Creation method and application of functional rice material for reducing blood sugar

Abstract

The invention discloses a method for creating a new material of hypoglycemic transgenic rice. The method comprises the following steps: introducing an expression cassette of an endosperm specific expression 10 * mGLP-1 gene into a plant, and screening to obtain a transgenic plant of which the endosperm specifically expresses the 10 * mGLP-1 gene; the expression cassette for endosperm specific expression of the 10 * mGLP-1 gene comprises a plant endosperm specific promoter and the 10 * mGLP-1 gene connected to the downstream of the plant endosperm specific promoter. The method can endow the seeds with a health-care function, is suitable for large-scale production of the hypoglycemic oligopeptide, and can be used for cultivating transgenic crops with high hypoglycemic oligopeptide content in the stably inherited seeds.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to a creation method and application of a hypoglycemic functional rice material. Background technique [0002] Diabetes can cause metabolic disorders of multiple systems, with a long course of disease and various complications, which will cause varying degrees of damage to the whole body, especially the eyes, kidneys, heart and blood vessels. More than 90% of diabetic patients belong to type II diabetes. Type II diabetes refers to hyperglycemia caused by insufficient insulin secretion and insulin resistance (body tissue insensitivity to insulin) due to partial damage or apoptosis of pancreatic beta cells. At present, a variety of drugs for the treatment of diabetes have been studied, including conventional traditional drugs and new anti-diabetic drugs that have been clinically used for a long time. Although these drugs have obvious therapeutic effects, they are...

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Problems solved by technology

So far, although rice has been used as a bioreactor to produce mGLP-1, its expression level is low (up to 150ug of mGLP1 protein per gram of rice

References Yasuda H, Hayashi Y, Jomori T, Takaiwa F (2006) The correlation between expression and location of a foreigngene product in rice endosperm. Plant Cell Physiol. 47, 756-763.), can not meet the application requirements, and the transgenic plants Still contains a selectable marker gene, which limits the commercialization of large-scale production of mGLP-1

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