Aseptic germination method for lomatogoniumr otatum seeds

A technology of stylized flowers and seeds, applied in the field of aseptic germination of stylized flowers, can solve the problems of restricting the supply and quality of seedlings, difficulty in seed germination, seed damage, etc., achieve protection and sustainable use, and shorten the germination time of seeds , promote the effect of sustainable use

Inactive Publication Date: 2015-07-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the fact that 100% of the medicinal materials of Riposte are currently sourced from wild resources, coupled with human interference and blind excavation, the damage is extremely serious. The reserves of wild resources of Riposte are decreasing year by year, and a large number of resources are on the verge of exhaustion. Wild resources are becoming more and more Therefore, it is urgent to protect and expand the breeding and cultivation of this rare species.
[0003] During the preliminary breeding and breeding work, it was found that ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for aseptic germination of stylized flower seeds, comprising the following steps:

[0015] (1) Seed disinfection: Rinse the Stylophyllum seeds, put them into 20% 84 disinfectant and soak them for disinfection for 15 minutes, then rinse them with sterile distilled water for 3 times to obtain the sterilized seeds;

[0016] (2) Aseptic germination: inoculate the sterilized seeds obtained in step (1) into MS medium for cultivation, and cultivate them for 15 days at a cultivation temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 12-14 hours / day. The next day, the seeds germinated to obtain sterile test-tube plantlets, in which 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8. According to statistics, the germination rate was 96%.

Embodiment 2

[0018] A method for aseptic germination of stylized flower seeds, comprising the following steps:

[0019] (1) Seed disinfection: Rinse the Stylophyllum seeds, put them into 30% 84 disinfectant and soak them for disinfection for 8 minutes, then rinse them with sterile distilled water for 3 times to obtain the sterilized seeds;

[0020] (2) Aseptic germination: inoculate the sterilized seeds obtained in step (1) into MS medium for cultivation, and cultivate them for 15 days at a cultivation temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 12-14 hours / day. The next day, the seeds germinated to obtain sterile test-tube plantlets, in which 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8. According to statistics, the germination rate was 100%.

Embodiment 3

[0022] A method for aseptic germination of stylized flower seeds, comprising the following steps:

[0023] (1) Seed disinfection: Rinse the Stylophyllum seeds, put them into 40% 84 disinfectant solution and soak them for disinfection for 6 minutes, then rinse them with sterile distilled water for 3 times to obtain the sterilized seeds;

[0024] (2) Aseptic germination: inoculate the sterilized seeds obtained in step (1) into MS medium for cultivation, and cultivate them for 15 days at a cultivation temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 12-14 hours / day. The next day, the seeds germinated to obtain sterile test-tube plantlets, in which 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8. According to statistics, the germination rate was 93%.

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Abstract

The invention discloses an aseptic germination method for lomatogoniumr otatum seeds. The method comprises the following steps: (1) cleaning the lomatogoniumr otatum seeds, soaking into 20-50% 84-degree disinfectant for 5-15 minutes, and washing with aseptic distilled water for 3 times to obtain disinfected seeds; (2) inoculating the disinfected seeds obtained in step (1) to an MS culture medium, culturing under the condition that the culture temperature is 23-27 DEG C, the illumination intensity is 1500lux and the illumination time is 12-14 hours every day, and obtaining aseptic test-tube plantlets after the seeds are germinated, wherein 30g/L of sucrose and 3.4g/L of agar are added in the MS culture medium, and the pH value of the culture medium is 5.8. By adopting the aseptic germination method, the germination rate reaches 90-100%, the problem that the lomatogoniumr otatum tissue culture seeds are difficult to germinate is effectively solved, and a foundation is laid for sustainable utilization of lomatogoniumr otatum resources.

Description

technical field [0001] The invention relates to a method for germination of plant seeds, in particular to a method for aseptic germination of styloid flowers. Background technique [0002] Riposte flower is the dried whole plant of Gentianaceae plant Lomatogoniumr otatum (L.) Fries ex Nym. It is distributed in Inner Mongolia, Heilongjiang and other places, and it is an authentic medicinal material of Mongolian medicine in Inner Mongolia Autonomous Region. Bitter in taste, cold in nature, blunt, rough, light and dry. It has the effects of calming "Xie Ri", clearing away heat, invigorating the stomach and healing wounds. It is used for "Xie Ri" fever, plague, influenza, typhoid fever, heat stroke, head fever, liver and gallbladder heat, jaundice, stomach "Xie Ri", typhoid fever and other diseases. Lizhuhua is the traditional key medicine of Mongolian medicine. The name of the Mongolian medicine is Habirigen-Digeda. Shishiweisan and Tumuxiang Shiwei Tangsan are used as the m...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01H4/00
Inventor 韦坤华李旻辉王晓峰李林轩缪剑华朱虹韦范肖冬韦莹李翠
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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