A kind of method for extracting genistin from Dayejinjinba

Abstract

The invention relates to a method for extracting genistin from largeleaf flemingia. The method comprises the following steps: (1) chopping the raw material largeleaf flemingia and performing reflux extraction by use of ethanol water having the concentration of 50-70% to obtain an extract; (2) concentrating the extract obtained in the step (1) and putting on a chromatographic column, wherein the fixed phase in the chromatographic column is macroporous adsorption resin; eluting with an ethanol-water solution in the volume ratio of (15-25): (85-75) as a moving phase and collecting the eluate; and (3) concentrating the eluate obtained in the step (2) into a thick extract, adding ethanol containing 2-3% of glacial acetic acid for thorough dissolving, standing, filtering out insoluble substances, recrystallizing the filtrate, washing crystals by use of ethanol, and drying in vacuum to obtain the genistin. The method has the advantages of rich and easily available raw materials, simple and feasible process, low pollution and short period; and the obtained product is high in purity and prone to large-scale preparation.

Description

technical field

[0001] The present invention relates to the extraction of natural products, in particular to a method for extracting genistin from Echinacea japonicus. Background technique

[0002] Genistin is a kind of isoflavone compound, which has estrogen-like effect in metabolism in the body, and is called phytoestrogens. Genistin is hydrolyzed into genistein under the action of human intestinal flora, and plays a role by competitively binding to estrogen receptors. It is mainly used in the pharmaceutical industry to treat gynecological diseases.

[0003] In the prior art, patent document CN1876671 discloses a soybean isoflavone extract extracted from soybean meal and an extraction method, the extract contains genistin, daidzin and daidzein, and the product provided by the method is A mixture of isoflavone glycosides, and there is no separation method for genistin. At present, there is an urgent need to develop a method for extracting high-purity genistin suitable for...

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