60 results about "Genistin" patented technology

Isoflavones are modified by esterification at one or more of the C4′, C5, C6, and C7 positions to promote bioavailability, and especially to enhance solubility over the corresponding unesterified isoflavones. Preferred modifications produce a carboxylic acid hemiester or a phosphate ester which is biologically hydrolysable. Preferred starting isoflavones are genistin and daidzin, and still more preferably comprises an aglycone form such as genistein or daidzein. Esterified isoflavones may be employed therapeutically or prophylactically for a variety of conditions, provided as a dietary supplement, or added to natural or processed food-stuffs. Further preferred uses include incorporation of contemplated compounds into topical formulations, and especially cosmetic topical formulations.

The invention relates to a method for extracting genistin from largeleaf flemingia. The method comprises the following steps: (1) chopping the raw material largeleaf flemingia and performing reflux extraction by use of ethanol water having the concentration of 50-70% to obtain an extract; (2) concentrating the extract obtained in the step (1) and putting on a chromatographic column, wherein the fixed phase in the chromatographic column is macroporous adsorption resin; eluting with an ethanol-water solution in the volume ratio of (15-25): (85-75) as a moving phase and collecting the eluate; and (3) concentrating the eluate obtained in the step (2) into a thick extract, adding ethanol containing 2-3% of glacial acetic acid for thorough dissolving, standing, filtering out insoluble substances, recrystallizing the filtrate, washing crystals by use of ethanol, and drying in vacuum to obtain the genistin. The method has the advantages of rich and easily available raw materials, simple and feasible process, low pollution and short period; and the obtained product is high in purity and prone to large-scale preparation.

The invention is a method to separate main monomer components in soy isoflavone, using the mixture with not less than 40% soy isoflavone content as raw material, silica gel as sorbent, and chloroform-methanol solution as eluting system, its technical steps: preparing sample solution; processing sorbent and placing in column; applying sample, where the added quantity of sample solution is 5-15% of effective volume of separating column; eluting, where the eluent flow velocity is controlled at 0.5-1.5% of effective volume of separating column each minute, and collecting the eluent in sections; qualitatively detecting the collected eluents, respectively and compared with the contrast product; obtaining monomer components, combining the eluents with the same component, and respectively vacuum condensing and drying to obtain four main monomer components: genistein, daidzein, genistin and daidzin. It is detected by high efficiency liquid phase chromatography that each monomer component's content can reach above 90%.

The invention provides a hair-growing agent composition, which is capable of treating exiguous human hair, calvities occurring in females due to lowering of the estrogen concentration along with the aging by using soybean isoflavones aglycone and 6-benzyl amino purine at the same time, and has great calvities prevention effect while having hair-growing effect. The invention provides a hair-growing agent composition containing soybean isoflavones aglycone and 6-benzyl amino purine. An ideal condition is that the soybean isoflavones aglycone is any one from the daidzin, genistin, and the mol ratio between soybean isoflavones aglycone (A) and 6-benzyl amino purine is 1:1000000 - 1000:1 or the like.

The invention discloses a method for quickly determining six soy isoflavones in a bean product by utilizing UPLC (Ultra Performance Liquid Chromatography), and belongs to the technical field of analytical chemistry. The types of the tested bean product comprise soybean milk, bean curd, soy chicken, dried tofu, bean curds, dried beancurd sticks and the like, and the six soy isoflavones such as daidzin, isoflavoues Aglycone, Glycitin, glycitein, genistin and genistein are determined. The method comprises the following steps: firstly extracting the soy isoflavones in the bean product by using a methanol solution, after collecting a supernate fluid, repeatedly extracting residual twice by using methanol, merging the supernate fluids, and carrying out UPLC detection. Quick determination for the composition and content of the six soy isoflavones mainly in the bean product are achieved by adopting UPLC. The method is simple in sample pretreatment, high in analysis speed, high in repeatability and accuracy, and provides an efficient and accurate analytical method means for evaluating and controlling the quality of the bean product.

