30results about How to "Clear standard" patented technology

The invention discloses a composite insulator defect non-destructive detection method, which comprises that A) a frequency sweep microwave signal source is controlled to emit an incident signal, the incident signal is transmitted by a physical probe into a composite insulator, a reflection signal and a transmission signal are generated, and a receiving probe collects the three signals and sends to an analyzer to process; B) the analyzer performs combined calculation according to the amplitudes and phases of the three signals to obtain a scattering parameters S; C) a step D) is performed if one complete frequency sweep detection is completed, the frequency of the incident signal of the frequency sweep microwave signal source is adjusted if one complete frequency sweep detection is not completed, and the process returns to the step A); D) after one complete frequency sweep detection is completed, a series of scattering parameters S are obtained, and a frequency-scattering parameter S curve is drawn; and E) according to the change condition of the curve, the defect of the composite insulator to be detected is judged and estimated. With the composite insulator defect non-destructive detection method of the present invention, the state and the defect of the composite insulator can be precisely and efficiently diagnosed according to the change condition of the scattering parameters of the composite insulator.

The objective of the invention is to disclose a biochemical luminescence quality control method. The method is characterized in that the method includes 1) a step of selecting a quality control product having a concentration of or near a clinical decision value; 2) a step of storing and redissolving the quality control product in the step 1); 3) a step of subjecting a product of the step 2) to sample reserving and reexamination, namely a step of reserving one sample having an abnormal high value in one week for detection if the product is TBA and HCY, reserving one sample having a normal low value in one week for detection if the product is TSGF, detecting a deviation between a new value and a former value, with the detection value being less than 1/3TEA if the detection value is above the inflection point, and resolving a decision limit according the minimum detection difference if the detection value is below the inflection point; and 4) performing quality control analysis, determination and processing, and performing quality control analysis before sample detection everyday; and operation is simple, the process is scientific, criterions are definite, practical operation effects are good, and value accuracy, stability and uniformity are ensured, thus ensuring precision of a detection result.

The method of screening high quality spirulina strain for large scale production includes designing primer of 16S-23S rRNA transcription spacer, cloning and measuring 16S-23S rRNAITS sequences shown in SEQ No. 1 of 7 spirulina strains Sp-1, Sp-2, Sp-3, Sp-5, Sp-9, Sp-10 and Sp-15 by means of PCR technology; separating the 7 spirulina strains into two groups, including the first group comprising Sp-3, Sp-5 and Sp-15, and the second group comprising Sp-1, Sp-2, Sp-9 and Sp-10 according to the similarity of the sequences; and measuring the 16S-23S rRNAITS sequence of the candidate strain and comparing with those of the said strain. When the candidate strain may be classified into the first group, it has excellent character and is suitable for large scale production; when the candidate strain may be classified into the first group, it is not suitable for large scale production.

The present invention relates to a method for identifying spirulina line production character quality. Said method includes the following steps: selecting four plants of Sp-1, Sp-2, Sp-3 and Sp-5 from abtuse acron spirulina line; utilizing upstream primer PF:5'-CAATACATCTTCG CCGATTT-3' and downstream primer PR: 5'-CGTATTATCGGTAGTCATCGG-3'; in PCR reaction system making reaction: 94 deg.C 5 min, 94 deg.C 30s, 58 deg.C 1 min, 72 deg.C 2 min, 30 cycles; 72 deg.C 8 min; connecting PCR product recovered and purified by DNA gel onto PMD18-T carrier to screen male clone; extracting plasmid and making enzyme incision identification, sequencing the recombinant plasmid containing target fragment; making the cpc HID operon gene sequences of above-mentioned four plants and correspondent sequence of identified spirulina plant undergo the process of multiple sequence comparison and creating phylogenetic tree so as to identify spirulina line character quality.

The invention discloses a method for DNA detection and quality control in fetus chromosome aneuploid detection. The method comprises the steps of 1 sample collecting, saving and quality control, 2 DNAextraction, 3 library construction, 4 library concentration measuring and saving, 5 upper computer sequencing and 6 result analysis. The method for DNA detection and quality control in fetus chromosome aneuploid detection is easy, convenient and flexible to implement, scientific in flow path, accurate in standard and good in practical operation effect. All the links are strict and clear in standard, efficient and strict, and then the trisomy 21 syndrome, the trisomy 18 syndrome and the trisomy 13 syndrome can be subjected to rapid antenatal auxiliary diagnosis.

