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116results about How to "Short germination time" patented technology

Method for preparing phalaenopsis plant tissue medium

The invention provides a method for preparing a phalaenopsis plant tissue medium and relates to a plant tissue culture technology. The method for preparing the monstera deliciosa plant tissue medium adopts a tissue culture technology, the formula of the medium consists of a seed germination medium, a differential medium, a strong seedling medium and a rooting medium, and the four media are respectively prepared and applied. The formula of the seed germination medium comprises minimal medium Murashige and Skoog (MS), 6-benzyl aminopurine, 1-naphthaleneacetic acid, activated carbon, cane sugar and agar powder; the formula of the differential medium comprises minimal medium 1/3MS, the 6-benzyl aminopurine, the 1-naphthaleneacetic acid, the activated carbon, banana juice, the cane sugar and the agar powder; the formula of the strong seedling medium comprises the minimal medium MS, the 6-benzyl aminopurine, benzaole-3-butyric acid, hyponex No.1, hyponex No.2, the activated carbon, the cane sugar and the agar powder; the formula of the rooting medium comprises the minimal medium MS, the 1-naphthaleneacetic acid, the banana juice, the activated carbon, the can sugar and the agar powder; and the required medium products are respectively prepared and obtained according to the formulas, the phalaenopsis plant tissue culturing and high power breeding are realized.
Owner:SHANDONG JINQIU SEED IND

Rice product rich in gamma-aminobutyric acid and method for producing the same

The invention belongs to the technical field of rice deep processing, in particular to a production method of a functional rice product which has a special sweet flavor and is rich in Gamma-aminobutyric acid and a product thereof, the rice product is produced by virtue of the activities of high glutamate decarboxylase, amylase and protease in germinated brown rice of an indica type rice or a non glutinous rice. The invention takes the indica type rice or the non glutinous brown rice as a raw material which carries out the steps of cleaning, disinfection, soaking, germination, cleaning, beating into rice milk, heat insulation, transformation, drum drying or spray drying, and the like, to be produced into functional nutrition rice flake or rice powder which is rich in Gamma-aminobutyric acid. Compared with the prior art, the rice product makes full use of the activities of the glutamate decarboxylase, the amylase and the protease in the germinated brown rice; the produced rice product has the special sweet flavor; by calculating based on dry basis, the content of Gamma-aminobutyric acid is high (1,000-1,400mg/100g), and the nutritional value and the digestibility are high. The rice product of the invention can be taken as the functional food for resisting fatigue and reducing blood lipid.
Owner:HUAZHONG AGRI UNIV

Microorganism seaweed liquid fertilizer for promoting growth of toadstool and application of microorganism seaweed liquid fertilizer

InactiveCN108484314AOvercome the problem of inconsistent batch quality before and afterIncrease productionBio-organic fraction processingFungiFiltrationHydrolysate
The invention discloses a microorganism seaweed liquid fertilizer for promoting the growth of toadstool and application of the microorganism seaweed liquid fertilizer. An enzymatic hydrolysate is obtained after low temperature physical treatment, high temperature sterilization treatment and enzymolysis treatment are performed on fresh seaweed in sequence; the obtained enzymatic hydrolysate is loaded into a fermentation tank, a compound microbial agent is added in terms of 3% to 5% of the weight of the enzymatic hydrolysate, a fermentation liquor is obtained through fermentation, a filter liquor which is the microorganism seaweed liquid fertilizer is taken after filtration, and the microorganism seaweed liquid fertilizer is sufficiently and uniformly mixed with potassium humate, compound amino acids, EDTA and chitosan, so as to prepare the liquid fertilizer. The application method of the microorganism seaweed liquid fertilizer comprises the following steps: soil is overturned twice in the period of 20 days before sowing, the microorganism seaweed liquid fertilizer is applied to the soil in terms of 4 kg per mu, and spraying is supplemented after first picking of toadstool, so that sufficient early nutrient supply for toadstool is guaranteed, the beneficial viable bacteria in the soil are activated, the soil fertility is improved simultaneously, and the method is significant forimproving the yield and quality of toadstool and promoting the income increase of farmers.
Owner:LIAONING ACAD OF AGRI SCI

Method for preparing culture strains of Agaricus bisporus (Lange) Sing by fermenting culture materials

