100 results about "Naphthaleneacetic Acids" patented technology

The invention relates to the technical field of rice seed germination accelerating systems and methods. Seed selection is conducted, specifically, rice seeds which are full and free of damage are screened, and impurities are removed; the rice seeds are soaked in a plant growth regulator mixed solution to be subjected to oscillation treatment, the mixed solution is composed of gibberellin with the concentration being 20-50 mg / L, pimacol with the concentration being 5-15 mg / L and polyoxyethylene lauryl ether with the concentration being 0.01-0.03%, the liquid temperature is kept at 30 + / -2 DEG C, and the treatment time is 2-4 hours; gradient disinfection: primary disinfection: putting the pretreated seeds into a 0.3-0.5% hydrogen peroxide solution at the temperature of 40-50 DEG C, and stirring for 15-20 minutes; secondary disinfection: transferring to a 0.8-1.2% sodium hypochlorite solution at the temperature of 25-30 DEG C, adding a 0.05-0.1 mol / L phosphate buffer solution to adjust the pH value to 6.8-7.2, and standing for 40-60 minutes; the disinfected seeds are put into magnetized water containing 0.1%-0.3% of chitosan derivatives and 0.05%-0.15% of sodium alginate, the magnetic field intensity ranges from 300 gauss to 500 gauss, and ultrasonic waves of 30 kHz to 50 kHz are adopted for assisting permeation for 1 h to 2 h; the rice seed germination accelerating system and method are high in germination accelerating efficiency.

The invention belongs to the field of rambutan cultivation, and particularly relates to a special compound fertilizer for rambutan and application thereof. According to the special compound fertilizer for the rambutan, disclosed by the invention, the millettia speciosa champ residues, the bagasse, the mimosa pudica and the fish meal are used as substrates, bacillus velezensis HAB-2 is added for fermentation to prepare an extract, and then fulvic acid, brassinolide and naphthylacetic acid are added to finally prepare the compound fertilizer, so that the growth of rambutan seedlings can be remarkably promoted; on the premise that healthy growth of rambutan seedlings is guaranteed, the seedling raising period of the rambutan is shortened, and the method has important promoting significance on cultivation of the rambutan.

This invention provides an induction culture medium and method for using marigold anthers as explants. The induction culture medium is based on MS solid medium containing agar, with specific amounts of sucrose, tryptophan, phenylalanine, arginine, casein amino acids, anhydrous asparagine, 6-benzylpurine, naphthaleneacetic acid, silver nitrate, pyridoxine hydrochloride, thiamine hydrochloride, nicotinic acid, D-biotin, folic acid, ascorbic acid, and coconut juice added. This invention also provides a method for induction culture using this induction culture medium. This invention can greatly improve the callus induction rate of marigolds, significantly increasing the number of regenerated seedlings, and provides technical guidance for the cultivation of haploid marigolds using anthers.

The invention discloses a method for increasing the content of genistin and genistein in flemingia philippinensis by using potassium. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with the content of genistin and genistein increased; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting culture medium comprises KNO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain KNO3. According to the method for increasing the content of genistin and genistein in the flemingia philippinensis by using potassium, accumulation of active ingredients of genistin and genistein at the root of the flemingia philippinensis is adjusted by adding exogenous potassium ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

This invention relates to a rapid induction culture medium and method for inducing bud formation from kiwifruit leaves to complete plants, belonging to the field of biotechnology. The callus induction culture medium for rapidly inducing bud formation from kiwifruit leaves includes MS medium, sucrose, 6-benzylaminopurine, naphthaleneacetic acid, thidiazuron, and agar, with the following weight ratios: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.000001%-0.000003%, naphthaleneacetic acid 0.0000002%-0.0000008%, thidiazuron 0.000001%-0.000003%, agar 0.006%-0.01%, and the balance being water. The formula for the callus induction bud culture medium is as follows: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.0000005%-0.000003%, naphthaleneacetic acid 0.00000005%-0.0000002%, agar 0.006%-0.01%, with the balance being water. This invention provides a rapid induction culture medium and method for kiwifruit leaves to complete plants, effectively inhibiting problems such as callus waterlogging, browning, and vitrification. It not only increases the number of buds but also improves the quality of buds, which is beneficial to the growth and differentiation of callus tissue, making it easier for it to differentiate into buds, thus laying a solid foundation for subsequent genetic transformation experiments.