The invention discloses a method for automatically matching physical examination items, which comprises the steps of first, setting at least one set of specific physical examination feature codes, andpre-matching each option in the set of physical examination feature codes with physical examination items in a database, wherein the corresponding physical examination items in the database include required physical examination items and optional physical examination items; second, selecting options in the physical examination feature codes according to the action condition of an examined person;and third, automatically matching and generating a physical examination package by a physical examination system after the options in the physical examination feature codes are selected, wherein thegeneration process includes repeated physical examination item removing. The method carries out physical examination item selection automatically, the physical examination items are selected in an automatic and standardized manner, the working efficiency is significantly improved, and work errors are reduced. The invention further discloses a method for automatically generating a collective healthexamination evaluation report, which is used for generating a collective health examination evaluation report for organs, enterprises and institutions. The method is simple to operate, good in practical operation effect, capable of saving the cost of manpower and material resources and accurate in summarization and analysis result.

The purpose of the invention is to disclose a biochemical calibration method. The method comprises the following steps: 1, selecting a corresponding calibration substance; 2, preserving and re-melting the calibration substance in step 1 according to a calibration substance preserving technology and a calibration substance re-melting technology; 3, placing the calibration substance in step 2 in a sample holder in a calibrating instrument; 4, checking the calibrating result of the calibrating instrument, and calibrating a recorded and preserved original result; and 5, determining a constant value quality control substance or a routinely used quality control substance, and judging whether the obtained determination result is in an allowed range or not. The calibration substance in the step 2 comprises a calibration substance suitable for a Roche C501 fully-automatic biochemical analyzer. The biochemical calibration method has the advantages of simplicity in operation, scientific flow, clear standards and good practical operation effect, and solves the technical problems of high cost, unscientific flow, unclear standards, poor practical operation effect, high operation difficulty and difficulty in duplication of biochemical calibration methods.

The invention aims at disclosing a biochemical luminescence analysis and calibration method. The method is characterized by comprising the following steps: (1) selecting a corresponding calibration product; (2) operating the calibration product in the step (1) according to a storage method and a re-melting method of the calibration product; (3) putting the calibration product in the step (2) into a sample frame of a calibration instrument; (4) examining a calibration result of the calibration instrument, and correcting record retention of an original result; and (5) measuring a fixed value quality control product or a conventionally used quality control product, and determining whether results are in an allowed range, wherein the calibration product in the step (2) comprises a calibration product applicable to a Roche's E601 electrochemical luminescence analysis meter. The biochemical luminescence analysis and calibration method has the advantages that the operation is simple, the flow is scientific, the standard is defined, the practical operation effect is good, and the technical problems of a biochemical analysis and calibration method that the cost is high, the flow in unscientific, the standard is undefined, the practical operation effect is poor, and the copy operation is difficult are solved.

The invention discloses a method for quantitative detection of EB virus DNA and relates to the technical field of biological detection. The method for quantitative detection of EB virus DNA comprisesthe steps that a nucleic acid releasing agent is adopted for rapid splitting decomposition, EBV-DNA in a sample is released, different extracting modes are adopted for plasma samples, peripheral bloodsamples, pharyngeal swab samples and negative positive controls and are assisted by components such as PCR reaction liquid, a real-time fluorescent quantitative PCR detection technology is applied ona fluorescent quantitative PCR instrument, and quantitative detection of EB virus DNA is achieved through change of fluorescence signals. An EB virus can be determined qualitatively and quantitatively according to accurate reported values of EBV DNA. The method has the advantages of being rapid, accurate and convenient to operate and the like.

The invention discloses a DNA library construction method for fetus chromosome aneuploid detection. The method comprises the steps of 1, performing physical interrupting on a sample DNA, and obtaininga fragmented DNA solution; 2, performing tail end repairing and magnetic bead purification on the fragmented DNA, and obtaining the purified blunt end DNA; 3, performing joint connection on the bluntend DNA, and obtaining DNA of the joint; 4, performing PCR amplification on the DNA of the joint, and obtaining a library, wherein according to step 3, the blunt end DNA, nuclear-free water, connection buffer solution, dNTP, DNA ligase, P1 joint and label sequences X are sequentially added into the centrifugal tube and mixed to be uniform, and centrifuging and purifying are performed after the reaction; operation is easy, the flow path is scientific, the standard is clear, and the practical operation effect is good.

The invention discloses a data routing platform management system for a financial enterprise. The data routing platform management system comprises a data source management platform; wherein the data source management platform is provided with a data management module, a calling party module, a system service module, an interface supplier module, a monitoring analysis module and an authority control module; the calling party module opens one or more services through the data management module, the data management module provides a unified calling form for the calling party module, when the calling party module initiates service calling, a specific service API is called through the system service module, and the calling party module initiates service calling; and a specific implementation interface is switched through the interface supplier module, and the interface supplier module provides a specific interface. Through the unified management of the data of the data source management platform, the data exchange process and standard in the management system are clarified, and the effective sharing of various data and the continuous monitoring, analysis, feedback and correction of the data quality are realized.

