37results about How to "Cheap purification" patented technology

The invention discloses four sets of oligonucleotide sequences for recognition and identification of vibrio anguillarum, and a screening method of the oligonucleotide sequences, and relates to the technical field of recognition detection of vibrio anguillarum. Two ends of each oligonucleotide sequence are fixed sequences, intermediate sequences are arrayed according to the order of 5'-3' as follows: a first set contains two sequences of H1 and H2, a second set contains five sequences of H5, H6, H26, H38 and H33, a third set contains three sequences of H12, H25 and H42, and a fourth set contains a sequence of H28; and any of the oligonucleotide sequences in each set serves as the intermediate sequence of an aptamer to be used for vibrio anguillarum detection. The screening method of the sequences sequentially comprises the steps of random oligonucleotide library synthesis, combination, separation, PCR amplification and high-throughput sequencing which are circulated for 3-5 rounds, andfinally, the target sequences are obtained. The sequence length of the aptamer of the vibrio anguillarum is shortened, and the screening method is quick.

The invention discloses an organic and inorganic hybrid bismuth and iodine anionic cluster-based semiconductor material and a preparation method thereof. The molecular structural formula of the organic and inorganic hybrid semiconductor material is (MV)2(Bi4I16), MV in the formula is organic cationic methyl viologen with two unit positive charges, and the (Bi4I16) anion in the material is a four-nuclear cluster structural anion composed of trivalent bismuth ions and iodide ions. Through a coordination reaction between the bismuth iodide and a solution of methyl viologen iodide, the organic and inorganic hybrid semiconductor material which is good in semiconductor performance and thermal stability is conveniently prepared with low price, the organic and inorganic hybrid semiconductor material is moderate in energy gap and good in thermal stability and can be applied to the technical field of optoelectronic materials.

The invention relates to a preparation method of esomeprazole magnesium. The method comprises the steps that: esomeprazole is dissolved in an organic solvent; a potassium-containing alkali or salt is subjected to a reaction with esomeprazole, such that esomeprazole potassium is produced; and esomeprazole potassium is subjected to a displacement reaction with a magnesium salt, such that esomeprazole magnesium is prepared. The invention also relates to a refining method of esomeprazole magnesium. With the method provided by the invention, the purity of the prepared esomeprazole magnesium is higher than 99.9%, the yield is higher than 82%, and esomeprazole magnesium has good morphological stability. With the method, requirements by factory pharmacy for purity and yield can be satisfied.

The present invention discloses (pichia pastoris) CGMCC No1622, which can produce metal protease and a manufacturing method of obtaining the bacterial strain. Through designing a DNA primer, 1005 basic group is obtained by using PCR method and increasing from the total DNA of the Bacillus licheniformis XJT9503, and then cloned on the Pmd18-T carrier, after making a sequence analysis and measure to the obtained gene, the high temperature neutral protease gene is EMP, the snatch size is 942 basic group which expresses 314 aminophenol, and a Bacillus subtilis system is made use of validating and affirming the obtained gene. The present invention provides an effective way for making the extensive ferment and production and improving the output of enzyme.

The invention provides a matrix dispersion purification tube for phthalate ester detection, and relates to development of a pretreatment purification tube for detecting various phthalate esters in different matrixes. A purification process of the tube comprises: (1) based on different matrixes, placing a filler in an empty purification tube according to a ratio regulated by a requirement; (2) selecting appropriate extraction solvents to extracted various phthalate esters according to the different matrixes; (3) pouring the extracted phthalate ester solutions into an extraction tube; and (4) after completely mixing, purifying the filler, removing interferents, centrifugating for 5 min at a speed of 4000 r/min, taking the supernatant, and analyzing. With the matrix dispersion purification tube, the phthalate esters in various matrixes can be rapidly and cheaply purified, characteristics of economy, environmental protection and stability are provided, GPC instrument purification is not required, and analysis accuracy and no damage on the instrument are ensured.

The present invention is directed to methods of preparing linear polymers such as polyalkylene oxides containing a terminal amine in high purity. One preferred method includes reacting a polyalkylene oxide such as polyethylene glycol containing a terminal azide with a phosphine-based reducing agent such as triphenylphosphine or an alkali metal borohydride reducing agent such as sodium borohydride in a solvent to reflux. The resultant polymer-amines are of sufficient purity so that expensive and time consuming purification steps required for pharmaceutical grade polymers are avoided.

