Method for using Lycoris radiata to induce cluster buds

A technology of safflower lycoris and liquid culture medium, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of slow growth and development of small bulbs, long bud induction period, low survival rate and the like, and achieves germination. The effect of high rate and proliferation rate, short budding time and rapid budding quantity

Inactive Publication Date: 2012-07-18
ZHEJIANG UNIV
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Problems solved by technology

[0003] Although there have been many reports on the in vitro rapid propagation of Lycoris safflower, the problems of pollution and browning are serious, the survival rate is low, and the first-generation germination induction period is long (the germination time generally takes 30 to 40 days, and the germination rate is generally 60%. about), and the growth and development of small bulbs are slow (the diameter increases by about 2.0cm in the first year), these are the bottlenecks that are difficult to break through by the tissue culture method of Lycoris safflower

Method used

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  • Method for using Lycoris radiata to induce cluster buds
  • Method for using Lycoris radiata to induce cluster buds
  • Method for using Lycoris radiata to induce cluster buds

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Embodiment 1

[0025] (1) Take mature Lycoris safflower bulbs (average circumference 13.40cm, average fresh weight 34.89g, average number of scales 15 layers) in spring and summer, and cut them into quarters in the shape of a "ten" with the base plate as the center to obtain Put the bulb pieces in the substrate (the volume ratio of peat and perlite is 3:1) for cutting; after 90 days, each bulb piece produces 1 to 6 small bulbs with a diameter of about 1.0 cm, which can be used as explant materials.

[0026] (2) Remove the roots and remaining female scales from the young bulbs obtained from cuttings, wash them, and cut off the upper 1 / 2 of the bulbs; Soak in 70% ethanol for 30s, then soak in mercuric chloride with a weight volume concentration of 0.1% for 5min, and finally rinse with distilled water.

[0027] (3) The sterilized material is aseptically inoculated into liquid medium, placed in a constant temperature shaker at 25°C and a rotating speed of 100rpm, and cultured in the dark; after ...

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Abstract

The invention discloses a method for using Lycoris radiata to induce cluster buds, which includes: subjecting a bulb of Lycoris radiate to cuttage into a substrate, and culturing for 60-90 days to obtain a tender small bulb; and inoculating the small bulb sterilized to a liquid medium for induced culture; after buds appear in induction, transferring the buds to a solid medium for enrichment culture, and obtaining cluster buds; wherein the liquid medium contains 0.5-1.0mg / L alpha-naphthaleneacetic acid and 3.0-5.0mg / L 6-benzylaminopurine. The bulb of Lycoris radiate is subjected to cuttage propagation to obtain the mall bulb used as explant material, and the explant is tender, convenient in massive obtaining and low in pollution rate. In addition, by induced culture, liquid suspension culture, amount of emergent buds is large, emergency is quick, and a great amount of quality cluster buds can be obtained after one month. Application of the method attains the advantages that budding time is short, budding rate and proliferation rate are high, the small bulb is fast in growth and development, and the like.

Description

technical field [0001] The invention relates to the technical field of rapid plant propagation, in particular to a method for inducing clustered buds of Lycoris safflower. Background technique [0002] The natural reproduction rate of Lycoris safflower is quite low, with an average of only 1 to 2 seed balls produced each year. Tissue culture is a common way to quickly obtain regenerated bulblets of Lycoris safflower, and it is also an effective way to finally achieve mass reproduction. Compared with general reproduction methods, tissue culture is not limited by season and space, and has a high reproduction rate, and an efficient in vitro regeneration system is also the basic condition for further establishment of a genetic transformation system. [0003] Although there have been many reports on the in vitro rapid propagation of Lycoris safflower, the problems of pollution and browning are serious, the survival rate is low, and the first-generation germination induction peri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00A01H4/00
Inventor 常乐夏宜平陈菁珏肖郁绵佘琳芳吴昀张琳
Owner ZHEJIANG UNIV
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