30results about How to "Clear standard" patented technology

The invention discloses a composite insulator defect non-destructive detection method, which comprises that A) a frequency sweep microwave signal source is controlled to emit an incident signal, the incident signal is transmitted by a physical probe into a composite insulator, a reflection signal and a transmission signal are generated, and a receiving probe collects the three signals and sends to an analyzer to process; B) the analyzer performs combined calculation according to the amplitudes and phases of the three signals to obtain a scattering parameters S; C) a step D) is performed if one complete frequency sweep detection is completed, the frequency of the incident signal of the frequency sweep microwave signal source is adjusted if one complete frequency sweep detection is not completed, and the process returns to the step A); D) after one complete frequency sweep detection is completed, a series of scattering parameters S are obtained, and a frequency-scattering parameter S curve is drawn; and E) according to the change condition of the curve, the defect of the composite insulator to be detected is judged and estimated. With the composite insulator defect non-destructive detection method of the present invention, the state and the defect of the composite insulator can be precisely and efficiently diagnosed according to the change condition of the scattering parameters of the composite insulator.

The objective of the invention is to disclose a biochemical luminescence quality control method. The method is characterized in that the method includes 1) a step of selecting a quality control product having a concentration of or near a clinical decision value; 2) a step of storing and redissolving the quality control product in the step 1); 3) a step of subjecting a product of the step 2) to sample reserving and reexamination, namely a step of reserving one sample having an abnormal high value in one week for detection if the product is TBA and HCY, reserving one sample having a normal low value in one week for detection if the product is TSGF, detecting a deviation between a new value and a former value, with the detection value being less than 1 / 3TEA if the detection value is above the inflection point, and resolving a decision limit according the minimum detection difference if the detection value is below the inflection point; and 4) performing quality control analysis, determination and processing, and performing quality control analysis before sample detection everyday; and operation is simple, the process is scientific, criterions are definite, practical operation effects are good, and value accuracy, stability and uniformity are ensured, thus ensuring precision of a detection result.

The invention relates to a Chinese medicinal composition for treating hepatic diseases and process for preparation, which consists of charging 6-8 times of water into Sedum sarmentosum 35-65 parts, curcuma root 15-45 parts, radix isatidis 10-30 parts, bupleurum root 10-30 parts, grilling 2 hours, extracting the filtrate, charging 4-6 times of water again, grilling 1 hour, extracting the filtrate, mixing the filtrates and filtering, charging 4-6 times of water into schisandra fruit, grilling 2 hours, extracting the filtrate, charging 3-4 times of water again, grilling 1 hour, extracting the filtrate, mixing the two filtrates and filtering, extracting seeds of schisandra fruit dross, decompressing and drying, disintegrating into fines with 60 mesh sieves, merging the two soups, concentrating the filtrate into thick grease with specific gravity of 1. 10-1. 20, charging schisandra fruit powder and krestin fines 1-4 parts, finally mixing uniformly.

The method of screening high quality spirulina strain for large scale production includes designing primer of 16S-23S rRNA transcription spacer, cloning and measuring 16S-23S rRNAITS sequences shown in SEQ No. 1 of 7 spirulina strains Sp-1, Sp-2, Sp-3, Sp-5, Sp-9, Sp-10 and Sp-15 by means of PCR technology; separating the 7 spirulina strains into two groups, including the first group comprising Sp-3, Sp-5 and Sp-15, and the second group comprising Sp-1, Sp-2, Sp-9 and Sp-10 according to the similarity of the sequences; and measuring the 16S-23S rRNAITS sequence of the candidate strain and comparing with those of the said strain. When the candidate strain may be classified into the first group, it has excellent character and is suitable for large scale production; when the candidate strain may be classified into the first group, it is not suitable for large scale production.

The present invention discloses a generator bearing and bearing bush ball-type contact surface running-in method. The method comprises the steps of: installing and lifting a bearing bush; respectivelyusing two developers with different colors onto the bearing bush and a bearing pedestal to allow a display effect after grinding to be clearer, coating the first-type developer at an inner ball surface of the lower half portion of the bearing pedestal, and coating the second-type developer on an outer ball surface of the bearing bush; determining a grinding amplitude; and comparing developing areas of the two developers on the bearing pedestal, and determining whether the running-in of the two is qualified or not according to qualification requirements defined in advance. The generator bearing and bearing bush ball-type contact surface running-in method makes corresponding standards for each link of the running-in process consisting of installation of the bearing bush, lifting of the bearing bush, the grinding amplitude, the color coating method and the qualification determination so as to improve the contact surface of the bearing bush and the bearing pedestal and improve the bearingsealing, the operation stability and the production efficiency.

