Tissue-culture rapid propagation method for Gentiana arethusae

A technology for tissue culture, rapid propagation and gentian can be applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., and can solve problems such as difficulty in breeding gentian in eastern Sichuan.

Active Publication Date: 2019-03-19
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of tissue culture rapid propagation me...

Method used

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  • Tissue-culture rapid propagation method for Gentiana arethusae
  • Tissue-culture rapid propagation method for Gentiana arethusae
  • Tissue-culture rapid propagation method for Gentiana arethusae

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for tissue culture and rapid propagation of gentian gentian in Sichuan, comprising the following steps:

[0024] S1, take the root piece at the junction of the rhizomes of gentian gentian without disease;

[0025] S2, cleaning and sterilizing the root block;

[0026] S3, the root block after cleaning and sterilization is completed is transplanted into the primary culture medium with MS as the basic medium and cultivated, wherein the agar content in the primary culture medium is 6g / L, and the sucrose content is 35g / L;

[0027] After the root block in S4, S3 is cultivated in the primary medium for 35 days, some adventitious buds grow, select the plant with the adventitious bud length of 3-6cm, the plant is taken out and the root block is cut, and each new cut is made. There are 2-3 adventitious buds on the root block;

[0028] S5. Transplant each newly cut root block into a proliferation medium with MS as the basic medium for cultivation, wherein the agar conte...

Embodiment 2

[0035] A method for tissue culture and rapid propagation of gentian gentian in Sichuan, comprising the following steps:

[0036] S1, take the root piece at the junction of the rhizomes of gentian gentian without disease;

[0037] S2, cleaning and sterilizing the root block;

[0038] S3, the root block after cleaning and sterilization is completed is transplanted into the primary culture medium with MS as the basic medium and cultivated, wherein the agar content in the primary culture medium is 6.5g / L, and the sucrose content is 25g / L;

[0039] The root pieces in S4, S3 are cultivated in the primary medium for 40 days, grow some adventitious buds, select the plants with adventitious bud lengths of 3-6cm, take out the plants and cut the root pieces, and make each piece of newly cut There are 2-3 adventitious buds on the root block;

[0040] S5. Transplant each newly cut root block into a proliferation medium with MS as the basic medium for cultivation, wherein the agar content...

Embodiment 3

[0048] A method for tissue culture and rapid propagation of gentian gentian in Sichuan, comprising the following steps:

[0049] S1, take the root piece at the junction of the rhizomes of gentian gentian without disease;

[0050] S2, cleaning and sterilizing the root block;

[0051] S3, the root block after cleaning and sterilization is completed is transplanted into the primary culture medium with MS as basic medium and cultivated, wherein the agar content in the primary culture medium is 6.25g / L, and the sucrose content is 30g / L;

[0052] After the root block in S4, S3 is cultivated in the primary medium for 38 days, some adventitious buds grow, select the plant whose adventitious bud length is 3-6cm, the plant is taken out and the root block is cut, and each new cut is made. There are 2-3 adventitious buds on the root block;

[0053] S5. Transplant each newly cut root block into a proliferation medium with MS as the basic medium for cultivation, wherein the agar content i...

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Abstract

The invention discloses a tissue-culture rapid propagation method for Gentiana arethusae. The method comprises the following steps: (S1) extracting root blocks; (S2) cleaning, disinfecting and sterilizing the root blocks; (S3) transplanting the root blocks into a primary culture medium; (S4) selecting plants with adventitious buds with the lengths of 3cm-6cm, taking out the plants, and cutting root blocks, wherein each cut root block is provided with 2-3 adventitious buds; (S5) transplanting each cut root block into a proliferation culture medium, and carrying out culturing; (S6) carrying outtwo-step cutting on 7-9 plants with the adventitious bus with the length of over 5cm on the same root block: firstly cutting branches with the bud length of over 5cm, and secondly cutting the root blocks; (S7) taking out the plants, singly separating buds with the stem thickness of 1.5mm-2.0mm together with base rhizome tissues, and planting the separated plants into a rooting culture medium, andcarrying out culturing; and (S8) carrying out rooting culture for 25-35 days, integrally taking out the rooting plants, transplanting the rooting plants into a seedling hardening bag, carrying out seedling hardening, and transplanting the rooting plants into a culture container.

Description

technical field [0001] The invention relates to the technical field of plant culture, in particular to a tissue culture and rapid propagation method of gentian gentiana. Background technique [0002] East Sichuan Gentian is a perennial herb of Gentianaceae Gentiana, 10-15 cm high; most of the roots are slightly fleshy and whisker-like; most of the flowering branches are clustered and yellow-green. Flowers solitary on top of branches, sessile. [0003] At present, it is difficult to sow and propagate gentian seeds in eastern Sichuan, and some people have done gentian ramets and cutting experiments. However, the cuttings have not been successful, and the reproduction coefficient of ramets is low, which is of little practical significance. This makes the breeding of gentian in eastern Sichuan difficult. The difficulty issue is still unresolved. SUMMARY OF THE INVENTION [0004] The purpose of this invention is to provide a kind of tissue culture fast propagation method of g...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40
Inventor 吴红芝贺水莲周雯金寿林郭彦兵袁文斌
Owner YUNNAN AGRICULTURAL UNIVERSITY
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