The invention discloses a chilo suppressalis artificial feed and a preparation method and an application thereof. The preparation method comprises the following steps: (1) picking fresh water bamboo stems for chopping, removing green, drying and smashing, and smashing compound vitamin B, vitamin C, baked soybean and flesh corn; (2) putting glucose, saccharose, compound vitamin B and vitamin C into a beaker, adding distilled water to dissolve the mixture to be used as a component A; (3) uniformly mixing weighed soybean meal, malt flour, yeast powder and water bamboo stem powder to be used as a component B; (4) heating agar for melting with water, adding sorbic acid for boiling and cooling, adding choline chloride and cholesterol, and using the mixture as a component C for standby application; (5) pouring the component A into the component B for uniformly stirring, then adding the component C for continuously, fully and uniformly stirring, pouring the mixture into a preservation box, and putting in a refrigerator for preservation at 4 DEG C after completely cooling and solidification. The chilo suppressalis artificial feed has the characteristics of being low in cost, economic and practical, simplicity and convenience for preparation and the like and is suitable for large-scale, long-term, successive transfer breeding of standard tested insects.