The present invention relates to a method for identifying spirulina line production character quality. Said method includes the following steps: selecting four plants of Sp-1, Sp-2, Sp-3 and Sp-5 from abtuse acron spirulina line; utilizing upstream primer PF:5'-CAATACATCTTCG CCGATTT-3' and downstream primer PR: 5'-CGTATTATCGGTAGTCATCGG-3'; in PCR reaction system making reaction: 94 deg.C 5 min, 94 deg.C 30s, 58 deg.C 1 min, 72 deg.C 2 min, 30 cycles; 72 deg.C 8 min; connecting PCR product recovered and purified by DNA gel onto PMD18-T carrier to screen male clone; extracting plasmid and making enzyme incision identification, sequencing the recombinant plasmid containing target fragment; making the cpc HID operon gene sequences of above-mentioned four plants and correspondent sequence of identified spirulina plant undergo the process of multiple sequence comparison and creating phylogenetic tree so as to identify spirulina line character quality.

The invention discloses a method for DNA detection and quality control in fetus chromosome aneuploid detection. The method comprises the steps of 1 sample collecting, saving and quality control, 2 DNAextraction, 3 library construction, 4 library concentration measuring and saving, 5 upper computer sequencing and 6 result analysis. The method for DNA detection and quality control in fetus chromosome aneuploid detection is easy, convenient and flexible to implement, scientific in flow path, accurate in standard and good in practical operation effect. All the links are strict and clear in standard, efficient and strict, and then the trisomy 21 syndrome, the trisomy 18 syndrome and the trisomy 13 syndrome can be subjected to rapid antenatal auxiliary diagnosis.

The invention discloses a method for automatically matching physical examination items, which comprises the steps of first, setting at least one set of specific physical examination feature codes, andpre-matching each option in the set of physical examination feature codes with physical examination items in a database, wherein the corresponding physical examination items in the database include required physical examination items and optional physical examination items; second, selecting options in the physical examination feature codes according to the action condition of an examined person;and third, automatically matching and generating a physical examination package by a physical examination system after the options in the physical examination feature codes are selected, wherein thegeneration process includes repeated physical examination item removing. The method carries out physical examination item selection automatically, the physical examination items are selected in an automatic and standardized manner, the working efficiency is significantly improved, and work errors are reduced. The invention further discloses a method for automatically generating a collective healthexamination evaluation report, which is used for generating a collective health examination evaluation report for organs, enterprises and institutions. The method is simple to operate, good in practical operation effect, capable of saving the cost of manpower and material resources and accurate in summarization and analysis result.

The purpose of the invention is to disclose a biochemical calibration method. The method comprises the following steps: 1, selecting a corresponding calibration substance; 2, preserving and re-melting the calibration substance in step 1 according to a calibration substance preserving technology and a calibration substance re-melting technology; 3, placing the calibration substance in step 2 in a sample holder in a calibrating instrument; 4, checking the calibrating result of the calibrating instrument, and calibrating a recorded and preserved original result; and 5, determining a constant value quality control substance or a routinely used quality control substance, and judging whether the obtained determination result is in an allowed range or not. The calibration substance in the step 2 comprises a calibration substance suitable for a Roche C501 fully-automatic biochemical analyzer. The biochemical calibration method has the advantages of simplicity in operation, scientific flow, clear standards and good practical operation effect, and solves the technical problems of high cost, unscientific flow, unclear standards, poor practical operation effect, high operation difficulty and difficulty in duplication of biochemical calibration methods.

The invention aims at disclosing a biochemical luminescence analysis and calibration method. The method is characterized by comprising the following steps: (1) selecting a corresponding calibration product; (2) operating the calibration product in the step (1) according to a storage method and a re-melting method of the calibration product; (3) putting the calibration product in the step (2) into a sample frame of a calibration instrument; (4) examining a calibration result of the calibration instrument, and correcting record retention of an original result; and (5) measuring a fixed value quality control product or a conventionally used quality control product, and determining whether results are in an allowed range, wherein the calibration product in the step (2) comprises a calibration product applicable to a Roche's E601 electrochemical luminescence analysis meter. The biochemical luminescence analysis and calibration method has the advantages that the operation is simple, the flow is scientific, the standard is defined, the practical operation effect is good, and the technical problems of a biochemical analysis and calibration method that the cost is high, the flow in unscientific, the standard is undefined, the practical operation effect is poor, and the copy operation is difficult are solved.

