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Soybean isoflavone extract and extraction method thereof

A technology of soybean isoflavones and extracts, applied in the field of medicine, can solve the problems of insufficient yield of soybean isoflavones, unsatisfactory extraction technology, high extraction cost, etc., improve the production performance of piglets, facilitate large-scale production, and shorten the extraction time Effect

Inactive Publication Date: 2016-06-01
CHENGDU HUALUO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing extraction techniques are not ideal
CN102838575A discloses a method for extruding and expanding pretreatment to assist extraction of soybean isoflavones in soybean germ. The process includes multiple steps such as extrusion and expanding, enzymatic hydrolysis, ultrasonic extraction, macroporous adsorption resin purification, and freeze-drying. The operation is complicated, The extraction cost is high, and the yield of soybean isoflavones is less than 1.8%
CN101974577A provides another new production process for extracting soybean isoflavone aglycone. Although the final product obtains soybean isoflavone aglycone with a content of about 40%, the extraction process contains 8 main steps, and there are also problems such as complicated operation and high cost.

Method used

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  • Soybean isoflavone extract and extraction method thereof
  • Soybean isoflavone extract and extraction method thereof

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Experimental program
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Effect test

preparation example Construction

[0036] Preparation of standard solution: Accurately weigh an appropriate amount of daidzein, daidzein, genistin, daidzein, daidzein or genistein standard products, dissolve them in methanol ultrasonically, dilute to volume, shake Evenly, the standard solution contains 50ug of standard substance per milliliter.

[0037] Preparation of the test solution: Accurately weigh 0.5 g (accurate to 0.001 g) of the extract sample of the present invention, ultrasonically dissolve it in 100 ml of methanol, dilute to an appropriate concentration, and shake up. Precisely draw 20 μL each of the standard solution and the test solution through the 0.45um filter membrane, inject it into the liquid chromatograph, and measure it.

[0038] 5. Calculation of measurement results

[0039] The content of soybean isoflavones in the sample was calculated according to the following formula:

[0040] ω i = P i × ...

Embodiment 1

[0050] The preparation of embodiment 1 extract of the present invention

[0051] Put 500kg of soybean germ into a 3-ton multifunctional extraction tank, and add 2000 liters of acetone-water mixed solution, wherein the ratio of acetone to water is 3:1 (v / v);

[0052] Heat up to 65°C and soak for 1 hour;

[0053] Continuous extraction for 3.5 hours, keep the temperature at 65°C, the inflow flow rate is 1100 liters / hour, and the outflow flow rate is 1500 liters / hour;

[0054] The obtained extract was concentrated to a density of 1.3 g / mL, and spray-dried to obtain 97.6 kg of soybean extract.

[0055] The yield of the obtained product is 90.5%, wherein the mass percentage of each index component is: daidzein 1.8%, daidzein 0.1%, genistin 0.8%, daidzein 2.1%, daidzein 0.1%, genistein Soybean isoflavones 0.2%, the total content of soybean isoflavones is 5.1%.

Embodiment 2

[0056] The preparation of embodiment 2 extracts of the present invention

[0057] Put 500kg of soybean germ into a 3-ton multifunctional extraction tank, and add 2000 liters of acetone-water mixed solution, wherein the ratio of acetone to water is 3:1 (v / v);

[0058] Heat up to 70°C and soak for 1 hour;

[0059] Continuous extraction for 3.5 hours, keep the temperature at 70°C, the inflow flow rate is 1100 liters / hour, and the outflow flow rate is 1500 liters / hour;

[0060] The obtained extract was concentrated to a density of 1.3 g / mL, and spray-dried to obtain 54.5 kg of soybean extract.

[0061]The yield of the obtained product is 93.2%, wherein the mass percentage of each index component is: daidzein 3.5%, daidzein 0.18%, genistin 1.56%, daidzein 3.47%, daidzein 0.26%, genistein Soybean isoflavones 0.43%, the total content of soybean isoflavones is 9.4%.

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Abstract

The invention discloses a soybean isoflavone extract. The soybean isoflavone extract is prepared from, by weight, 1.8-10% of daidzin, 0.1-1% of soybean xanthosine, 0.8-8% of genistin, 2.1-12% of isoflavoues aglycone, 0.1-1% of glycitein and 0.2-2% of genistein. It is shown by effect experiments that productive performance of the piglets is obviously improved after the soybean isoflavone extract is added into feed to feed piglets. Compared with an existing soybean isoflavone product, the extract has remarkable advantages, indexes such as daily gain, average daily feed intake and the feed conversion ratio are higher than those of the commercially available product Meilidun on the same feeding conditions, and the extract has good market prospects in the field of livestock and poultry breeding. The invention further provides a preparing method of the soybean isoflavone extract. The preparing method is simple in extraction technology, short in extraction time, low in extraction temperature, low in energy consumption and beneficial for large-scale production and reduces production cost to the maximum, and solvents in use can be used for fast extracting special components and are small in use amount.

Description

technical field [0001] The invention relates to a soybean isoflavone extract and an extraction method thereof, belonging to the field of medicine. Background technique [0002] Soybean isoflavones are a type of secondary metabolites formed during the growth of soybeans and other leguminous plants. They are natural flavonoids and have a typical structure of isoflavones. The main components can be divided into free aglycones and conjugated glycosides. Two categories. Soybean isoflavones have various physiological functions such as anti-cancer, anti-cardiovascular disease, and anti-osteoporosis. They have been developed and used as health food and medical health products for many years. Research on livestock and poultry breeding in recent years has shown that soybean isoflavones have significant effects in animal health care, prevention and treatment of animal immunodeficiency, decreased fecundity, slow growth, and low feed utilization. Therefore, if a soybean isoflavone prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/10
Inventor 晨光李晓斌柴世宏李延肖丽
Owner CHENGDU HUALUO BIOTECH CO LTD
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