Quick detection method of TGFbetaR1(T204D) enzyme activity, and application thereof
A detection method and enzyme activity technology, applied in the field of biochemistry, can solve problems such as abnormal protein phosphorylation, and achieve the effects of simple steps, shortened detection time, and low sample consumption.
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[0036] Example 1: Rapid detection method of TGFβR1 (T204D) enzyme activity
[0037] In this example, TGFβR1 (T204D) recombinant protein, TGFβR1 substrate, 5X polypeptide buffer and DTT were all purchased from Signalchem Company, ADP-Glo TM Kinase reagents were purchased from Promega, and Staurosprine was purchased from MCE.
[0038] 1. A rapid detection method for TGFβR1 (T204D) enzyme activity, comprising the following steps:
[0039] (1) Take the sample to be tested, the blank control substance, and the positive control substance and incubate with the TGFβR1 (T204D) enzyme reagent and the TGFβR1 substrate / ATP mixture respectively to perform the kinase reaction to generate ADP; the sample to be tested, the positive control substance, and the TGFβR1 (T204D) ) Enzyme reagent, TGFβR1 substrate / ATP mixture all use buffer as solvent, wherein the positive control substance is a solution formed by dissolving staurosporine, a universal kinase inhibitor, in buffer, with a concentrati...
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