Aseptic germination method for lomatogoniumr otatum seeds

Abstract

The invention discloses an aseptic germination method for lomatogoniumr otatum seeds. The method comprises the following steps: (1) cleaning the lomatogoniumr otatum seeds, soaking into 20-50% 84-degree disinfectant for 5-15 minutes, and washing with aseptic distilled water for 3 times to obtain disinfected seeds; (2) inoculating the disinfected seeds obtained in step (1) to an MS culture medium, culturing under the condition that the culture temperature is 23-27 DEG C, the illumination intensity is 1500lux and the illumination time is 12-14 hours every day, and obtaining aseptic test-tube plantlets after the seeds are germinated, wherein 30g / L of sucrose and 3.4g / L of agar are added in the MS culture medium, and the pH value of the culture medium is 5.8. By adopting the aseptic germination method, the germination rate reaches 90-100%, the problem that the lomatogoniumr otatum tissue culture seeds are difficult to germinate is effectively solved, and a foundation is laid for sustainable utilization of lomatogoniumr otatum resources.

Description

technical field [0001] The invention relates to a method for germination of plant seeds, in particular to a method for aseptic germination of styloid flowers. Background technique [0002] Riposte flower is the dried whole plant of Gentianaceae plant Lomatogoniumr otatum (L.) Fries ex Nym. It is distributed in Inner Mongolia, Heilongjiang and other places, and it is an authentic medicinal material of Mongolian medicine in Inner Mongolia Autonomous Region. Bitter in taste, cold in nature, blunt, rough, light and dry. It has the effects of calming "Xie Ri", clearing away heat, invigorating the stomach and healing wounds. It is used for "Xie Ri" fever, plague, influenza, typhoid fever, heat stroke, head fever, liver and gallbladder heat, jaundice, stomach "Xie Ri", typhoid fever and other diseases. Lizhuhua is the traditional key medicine of Mongolian medicine. The name of the Mongolian medicine is Habirigen-Digeda. Shishiweisan and Tumuxiang Shiwei Tangsan are used as the m...

AI Technical Summary

Problems solved by technology

Due to the fact that 100% of the medicinal materials of Riposte are currently sourced from wild resources, coupled with human interference and blind excavation, the damage is extremely serious. The reserves of wild resources of Riposte are decreasing year by year, and a large number of resources are on the verge of exhaustion. Wild resources are becoming more and more Therefore, it is urgent to protect and expand the breeding and cultivation of this rare species.

[0003] During the preliminary breeding and breeding work, it was found that the germination of the seeds of Stylophyllum was extremely difficult, which seriously restricted the supply and quality of seedlings

The production of high-quality seedlings through tissue culture technology has become an effective way to produce seedlings of various plants, but the seeds of styloides are small, and when the tissue culture research of stigmatis is carried out, the seeds are often damaged due to excessive disinfection and cannot germinate. It has become a key factor restricting the mass production of seedlings by tissue culture.