Near infrared spectrum detection method for evaluating quality of medicinal material of gentiana straminea maxim

A technology of near-infrared spectroscopy and quality evaluation, which is applied in the field of near-infrared spectroscopy detection of the quality evaluation of the medicinal materials of Radix Fructus Save data extraction time, cover a wide range, and analyze the effect of large sample sizes

Active Publication Date: 2020-04-17
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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Problems solved by technology

[0003] Commonly used methods for quality testing of Chinese medicinal materials include high-performance liquid chromatography, ultraviolet-visible spectrophotometry, etc. These methods need to separate the mixture first, and then analyze the simple

Method used

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  • Near infrared spectrum detection method for evaluating quality of medicinal material of gentiana straminea maxim
  • Near infrared spectrum detection method for evaluating quality of medicinal material of gentiana straminea maxim
  • Near infrared spectrum detection method for evaluating quality of medicinal material of gentiana straminea maxim

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Embodiment 1

[0027] A near-infrared spectrum detection method for the quality evaluation of Gentiana twisted medicinal material, the method specifically includes the following steps:

[0028] Step 1: Source and treatment of Gentiana twisted medicinal material samples

[0029] Based on the large geographical distribution scale and different ecological gradients of the Qinghai-Tibet Plateau, multi-point sampling was carried out in typical distribution areas, and the collection time was concentrated in the flowering period of plants from July to September; the samples were placed in separate bags, and a total of 373 samples were collected; the samples were brought back After the laboratory, wash it with tap water and ultrapure water respectively, put it in a cool and ventilated place to dry, take the root of the medicinal part, crush it, and set it aside; ℃ oven to dry to constant weight, put in a desiccator for analysis.

[0030] Step 2: Collection and processing of near-infrared spectral d...

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Abstract

The invention relates to the technical field of traditional Chinese medicinal material quality control and identification, in particular to a near infrared spectrum detection method for evaluating thequality of a medicinal material of gentiana straminea maxim. The method comprises the following steps of: processing a gentiana straminea maxim medicinal material sample and acquiring and processingnear infrared spectrum data; extracting gentiopicroside and loganic acid from the sample for content determination by means of high performance liquid chromatography, importing data and correspondingnear infrared spectrum data into software TQ Analysis, and establishing a model by taking the total amount of gentiopicroside and loganic acid as an evaluation index, and carrying out evaluation. Themethod can be used for simply, conveniently, quickly, accurately and efficiently detecting the quality of the medicinal material; when the sample points are arranged, a resource distribution area is wide in coverage, the sample size is large, and the collected samples are systematic, representative and different; related data of a target absorption peak can be rapidly extracted by adopting the infrared spectrum data extraction software, so that 2/3 of data extraction time is saved; and the method can ensure the most original information of the sample and reflect the interaction and overall relationship of the chemical components of the medicinal material.

Description

technical field [0001] The invention relates to the technical field of quality control and identification of traditional Chinese medicinal materials, in particular to a near-infrared spectrum detection method for quality evaluation of Gentiana twisted medicinal materials. Background technique [0002] Twist Gentiana ( Gentiana straminea Maxim. ) is Gentianaceae (Gentianaceae) Gentiana ( Gentiana ) Gentiana group ( Sect. ) is a perennial herb plant that is endemic to the Qinghai-Tibet Plateau. It is one of the four authentic medicinal materials of the Chinese herbal medicine Gentiana chinensis. The 2015 edition of the "Pharmacopia" stipulates that the decoction pieces of Gentiana chinensis are Gentiana chinensis ( Gentiana macrophylla Pall. ), Gentiana ( G. straminea Maxim. ), Gentiana thick stems ( G. crassicaulis Duthie ex Burk. ) or Gentiana ( G. dahurica Fisch. , also known as the dried root of Gentiana xiaoqin). It has the effects of dispelling rheumatism, ...

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Application Information

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IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 孙菁李佩佩栾真杰李朵孟晓萍
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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