The invention discloses a DNA (Desoxyribonucleic Acid) specimen collection and quality control method for fetus chromosome aneuploid detection. The method comprises the following steps: I, performingspecimen collection, namely collecting 5.0mL of peripheral blood of a pregnant woman by using a blood collection tube I, and after collection is completed, slightly inverting the blood collection tube, placing the blood collection tube in a refrigerator in time, collecting 10.0mL of peripheral blood of the pregnant woman by using a blood collection tube II, after collection is completed, slightlyinverting the blood collection tube, and preserving at a normal temperature; II, pre-cooling a low speed centrifuge, putting into the blood collection tube II, centrifuging for minutes, sucking supernate blood plasma, transferring into an EP (Eppendorf) tube on an ice box, and marking with corresponding sample numbers; III, pre-cooling a high speed centrifuge till a temperature is stabilized, putting into the EP tube, centrifuging, sucking the supernate blood plasma on the ice box with a gun head pointing to non-leukocyte precipitation part, sub-packaging into the EP tube on the ice box, transferring into the blood plasma, marking sample numbers and blood plasma tube numbers, and preserving in the refrigerator; IV, performing quality control and treatment on the sample preserved in the step III. The method is definite in standard, good in practice operation effect and efficient and rigorous.

The invention discloses a method for quantitative detection of EB virus DNA and relates to the technical field of biological detection. The method for quantitative detection of EB virus DNA comprisesthe steps that a nucleic acid releasing agent is adopted for rapid splitting decomposition, EBV-DNA in a sample is released, different extracting modes are adopted for plasma samples, peripheral bloodsamples, pharyngeal swab samples and negative positive controls and are assisted by components such as PCR reaction liquid, a real-time fluorescent quantitative PCR detection technology is applied ona fluorescent quantitative PCR instrument, and quantitative detection of EB virus DNA is achieved through change of fluorescence signals. An EB virus can be determined qualitatively and quantitatively according to accurate reported values of EBV DNA. The method has the advantages of being rapid, accurate and convenient to operate and the like.

The invention discloses a DNA library construction method for fetus chromosome aneuploid detection. The method comprises the steps of 1, performing physical interrupting on a sample DNA, and obtaininga fragmented DNA solution; 2, performing tail end repairing and magnetic bead purification on the fragmented DNA, and obtaining the purified blunt end DNA; 3, performing joint connection on the bluntend DNA, and obtaining DNA of the joint; 4, performing PCR amplification on the DNA of the joint, and obtaining a library, wherein according to step 3, the blunt end DNA, nuclear-free water, connection buffer solution, dNTP, DNA ligase, P1 joint and label sequences X are sequentially added into the centrifugal tube and mixed to be uniform, and centrifuging and purifying are performed after the reaction; operation is easy, the flow path is scientific, the standard is clear, and the practical operation effect is good.

The invention discloses a data routing platform management system for a financial enterprise. The data routing platform management system comprises a data source management platform; wherein the data source management platform is provided with a data management module, a calling party module, a system service module, an interface supplier module, a monitoring analysis module and an authority control module; the calling party module opens one or more services through the data management module, the data management module provides a unified calling form for the calling party module, when the calling party module initiates service calling, a specific service API is called through the system service module, and the calling party module initiates service calling; and a specific implementation interface is switched through the interface supplier module, and the interface supplier module provides a specific interface. Through the unified management of the data of the data source management platform, the data exchange process and standard in the management system are clarified, and the effective sharing of various data and the continuous monitoring, analysis, feedback and correction of the data quality are realized.

The invention relates to a method for testing and evaluating the energy consumption performance of a steel-composite material anti-collision facility structure. Belongs to the field of road and bridge anti-collision facility testing. According to the step, the steel-composite material anti-collision facility is impacted through the pendulum bob device, and the instantaneous movement speed before the pendulum bob device impacts the steel-composite material anti-collision facility and the instantaneous movement speed after the pendulum bob device impacts the steel-composite material anti-collision facility and is separated are obtained through a speed sensor; the kinetic energy reduction value of the pendulum bob device is obtained through calculation, and the steel-composite material anti-collision facility is evaluated through the kinetic energy reduction value. According to the method, an anti-collision facility energy consumption capacity calculation method based on the instantaneous speed before and after impact and the kinetic energy theorem is provided, and a clear evaluation standard grade is formulated. The evaluation steps are clear and easy to implement, the comparison result is visual, the characteristics of the energy consumption performance of the structure can be fully reflected, the practical requirements of engineering are met, and good guiding significance is achieved for production and development of steel-composite material anti-collision facilities.

