorbing gentiopicroside standard product solution with different volumes, analyzing by using the HPLC, drawing a standard curve and calculating a regression equation; (3), verifying the regression equations of the swertiamarin and the gentiopicroside respectively; (4), collecting swertia pseudochinensis hara samples; (5), testing the swertia pseudochinensis hara samples to obtain the swertiamarin content and the gentiopicroside content of the swertia pseudochinensis hara samples respectively; and (6), when the swertiamarin content is less than 1 percent and the gentiopicroside content is more than 1 percent, the swertia pseudochinensis hara samples are Swertia mussotii Franch; and when the swertiamarin content is more than 2 percent and the gentiopicroside content is less than 1 percent, the swertia pseudochinensis hara samples are Swertia franchetiana. The method is simple, convenient and quick, and can guarantee the medication correctness.

The invention discloses a novel application of a gentiana straminea maxim alcohol extract to anti-anoxia. Through observing the survival time of mice in various anoxia models such as normal-pressure anoxia, sodium nitrite toxic chemical anoxia, myocardial anoxia and acute cerebral ischemic anoxia, the hydrogen peroxide (CAT) content, the superoxide dismutase (SOD) content, the malondialdehyde (MDA) content and the total antioxidant capacity (T-AOC) of the liver of the normal-pressure anoxic mice are measured to research the anti-anoxia function of the gentiana straminea maxim alcohol extract. The result shows that the gentiana straminea maxim alcohol extract has the functions of remarkably prolonging the survival time of the mice under various anoxia conditions and improving the liver antioxidant enzyme activity of the normal-pressure anoxic mice so as to protect the anoxia-induced liver oxidative stress injury, and the result explains that the gentiana straminea maxim alcohol extract has an anti-anoxia function. The gentiana straminea maxim alcohol extract has a novel application to the preparation of anti-anoxia drugs and has a favorable clinic application prospect.

The invention discloses a tissue-culture rapid propagation method for Gentiana arethusae. The method comprises the following steps: (S1) extracting root blocks; (S2) cleaning, disinfecting and sterilizing the root blocks; (S3) transplanting the root blocks into a primary culture medium; (S4) selecting plants with adventitious buds with the lengths of 3cm-6cm, taking out the plants, and cutting root blocks, wherein each cut root block is provided with 2-3 adventitious buds; (S5) transplanting each cut root block into a proliferation culture medium, and carrying out culturing; (S6) carrying outtwo-step cutting on 7-9 plants with the adventitious bus with the length of over 5cm on the same root block: firstly cutting branches with the bud length of over 5cm, and secondly cutting the root blocks; (S7) taking out the plants, singly separating buds with the stem thickness of 1.5mm-2.0mm together with base rhizome tissues, and planting the separated plants into a rooting culture medium, andcarrying out culturing; and (S8) carrying out rooting culture for 25-35 days, integrally taking out the rooting plants, transplanting the rooting plants into a seedling hardening bag, carrying out seedling hardening, and transplanting the rooting plants into a culture container.

The invention provides a thin layer identification method of alpine gentian herb and various varieties; the method includes the following operation steps: (1) taking a to-be-detected medicinal material, extracting with ethanol or methanol, and preparing a test sample solution; (2) taking the test sample solution, dotting the sample on a thin layer plate, carrying out thin-layer chromatography with ethyl formate-methanol-water-formic acid with the ratio of 10:1:1:0.5 as a developing solvent, after completion of chromatography, placing the thin layer plate under an ultraviolet lamp, and/or after color developing, carrying out visual inspection. The identification method can distinguish a plurality of different-original varieties of alpine gentian herb from confusion or counterfeit species, and provides a guarantee for the quality of the alpine gentian herb medicinal material.

The invention belongs to the technical field of planting of Chinese medicinal materials, in particular to a sowing and planting method for improving the medicinal value of Gentiana scabra. The specific method is as follows: (1) in mid-March, immersing seeds of Gentiana scabra a nutrient solution of magnesium chloride, taking out the seeds from a filtrate, conducting electric field treatment, drying the seeds to have a moisture content of 24% to 26% for sowing; (2) treating planting land before sowing, applying decomposed folium-mori cattle manure, conducting shallow ploughing to have a depth of 18-20 cm, conducting bedding and building a shed; and (3) starting to spray a nutrient solution to leaf surfaces 24 to 28 days after seedling emergence, then spraying a nutrient solution to leaf surfaces once every 85 to 90 days until harvest is started; and from mid-May to mid-September every year, conducting 80% shading treatment from 8:00 to 17:00 every day, and conducting harvesting at the end of October of a second year. Therefore, the emergence rate reaches 98% or more, breeding of harmful bacteria is effectively inhibited, and harvest can be carried out in the second year, and the gentiana scabra gentiin content, erythricine content and gentiobiose/gentianose content are remarkably improved.

