Method for simultaneously extracting various kinds of pueraria isoflavones glycoside from radix puerariae crude extract

Abstract

The invention provides a method for simultaneously extracting various kinds of pueraria isoflavones glycoside from a radix puerariae crude extract. The method comprises the following steps that (1) ice water is added into the radix puerariae crude extract, stirred and centrifugally filtered, and a filter cake is obtained; (2) the filter cake is dispersed by using lower alcohol, then a filling agent is added and stirred to be uniform, vacuum drying is conducted, and a stirring sample filling agent is obtained; (3) a fresh filling agent is added into a chromatographic column, and then the stirring sample filling agent is filled onto the chromatographic column; and (4) an eluent with the concentration being incremental is utilized to conduct gradient elution, eluant is collected and subjectedto vacuum concentration, cooling, stirring crystallizing, centrifugal filtration and drying, and genistin, daidzin and puerarin are obtained respectively. The content of genistin obtained by adoptingthe method is as high as 98.7%, and the yield is as high as 92%; the content of daidzin is as high as 98.5%, and the yield is 92%; and the content of puerarin is as high as 99.9%, and the yield is 97%. The method is easy to operate, low in cost, environmentally friendly, and suitable for industrialized production.

Description

technical field

[0001] The invention relates to a method for simultaneously extracting multiple kinds of pueraria isoflavone glycosides, in particular to a method for simultaneously extracting multiple kinds of pueraria isoflavone glycosides from pueraria root crude extract. Background technique

[0002] Pueraria lobata is the tuberous root of the leguminous plant Pueraria mirifica or Gange vine, mainly produced in Henan, Hunan, Zhejiang, Sichuan and other places. It has the functions of relieving muscle and reducing fever, promoting body fluid, promoting rash, promoting yang and antidiarrheal. Wild kudzu root contains 12% flavonoids, such as puerarin, daidzin, peanut and other nutrients, as well as protein, amino acid, sugar, isoflavone glycosides and minerals such as iron, calcium, copper and selenium necessary for human body. It is a precious tonic suitable for all ages, and has the reputation of "thousand-year ginseng". Among them, isoflavone glycosides have good nutri...