14 results about "Naphthaleneacetic Acids" patented technology

This invention relates to a rapid induction culture medium and method for inducing bud formation from kiwifruit leaves to complete plants, belonging to the field of biotechnology. The callus induction culture medium for rapidly inducing bud formation from kiwifruit leaves includes MS medium, sucrose, 6-benzylaminopurine, naphthaleneacetic acid, thidiazuron, and agar, with the following weight ratios: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.000001%-0.000003%, naphthaleneacetic acid 0.0000002%-0.0000008%, thidiazuron 0.000001%-0.000003%, agar 0.006%-0.01%, and the balance being water. The formula for the callus induction bud culture medium is as follows: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.0000005%-0.000003%, naphthaleneacetic acid 0.00000005%-0.0000002%, agar 0.006%-0.01%, with the balance being water. This invention provides a rapid induction culture medium and method for kiwifruit leaves to complete plants, effectively inhibiting problems such as callus waterlogging, browning, and vitrification. It not only increases the number of buds but also improves the quality of buds, which is beneficial to the growth and differentiation of callus tissue, making it easier for it to differentiate into buds, thus laying a solid foundation for subsequent genetic transformation experiments.

This invention discloses a method for cultivating long-branch Buddha's Hand fruit by cutting branches. The method uses a special variety called "Sunshine" and cultivates high-quality long-branch Buddha's Hand fruit by controlling the density of fruiting branches, spraying a 1500-fold dilution of 3% naphthaleneacetic acid during the bud stage to induce finger-shaped splitting, spraying a gibberellin-chlorpyrifos-potassium dihydrogen phosphate compound solution during fruit development to synergistically thicken the fruit stalk, adjusting the branch direction in summer to keep the fingertips pointing upwards, and combining this with midday shading. This method results in a 26.7% increase in fruit stalk diameter, a 6.4% increase in the number of fruits with open fingers, a 100.2% increase in the number of long-branch fruits, a 20.0% reduction in the number of fruits with fruit surface burns, and an 8.8% reduction in the number of fruits that fall off after harvest. The number of fresh-cut long-branch marketable fruits increases by 100.2%, and the long-branch marketable fruit rate reaches over 70%. Overcoming the bottlenecks of traditional cultivation such as weak fruiting ability of long branches, thin and weak fruit stalks that are easy to fall off, and easy damage to the fruit surface, the cultivated long-branch Buddha's Hand fruit has the advantages of being resistant to fruit drop, having a smooth fruit surface, having a well-developed fruit shape, and having suitable branches. It can be directly used for cut flower scenarios such as vase decoration and bouquet matching, creating a new category of Buddha's Hand cut fruit and significantly enhancing its ornamental and economic value.

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

The application discloses a cutting treatment method for improving the rooting rate of Buddleja officinalis, and belongs to the technical field of plant cutting propagation. The method comprises the following steps: S1, cutting pretreatment: placing the base of Buddleja officinalis cutting in an ultrasonic generator for ultrasonic pretreatment; S2, rooting agent treatment: immersing the base of the pretreated cutting in a composite rooting agent solution, wherein the composite rooting agent comprises naphthaleneacetic acid, indolebutyric acid and vitamin B1; S3, cutting and maintenance: cutting the treated cutting in a special substrate, and performing post-cutting management. The application improves the cell permeability through ultrasonic treatment, promotes root growth in cooperation with the composite rooting agent with a specific formula, and optimizes the rhizosphere environment in cooperation with the special substrate, thereby improving the rooting rate, the number and quality of the root system of Buddleja officinalis, solving the problems of low rooting rate and poor rooting quality of the existing cutting method, and having the advantages of simple operation, good rooting effect and high seedling raising efficiency.

The present application relates to a kind of seedling substrate and its preparation method, it relates to crop planting technical field, a kind of seedling substrate, including hydrogel microspheres and composite substrate component, the composite substrate component includes: peat, vermiculite, perlite, humic acid, zeolite powder, slow-release fertilizer particle;The composition of the hydrogel microspheres includes: soybean protein isolate, sodium carboxymethyl cellulose, naphthalene acetic acid, xanthan gum, genipin, chitosan, calcium chloride, bacillus subtilis bacterial agent;Its preparation process includes hydrogel microspheres preparation, premixing, initial mixing, final mixing step;The seedling substrate and its preparation method protected in the present application, utilize the porous loose structure formed by hydrogel microspheres, this porous structure makes microsphere only slightly expand after absorbing water, while guaranteeing water-retention capacity, guaranteeing ventilation performance, provide stable microenvironment for seedling growth, further improve crop seedling quality.

