42results about How to "Suitable for high-throughput screening" patented technology

The invention provides a cancer combined diagnosis marker comprising lncRNA H19 and lncRNA PTCSC3, and application of the cancer combined diagnosis marker in preparation of a reagent for cancer diagnosis, cancer prognosis evaluation or cancer treatment monitoring. The lncRNA H19 and the lncRNA PTCSC3, serving as the cancer combined diagnosis marker, have the advantages of high sensitivity, high specificity and accurate detection result, and can be applied to classification of cancer samples. The invention also provides a primer, a detection probe and a detection chip for cancer diagnosis, and a kit comprising the same. The expression contents of the lncRNA H19 and the lncRNA PTCSC3 in the samples are detected by using the primer, the detection probe, the detection chip or the kit respectively, lymph node metastasis of thyroid cancer can be effectively judged, and reliable information is provided for diagnosis, prognosis evaluation and curative effect monitoring of the thyroid cancer.

The invention provides a high-performance liquid detection and content determination method for ectoine. Chromatographic conditions are as follows: the chromatographic column adopts an adamantine-bonded chromatographic column; the detector adopts a DAD detector; the mobile phase comprises a mobile phase A and a mobile phase B; the mobile phase A is methanol; the mobile phase B is an aqueous buffersolution of monopotassium phosphate; and the elution mode adopts isocratic elution. By utilizing the chromatographic conditions, a test solution and a standard solution are injected into a liquid chromatograph, the chromatogram is recorded, the peak area is calculated according to an external standard method, and the content of ectoine in the sample can be accurately determined. The method disclosed by the invention is short in detection time, excellent in separation effect, high in precision and accuracy and low in cost and has an important application value in aspects of strain screening and related quantitative researches during ectoine production.

The invention discloses a quick detection method by using an interior label and taking a sigD/sigE gene of salmonella as a target through reverse transcription fluorescent quantitation PCR (Polymerase Chain Reaction). In the quick detection method, an RNA (Ribonucleic Acid) interior label obtained by PCR amplification for three times is composed. In addition, the invention further provides a corresponding kit and application. The method disclosed by the invention can be widely applied to the fields such as research of laboratories, food safety and health and hygiene. The phenomenon of false negative missed detection can be avoided, so that the PCR amplification is effectively monitored; in addition, the detection specificity of salmonella is strong, so that dead bacteria and live bacteriacan be effectively distinguished; the quickness and high detection sensitivity are obtained; and the method can be applied to high-throughput screening of the salmonella in the food.

The invention relates to a rapid detection method for RSK4 enzyme activity and application thereof. The detection method comprises the follows steps: (1) taking a sample to be tested, a blank controland a positive control, and respectively co-incubating with RSK4 enzyme and an RSK polypeptide substrate/ATP mixed liquor to carry out a kinase reaction, thus producing ADP; (2) respectively adding anADP-Glo<TM> reaction reagent into three groups of kinase reaction systems in step (1) to co-incubate, terminating the kinase reaction and exhausting the remaining ATP; (3) respectively adding a kinase detection reagent to the three reaction systems of step (2), co-incubating to convert ADP into ATP, and reading a luminescence value RLU of newly synthesized ATP; (4) calculating the enzyme activityaccording to the following formula: enzyme activity=(RLU(Sample)-RLU(Blank))/(RLU(Pos.Ctrl)-RLU(Blank)) is multiplied by 100%. The method is based on an ADP-Glo<TM> kinase assay to directly measure the ATP consumed in the reaction to quantify the progress of the reaction. The method is operated under ordinary laboratory conditions to avoid radiation injury to operators and the environment; the system is stable, measured data has high accuracy, and the method can be applied to screening of RSK4 target inhibitors and screening of RSK4 target anti-tumor drugs.

The present invention provides a new Bacillus cereus detection method, which comprises: 1) extracting DNA from a sample requiring detection; and 2) adopting the DNA obtained from the step 1) as a template to carry out real-time fluorescence quantitative PCR detection, wherein a target detected by the real-time fluorescence quantitative PCR detection is Bacillus cereus murB gene. The present invention further provides a Bacillus cereus detection kit, which comprises primers for Bacillus cereus murB gene amplification.

The invention discloses a preparation method and uses of a nanometer artificial antibody targeting brain natriuretic peptide. According to the present invention, a plurality of functional monomers anda cross-linking agent are polymerized to form a gel nanoparticle artificial antibody, wherein the affinity and the selectivity of the artificial antibody to BNP are regulated by changing the ratio ofthe functional monomers; by combining with a molecular imprinting technology, the specificity and the selectivity of the nanometer artificial antibody to BNP are further improved by using the full polypeptide or partial polypeptide fragments of the BNP as a template; after screening, the affinity constant KD value of the nanometer artificial antibody to BNP reaches 1.41*10<-11> M, and is equivalent to the affinity constant KD value of antibodies, and the nanometer artificial antibody has good selectivity; the obtained nanometer artificial antibody can achieve the high-selectivity enrichment of low-abundance BNP in serum samples by combing with magnetic nanometer particles, monolithic columns or micro-fluidic chips, and can achieve high-sensitivity detection of BNP through high-sensitivityRaman spectroscopy, fluorescence quantitative detection, ELISA kits, chemiluminescence kits, test paper strips and other methods.

The invention discloses a high-performance liquid phase detection method of carbamazepine. The high-performance liquid phase detection method of carbamazepine adopts a reversed phase C18 chromatographic column and a DAD detector; a mobile phase A is a mixed solution of formic acid-ammonium acetate aqueous solution and acetonitrile; a mobile phase B is a mixed solution of formic acid-ammonium acetate in acetonitrile solution and water; and gradient elution is used. Using the method disclosed by the invention to inject a sample 3 to 5 [Mu]l, the carbamazepine and related substances can be effectively detected; the resolution R can reach 1.5 or more; and the base line of HPLC spectrum is stable and does not drift. The detection time of the high-performance liquid phase detection method of carbamazepine is short, and the high-performance liquid detection process can be completed in only 3 minutes; the high-performance liquid phase detection method of carbamazepine greatly reduces the sample consumption, improves the detection efficiency, and is suitable for high-throughput screening, which can save solvent and reduce cost; and the method disclosed by the invention is used for the determination of the carbamazepine content, which has good linear relationship and high reproducibility, and has significances in the research of raw material medicine, preparation quality and pharmacokinetic quantitative research.

The invention discloses a liquid chromatography-mass spectrometry detection method for glucocorticoid in biological fluid; the method comprises the following steps: (1), carrying out protein precipitation and solid-liquid extraction plate pretreatment on a sample to obtain a test solution and a reference solution; and (2), performing liquid chromatography-mass spectrometry combined detection: using a reversed-phase C12 chromatographic column as a chromatographic column, the mobile phase A being composed of an organic acid salt aqueous solution and acetonitrile in a ratio of 9: 1, and the mobile phase B being composed of an organic acid salt aqueous solution and acetonitrile in a ratio of 1: 9; and performing gradient elution. The method provided by the invention can effectively detect glucocorticoid in human urine and serum, and has the characteristics of good separation effect, stable baseline, short detection time, good linearity, high accuracy, good reproducibility, high sensitivityand high precision.

This invention relates to a model for in vitro high throughput screening of human high-density lipoprotein receptor CLA-1 activity regulators. This invention also relates to a method for utilizing the model to screen CLA-1 activity regulators. Besides, CLA-1 activity regulators, especially CLA-1 stimulator and CLA-1 antagonist screened by this method are also disclosed.