The invention belongs to a technology for development and application of spirulina, and aims at providing a method for judging whether spirulina strains can be applied in large-scale aquaculture production. The method comprises the following steps of: carrying out sequencing on ftsZ genes of the identified spirulina strains, and carrying out variance analysis on site numbers and phylogenetic treeconstruction on the basis of a ftsZ gene sequence together with the known spirulina platensis; and if the identified spirulina strains and the ftsZ gene sequence of the known spirulina platensis strains are gathered into one type, showing that the production traits of the identified spirulina strains are good, and the identified spirulina strains can be applied in large-scale aquaculture production. Compared with the conventional method, the new method for identifying the good and bad production traits of the spirulina strains by applying the ftsZ gene sequence not only is simple, fast, standard and accurate, but also is low in cost, and is suitable for large-scale and high-throughput screening.

The invention relates to a steel-composite material anti-collision facility structure impact resistance device and a method for evaluating and measuring the impact resistance of a steel-composite material anti-collision facility structure. Belongs to the field of road and bridge anti-collision facility testing. An anti-collision facility supporting frame of the device is used for arranging a steel-composite material anti-collision facility, a pendulum bob device is located in a pendulum bob limiting device, and the pendulum bob device moves along the pendulum bob limiting device and collides with the steel-composite material anti-collision facility on the anti-collision facility supporting frame; and the data acquisition system is used for acquiring the movement speed of the pendulum bob device and the deformation data of the steel-composite material anti-collision facility. According to the steel-composite material anti-collision facility structure impact resistance device and the evaluation and testing method, a plurality of characteristic indexes are integrated, the standard is clear, the steps are definite, the operability is high, good economical efficiency and generalizability are achieved, and a powerful supplement is provided for the blank of an existing test and evaluation system in the field of anti-collision facilities.

The invention provides a proportioning design method of an asphalt mixture through construction waste. The construction waste asphalt mixture designed through the method is applicable to asphalt surfaces of highways and urban roads. Recycling of the construction waste is achieved, and pollution caused by urban and rural solid waste is reduced. The proportioning design of the construction waste asphalt mixture is conducted through a three-stage design method. Firstly, the mixture gradation design is conducted through a substitution method; and secondly, the best asphalt use amount of the asphalt mixture is determined through a new design method. The second step is divided into three steps that (1) feasible regions of the best asphalt use amount in different gradation schemes are determined through a Fort shellen leakage analysis test and a Cantabro raveling test; (2) according to the two end boundary values and the intermediate value of the feasible regions of the asphalt use amount, the asphalt mixture is formed through three asphalt use amounts; and (3) whether the asphalt mixture meets the basic performance requirements or not is judged. Finally, the asphalt mixture is formed under the condition of the best asphalt use amount, and pavement performance detection is conducted.

The invention discloses a DNA library concentration measuring method for fetus chromosome aneuploid detection. The method comprises the steps of 1, diluting a sample to be tested, preparing a standardproduct, and performing cryopreservation on the sample and the product; 2, taking a centrifugal tube, marking a tube cover, preparing a reaction mixture according to a reaction system in the table, wherein during preparation, according to a needed quantity preparing system for two-time repeated reactions of each sample, the reaction mixture comprises a quantified amplification regent, nuclear free water, a P1 primer and a P2 primer, after preparation is completed, the reaction mixture is oscillated, and instant centrifuging is performed till the tube bottom; 3, adding the reaction mixture prepared in step 2 into reaction tube holes of a reaction plate, and adding 2 L standard products into the standard product reaction holes; 4, sealing the reaction plate, centrifuging the reaction plate,loading the reaction plate on a fluorescent quantitation PCR cycler, the qPCR reaction begins to be executed after setting is performed, and a library concentration measuring result is obtained; operation is easy, the flow path is scientific, the standard is clear, and the practical operation effect is good.

The invention discloses a marking device for target feature extraction in engineering drilling intelligent video analysis. The marking device comprises a hollow cylinder, a silicone tube, two identical spacers and two identical buckles, the colors of the side face of the hollow cylinder comprise the red color and the yellow color, by taking the central cross section of the hollow cylinder as a boundary, a half of the side face of the hollow cylinder is red, the other half of the side face of the hollow cylinder is yellow, the spacers are mounted on the upper bottom face and the lower bottom face of the hollow cylinder correspondingly, the buckles are mounted on the outer sides of the spacers, and the silicone tube is inserted into a cavity of the hollow cylinder. The color of the marking device is determined on the basis of the color designing principle, the problem encountered in target feature extraction in current engineering investigation field operation intelligent video analysisis solved successfully, through the red-yellow bicolor design mode, the accuracy of target feature extraction is improved, and uniqueness is also achieved; and the marking device is simple in overallstructure, novel in design, high in practicability and reusable.

