Method for testing influence of dual stress on physiological characteristics of growth of gentiana straminea

A technology of Gentiana chinensis and physiological characteristics, which is applied in the measurement of color/spectral characteristics, material analysis through observation of the influence of chemical indicators, and testing of plants/trees, etc. It can solve the problems of low germination rate and seedling survival rate, and achieve guarantee Authenticity, yield and quality improvements

Inactive Publication Date: 2021-07-20
SHAANXI INST OF INT TRADE & COMMERCE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In production, Gentiana mahua is often caused by drought and saline-alkali stress, resulting in low germination rate and seedling survival rate, that is, in high-altitude areas, the dual stress of drought and saline-alkali has a huge impact on the normal growth of Gentiana mahua

Method used

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  • Method for testing influence of dual stress on physiological characteristics of growth of gentiana straminea

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Using NaCl+Na 2 CO 3 Different stress conditions were simulated from PEG-6000. The control group in this experiment was distilled water. The concentration of PEG-6000 was set at 2%, 4%, 6%, 8%, 10%, and 12%, and the concentration of saline-alkali stress was 10+10 .

[0061]

[0062] Table I

Embodiment 2

[0064] A test method for the influence of double stress on the physiological characteristics of the growth of Gentiana quinata, including the combination of N1PI, the concentration of saline-alkali stress 5+10mmol / L, and the PEG-6000 concentration of 2% combined 10ml solution, placed in a petri dish, Then put the petri dish into an artificial climate box for cultivation (60% relative humidity, 16h / d, 25°C), and replace the petri dish every 2 days. The number of seeds germinated was counted daily, and the radicle length > 1mm was taken as the standard, and the statistics were continued for 8 days. The seed germination rate on the 6th day was taken as the final seed germination rate, and the seed germination rate, germination potential, and germination index were calculated in turn, and the stem length, root length, fresh weight and related physiological indicators of the seedlings were measured after the 8th day.

Embodiment 3

[0066] A test method for the influence of double stress on the physiological characteristics of the growth of Gentiana gentiana, including the combination of N3P4, the concentration of saline-alkali stress 25+25mmol / L, and the PEG-6000 concentration of 8% combined 10ml solution, placed in a petri dish, Then put the petri dish into an artificial climate box for cultivation (relative humidity 60%, 16h / d, 25°C), and replace the petri dish every 2 days. Count the number of seeds germinated every day, taking the radicle length > 1mm as the standard, and continue counting for 8 days. The seed germination rate on the 6th day was taken as the final seed germination rate, and the seed germination rate, germination potential, and germination index were calculated in turn, and the stem length, root length, fresh weight and related physiological indicators of the seedlings were measured after the 8th day.

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Abstract

The invention provides a method for testing the influence of dual stress on physiological characteristics of growth of gentiana straminea, and relates to the field of planting of gentiana straminea. The method includes the following test projects that the stress concentration is set, and NaCl + Na2CO3 and PEG-6000 are adopted for simulating different stress conditions; the physiological indexes of gentiana straminea in the seed germination stage and the seedling stage in the culture under different stress conditions are detected; Gentiana straminea seedlings cultured under different stress conditions are transplanted into a field, related physiological indexes are detected, a theoretical basis is provided for research and standardized cultivation of drought resistance and saline-alkaline resistance mechanisms of gentiana straminea in a germination stage and a seedling stage, and a technical guarantee is provided for large-scale planting, development and utilization of gentiana straminea. Therefore, the yield and quality of gentiana straminea are improved.

Description

technical field [0001] The invention relates to the planting field of Gentiana genti, in particular to a test method for the influence of double stress on the physiological characteristics of the growth of Gentiana genus. Background technique [0002] Gentiana is a perennial herb belonging to the Gentianaceae family. Gentiana is also known as Gentiana big-leaf and Gentiana mahua. Tibetan medicine calls it "Jiejigabao". Twist-shaped, so it is commonly called Gentiana twist. As a commonly used traditional Chinese medicine, Gentiana chinensis is also commonly used in Tibetan medicine and Mongolian medicine. It is a key population that constitutes the ecology of the Qinghai-Tibet Plateau and is also a national third-level key protected plant. Due to the increase in clinical use in recent years and years of over-harvesting, the current shortage of wild resources of Gentiana chinensis has been caused. [0003] Large-scale introduction and cultivation has become a sustainable dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/00A01C1/02G01N1/28G01N1/34G01N1/38G01N21/31G01N21/78G01N33/00
CPCA01G22/00A01C1/025G01N33/0098G01N21/314G01N21/78G01N1/286G01N1/34G01N1/38G01N2001/2866
Inventor 何志鹏周志娟胡亚刚唐文强马存德赖普辉王娟邢炎华王仕宝胡传彬
Owner SHAANXI INST OF INT TRADE & COMMERCE
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