The present invention is directed to methods of preparing linear polymers such as polyalkylene oxides containing a terminal amine in high purity. One preferred method includes reacting a polyalkylene oxide such as polyethylene glycol containing a terminal tosylate with a protected amine salt to form a polymer containing a terminal protected amine and thereafter deprotecting the polymer containing the terminal protected amine to form the polymer having a terminal amine. The resultant polymer-amines are of sufficient purity so that expensive and time consuming purification steps required for pharmaceutical grade polymers are avoided.

The invention provides a varicella-herpes zoster mRNA vaccine composition, and a preparation method and application thereof. The vaccine composition comprises a messenger ribonucleic acid (mRNA) sequence for coding varicella-herpes zoster virus glycoprotein E, a derivative sequence of the messenger ribonucleic acid (mRNA) sequence and lipid nanoparticles (LNP), and the messenger ribonucleic acid (mRNA) sequence is prepared into particles with the diameter of 20-400 nanometers through microfluidic equipment. The vaccine composition can specifically enhance humoral immune response and cellular immune response against varicella-herpes zoster glycoprotein E, can be used as a varicella vaccine which does not cause latent infection of vaccine strains, and can also be used as a herpes zoster vaccine with unlimited productivity. All the components in the vaccine composition can be widely obtained, so that the vaccine cost is effectively reduced, and the vaccine yield is increased.

The invention provides a method for preparing long-chain fatty acid monobenzyl ester. The method comprises the following steps: 1) carrying out esterification reaction on long-chain fatty diacid to obtain long-aliphatic chain dibenzyl ester; and 2) carrying out hydrolysis reaction on the long-aliphatic chain dibenzyl ester to obtain long-chain fatty diacid monobenzyl ester; wherein the long-chainfatty diacid has a structure shown as formula (A), and X is an integer of 6-32. Compared with the prior art, the method has the advantages of cheap and easily available reaction reagent, simple operation, low product related impurity content, simple intermediate purification, short production period, high yield, low cost, little generated waste liquid, no generation of solid waste and low environmental protection pressure.

The invention provides a cefminox sodium preparation method, which comprises: (1) dispersing a compound having a structure represented by a formula (VII) in an organic solvent A to prepare a solutionor a suspension, adding a compound having a structure represented by a formula (VII), and carrying out vacuum drying to obtain a compound having a structure represented by a formula (VI); (2) dispersing the compound with the structure represented by the formula (VI) into an organic solvent C, adding a compound having a structure represented by a formula (V) in a dropwise manner, and drying to obtain a compound having a structure represented by a formula (IV); (3) dispersing the compound having the structure represented by the formula (IV) in an organic solvent, and removing the solvent by rotary evaporation under a pressure reducing condition to obtain an organic phase containing a compound having a structure represented by a formula (II); and (4) adding purified water into the solution containing the compound having the structure represented by the formula (II), heating, adjusting the pH value, adding a compound having a structure represented by a formula (III), carrying out temperature control stirring, transferring the water phase into a crystallizing tank, crystallizing, and carrying out pressure reducing drying to obtain the cefminox sodium heptahydrate. According to the invention, the synthesis process is shortened, the cost is reduced, and the product yield and the purity are high.

The present invention is directed to methods of preparing linear polymers such as polyalkylene oxides containing a terminal amine in high purity. One preferred method includes reacting a polyalkylene oxide such as polyethylene glycol containing a terminal azide with a phosphine-based reducing agent such as triphenylphosphine or an alkali metal borohydride reducing agent such as sodium borohydride in a solvent to reflux. The resultant polymer-amines are of sufficient purity so that expensive and time consuming purification steps required for pharmaceutical grade polymers are avoided.

The invention discloses a method for separating and purifying fructooligosaccharides, wherein separating and purifying steps include: fermenting cane sugar through Phaffia rhodozyma JMU-MVP14 to obtain a fermentation fluid containing the fructooligosaccharides and astaxanthin; centrifugally separating the fermentation fluid through a centrifuge, extracting the lower-layer astaxanthin, heating the supernatant fluid through a boiling water bath for enzyme inactivation and then purifying the supernatant fluid through a microfiltration membrane, wherein the obtained filtrate flows through a chromatographic column filled with an absorption medium to be absorbed; next, eluting the absorption medium using ethanol solution; and evaporating and concentrating the eluate to obtain the fructooligosaccharides. The method is capable of simultaneously separating and purifying the fructooligosaccharides and the astaxanthin in the same batch of fermentation process; the separation operation is low in cost and simple; the obtained fructooligosaccharide product is high in content and meets the requirements of food sanitation.