Method for judging whether spirulina strains can be applied in large-scale aquaculture production

A spirulina, large-scale technology, applied in judging whether spirulina strains can be used in the field of large-scale aquaculture production, can solve problems such as high cost, cumbersome procedures, large workload, etc., and achieve the effects of low cost and clear standards

Inactive Publication Date: 2013-01-02
ZHEJIANG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Although this method is practical, the procedure is cumbersome, the workload is heavy, the cycle is long, and the cost is high

Method used

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  • Method for judging whether spirulina strains can be applied in large-scale aquaculture production
  • Method for judging whether spirulina strains can be applied in large-scale aquaculture production
  • Method for judging whether spirulina strains can be applied in large-scale aquaculture production

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Embodiment Construction

[0021] Detailed technical scheme of the present invention can be implemented through the following steps:

[0022] 1. Selected materials: the 5 strains of Spirulina platensis strains used to construct the identification standard are known Sp-4, Sp-12, Sp-17, Sp-18 and Sp-37 (Zhejiang University Institute of Nuclear Agricultural Sciences Biological Resources and Molecular Engineering Laboratories are also preserved), and performed well in the laboratory culture experiments that can be automatically regulated by the environment such as temperature and light. Among them, Sp-4, Sp-12 and Sp-17 have strong adaptability to the environment, and they can be used in large-scale It is widely used in production and breeding; while Sp-18 and Sp-37 cannot be used as good production varieties due to their poor adaptability.

[0023] 2. Reagents and instruments selected: Taq DNA polymerase and agarose are products of Shanghai Sangon Bioengineering Company; the cloning vector pMD18-T, dNTP, D...

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Abstract

The invention belongs to a technology for development and application of spirulina, and aims at providing a method for judging whether spirulina strains can be applied in large-scale aquaculture production. The method comprises the following steps of: carrying out sequencing on ftsZ genes of the identified spirulina strains, and carrying out variance analysis on site numbers and phylogenetic treeconstruction on the basis of a ftsZ gene sequence together with the known spirulina platensis; and if the identified spirulina strains and the ftsZ gene sequence of the known spirulina platensis strains are gathered into one type, showing that the production traits of the identified spirulina strains are good, and the identified spirulina strains can be applied in large-scale aquaculture production. Compared with the conventional method, the new method for identifying the good and bad production traits of the spirulina strains by applying the ftsZ gene sequence not only is simple, fast, standard and accurate, but also is low in cost, and is suitable for large-scale and high-throughput screening.

Description

technical field [0001] The invention belongs to the technology for the development and application of spirulina, in particular to a method for judging whether a spirulina strain can be applied to large-scale cultivation and production. Background technique [0002] Spirulina is a photosynthetic and oxygen-releasing prokaryotic filamentous microalgae in a regular spiral shape, a genus of Cyanophyta, Oscillatoriales, and Oscillatoriaceae [Wuhan Plant Scientific Research, 1997, 15(4): 369-374], because it is rich in high-quality protein and a variety of biologically active substances, it has received great attention at home and abroad, and has formed a huge Spirulina on the basis of a large number of studies. Industry, and used in food, biomedicine and health care, feed, fine chemicals and other fields. Spirulina platensis in this genus is the most widely used species in commercial breeding production and development at home and abroad. It is worth pointing out that numerous ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/89
Inventor 汪志平董丹丹邵斌蓝瑾瑾王景梅刘新颖于金鑫吕蓓芬陈子元
Owner ZHEJIANG UNIV
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