The invention discloses a method for preparing culture strains of Agaricus bisporus (Lange) Sing by fermenting culture materials and belongs to the technical field of culture of edible mushrooms. According to the method, 1,500kg of dried wheat straw (or rice straw), 1,500kg of dried cow dung, 25kg of urea, 50kg of lime powder, 30kg of calcium superphosphate, 50kg of gypsum powder and 40kg of calcium carbonate are used in the formula of the culture materials for culture strains of Agaricus bisporus (Lange) Sing. The culture materials are treated with outdoor fermentation and indoor post-fermentation technologies, a plastic basket which is 50cm long, 30 cm wide and 30 cm high is used as a container, and the culture strains of Agaricus bisporus (Lange) Sing are prepared through inoculation and cultivation. Compared with a currently commonly used method for preparing the culture strains from wheat serving as a main culture medium, the method has the advantages of grain saving, labor saving, energy saving, cost saving, shortening of strain preparation time, consistent culture age, short spawn running time after sowing and low probability of pollution of mixed fungi. The wheat straw (or rice straw) can be sufficiently utilized, environmental pollution caused by straw burning is reduced, and ecological balance is maintained.
Owner:ANHUI SCI & TECH UNIV

Method for increasing budding rate of radix paeoniae rubra seeds

The invention discloses a method for increasing the budding rate of radix paeoniae rubra seeds. The method comprises the steps of seed selection, wherein the radix paeoniae rubra seeds which are fulland free of diseases and insect pests are selected, and poured into warm water of 38-40 DEG C to be soaked and washed, the mixture is continuously stirred until the water temperature is lowered to 20-25 DEG C, and the seeds continue to be soaked for 2-3 h, and are afterwards rinsed thoroughly with clean water; the seeds are put into disinfectant liquid of 38-40 DEG C to be disinfected for 10-20 min, and then aired; seed soaking; budding acceleration, wherein the soaked and dried radix paeoniae rubra seeds are stirred uniformly with fine sand or fine soil the volume of which is 2-3 times that of the seeds, after stirring, a proper amount of water is added, so that the moisture content is within 42-45%, then the mixture is laid on a budding acceleration bed with the thickness of 18-23 cm, alayer of woven straw or plastic thin film covers the upper end of the mixture, the temperature is maintained at 26-27 DEG C, and the seeds bud after 12-18 days; sifting and planting. According to themethod for increasing the budding rate of the radix paeoniae rubra seeds, the budding time of the radix paeoniae rubra seeds is short, and the budding rate is high; meanwhile, the water and labor aresaved, management is facilitated, and the economic benefit can also be greatly increased.
Owner:XINGAN MENGJING MONGOLIAN TRADITIONAL CHINESE MEDICINE TECH DEV CO LTD

Wheat malt composite powder and preparation method thereof

The invention discloses a wheat malt composite powder and a preparation method thereof.Wheat malt powder with a natural color, a natural flavor and a natural taste is scientifically compounded with functional products such as lactobacillus plantarum powder, modified dietary fibers, ferment powder and oligosaccharide, so that the trophism, the food safety and the healthcare function of the wheat malt composite powder are effectively improved, and the probiotic property and the quality stability of the wheat malt composite powder are also significantly improved.The wheat malt composite powder is prepared from simple components and is natural in color, flavor and taste, high in nutritive value, strong in healthcare function, high in food safety and long in shelf life, the content of bioactive substances is high, the viable count of probiotics is high, the probiotic property is high, and complete varieties of functional metabolites of probiotics are provided with a high content.Ultrasonic cleaning, instant microwave puffing and germ killing, high-voltage pulse processing, combined biological enzymolysis and soaking and germination accelerating are adopted in the wheat malt preparation process.The soaking and germination time is short, the germination rate is high, the content of bioactive substances is high, the production efficiency is high, environmental pollution is avoided, and environmental friendliness is achieved.
Owner:易健(大连)生物科技发展有限公司

