Method for extracting genistin from largeleaf flemingia
A technology for extracting genistin and genistin, which is applied in the field of extraction of natural products, can solve the problems of no separation method for genistin, and achieve the effects of saving reagents, good reproducibility, and simple operation
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Embodiment 1
[0037] Genistin was prepared as follows:
[0038] (1) Cut 20kg of Daye Qianjinba into 2cm-long fragments, and extract twice with 60% ethanol aqueous solution; for the first time, add 160L of ethanol aqueous solution to the raw material, reflux extraction for 2 hours, and filter to obtain the first time Extract and filter residue; add 120 L of ethanol aqueous solution to the filter residue, reflux for extraction for 2 hours, and filter to obtain the second extract; combine the extracts obtained from the two extractions to obtain the extract;
[0039] (2) Concentrate the extract obtained in step (1) to 5L and put it on the chromatographic column. The stationary phase in the chromatographic column is D101 macroporous adsorption resin, and the resin particles with a particle size of 0.3 to 1.25mm in the stationary phase are not less than 95 %; Carry out pre-elution with water of 2 times of chromatographic column volume, abandon the pre-elution liquid; Carry out elution with the et...
Embodiment 2
[0043] Genistin was prepared as follows:
[0044] (1) Cut 20kg of Daye Qianjin into 2cm fragments, and extract twice with 50% ethanol aqueous solution; for the first time, add 150L ethanol aqueous solution to the raw material, reflux extraction for 2.5 hours, and filter to obtain the first time Extract and filter residue; add 110 L of ethanol aqueous solution to the filter residue, reflux extraction for 2.5 hours, and filter to obtain the second extract; combine the extracts obtained from the two extractions to obtain the extract;
[0045] (2) Concentrate the extract obtained in step (1) to 7.5L and put it on the chromatographic column. The stationary phase in the chromatographic column is D101 macroporous adsorption resin, and the resin particles with a particle size of 0.3-1.25mm in the stationary phase are not less than 95%; pre-elute with 2 times the volume of the chromatographic column, and discard the pre-eluent; then elute with ethanol-water solution with a volume ratio...
Embodiment 3
[0048] Genistin was prepared as follows:
[0049] (1) Cut 20kg of Daye Qianjinba into 3cm fragments, and extract twice with 70% ethanol aqueous solution; for the first time, add 170L of ethanol aqueous solution to the raw material, reflux extraction for 1.5 hours, and filter to obtain the first time Extract and filter residue; add 130 L of ethanol aqueous solution to the filter residue, reflux for extraction for 1.5 hours, and filter to obtain the second extract; combine the extracts obtained from the two extractions to obtain the extract;
[0050] (2) Concentrate the extract obtained in step (1) to 7L and put it on the chromatographic column. The stationary phase in the chromatographic column is D101 macroporous adsorption resin, and the resin particles with a particle size of 0.3-1.25mm in the stationary phase are not less than 95 %; Carry out pre-elution with the water of 1.5 times of chromatographic column volume, abandon pre-elution liquid; Carry out elution with the etha...
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