This invention provides a method and system for detecting trace naphthaleneacetic acid (NAA) based on terahertz spectroscopy. The method includes determining the target parameters of a target metamaterial sensor, performing simulation based on the target parameters to obtain a target terahertz material, acquiring the NAA sample's spectrum through the target terahertz material to obtain target spectral data, training an initial model using a target feature selection method, and performing dimensionality reduction on the target spectral data based on the trained initial model to obtain dimensionality-reduced spectral data, optimizing the model parameters of the initial model, and further optimizing the initial model based on the optimized parameters and the dimensionality-reduced spectral data to obtain a target detection model, and achieving the residual detection of trace NAA through the target detection model. This invention helps improve the accuracy of pesticide residue detection and also provides a new approach for optimizing terahertz spectral data processing methods.

This invention discloses a method for cultivating long-branch Buddha's Hand fruit by cutting branches. The method uses a special variety called "Sunshine" and cultivates high-quality long-branch Buddha's Hand fruit by controlling the density of fruiting branches, spraying a 1500-fold dilution of 3% naphthaleneacetic acid during the bud stage to induce finger-shaped splitting, spraying a gibberellin-chlorpyrifos-potassium dihydrogen phosphate compound solution during fruit development to synergistically thicken the fruit stalk, adjusting the branch direction in summer to keep the fingertips pointing upwards, and combining this with midday shading. This method results in a 26.7% increase in fruit stalk diameter, a 6.4% increase in the number of fruits with open fingers, a 100.2% increase in the number of long-branch fruits, a 20.0% reduction in the number of fruits with fruit surface burns, and an 8.8% reduction in the number of fruits that fall off after harvest. The number of fresh-cut long-branch marketable fruits increases by 100.2%, and the long-branch marketable fruit rate reaches over 70%. Overcoming the bottlenecks of traditional cultivation such as weak fruiting ability of long branches, thin and weak fruit stalks that are easy to fall off, and easy damage to the fruit surface, the cultivated long-branch Buddha's Hand fruit has the advantages of being resistant to fruit drop, having a smooth fruit surface, having a well-developed fruit shape, and having suitable branches. It can be directly used for cut flower scenarios such as vase decoration and bouquet matching, creating a new category of Buddha's Hand cut fruit and significantly enhancing its ornamental and economic value.

The invention belongs to the technical field of idesia polycarpa planting, and particularly relates to a layering seedling forming method for idesia polycarpa. The moss is treated by using the aqueous solution of naphthylacetic acid and indolebutyric acid to form a rooting medium for layering treatment of branches, so that the rooting rate of current-year branches is 100%, the number of adventitious roots of grown seedlings is large (more than 5), the quality is good (white and 1-2mm), and the transplanting survival rate is more than 90%. And the prepared seedlings grow fast, bear fruits early and have stable hereditary characters. Besides, the method does not need to spend a large amount of time for training professional technicians, common labor service temporary teaching can be completed, labor cost is reduced, the used layering substrate is available, purchasing, allocation and transportation troubles are avoided, layering boxes and coiled aluminum wires are easy to purchase and easy to obtain, cost is low, and seedling raising economic cost is integrally reduced.