The invention relates to a method for testing and evaluating the energy consumption performance of a steel-composite material anti-collision facility structure. Belongs to the field of road and bridge anti-collision facility testing. According to the step, the steel-composite material anti-collision facility is impacted through the pendulum bob device, and the instantaneous movement speed before the pendulum bob device impacts the steel-composite material anti-collision facility and the instantaneous movement speed after the pendulum bob device impacts the steel-composite material anti-collision facility and is separated are obtained through a speed sensor; the kinetic energy reduction value of the pendulum bob device is obtained through calculation, and the steel-composite material anti-collision facility is evaluated through the kinetic energy reduction value. According to the method, an anti-collision facility energy consumption capacity calculation method based on the instantaneous speed before and after impact and the kinetic energy theorem is provided, and a clear evaluation standard grade is formulated. The evaluation steps are clear and easy to implement, the comparison result is visual, the characteristics of the energy consumption performance of the structure can be fully reflected, the practical requirements of engineering are met, and good guiding significance is achieved for production and development of steel-composite material anti-collision facilities.

The invention relates to a shallow-buried bias tunnel operation period surrounding rock and lining structure overall stability evaluation method, and belongs to the technical field of tunnel monitoring. Aiming at the operation safety problem a tunnel, a shallow-buried bias tunnel operation period overall stability analysis and evaluation method based on numerical limit analysis is provided, and the method comprises the steps of: obtaining a surrounding rock mass damage judgment standard (limit strain value) through equivalent rock numerical test analysis; then obtaining a damage judgment standard (limit strain value) of the concrete material through literature research and theoretical analysis; and finally, establishing a tunnel simulation analysis model. Under the condition that lining force structural parameters are kept unchanged, the lining force is reduced, the strength parameters of the surrounding rock are continuously reduced, the stable state and instability failure mode of the surrounding rock are analyzed through the limit strain of the surrounding rock, the structure safety state, vulnerable key parts, damage characteristics and instability failure mechanism are analyzed through the limit strain distribution rule of lining concrete, and then an overall stability evaluation method system is formed.

The invention discloses a library construction method for DNA. The method comprises the steps that form processing, sample preparation, reagent and consumable material preparation and instrument preparation are conducted; tail end repairing mix is added, and incubation of an incubator is subjected to tail end repairing; B1 magnetic beads are added for carrying out first-step purification, washingis carried out two times by using 75% of ethyl alcohol, and TE is dissolved to obtain a purified flat-end DNA sample; the sample is transferred to a subpackaged PCR board of a corresponding label, blowing and beating and uniform mixing are carried out, and joint connection is performed; B2 magnetic beads are added for carrying out purification, and 75% of ethyl alcohol is prepared when the sampleis put on a shelf; washing is carried out two times by using the 75% of ethyl alcohol, TE is dissolved and is transferred to the subpackaged PCR board, and blowing and beating and uniform mixing are carried out to conduct PCR; B2 magnetic beads are added for carrying out purification, when the sample is put on the shelf for 8 minutes, 75% of ethyl alcohol is prepared, washing is carried out two times by using the ethyl alcohol, and 50 TE is dissolved; a scientific and reasonable process makes connection activity be strengthened and improves the joint connecting efficiency so that more repairedDNA segments are successfully connected to a joint sequence, amplification is carried out by using a primer complemented with the joint sequence, and a sequencing library with more target amplification segments is obtained.

The invention aims to discloses a biochemical luminescence water quality test method which is characterized by comprising the following steps: step 1, while a sample is detected or before water is made by using a water purification machine, detecting the electrical conductivity of the water and replacing a filter element and/or a reverse osmosis membrane of a water purifying device when the electrical conductivity exceeds an allowable range; step 2, collecting 20 ml of water for reagent from water in all water purification equipment by using a sterile container, performing microbial assessment and replacing the filter element of the water purifying device if microorganisms in the water exceed the standards. The allowable range comprises the water for the reagent, water for an instrument and ordinary water, and the maximum electrical conductivities are 0.1 microsecond/cm, 1.0 microsecond/cm and 10 microseconds/cm respectively; the microbial standard comprises the water for the reagent and the water for the instrument, and the maximum microbial contents are 10 and 1000 respectively. The biochemical luminescence water quality test method is simple, convenient and flexible to operate, has the advantage of simple operation of a conventional dyeing method and is scientific in process, clear in standard and good in practical operation effect.