The invention relates the soybean isoflavone extractive, comprising 25-74wt% genistin, 4-15wt% soybean glycosides, 3.5wt% chrysophanin. The invention also relates the medicinal composition of soybean isoflavone extractive and preparing method.

The invention relates to a method for extracting genistin from largeleaf flemingia. The method comprises the following steps: (1) chopping the raw material largeleaf flemingia and performing reflux extraction by use of ethanol water having the concentration of 50-70% to obtain an extract; (2) concentrating the extract obtained in the step (1) and putting on a chromatographic column, wherein the fixed phase in the chromatographic column is macroporous adsorption resin; eluting with an ethanol-water solution in the volume ratio of (15-25): (85-75) as a moving phase and collecting the eluate; and (3) concentrating the eluate obtained in the step (2) into a thick extract, adding ethanol containing 2-3% of glacial acetic acid for thorough dissolving, standing, filtering out insoluble substances, recrystallizing the filtrate, washing crystals by use of ethanol, and drying in vacuum to obtain the genistin. The method has the advantages of rich and easily available raw materials, simple and feasible process, low pollution and short period; and the obtained product is high in purity and prone to large-scale preparation.

The invention discloses novel beta-glucosidase. The beta-glucosidase is characterized in that a nucleotide sequence is shown as SEQ ID NO.1, and an amino acid sequence is shown as SEQ ID NO.3. The invention also discloses an expression vector containing the novel beta-glucosidase, recombinant beta-glucosidase, and an application of recombinant beta-glucosidase in efficient hydrolyzing of soybean isoflavone glycoside. The novel recombinant beta-glucosidase shows efficient soluble expression in an escherichia coli expression system, can react preferably at a temperature of 45 DEG C under pH (Power of Hydrogen) of 6.0, and is high in heat stability and wide in pH enzymolysis range; and in addition, the recombinant beta-glucosidase can efficiently hydrolyze daidzin and genistin, and the hydrolyzing efficiency is 100%.

The invention belongs to the field of detection, particularly the field of food detection. Specifically, the invention relates to a marker combination used for determining antioxidant activity of a honey sample as well as a method. More specifically, the invention relates to a marker combination used for determining or evaluating antioxidant activity of a honey drug. The marker combination comprises the follower markers: total phenolic acid, total flavonoids, genistin, methionine and 3,4-dihydroxy-benzoic acid. The invention constructs honey antioxidant composition-activity relationship trophic spectrum research methods for the first time by evaluating antioxidant activity of honey at a cellular level, by virtue of multidimensional target analysis of phenolic acid, flavonoids and amino acid and based on a stoichiometry statistic principle and has important significance for enhancing honey nutrition research, improving honey quality, guaranteeing health of a consumer and developing health use of honey in multiple fields of industrial consumer goods such as food and cosmetics.

The invention discloses a pharmaceutical composition for treating optic atrophy and a preparation method thereof. The pharmaceutical composition is prepared from bark of Japanese spicebush, Szechwan-Yunnan sanicle herb, asarinin, genistin, and hederagenin according to a ratio; the pharmaceutical composition may be made into various forms of drug according to conventional pharmaceutical processes to treat optic atrophy significantly.

The invention relates to the technical field of microorganisms and fermentation, and particularly discloses lactobacillus acidophilus, a method for fermenting soybean milk by using the lactobacillus acidophilus, the prepared fermented soybean milk and an application thereof. The preservation number of the lactobacillus acidophilus HAU-FR7 is CGMCC NO.19253. The lactobacillus acidophilus HAU-FR7 isfacultative anaerobic soybean isoflavone reductive conversion lactic acid bacteria; the growth can be performed under the air oxygen condition; the daidzein and the geniposide in the soybean milk canbe reduced into DHD and DHG in the presence of air oxygen; conversion function is stable, the problem of lactobacilli lacking in facultative anaerobic soybean isoflavone reduction conversion is solved, the scavenging capacity of DHD and DHG on DPPH free radicals is remarkably higher than that of daidzein and genistein, and discovery of the functional lactobacilli has a great promotion effect on development and utilization of functional fermented bean products.

