The invention discloses a culture medium for increasing contents of flavonoid compound and polysaccharide of moghania macrophylla tissue culture seedlings. The culture medium is prepared from the following raw materials of sugar, ginkgetin, potassium salt, phosphor salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, iron salt, vitamins, amino acids, auxin, gibberellin, succinimide, cytokinin, kinetin, a conditioner, a dispersant, a synergist, a stabilizer, an antibacterial agent and water. The culture medium for the moghania macrophylla tissue culture seedlings has the advantages that the compounding is reasonable, the comprehensive nutrients are realized, the root promoting and stem producing can be quickly performed, and the transplanting survival rate is increased; the accumulation of the flavonoid compound and the polysaccharide is promoted, and the economic benefit is increased.