The invention belongs to the technical field of alfalfa cuttage, and particularly relates to an alfalfa cuttage method capable of improving the survival rate. The method comprises the following steps: (1) selecting cutting branches: selecting alfalfa plants in a squaring stage as a cutting material, and selecting robust branches without diseases and pests as cuttings; (2) cutting pretreatment: immersing a lower end notch of the cutting into a disinfectant, and then soaking into a naphthylacetic acid aqueous solution; finally, soaking the cuttings into a growth regulator to obtain pretreated cuttings; (3) inserting a cutting medium: inserting the pretreated cuttings into a nutrient solution for water culture; (4) transplanting: transplanting 25-30 days after cuttage. According to the alfalfa cuttage method, the rooting rate is high, the root system is developed, the survival rate of transplanted alfalfa is high, and plants grow strongly.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryonic callus induction method which comprises the following steps: selecting medium-grain coffee leaves as explants for surface sterilization; sequentially carrying out three culture stages, namely a dedifferentiation stage, an embryogenic induction stage and an embryogenic maintenance stage, on the sterilized explant; in the dedifferentiation stage, a first culture medium added with 2, 4-dichlorphenoxyacetic acid is adopted, and culture is performed under a first culture condition; in the embryogenic induction period, a second culture medium added with 2, 4-dichlorphenoxyacetic acid and 6-benzyladenine is adopted, and culture is performed under a second culture condition; in the embryogenic maintenance period, a third culture medium added with 1-naphthylacetic acid and kinetin is adopted, and culture is performed under a third culture condition; according to the method disclosed by the invention, the compact embryonic callus with low waterlogging degree is obtained, and the induction rate of the embryonic callus reaches 85-95% through three-stage progressive hormone regulation and control, which is obviously higher than that of the prior art by 30-50%.

The present application relates to the technical field of plant culture, and specifically discloses a method for breeding tagetes and a culture medium.The method for breeding tagetes comprises the step of culturing explants of tagetes into rooted seedlings;an induction culture medium used in the culture of the explants comprises quercetagetin, 6-benzylaminopurine and naphthaleneacetic acid;wherein the mass ratio of quercetagetin, 6-benzylaminopurine and naphthaleneacetic acid is (10-20):(0.1-1):(0.01-0.1);and / or a rooting culture medium used in the culture of the explants comprises quercetagetin and naphthaleneacetic acid;wherein the mass ratio of quercetagetin and naphthaleneacetic acid is (10-20):(0.01-0.1).The method can obtain a large number of tagetes seedlings in a short time, is simple to operate, has a short culture period, is low in input cost, high in propagation efficiency, and suitable for large-scale production and application.

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

The invention relates to the technical field of seed treatment agents, in particular to a late-sowing rape cold-resistant growth-promoting seed-accompanying agent and a preparation method thereof. The seed accompanying agent comprises an effective component, an auxiliary agent and a dispersion medium, effective components comprise a liquid microbial agent, 98% alpha-pimacol, compound sodium nitrophenolate, uniconazole and potassium silicate. The auxiliary agent comprises a mixed orange essential oil auxiliary agent; the dispersion medium comprises deionized water; the liquid microbial agent contains bacillus subtilis, bacillus licheniformis and brevibacillus laterosporus, and the effective viable count is greater than or equal to 2.0 billion / mL. When the seed accompanying agent is used for soaking or dressing rape seeds, the problems of low emergence rate, few strong seedlings and difficulty in overwintering of the rape under the delayed sowing condition are solved, and the seed accompanying agent has important significance in vigorously developing and utilizing winter fallow fields, expanding the rape planting area, improving the rape yield in China and guaranteeing the edible oil supply safety in China.

The invention relates to the technical field of plant cultivation, in particular to a eucommia ulmoides tissue culture seedling rooting induction and large-scale rapid propagation cultivation method which comprises the following raw materials: a modified basic culture medium, modified naphthylacetic acid, modified sucrose, inositol, activated carbon and agar. According to the invention, the modified basic culture medium converts a traditional nutrient medium into an active substrate with biological stimulation and signal initiation functions; the modified naphthylacetic acid realizes stable slow release and biological activation of the auxin, and provides a lasting and mild induction signal; the modified sucrose has a multifunctional carbon source for stress resistance protection, mineral supply and physical support; under the synergism of the three, a continuous action period of starting, inducing and protecting is formed, the structure and the air permeability of a rhizosphere microenvironment are jointly optimized in space, endogenous / exogenous signals are integrated in a physiological level, a key enzyme system is activated, and the cell stress resistance is enhanced, so that the rooting rate, the root system quality and the transplanting survival rate of eucommia ulmoides tissue culture seedlings are synergistically and remarkably improved.