The invention relates to a method for preparing genistein, which can increase the content of genistein by culturing streptomyces lateritius through a liquid culture medium containing soybean meal. The method comprises the following steps: by using a liquid fermentation culture medium, adding cultured streptomyces lateriorubidus CGMCC No.21851 at 29 DEG C, fermenting for 3 days, and extracting, concentrating, separating and purifying a fermentation product, thereby obtaining the genistein. According to the method, it is found for the first time that the streptomyces lateritius CGMCC No.21851 can convert genistin into genistein, compared with similar streptomyces lateritius, the streptomyces lateritius has new performance, and the used raw material soybean meal is rich in resource, low in price and easy to obtain. According to the method, the utilization rate of the soybean byproducts is increased, and a new method and way are provided for production and preparation of genistein.

The invention discloses a production method of soy isoflavone with high content of genistin. The production method comprises the following steps: crushing a raw material namely soybean meal, leaching by using ethanol, filtering and screening by using a screening machine, distilling to recover ethanol, precipitating isoflavone by using n-butyl alcohol, filtering to separate out a precipitation solid-phase material generated by the isoflavone in an n-butyl alcohol solution, performing washing separation and purification for the first time, and performing washing separation and purification for the second time. The production method disclosed by the invention has the positive effects that the purity of the obtained soy isoflavone is more than or equal to 90% and the content of genistin is more than or equal to 80%. The content of genistin is improved, and the soy isoflavone with high content of genistin has exact curative effects of improving the development of female secondary sex characteristics, preventing and curing climacteric syndromes, preventing female senile dementia and height atrophy, preventing and curing cancers related to estrogen secretion and the like. Therefore, the misgiving of consumer groups on the medical healthcare function of soy isoflavone is eliminated, and the production method disclosed by the invention can play an active promotion effect on the further development of the soy isoflavone industry.

The invention discloses a preparation method of high aglycone isoflavone soybean milk powder, which belongs to the technical field of food processing. The method comprises the following steps of preparing the soybean milk powder by adding beta-glucosidase and a pecto-cellulose complex enzyme into soybean milk before soybean milk and bean dregs are separated and carrying out preconcentration and spray drying; the prepared soybean milk powder is normal in color and cannot be browned , content of dsupportzein, glycitein and genistin in the soybean milk powder is obviously improved, and health care value of the soybean milk powder is obviously promoted.

The present invention relates to a Soy daidzin composition and its preparation method and application. It provides three compounds containing isoflavone glucoside which can be used for preparing medicine for curing climacteric syndrome due to female hormone deficiency and middle-aged and senile obtiterating angiocardiopathy and cerebrovascular disease. Said composition contains isoflavone glucoside compound, i.e. diadzin, soyabean flaving glucoside and genistin, and three kinds of isoflavone are 90%-100% of the composition, and the ratio of three isoflavone glucoside is 2-3:1-2:3-4. It can be made into various dosage forms of medicine preparation, and its preparation method includes the following steps: extracting soyabean with ethyl alcohol, concentrating, extracting with petroleum ether, vacuum drying to obtain dried powder.

The invention discloses a medicine composition for improving piglet production performance. The medicine composition consists of the following components in parts by weight: 1.8-2.6 parts of daidzin, 0.1-0.3 part of glycitin, 0.8-1.5 parts of genistin, 2.1-3.7 parts of daidzein, 0.1-0.25 part of glycitein and 0.2-0.3 part of genistein. The invention further discloses a preparation method and application of the composition. The medicine composition disclosed by the invention is good in application prospect, and the production performance of piglets can be effectively improved.

The invention discloses a coated tablet and a production method thereof. The coated tablet is prepared from a soybean extract, vitamin C, white granulated sugar, corn starch, maltodextrin, magnesium stearate, lecithin, cacao pigment, lemon yellow, titanium dioxide, polyvinyl alcohol, talc powder and polyethylene glycol 4000. After three months of acceleration tests on the tablet, every index of the tablet does not have remarkable changes, wherein the contents of main effective components of total amount of soy isoflavone, daidzin, isoflavoues aglycone, genistin, genistein and vitamin C change within 10 percent, and the contents of other physicochemical indexes and microbiological indicators also change within 10 percent. The coated tablet produced by the process has excellent stability.

