42results about How to "Suitable for high-throughput screening" patented technology

Provided are a cell model and screening method for screening inhibitors of the tubercle bacillus. Provided is a cell which expresses the tubercle bacillus proteins L12 and L10, wherein the L12 encoding gene and the transcription activation domain of its transcription factor are located in one expression vector, and the L10 encoding gene and the DNA binding domain of its transcription factor are located in another expression vector. The present invention also relates to the use of the compound shown in formulae I or II in preparing a blocker of the interaction between proteins L12 and L10 of tubercle bacillus, an anti-tubercle bacillus drug, or a drug for treating and / or preventing tuberculosis.

The invention discloses a method for preparation of a liver fibrosis animal model with juvenile zebrafish. The method adopts juvenile zebrafish as the model animal, and selects diethylnitrosamine andethanol as the modeling drug. The zebrafish genome adopted by the method provided by the invention is similar to the human genome, and presents physiological characteristics very similar to human, andcan be well applied to study of various human diseases. Also the method has the advantages of short modeling cycle, low cost and simple operation. The obtained model can be applied to high throughputscreening of various drugs.

The invention relates to a spirulina production technology, and aims at providing a method for identifying advantages and disadvantages of the spirulina filament drainage performance through RAPD detection. The method comprises the steps that spirulina platensis liquid is taken from a cultivating pool, an algal filament monomer with the length larger than or equal to 300 micrometer is picked through a capillary pipette micro-separation method, and an algal filament colony is cultivated; sampling is conducted on the obtained algal filament colony, and then random amplification polymorphic DNA analysis and detection are conducted; if a final 590 bp band cannot be amplified through a primer S90, it is indicated that the identified algal filaments have the good drainage performance; otherwise, it is indicated that the identified algal filaments is poor in drainage performance. According to the method, the advantages and disadvantages of the spirulina filament drainage performance are identified on the basis of a RAPA technology, compared with a conventional method, the method is simple, efficient, low in cost and suitable for high-throughput screening.

The invention provides a method for detecting novel bacillus cereus. The detection method comprises the following steps: 1) extracting a deoxyribonucleic acid (DNA) in a to-be-detected sample; and 2) by taking the DNA obtained in the step 1) as a template, carrying out real-time fluorescent quantization polymerase chain reaction (PCR) detection, wherein the target for real-time fluorescent quantization PCR detection is a bacillus cereus murB gene. The invention also provides a kit for detecting bacillus cereus. The kit comprises a primer for amplification of the bacillus cereus murB gene.

The invention provides a cancer combined diagnosis marker comprising lncRNA H19 and lncRNA PTCSC3, and application of the cancer combined diagnosis marker in preparation of a reagent for cancer diagnosis, cancer prognosis evaluation or cancer treatment monitoring. The lncRNA H19 and the lncRNA PTCSC3, serving as the cancer combined diagnosis marker, have the advantages of high sensitivity, high specificity and accurate detection result, and can be applied to classification of cancer samples. The invention also provides a primer, a detection probe and a detection chip for cancer diagnosis, and a kit comprising the same. The expression contents of the lncRNA H19 and the lncRNA PTCSC3 in the samples are detected by using the primer, the detection probe, the detection chip or the kit respectively, lymph node metastasis of thyroid cancer can be effectively judged, and reliable information is provided for diagnosis, prognosis evaluation and curative effect monitoring of the thyroid cancer.

The invention relates to a spirulina production technology, and aims to provide a method for identifying the draining performance of spirulina filaments by utilizing a scanning electron microscope. The method comprises the following steps: extracting spirulina liquid of spirulina platensis from a cultivation pool, selecting spirulina filament monomers with the lengths larger than or equal to 300 [mu]m by using a capillary pipet microseparation method, and cultivating the spirulina filament monomers into a spirulina filament group; sampling the obtained spirulina filament group, and observing the sampled spirulina filament group by using the scanning electron microscope; if no milky white adhesive substance exists between the spirulina filaments, indicating that the identified spirulina filaments have good draining performance; and if the milky white adhesive substance exists between the spirulina filaments, indicating that the draining performance of the identified spirulina filaments is poor. Compared with a traditional method, the method for identifying the draining performance of the spirulina filaments based on the scanning electron microscope technology is simple and convenient, efficient and low in cost, and is suitable for high-throughput screening.