The invention discloses a nutrient solution for picria felterrae seed germination and a preparation method of the nutrient solution and belongs to the technical field of picria felterrae planting. The nutrient solution for picria felterrae seed germination is prepared from the following raw materials: bananas, potassium chloride, sodium phosphate, ammonium bicarbonate, calcium chloride, magnesium glycinate, sodium iodide, potassium borate, sodium molybdate, zinc chloride, manganese methionine, copper chloride, ferrous sulfate, vitamin, amino acid, indolebutyric acid, gibberellin, 6-benzyl adenosine, naphthylacetic acid, kinetin, a cosolvent, a dispersing agent, a stabilizer, an antibacterial agent and water. The nutrient solution for picria felterrae seed germination is prepared through mixing, pH value adjustment and sterilization. Picria felterrae seeds are germinated by adopting the nutrient solution, so that the germination rate is high and the germination time is short.