This invention provides a peach asexual fruit-setting agent and a method for peach asexual fruit setting, belonging to the field of fruit tree cultivation technology. The peach fruit-setting agent is composed of gibberellin GA. 4+7 This fruit-setting agent is formulated with chlorpyrifos, naphthaleneacetic acid, and water, with a mass ratio of 80-150:6-12:4-8. Spraying this agent onto the peach tree canopy during peak flowering enables asexual fruit setting. It is suitable for peach non-dormant off-season cultivation, open-field pollen-free peach varieties, and peach cultivation scenarios without natural insect pollination under greenhouse conditions. Through the scientific formulation and synergistic effect of these three plant growth regulators, it effectively solves the problem of low pollen viability and poor natural fruit setting rate caused by high temperatures during the flowering period in non-dormant peach cultivation, reducing the reliance on artificial pollination in conventional greenhouse peach cultivation and significantly lowering labor costs. Practical application has verified that this invention exhibits excellent fruit setting rate, is easy to operate, and has high safety, effectively improving the overall benefits of the peach planting industry and facilitating large-scale promotion and application.

The invention discloses a method for improving vine climbing during transplanting of glabrous greenbrier rhizome seedlings, and belongs to the technical field of planting of traditional Chinese medicinal materials. The method solves the problems of weak stress resistance, low survival rate, long rejuvenation period, low climbing rate in the current year due to improper management and prolonged production period in the transplanting of the glabrous greenbrier rhizome seedlings. According to the technical scheme, the method sequentially comprises the following steps that robust seedlings with proper specifications are obtained; 5-7 days before transplanting, a compound seedling hardening liquid medicine at least containing naphthylacetic acid, monopotassium phosphate and carbendazim is sprayed to enhance resistance; carrying out field planting with an original substrate in a proper period in spring, and irrigating rooting water containing monopotassium phosphate; after transplanting, timely topdressing is carried out by controlling the soil humidity, and when seedlings grow to a certain height, a support is erected in time, and vines are manually guided. The method is used for artificial standardized planting of rhizoma smilacis glabrae, the transplanting survival rate is increased, healthy growth of plants is promoted, high-proportion vine climbing in the first growing season is facilitated, the production cycle is shortened, and planting benefits are improved.

The present application provides a water-soluble plant wound healing agent and a preparation method. The preparation raw materials of the healing agent include water, water-soluble lanolin, mussel mucopolysaccharide, sodium naphthaleneacetate, willow fresh branch extract and copper sulfate. The healing agent can form a thin film with strong water retention and air permeability when sprayed on the wound of the tree body, preventing water evaporation in the wound xylem, inducing the cambium cells to split faster and promoting the rapid growth of the cambium. Meanwhile, the healing agent can prevent external bacteria from entering the wound of the tree body, achieving the effects of bacteriostasis and sterilization, and avoiding secondary damage caused by pathogenic bacteria infection. After the wound is recovered, the water-soluble lanolin disappears due to volatilization and rainwater flushing, avoiding the burn caused by the invasion of oily substances into the bark. The healing agent has good liquid flowability, film-forming property and water retention, and the raw materials are simple and easy to obtain. The preparation process is simple, and the healing agent can be used to heal the wound under complex conditions such as high position, large area and large quantity by means of a spray pot, a small sprayer and the like, greatly improving the work efficiency.

