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Novel arthrobacter gandavensis strains

A technology of Tsarthrobacter and strains, which is applied in the direction of bacteria, medical raw materials derived from bacteria, animal feed, etc., and can solve the problems of reducing the efficacy of antibiotics

Inactive Publication Date: 2017-02-22
AVEATIS ANIMAL NUTRITION SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to avoid selection for resistant bacteria and thus reduce the efficacy of antibiotics in humans, the European Union partially banned antibiotics in animal feed in 1999 and then completely banned them in 2006

Method used

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  • Novel arthrobacter gandavensis strains
  • Novel arthrobacter gandavensis strains
  • Novel arthrobacter gandavensis strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1: Isolation of rumC+ bacterial strains

[0077] Search for culturable strains carrying rumC-like genes from pig cecum and ileum microbiota.

[0078] In the first stage, the bacteria were cultured in the following media:

[0079] -M17: Promote the growth of Lactococcus

[0080] -LB: Allows Bacillus and Enterococci to grow

[0081] -BEA: media for reverse screening of Bacteroides groups

[0082] Clones were then screened for their ability to inhibit the growth of C. perfringens. Thus, the presence of the rumC gene was shown by PCR ( figure 1 ).

[0083] Table 1: List of primers used to amplify different target DNA fragments

[0084]

[0085] Four strains were retained: AP1, AP2, AP3 and AP4.

Embodiment 2

[0086] Example 2: Identification of Retained Strains

[0087] 2.1 rDNA 16S

[0088] The gene fragment encoding rRNA 16S (approx. 1550bp) (corresponding to positions 8-1541 in the E. coli numbering system), followed by sequencing.

[0089] The sequences obtained are aligned in databases using a sequence homology study program of the "BLAST" type.

[0090] Strain AP1 (SEQ ID No.) => 99.45% identical to Arthrobacter ghentii R 5812

[0091] Strain AP2 (SEQ ID No.) => 99.37% identical to Arthrobacter ghentii R 5812

[0092] Strain AP3 (SEQ ID No.) => 99.44% identical to Arthrobacter ghentii R 5812

[0093] Strain AP4 (SEQ ID No.) => 99.31% identical to Arthrobacter ghentii R 5812

[0094] 2.2. PFGE identification

[0095] The strains AP1, AP2, AP3 and AP4 belonging to the genus Arthrobacter must have genetic differentiation at the gene level. The reference technique for the identification of bacterial strains at the intraspecific level is mainly to assemble their chromos...

Embodiment 3

[0097] Example 3: Characterization of strains

[0098] 3.1 Resistance to pH and to bile salts

[0099] Strains were subjected to two treatments to determine their resistance to acidity and to bile salts.

[0100] - Acidity: 0.85% NaCl buffer, pH 2, containing pepsin (1 mg / mL).

[0101] - Bile salt isotonic buffer (1.24% K 2 HPO 4 , 0.76%H 2 PO 4 , 0.1% trisodium citrate, 0.6% [NH 4 ] 2 SO 4 , pH 6.7), containing 0.2% cholate (50% sodium cholate, 50% sodium deoxycholate).

[0102] Table 2: Survival percentages of different strains

[0103]

[0104] BS: bile salts; ND: not determined

[0105] The AP4 strain was better able to resist conditions that mimic the gastric matrix. In general, all strains were more sensitive to bile salts, however, with the exception of the AP1 strain, the other strains survived adequately.

[0106] 3.2 Fermentation parameters

[0107] For the culture supernatants obtained after semi-anaerobic growth in BHI-YH, their fermentation par...

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Abstract

The subject matter of the present invention is a strain of Arthrobacter gandavensis which has an activity against Clostridium perfringens, chosen from the strains AP1 deposited with the DSMZ on 19 February 2014 under number DSM 28444, AP2 deposited with the DSMZ on 19 February 2014 under number DSM 28445, AP3 deposited with the DSMZ on 19 February 2014 under number DSM 28446 or AP4 deposited with the DSMZ on 19 February 2014 under number DSM 28447.

Description

technical field [0001] The present invention relates to novel bacterial strains having benefits as probiotics in animal feed and more particularly in chicken feed. Background technique [0002] Some bacterial strains have the ability to release substances that have a bacteriostatic or bactericidal effect on their competitors. These antimicrobial substances can be of organic nature, such as organic acids or hydrogen peroxide (Ross et al., Int. J. Food Microbiol. 79, 3-16, 2002), or of peptide nature. Moreover, enzymatically synthesized antimicrobial peptides belonging to the class of antibiotics (Mootz et al., Curr. Opin. Chem. Biol. 1, 543-551, 1997; Keating et al., Curr. Bacteriocin-forming peptides produced by the ribosomal pathway (Jacob et al., Ann. Inst. Pasteur (Paris) 84, 222-224, 1953) are notable. [0003] Bacteriocins are of increasing interest in research and industry; particularly in animal husbandry, where they may offer an alternative to antibiotic use (Lucha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C07K14/305C12N15/31A23K10/18A23K20/147A23L33/135A23L33/18A61K35/74A61P1/00C12R1/06A23K20/195
CPCA61K35/74C07K14/305C12R2001/06C12N1/205A23K20/147A23L33/135A23L33/18A23K10/18A61P1/00
Inventor 安格·皮若尔米歇尔·冯斯卡洛琳·米兰德埃斯特尔·德维兰蒂拉姆亚·哈亚特
Owner AVEATIS ANIMAL NUTRITION SA
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