The invention provides a method for simultaneously extracting various kinds of pueraria isoflavones glycoside from a radix puerariae crude extract. The method comprises the following steps that (1) ice water is added into the radix puerariae crude extract, stirred and centrifugally filtered, and a filter cake is obtained; (2) the filter cake is dispersed by using lower alcohol, then a filling agent is added and stirred to be uniform, vacuum drying is conducted, and a stirring sample filling agent is obtained; (3) a fresh filling agent is added into a chromatographic column, and then the stirring sample filling agent is filled onto the chromatographic column; and (4) an eluent with the concentration being incremental is utilized to conduct gradient elution, eluant is collected and subjectedto vacuum concentration, cooling, stirring crystallizing, centrifugal filtration and drying, and genistin, daidzin and puerarin are obtained respectively. The content of genistin obtained by adoptingthe method is as high as 98.7%, and the yield is as high as 92%; the content of daidzin is as high as 98.5%, and the yield is 92%; and the content of puerarin is as high as 99.9%, and the yield is 97%. The method is easy to operate, low in cost, environmentally friendly, and suitable for industrialized production.

The invention relates to a method for separating and purifying soy isoflavone monomer compounds from a soy isoflavone extract, and the method comprises the following steps: dissolving the soy isoflavone extract with methanol; performing chromatographic separation and purification with a SOURCE15RPC column; eluting by taking the methanol-0.1% phosphoric acid as an eluent; collecting the target-component fractions; performing reduced-pressure concentration of the obtained different fractions to recover the solvent to obtain five soy isoflavone monomer compounds including glycitin, daidzin, genistin, daidzein and genistein respectively, wherein the separation purity is high.

The invention discloses a method for quickly measuring six types of soy isoflavones in a feed additive. The six types of soy isoflavones are respectively daidzin, glycitin, genistin, daidzein, glycitein and genistein. The method comprises the following steps of firstly, using 80% ethanol to extract the soy isoflavones in the feed additive product, centrifuging, taking a supernatant, filtering, and performing HPLC (high performance liquid chromatography) detection. The method has the advantages that the contents of six types of soy isoflavones in the feed additive product can be quickly measured; the pretreatment of a sample is simple, the analyzing speed is high, the repeatability and the accuracy are high, and an efficient and accurate analyzing method is provided for evaluating and controlling the quality of the feed additive product.

The invention discloses a soybean isoflavone extract. The soybean isoflavone extract is prepared from, by weight, 1.8-10% of daidzin, 0.1-1% of soybean xanthosine, 0.8-8% of genistin, 2.1-12% of isoflavoues aglycone, 0.1-1% of glycitein and 0.2-2% of genistein. It is shown by effect experiments that productive performance of the piglets is obviously improved after the soybean isoflavone extract is added into feed to feed piglets. Compared with an existing soybean isoflavone product, the extract has remarkable advantages, indexes such as daily gain, average daily feed intake and the feed conversion ratio are higher than those of the commercially available product Meilidun on the same feeding conditions, and the extract has good market prospects in the field of livestock and poultry breeding. The invention further provides a preparing method of the soybean isoflavone extract. The preparing method is simple in extraction technology, short in extraction time, low in extraction temperature, low in energy consumption and beneficial for large-scale production and reduces production cost to the maximum, and solvents in use can be used for fast extracting special components and are small in use amount.

The invention discloses a composition for preventing kidney stone, comprising the following components in parts by weight: 10-70 parts of daidzin, 3-25 parts of genistin, 5-35 parts of glycitin, 0.1-2 parts of daidzein, 0.005-0.05 part of genistein and 0.01-0.1 part of glycitein. The total amount of the components accounts for above 80w / w% of the total weight of the composition.

