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Method for separating and purifying soy isoflavone monomer compounds

A technology for separation and purification of soybean isoflavones, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of difficult repeated use, poor strength of separation materials, and low purity of components, and achieve reagent saving , Purity and efficiency improvement, the effect of high purity

Inactive Publication Date: 2015-07-01
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, methods for separating and purifying high-purity compounds from soybeans have been reported, but various methods may adopt multi-step separation, which is cumbersome to operate; or the separation materials used are weak in strength, easily damaged, and difficult to reuse repeatedly; or the separation process Only one or a few soybean isoflavone components can be obtained; or the purity of the obtained components is not high; or the toxic solvent of chloroform is used in the separation process

Method used

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  • Method for separating and purifying soy isoflavone monomer compounds
  • Method for separating and purifying soy isoflavone monomer compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Use 50% methanol--0.1% phosphoric acid as the eluent for isocratic elution, the three components of daidzein, daidzin, and genistin are well separated, and these three monomer compounds can be obtained, daidzein, The two components of genistein remain difficult to elute, and the elution time is very long.

Embodiment 2

[0027] In Example 2, 90% methanol-0.1% phosphoric acid was used as the eluent for isocratic elution, the three components of daidzein, daidzin, and genistin could not be separated, and the two components of daidzein and genistein were well separated. These two soy isoflavone monomeric compound components are available.

Embodiment 3

[0028] Example 3 Use 45% methanol-0.1% phosphoric acid and 90% methanol-0.1% phosphoric acid as the eluent for step-by-step elution, first elute with 45% methanol-0.1% phosphoric acid, and then wash with 90% methanol-0.1% phosphoric acid After removal, five soybean isoflavone monomer compounds, daidzein, daidzin, genistin, daidzein and genistein, can be separated.

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Abstract

The invention relates to a method for separating and purifying soy isoflavone monomer compounds from a soy isoflavone extract, and the method comprises the following steps: dissolving the soy isoflavone extract with methanol; performing chromatographic separation and purification with a SOURCE15RPC column; eluting by taking the methanol-0.1% phosphoric acid as an eluent; collecting the target-component fractions; performing reduced-pressure concentration of the obtained different fractions to recover the solvent to obtain five soy isoflavone monomer compounds including glycitin, daidzin, genistin, daidzein and genistein respectively, wherein the separation purity is high.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for separating and purifying various soybean isoflavone monomer compounds from soybean isoflavone extracts, mainly separating and purifying daidzein, daidzin, genistin, daidzein, Compounds such as genistein. Background technique [0002] Soy isoflavones are flavonoids present in soybeans and other leguminous plants, which are an important class of biologically active substances. Modern pharmacological studies have shown that soybean isoflavones have the effect of preventing and treating cancer, and have obvious therapeutic effects on breast cancer, colon cancer, lung cancer, prostate cancer, skin cancer and leukemia, and can also prevent ovarian cancer, colon cancer, stomach cancer and prostate cancer Occurrence of cancer; has estrogen-like effect, can prevent women's climacteric syndrome; has the effect of preventing cardiovascular disease, lowers cholester...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08C07D311/36C07D311/40
Inventor 谷焕芝孙爱玲柳仁民傅崇岗
Owner LIAOCHENG UNIV
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