This invention discloses a method for preparing a hydrazine probe for detecting hydrazine in wastewater, relating to the field of wastewater detection technology. The method includes thoroughly mixing 2.0 g of 2,4-dihydroxybenzaldehyde and 3.0 g of 2-naphthaleneacetic acid in 20 mL of acetic anhydride, and slowly adding 2 mL of triethylamine. Under nitrogen protection, the mixture is heated under reflux for 8 hours, cooled to room temperature, and a pale yellow solid precipitates. After filtration, a crude product is obtained, which is then recrystallized in ethanol to obtain 3.75 g of a pale yellow solid. The TTAM probe is accurately weighed and diluted with dimethyl sulfoxide (DMSO) to prepare a probe stock solution with a concentration of 1.0 mmol / L. For testing, 20 μL of the stock solution is transferred and diluted to 2 mL with DMSO solution (V(DMSO):V(H2O) = 2:8). An appropriate amount of N2H4 is accurately drawn using a microsyringe and diluted with distilled water to a detection solution of 1.0 mmol / L. Furthermore, at ℇ = 350 M... ‑1 ·cm ‑1 The absorbance at 333 nm ultraviolet absorption is detected. The probe of this invention has high sensitivity, good selectivity, and short response time. The color of the solution changes significantly before and after reacting with N2H4, enabling "naked-eye detection" of N2H4.

This invention relates to the field of sugarcane cultivation technology, specifically disclosing a method for improving the survival rate of sugarcane seedlings raised from buds. The method includes: cultivating virus-free healthy seedlings using first-generation or second-generation seed stems until they grow to 15-17 buds; removing the sugarcane buds to obtain sugarcane bud segments; disinfecting the sugarcane bud segments; soaking them in a first soaking agent, which consists of the following components by weight: 30-80 parts chitosan-zinc complex, 10-50 parts borax, 0.08-0.2 parts potassium indolebutyrate, 1-3 parts sodium nitrate-naphthaleneacetic acid complex, 0.3-1 part quercetin, 40-80 parts polyvinyl alcohol, 30-60 parts sodium hydroxymethyl cellulose, and 10,000-20,000 parts water; then soaking them in a second soaking agent for a second stage; and finally raising the seedlings. The method of this invention can promote the germination of sugarcane buds, increase the emergence rate of sugarcane seedlings raised by bud removal, improve the quality of sugarcane seedlings, promote the growth of sugarcane seedlings, shorten the seedling raising cycle, and improve the uniformity of emergence, which is conducive to the promotion and application of sugarcane seedling raising and transplanting technology.

The invention provides a rapid seedling raising method for pseudo-ginseng tissue culture, which comprises the following steps: (1) direct induction of explants: selecting leaves of pseudo-ginseng tissue culture seedlings or field seedlings as explants, tearing off petioles, lightly pressing wounds to enable the cuts to be in contact with the surface of a culture medium, carrying out culture by adopting a direct induction culture medium, skipping a callus stage, and taking the explants as explants; directly generating bud points; (2) anti-browning and hormone dynamic regulation and control: adding an explant incision protective agent into the direct induction culture medium, wherein the explant incision protective agent comprises activated carbon, polyvinylpyrrolidone and a silicon element concentrated solution; regulating and controlling the hormone proportion in stages; in the primary induction stage, adding salicylic acid and naphthylacetic acid; in the secondary induction stage, single naphthylacetic acid is adjusted; in a rooting stage, indolebutyric acid and kinetin are combined. The method mainly solves the problems of long period and high browning rate in panax notoginseng tissue culture.

The present invention provides a propagation matrix and a propagation method for promoting spore production in arbuscular mycorrhizal fungi, comprising strigolactones and naphthaleneacetic acid. The present invention utilizes the synergistic effect of strigolactones and naphthaleneacetic acid, uses earthworm castings and peat as primary raw materials, and combines vermiculite, indoleacetic acid, humic acid, and calcium magnesium phosphate to prepare the arbuscular mycorrhizal fungi propagation matrix. The matrix effectively increases the root infection rate of arbuscular mycorrhizal fungi and the number of arbuscular mycorrhizal fungi spores produced. The arbuscular mycorrhizal fungi propagation matrix provided by the present invention is low-cost, simple to operate, and capable of large-scale production and application of arbuscular mycorrhizal fungi inoculants.

