The invention discloses a purification method for blackberry
anthocyanin. The purification method includes the steps of resin pretreatment and blackberry
anthocyanin crude extract purification. Efficient purification of the blackberry
anthocyanin can be achieved, and the purity of the obtained blackberry anthocyanin is high. According to the purification method,
polyamide resin is selected as adsorbent and has the advantages that the large specific area and the large
granularity are achieved, adsorption and
desorption capacity is high, cost is low, regeneration is easy, and a preparation method is simple. Through research on the static and dynamic adsorption and
desorption process of the blackberry anthocyanin, purification conditions are optimized, the initial concentration of the blackberry anthocyanin, the pH value of adsorption liquid, the pH value of eluent and the optimal value of
ethanol concentration are determined, and the adsorption rate and the
elution rate of the blackberry anthocyanin are both high. According to the method, time-saving and efficient purification of the blackberry anthocyanin can be achieved, purification time can be shortened, and cost can be reduced.