A method for root-cutting propagation and seedling cultivation and management of Aralia echinocaulis, which method comprises: selecting lateral horizontal roots of a 2- to 3-year-old robust mother plant to prepare cuttings; soaking the cuttings in a compound solution of indolebutyric acid, naphthaleneacetic acid and 6-benzylaminopurine; performing horizontal burying of cuttings using a sterilized composite matrix composed of peat soil, perlite and humus soil at a ratio of 3:2:1; precisely regulating the temperature and humidity, light and matrix water content in an intelligent temperature-controlled greenhouse; applying nutrient solutions with specific nitrogen-phosphorus-potassium ratios in stages, combined with ferrous sulfate and boric acid to prevent etiolation and promote rooting; gradually adjusting environmental parameters to enter a management stage after adventitious buds unfold leaves; and performing gradient seedling hardening after seedlings achieve the standard.

This invention relates to the field of agricultural production technology, providing a chitosan-MOFs composite material, its preparation method, and its applications. The raw materials for the chitosan-MOFs composite material include plant growth regulators, MOFs, and chitosan; the plant growth regulators include naphthaleneacetic acid and tryptophan; the chitosan-MOFs composite material includes NT@CS@UZ(s) and NT@CS@UZ(b). This invention successfully prepared a chitosan-MOFs composite material with both sponge and microsphere morphologies, exhibiting dual adsorption-controlled release functions. In the environment, this composite material can efficiently adsorb aluminum ions while intelligently controlling the release of plant growth regulators. The innovative, recyclable technology provided by this invention can significantly improve plant growth rate and yield while reducing usage costs. Based on the adsorption-controlled release dual function of this composite material, its application in other fields is expected to expand.

This invention belongs to the field of agricultural biotechnology, specifically a growth-promoting watermelon seedling substrate for seed and seedling cultivation and its application. It includes basic substrate components, an intelligent slow-release nitrogen source, an intelligent controlled-release phosphorus source, an intelligent controlled-release potassium source, root-promoting active substances, and a stress-resistance modifier. The intelligent slow-release nitrogen source uses a urea-formaldehyde polymer coating structure, triggered by urease secreted by the roots. The intelligent controlled-release phosphorus source uses a hydroxyapatite carrier, activated and released by root organic acids. The intelligent controlled-release potassium source uses an interlayer ion exchange mechanism of layered silicate minerals, driven by the root's active absorption needs. The root-promoting active substances contain indoleacetic acid, naphthaleneacetic acid, and chitosan. The stress-resistance modifier contains calcium ions, silicon ions, and chitosan derivatives. By adopting the above technical solutions, this application achieves precise synchronization between nutrient release and the actual needs of seedlings, significantly promoting root development, enhancing seedling stress resistance, preventing excessive growth, significantly improving transplant survival rate, and shortening the seedling establishment time.

This invention discloses a segmented, two-stage cultivation process for Ganoderma lucidum. The process first involves activating the fungal strain on a solid culture medium with specific components to ensure its robustness. Then, the activated strain is transferred to a liquid culture medium rich in nutrients such as corn flour and wheat bran. By precisely controlling temperature, humidity, and light cycle, and adding naphthaleneacetic acid (NAA) inducers in stages, fruiting bodies are induced. Finally, the fruiting bodies are harvested using vacuum low-temperature drying. This invention overcomes the bottlenecks of long solid-state cultivation cycles and poor quality in liquid-state cultivation. Through segmented and precise control combining physical environment and chemical inducers, the cultivation cycle is significantly shortened to 35-45 days. It can stably obtain Ganoderma lucidum fruiting bodies with regular morphology, high content of key active ingredients (polysaccharide content ≥15%, triterpenoid content ≥3%), and high yield, providing a reliable solution for the industrialized and standardized production of Ganoderma lucidum.