The invention relates to a shallow-buried bias tunnel operation period surrounding rock and lining structure overall stability evaluation method, and belongs to the technical field of tunnel monitoring. Aiming at the operation safety problem a tunnel, a shallow-buried bias tunnel operation period overall stability analysis and evaluation method based on numerical limit analysis is provided, and the method comprises the steps of: obtaining a surrounding rock mass damage judgment standard (limit strain value) through equivalent rock numerical test analysis; then obtaining a damage judgment standard (limit strain value) of the concrete material through literature research and theoretical analysis; and finally, establishing a tunnel simulation analysis model. Under the condition that lining force structural parameters are kept unchanged, the lining force is reduced, the strength parameters of the surrounding rock are continuously reduced, the stable state and instability failure mode of the surrounding rock are analyzed through the limit strain of the surrounding rock, the structure safety state, vulnerable key parts, damage characteristics and instability failure mechanism are analyzed through the limit strain distribution rule of lining concrete, and then an overall stability evaluation method system is formed.

The invention discloses a library construction method for DNA. The method comprises the steps that form processing, sample preparation, reagent and consumable material preparation and instrument preparation are conducted; tail end repairing mix is added, and incubation of an incubator is subjected to tail end repairing; B1 magnetic beads are added for carrying out first-step purification, washingis carried out two times by using 75% of ethyl alcohol, and TE is dissolved to obtain a purified flat-end DNA sample; the sample is transferred to a subpackaged PCR board of a corresponding label, blowing and beating and uniform mixing are carried out, and joint connection is performed; B2 magnetic beads are added for carrying out purification, and 75% of ethyl alcohol is prepared when the sampleis put on a shelf; washing is carried out two times by using the 75% of ethyl alcohol, TE is dissolved and is transferred to the subpackaged PCR board, and blowing and beating and uniform mixing are carried out to conduct PCR; B2 magnetic beads are added for carrying out purification, when the sample is put on the shelf for 8 minutes, 75% of ethyl alcohol is prepared, washing is carried out two times by using the ethyl alcohol, and 50 TE is dissolved; a scientific and reasonable process makes connection activity be strengthened and improves the joint connecting efficiency so that more repairedDNA segments are successfully connected to a joint sequence, amplification is carried out by using a primer complemented with the joint sequence, and a sequencing library with more target amplification segments is obtained.

An information dynamic distribution method includes: a, The base station receives a call linking request and decides if there is an idle service channel, b judging if there are channels with RSSI less than the threshold value, c, selecting several channels which RSSI value less than the threshold and having the maximum priovity values d, refreshing their priority values, e, finding a channel meeting RSSI less than the threshold and having the maximum channel factor value and allocating it to users, or to enter to the next step if can't, f, judging circulation times, it refuses the allocation if can't find suitable channels and returns to step a to repeast the flow.

A method for identifying the quality of tea. The specific tea was screened by detecting the content of epicatechin gallate (ECG) in the tea, and the expression of the phosphorylated protein pERK produced by the extracellular signal-regulated kinase (ERK) in the cells was determined by the water extract of the specific tea. The effect of increasing the level, thus as a basis for judging the quality of tea with specific biological activity. The method is scientific, accurate, and easy to operate, and can effectively identify the quality of tea based on specific biological activity, which is conducive to the standardization of tea quality management based on biological activity as the criterion for the tea market.

The invention aims to discloses a biochemical luminescence water quality test method which is characterized by comprising the following steps: step 1, while a sample is detected or before water is made by using a water purification machine, detecting the electrical conductivity of the water and replacing a filter element and / or a reverse osmosis membrane of a water purifying device when the electrical conductivity exceeds an allowable range; step 2, collecting 20 ml of water for reagent from water in all water purification equipment by using a sterile container, performing microbial assessment and replacing the filter element of the water purifying device if microorganisms in the water exceed the standards. The allowable range comprises the water for the reagent, water for an instrument and ordinary water, and the maximum electrical conductivities are 0.1 microsecond / cm, 1.0 microsecond / cm and 10 microseconds / cm respectively; the microbial standard comprises the water for the reagent and the water for the instrument, and the maximum microbial contents are 10 and 1000 respectively. The biochemical luminescence water quality test method is simple, convenient and flexible to operate, has the advantage of simple operation of a conventional dyeing method and is scientific in process, clear in standard and good in practical operation effect.