The invention provides a method for simultaneously extracting various kinds of pueraria isoflavones glycoside from a radix puerariae crude extract. The method comprises the following steps that (1) ice water is added into the radix puerariae crude extract, stirred and centrifugally filtered, and a filter cake is obtained; (2) the filter cake is dispersed by using lower alcohol, then a filling agent is added and stirred to be uniform, vacuum drying is conducted, and a stirring sample filling agent is obtained; (3) a fresh filling agent is added into a chromatographic column, and then the stirring sample filling agent is filled onto the chromatographic column; and (4) an eluent with the concentration being incremental is utilized to conduct gradient elution, eluant is collected and subjectedto vacuum concentration, cooling, stirring crystallizing, centrifugal filtration and drying, and genistin, daidzin and puerarin are obtained respectively. The content of genistin obtained by adoptingthe method is as high as 98.7%, and the yield is as high as 92%; the content of daidzin is as high as 98.5%, and the yield is 92%; and the content of puerarin is as high as 99.9%, and the yield is 97%. The method is easy to operate, low in cost, environmentally friendly, and suitable for industrialized production.