The invention relates to a Tetracera asiatica lung-clearing and phlegm-eliminating dry vegetable and a preparation method thereof. The dry vegetable comprises the following raw materials by weight: 50-60 parts of bamboo shoots, 20-30 parts of Gynura bicolor, 20-30 parts of Artemisia selengensis, 20-30 parts of spinach, 20-30 parts of Meicai, 10-15 parts of Tetracera asiatica, 5-10 parts of fewflower lysionotus herb, 5-10 parts of rohdea japonica, 3-5 parts of Semiliquidambar cathayensis, 3-5 parts of Gentiana scabra, 3-5 parts of Piper sarmentosum, 1-3 parts of evodia lepta, 1-3 parts of Chinese Elder Herb, 1-3 parts of dry dendrobium, 10-15 parts of soy sauce, 5-8 parts of liquor, 5-8 parts of mature vinegar, 5-8 parts of white granulated sugar, and 3-5 parts of edible salt. The preparation method includes the specific steps of: 1, washing and soaking; 2, crushing, mixing, decoction and extraction; 3, decoction, soaking and salting; and 4, drying, sterilization and bagging. After long-term eating, the Tetracera asiatica lung-clearing and phlegm-eliminating dry vegetable can reach the effects of clearing lung and eliminating phlegm, clearing away heat and toxic materials, strengthening heart and promoting diuresis.

The invention relates to a preparation method of an effective part of gentiana straminea with inflammation-diminishing and pain-relieving functions and a medicine, and belongs to the technical field of separation of effective parts of traditional Chinese medicine. The preparation method is characterized by comprising the following preparation steps of collecting the ground part of the gentiana straminea in the flowering stage, putting at the shady and air-ventilating area, and drying; crushing the dried ground part of the gentiana straminea to obtain the crushed material, adding water into thecrushed material, and extracting by a heat reflux method, wherein the extracting temperature is 67 to 72 DEG C, and the mass ratio of the crushed material to the water is 1:(11 to 15); adsorbing andseparating the filtrate after filtering of the extracted solution by macroporous resin, and desorbing from the macroporous resin, so as to obtain the effective part; concentrating the effective part into cream, and mixing with soluble starch and sugar powder, so as to obtain particles. The preparation method has the advantages that the cost is low, and the residue of organic solvent is avoided; the effective part with higher content of effective components is extracted at higher extracting rate, and the prepared particles are uniform and are easy to dissolve.

The invention discloses a method for removing gentiana straminea polysaccharide pigment by using a three-dimensional porous amino graphene decolorizing column. According to the method, three-dimensional porous amino graphene hydrogel is loaded into a chromatographic column to prepare the decolorizing column, then the decolorizing column is applied to the field of gentiana straminea polysaccharide pigment removal, the application and removal process is simple, and the method is economical, and the cost is appropriate; the prepared three-dimensional porous amino graphene decolorizing column is successfully applied to decolorization of various polysaccharides, the decolorization rate and the recovery rate are very high, the decolorization efficiency in the removal process is high, and the polysaccharide retention rate is high. Besides, the three-dimensional porous amino graphene decolorizing column provides superior practical functions, exceeds many traditional methods, and overcomes the problems of use of organic reagents, difficulty in reuse and the like. Tests prove that when the gentiana straminea polysaccharide is decolored by the method, the decoloring efficiency is more than 95%, and the polysaccharide retention rate is more than 95%.

The invention discloses a high-yield cultivation method of Gentiana straminea Maxim in Dongyuan of the Qinghai-Tibet Plateau. The method comprises the following steps of: soaking seeds in warm water and treating the seeds in a moist environment at first, and then uniformly spreading mixture of palygorskite having a specification of 100-140 meshes, nitrogenous fertilizer, phosphatic fertilizer and potassic fertilizer in a ratio being 3000: 250: 150: 50 (kilograms per hectare) on the surface of soil; applying 30 tons of farmyard manure per hectare, and then deeply turning up the soil, finely harrowing and flatly grinding for sowing; sowing the treated seeds in the prepared soil and culturing young seedlings in May of the first year; and transplanting the young seedlings by a film-side furrow planting method from the end of April of the second year to the beginning of May of the next year, covering by black mulching films during transplanting, and performing proper field management steps, so as to obtain high-quality and high-yield Gentiana straminea Maxim.