Method for rapidly propagating Camelia sinensis cv. Chongqing pipacha

InactiveCN102577975AHigh sterilization survival rateProliferate fastPlant tissue cultureHorticulture methodsGenotypeBud
The invention discloses a method for rapidly propagating Camelia sinensis cv. Chongqing pipacha. The method comprises the following steps of: sequentially cleaning an explant of a stem segment with buds of an indoor cutting seedling of the Camelia sinensis cv. Chongqing pipacha by using soapy water, soaking in carbendazim, and washing by using running water, completely absorbing the moisture on the surface of the explant, and removing small leaves from outer layers of the buds and the stem segment; sequentially disinfecting the explant by using alcohol and mercury bichloride under aseptic conditions, washing by using sterile water, inoculating into a Murashige and Skoog (MS) culture medium, culturing, and screening out a survival aseptic material; transferring the aseptic material into a startup culture medium, culturing and inducing into buds; and transferring the germinant aseptic material into an enrichment culture medium, and continuing to culture to obtain cluster buds with the average multiplication coefficient of 3.31 to 4.86. By the method, the aseptic material of which the sterilization survival rate is up to 88.5 percent can be obtained, the germination time is short, the multiplication rate is high, the cluster buds of which the average multiplication coefficient is up to 4.86 can be obtained within three months, and excellent characters of a genotype and a phenotype of the original clone can be maintained.
Owner:SICHUAN UNIV +1

A kind of efficient method for in vitro liquid culture of tobacco pollen

The invention relates to a high-efficiency tobacco pollen in-vitro liquid culture method, which mainly comprises the operating steps of rapidly releasing a great amount of pollens by rolling a sterilized anther under the effect of B5-13 liquid culture in sterile conditions, conducting low-speed centrifugal separation to collect the pollens, statically placing the pollens in NLN-16 liquid culture mediums containing colchicines with concentration being 50-70mg / L at 32DEG C in the dark for reduplication, then statically placing the pollens in NLN-13 liquid culture mediums at 25 DEG C for embryo induction culture in the dark, placing embryos into a 25 DEG C constant-temperature shaking bed for oscillatory culture in the dark for 7-10 days after the embryos can be seen by naked eyes, transferring the embryos which are at a cotyledon period into solid culture mediums for subculture, and after seedlings are trained, transplanting the seedlings in the field in a transplanting season till blooming, bagging and fruiting. The high-efficiency tobacco pollen in-vitro liquid culture method has the characteristics that the concept is novel, the design is ingenious, scientific and practical, the high efficiency and the stability are realized, the yield is great, the tobacco breeding cycle can be greatly shortened, and the like.
Owner:TOBACCO RES INST OF HUBEI PROVINCE

Preparation method for rapidly separating tremella fuciformis pure hyphae and tremella fuciformis mother strains

The invention discloses a preparation method of a tremella fuciformis mother strain. According to the method, a wood chip culture medium containing the Xianghuijun strain culture is prepared by separating the Xianghuijun strain hyphae, and then the pure tremella fuciformis hyphae obtained through separation and the Xianghuijun strain hyphae are subjected to cross culture on the wood chip culture medium containing the Xianghuijun strain culture to prepare the tremella fuciformis mother strain. Compared with a traditional method, the separated pure tremella fuciformis mycelia have the advantagesof being short in germination time, robust in growth, pure white, dense and the like; separation of tremella fuciformis pure hyphae and preparation of tremella fuciformis mother strains are completedin the same bottle of wood chip culture medium, operation is easy and convenient, damage to the tremella fuciformis pure hyphae caused by multiple times of transfer and infectious microbe pollution possibly occurring in the transfer process are avoided, meanwhile, the preparation time of the tremella fuciformis mother strains is shortened, and the preparation cost of the tremella fuciformis mother strains is effectively reduced.
Owner:GUANGZHOU GENSOURCE BIO TECH CO LTD

Seedling growing method of bletilla striata

The invention provides a seedling growing method of bletilla striata. The method comprises the following steps: the whole seedling growing process is divided into two stages, wherein in the first stage, seeds are uniformly scattered on the surface layer of a culture medium and germination cultivation is conducted in a tissue culture chamber; and after the seeds are germinated to form protocorms, second-stage cultivation is conducted, the protocorms are stripped from the culture medium and are put into water, the protocorms are scattered on a seedling bed after being dispersed, the protocorms are exposed on the substrate, nutritional liquid is sprayed on the seedling bed at fixed period, moisture preservation, temperature control and shading of the seedling bed are considered, direct sunlight is prevented, and water and fertilizer management as well as disease prevent and control management are conducted. The seedling growing method of the bletilla striata is high in germination efficiency, short in germination time, simple and convenient in management and low in seedling growing cost. Germination can be completed after 10 to 15 days and tissue culture chamber cultivation can be completely after 30 to 35 days. Inoculation on the culture medium is needed only for one time, the protocorms are scattered on the seedling growth substrate after being formed and seedling growing is conducted.
Owner:湘西自治州宏盛生态农业旅游开发有限公司
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