The present invention belongs to the field of plant tissue culture technology, and specifically relates to a method and application for inducing callus tissue of aseptic monkey earring seedlings. The method comprises the following steps: taking the root system of the aseptic monkey earring seedlings as an explant and inoculating it in an induction medium for cultivation; the induction medium is an MS medium containing sucrose and agar, to which 0.4-0.6 mg / L of 6-benzylaminopurine, 0.1-0.5 mg / L of naphthaleneacetic acid, and 0-0.3 mg / L of gibberellins are added, and the pH is 5.8-5.9. The aseptic monkey earring seedlings cultivated by the present invention have lush branches and leaves, vigorous root growth, and have the advantages of convenient material collection and low contamination rate. The method can efficiently cultivate monkey earring root callus tissue, provide a reference for the asexual breeding of monkey earrings, and lay the foundation for inducing complete plants and realizing large-scale production of monkey earrings in the future.

The application discloses a cutting treatment method for improving the rooting rate of Buddleja officinalis, and belongs to the technical field of plant cutting propagation. The method comprises the following steps: S1, cutting pretreatment: placing the base of Buddleja officinalis cutting in an ultrasonic generator for ultrasonic pretreatment; S2, rooting agent treatment: immersing the base of the pretreated cutting in a composite rooting agent solution, wherein the composite rooting agent comprises naphthaleneacetic acid, indolebutyric acid and vitamin B1; S3, cutting and maintenance: cutting the treated cutting in a special substrate, and performing post-cutting management. The application improves the cell permeability through ultrasonic treatment, promotes root growth in cooperation with the composite rooting agent with a specific formula, and optimizes the rhizosphere environment in cooperation with the special substrate, thereby improving the rooting rate, the number and quality of the root system of Buddleja officinalis, solving the problems of low rooting rate and poor rooting quality of the existing cutting method, and having the advantages of simple operation, good rooting effect and high seedling raising efficiency.

The application discloses a kind of nano inhibitors, functional coating and its preparation method and application, belong to material technical field.The provided nano inhibitor is used in functional coating, the nano inhibitor includes the following components by mass fraction: nano oxide 5~15 parts, naphthalene acetic acid 1~3 parts, silane coupling agent 0.5~2 parts, dispersing agent 0.2~1 part, and the rest raw material is solvent.A kind of functional coating includes: nano inhibitor, matrix material, functional auxiliary agent and organic solvent;Wherein the nano inhibitor is used to inhibit the growth of weeds and / or vines.The application can alleviate the problem that current protective fence does not have the function of inhibiting the growth of weeds, effectively control the growth of weeds / vines, reduce the frequency of manual weeding operation, and reduce the safety risk.

The present application relates to a kind of seedling substrate and its preparation method, it relates to crop planting technical field, a kind of seedling substrate, including hydrogel microspheres and composite substrate component, the composite substrate component includes: peat, vermiculite, perlite, humic acid, zeolite powder, slow-release fertilizer particle;The composition of the hydrogel microspheres includes: soybean protein isolate, sodium carboxymethyl cellulose, naphthalene acetic acid, xanthan gum, genipin, chitosan, calcium chloride, bacillus subtilis bacterial agent;Its preparation process includes hydrogel microspheres preparation, premixing, initial mixing, final mixing step;The seedling substrate and its preparation method protected in the present application, utilize the porous loose structure formed by hydrogel microspheres, this porous structure makes microsphere only slightly expand after absorbing water, while guaranteeing water-retention capacity, guaranteeing ventilation performance, provide stable microenvironment for seedling growth, further improve crop seedling quality.

A method for root-cutting propagation and seedling cultivation and management of Aralia echinocaulis, which method comprises: selecting lateral horizontal roots of a 2- to 3-year-old robust mother plant to prepare cuttings; soaking the cuttings in a compound solution of indolebutyric acid, naphthaleneacetic acid and 6-benzylaminopurine; performing horizontal burying of cuttings using a sterilized composite matrix composed of peat soil, perlite and humus soil at a ratio of 3:2:1; precisely regulating the temperature and humidity, light and matrix water content in an intelligent temperature-controlled greenhouse; applying nutrient solutions with specific nitrogen-phosphorus-potassium ratios in stages, combined with ferrous sulfate and boric acid to prevent etiolation and promote rooting; gradually adjusting environmental parameters to enter a management stage after adventitious buds unfold leaves; and performing gradient seedling hardening after seedlings achieve the standard.

