A method for identifying the drainage performance of spirulina algae filaments using scanning electron microscopy

An electron microscope, spirulina technology, applied in biochemical equipment and methods, determination/inspection of microorganisms, material analysis using wave/particle radiation, etc. low cost effect

Active Publication Date: 2020-09-15
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Such a high drying energy consumption not only greatly limits the large-scale application of spirulina powder as a high-quality feed protein source in areas with greater demand such as livestock and poultry and aquaculture, but also some production bases burn coal or even burn coal without authorization to reduce drying costs. firewood, seriously destroying the ecology and polluting the environment
For this reason, for more than 20 years at home and abroad, attempts have been made to reduce the water holding capacity of spirulina algae mud by using dehydration processes such as continuous centrifugation, decompression suction filtration, and adsorption exchange. Due to low force performance and other reasons, none of them worked
At the same time, "random isolation of algae filaments→cultivation into algae filament groups→indoor transfer culture→outdoor expansion cultivation→drainage performance test→use for production and cultivation" is still used at home and abroad. The traditional method is cumbersome, time-consuming, and has a low success rate, and has little effect in actual production applications.

Method used

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  • A method for identifying the drainage performance of spirulina algae filaments using scanning electron microscopy
  • A method for identifying the drainage performance of spirulina algae filaments using scanning electron microscopy

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Embodiment Construction

[0021] The technical solutions of the present invention will be described in detail below in conjunction with specific implementation examples.

[0022] 1. Selection of sample materials: 2 well-known Spirulina platensis strains Sp-3 and Sp-15 that are widely used in large-scale production and cultivation are also preserved in the Institute of Nuclear Agricultural Sciences of Zhejiang University affiliated to the applicant. The applicant guarantees that samples can be provided to the public as needed during the validity period of the patent.

[0023] 2. Reagents and instruments: The filter paper used for filtration is produced by Hangzhou Wohua Filter Paper Co., Ltd.; the chemical reagents are all domestic analytical grades; the BX-53 optical microscope with DP-73 from Japan Olympus Company is used to observe and measure the morphology of algal filaments; the scanning electron microscope AMRAY / KYKY-1000B scanning electron microscope produced by Beijing Analytical Instrument Fac...

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Abstract

The invention relates to a spirulina production technology, and aims to provide a method for identifying the draining performance of spirulina filaments by utilizing a scanning electron microscope. The method comprises the following steps: extracting spirulina liquid of spirulina platensis from a cultivation pool, selecting spirulina filament monomers with the lengths larger than or equal to 300 [mu]m by using a capillary pipet microseparation method, and cultivating the spirulina filament monomers into a spirulina filament group; sampling the obtained spirulina filament group, and observing the sampled spirulina filament group by using the scanning electron microscope; if no milky white adhesive substance exists between the spirulina filaments, indicating that the identified spirulina filaments have good draining performance; and if the milky white adhesive substance exists between the spirulina filaments, indicating that the draining performance of the identified spirulina filaments is poor. Compared with a traditional method, the method for identifying the draining performance of the spirulina filaments based on the scanning electron microscope technology is simple and convenient, efficient and low in cost, and is suitable for high-throughput screening.

Description

technical field [0001] The invention belongs to the production technology of spirulina, and in particular relates to a method for identifying the drainage performance of spirulina algal filaments by using a scanning electron microscope. Background technique [0002] Spirulina is a kind of prokaryotic filamentous microalgae with photosynthetic oxygen release and regular spiral, which is a genus of Cyanophyta, Oscillatoriales and Oscillatoriaceae [Wuhan Botany Research, 1997, 15(4):369-374]. Because it is rich in high-quality protein and a variety of biologically active substances, it has attracted great attention at home and abroad, and has formed a huge Spirulina industry on the basis of a large number of researches. It has become the world's largest research and development scale and the most extensive application prospects. microalgae. At present, the production of spirulina powder at home and abroad is generally cultivated in a circular racetrack pool, and then the alga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N23/2251G01N23/2202C12Q1/02
CPCC12Q1/02G01N23/2202G01N23/2251G01N2223/07G01N2223/102G01N2333/195
Inventor 汪志平汪凡越李晨晨
Owner ZHEJIANG UNIV
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