50results about How to "Easy to germinate" patented technology

The invention provides an improved bioassay method for an antagonistic bacterium, namely a double-layer culture medium filter paper sandwich method, which belongs to the technical field of a bioassay method for a strain to be tested which is required to be adopted for biological pesticide development. The antibacterial activity of the antagonistic bacterium on plant pathogenic fungi is determinedthrough the simple and easy procedures of manufacture of a sterile filter paper sheet die, manufacture of an upper culture medium and a lower culture medium, shift-in and shift-out of the sterile filter paper die, culture of the antagonistic bacterium to be tested and the target pathogenic fungi, measurement of experimental results and so on, and the antagonistic bacterium with application value can be reasonable screened out. The simple, convenient and quick novel method for implementing bioassay of the antagonistic bacterium has technological innovation. The method can truly reflect the antibacterial ability of metabolites of the strain, has the advantages of good test stability, good repeatability, cheap materials, simple and convenient operation, short experimental period and so on, can realize large-scale determination of a plurality of antagonistic bacteria on a plurality of the pathogenic fungi, and has good function on research of the biological and ecological stability of theantagonistic bacteria.