The invention provides composition with an anti-aging effect and an application of the composition. The composition contains daidzin, daidzein, genistin, genistein and puerarin in the weight ratio of daidzin to daidzein to genistin to genistein to puerarin being (3*10<4>-7*10<4>):(6-10):(0.8*10<4>-1.5*10<4>):(1-3):(80-110). The composition with the anti-aging effect can be used for health care of skin, breast nursing (including breast firmness, breast enlargement and removal of breast wrinkles) and treatment of prostatic hyperplasia, hypercholesterolemia, arteriosclerosis, erectile dysfunction, menopause symptoms and post-menopause symptoms.

A process for obtaining a solid soy isoflavone- and protein-containing isolate in which isoflavones constitute at least 7% of the isolate and are present in predominantly glycoside form, (genistin+genistein) constitute at least 50% of the isoflavones, the weight ratio (genistin+genistein):(daidzin+daidzein) is at least 1.5:1 and the bulk density is at least 0.57 g / ml. The process includes the sequential steps of (a) heating a soy soluble extract (SSE), made by aqueous alcohol extraction of defatted soy solids and evaporation of the alcohol, at acid pH and at a temperature within the range 70°-90° C. to cause sedimentation of an insoluble fraction containing saponin, trypsin inhibitor and isoflavone compounds; (b) cooling the mixture from step (a) to a temperature of not lower than 30° C. and separating the insoluble fraction from supernatant liquid in the mixture of step (a) at a temperature of not lower than 30° C.; and (c) drying the separated insoluble fraction in a drying system adapted to produce a bulk density of at least 0.57 g / ml in the solid isolate.

The invention relates to a koji soybean using alcohol fermentating yeast and product method of the same. In the invention, non-genetically modified soybeans or wheats are subjected to the heat denaturation in a stainless steel fermentation jar, then the alcohol fermenting yeast strains are added and the fermentation is performed for 72 hours, the fermentation is stopped by adding the protease generation strains, the fermentation product is dried by hot blast, thus the health koji soybean or related product, which has increased Genistin, Daidzein and Glycitein content and improved comprehensive hobby, is obtained.

The invention discloses a hyperlipidemia and atherosis treating drug combination. The hyperlipidemia and atherosis treating drug combination comprises an active component and a medically acceptable carrier, wherein the active component is a combination of two or more of puerarin, daidzin, genistin, calycosin-7-glucoside, astragaloside II, astragaloside, formononetin, mulberroside A, morusin, scopoletin, sanggenone C, 1-deoxynojirimycin, peuraria polysaccharides, astragalus polysaccharides and cortex mori polysaccharides. The hyperlipidemia and atherosis treating drug combination can reduce thearea of fatty plaques in the aorta of an atherosis model of an apoE- / -transgenic mouse, and achieve the effects of adjusting hyperlipidemia of the mouse, thereby being capable of curing hyperlipidemia and atherosis.

The invention discloses a pharmaceutical composition for treating advanced cervical cancer and a preparation method thereof. The pharmaceutical composition provided by the invention is prepared by using fructus pittosporum trigonocarpum, colysis hemionitidea, genistin, trachelospermum axillare and ulva conglobata kjellm as raw medical materials according to a proportion, can be prepared into various dosage forms according to a conventional preparation process, and has significant effect in the treatment of advanced cervical cancer.

A chocolate supplemented with natural botanical xenoestrogen and a preparation method thereof. Each piece of chocolate contains a total sum of 20-30mg of soybean genistin and genistein. The chocolate production process includes: preparing materials; testing and confirming the total weight of soybean genistin and genistein in the soy isoflavones; calculating and determining the amount of soy isoflavones according to the total amount of material addition, weight of 5-10g of each piece and total weight of soy genistein and genistin in each piece; mixing and grinding; refining; and then adjusting temperature, casting mold, hardening and demoulding to obtain the chocolate supplemented with natural botanical xenoestrogen. The present invention has the positive meanings of providing an ideal carrier for the soy isoflavones, confirming the natural botanical xenoestrogens of soy genistin and genistein in soy isoflavones, determining the appropriate content of natural botanical estrogen for daily intake by the consumer, and providing a feeding amount formula for the chocolate with different amount of bulk materials, isoflavones with different contents of xenoestrogen, and different piece weight.