The present invention provides a kiwifruit sprouting agent and a method for cultivating kiwifruit regeneration preparatory branches. The kiwifruit sprouting agent comprises 6-benzylaminopurine, gibberellin, naphthaleneacetic acid, edible oil, water-based polyurethane, and water. Given the same amount of the sprouting agent, the present invention increases the effective usage amount and effective usage time of the sprouting agent, thereby shortening the time it takes for the plant to sprout.

The invention discloses a formula of a broad-spectrum culture medium adapted to sterile sowing of multiple species of Chinese orchid, and relates to the technical field of plant tissue culture, the broad-spectrum culture medium adopts a 1 / 2MS basic culture medium, the dosage of a macroelement mother solution is halved, and the dosage of ferric salt, microelement and vitamin mother solution is kept unchanged; 10 mg of modified graphene, 1.5 mg to 2.5 mg of 6-benayl aminopurine (6-BA), 0.3 mg to 0.8 mg of naphthylacetic acid (NAA), 0.2 mg to 0.8 mg of zeatin (ZT), 0.05 mg to 0.2 mg of indolebutyric acid (IBA), 1.2 g to 2.2 g of lactoalbumin hydrolysate, 0.6 g to 1.0 g of activated carbon, 22 g to 32 g of saccharose and 5.5 g to 7.5 g of agar are added into every 1 L of the basic culture medium. The pH value of the culture medium is adjusted to 5.2-5.8. By adding the modified graphene into the culture medium, germination of Chinese orchid seeds is effectively promoted. Meanwhile, a complete nutrition system and a root system induction mechanism enhance the growth vigor of the seedlings and improve the survival rate of the seedlings, and meanwhile the universality of the culture medium is remarkably improved through the formula of the culture medium.

The invention relates to a rapid breeding method of rosa serrulata for ecological restoration in plateau alpine regions. Comprising the following steps: collecting current-year healthy branches from a plateau area with the altitude of 2000 m or above, trimming the branches into cuttings of 5-10 cm, and sealing and wrapping cuts; the base parts of the cutting slips are sequentially soaked in a 10-15 mg / L naphthylacetic acid solution and a 80-100 mg / L ABT rooting powder solution; cutting the treated cutting slips into a cultivation container, wherein a matrix is formed by mixing soil, sand, perlite, straw, mushroom residues and ceramsite according to the volume ratio of 8: 3: 1: 1: 1: 1; culturing in a greenhouse environment with the relative humidity of 80-90% and the temperature of 25-30 DEG C, and turning to full illumination after 90 days; after 1-2 growing seasons are cultivated, the container seedlings are placed in a plateau greenhouse to be subjected to seedling hardening for 15-30 days, and then the container seedlings with the matrix are transplanted to a plateau planting field. The method solves the problems of insufficient resource development, difficult seed germination, low cuttage survival rate, slow growth and the like of rosa serrulata.

The present invention discloses a kind of bougainvillea anti-shedding agent and its preparation method and application, and relate to the technical field of plant regulator.A kind of bougainvillea anti-shedding agent, first compounding sugar alcohol calcium and α-naphthaleneacetic acid to obtain an efficacy solution, the calcium ion concentration of sugar alcohol calcium in the efficacy solution is 49-51mg / L, and the α-naphthaleneacetic acid concentration is 24-26mg / L, and then adding surfactant tween to the efficacy solution to obtain the anti-shedding agent, wherein the volume ratio of the added tween to the efficacy solution volume is 1:1000.Before bougainvillea is transported, spraying the anti-shedding agent of the present application can effectively reduce the shedding rate of bougainvillea flowers and leaves during and after transportation.Effectively maintain the commerciality of bougainvillea.