This invention relates to the field of plant tissue culture technology, and in particular to a culture medium for inhibiting browning of Ganoderma lucidum explants and improving callus induction rate, and its application. This invention provides an MS-based culture medium, further comprising 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA), 6-furfurylaminopurine (KT), polyvinylpyrrolidone (PVP), sucrose, and agar, which inhibits browning of Ganoderma lucidum explants and improves callus induction rate. The pH of this culture medium is 5.83-5.85. Examples show that the culture medium provided by this invention can significantly improve the callus induction rate of Ganoderma lucidum. Furthermore, the callus obtained from the callus induction medium can be subcultured to obtain a large amount of loosely textured and stable callus, providing a high-quality "seed source" for the industrial production of Ganoderma lucidum suspension cells.

This invention relates to the field of tissue culture rapid propagation technology, specifically disclosing a method for improving the survival rate of eucalyptus tissue culture seedlings through transplanting and preservation, comprising the following steps: Step S1, pretreatment before transplanting; Step S2, mud bed preparation; Step S3, transplanting and planting; Step S4, packaging and transportation. This invention addresses the issue from two aspects: first, before transplanting, the roots are dipped in a stress-resistant root-dipping agent, which is prepared by adding synergistic components to the agent. These synergistic components are obtained by compounding L-proline, betaine, and salicylic acid in a specific ratio; second, before transportation, the seedlings are soaked in a compound preservative, which is prepared by mixing naphthaleneacetic acid, calcium chloride aqueous solution, and thiophanate-methyl in a specific ratio. Through the synergistic effect of these substances, the impact of environmental pollution before and after transportation on the eucalyptus tissue culture seedlings is reduced, while simultaneously improving the transplant survival rate of eucalyptus tissue culture seedlings after long-distance transportation.

The present application relates to a kind of chemical fertilizer synergistic additive and its preparation method and application, belong to the field of chemical fertilizer additive.The synergist is composed of water-soluble silicate, polyacrylamide, sodium naphthaleneacetate, sodium indoleacetate, modified biochar and composite microbial inoculant.The inoculant is pre-loaded in the porous structure of modified biochar, and then granulated with polyacrylamide etc., to build a physical-chemical-biological synergistic system.The reticular structure of polyacrylamide and the porous structure of biochar form double fixation, to store nutrients and microorganisms;Bacillus mucilaginosus produces acid to promote silicon dissolution, and Bacillus amyloliquefaciens produces antibiotics to inhibit disease;Plant growth regulator promotes root, and root exudates and microorganisms mutual feed.The present application significantly increases yield under the condition of reducing the amount of chemical fertilizer, and improves soil, with the dual effect of increasing yield and improving soil.

The application discloses an out-of-bottle rooting method for bamboo reed tissue culture seedlings, and comprises the following steps: S1, seedling training: carrying out seedling training on the sterile bamboo reed tissue culture seedlings under the condition that the bottle is not opened; S2, induction treatment: transplanting the tissue culture seedlings after the seedling training in S1 into a container filled with a transplanting substrate, and using a naphthaleneacetic acid (NAA) solution with a concentration of 300-700 mg / L to irrigate the substrate to perform rooting induction; and S3, rooting management: placing the transplanted seedlings in a controllable environment for management, keeping high humidity and light shielding conditions, and until rooting is achieved. The application organically combines seedling training, optimized substrate and hormone treatment, and effectively overcomes problems such as a long rooting period, poor rooting quality, low transplanting survival rate and the like of the bamboo reed test tube seedlings in the bottle. The method has simple steps and low cost, can significantly promote the bamboo reed test tube seedlings to rapidly form developed and healthy root systems outside the bottle, the rooting rate can reach 100%, the seedlings are healthy and strong, the transplanting adaptability is extremely high, and the method is suitable for large-scale commercial seedling production of the bamboo reed.

This application proposes the application and method of oryzanol and naphthaleneacetic acid or their salts in promoting cucumber growth. The combined use of oryzanol and naphthaleneacetic acid or their salts can effectively promote cucumber growth, increase the weight and yield of cucumber fruits, and improve cucumber quality, avoiding undesirable phenomena such as uneven cucumber size, easy bending, and deformed fruits. It is suitable for widespread application.