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A cancer combined diagnostic marker and its application

A diagnostic marker and cancer diagnosis technology, applied in the field of tumor molecular biology, can solve the problems of low detection sensitivity, inability to provide effective information for thyroid cancer diagnosis, prognosis assessment or curative effect monitoring, and poor specificity

Active Publication Date: 2020-10-20
JIANGSU INST OF NUCLEAR MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention is to solve the low detection sensitivity and poor specificity of the detection markers of thyroid cancer in the prior art, which cannot provide effective information for the diagnosis, prognosis evaluation or curative effect monitoring of thyroid cancer; thus providing a sensitivity High and specific cancer combined diagnostic markers

Method used

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  • A cancer combined diagnostic marker and its application
  • A cancer combined diagnostic marker and its application
  • A cancer combined diagnostic marker and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This embodiment provides a method for measuring the expression levels of lncRNA H19 and lncRNA PTCSC3 in a sample, which specifically includes the following steps:

[0052] 1. Collection of samples

[0053] The postoperative tissue samples of 41 patients with papillary thyroid cancer were randomly collected in Jiangyuan Hospital, Affiliated Hospital of Jiangsu Institute of Atomic Medicine, and the tumor tissues and tissues more than 1 cm away from the tumor tissues were selected as normal tissues adjacent to the tumor, and lncRNA H19 and lncRNA were detected respectively The expression level of PTCSC3.

[0054] 2. To detect the expression levels of lncRNA H19 and lncRNA PTCSC3 in the tumor tissue of patients with papillary thyroid cancer and normal tissues adjacent to the tumor:

[0055] 1) Extraction of total cellular RNA

[0056] (1) Take 50 mg of tumor tissue and 50 mg of normal tissue adjacent to the tumor, wash them with PBS (pH 7.4), add 1 ml Trizol, transfer to...

Embodiment 2

[0080] This embodiment provides a method for measuring the expression levels of lncRNA H19 and lncRNA PTCSC3 in a sample by using a detection probe, which specifically includes the following steps:

[0081] 1) Construct positive standard plasmid

Embodiment 3

[0089] This embodiment provides a method for jointly detecting lncRNA H19 and lncRNA PTCSC3 in a sample to improve the accuracy of sample classification, which specifically includes the following steps:

[0090] 1. The expression levels of lncRNA H19 and lncRNA PTCSC3, which are closely related to lymph node metastasis of thyroid cancer, were detected by qPCR.

[0091] 2. Statistically process the expressions of lncRNA H19 and lncRNA PTCSC3 in the samples measured in step 1 by using mathematical analysis methods, and obtain grading standards with sample classification significance on this basis. Such a mathematical method is preferably implemented by a computer. In this embodiment, these data are used to draw an ROC curve, so as to classify individual samples.

[0092] The full name of the ROC curve is the receiver operating characteristic curve (receiver operator characteristic curve), also known as the receiver operating characteristic curve, which is mainly used in clinical...

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Abstract

The invention provides a cancer combined diagnosis marker comprising lncRNA H19 and lncRNA PTCSC3, and application of the cancer combined diagnosis marker in preparation of a reagent for cancer diagnosis, cancer prognosis evaluation or cancer treatment monitoring. The lncRNA H19 and the lncRNA PTCSC3, serving as the cancer combined diagnosis marker, have the advantages of high sensitivity, high specificity and accurate detection result, and can be applied to classification of cancer samples. The invention also provides a primer, a detection probe and a detection chip for cancer diagnosis, and a kit comprising the same. The expression contents of the lncRNA H19 and the lncRNA PTCSC3 in the samples are detected by using the primer, the detection probe, the detection chip or the kit respectively, lymph node metastasis of thyroid cancer can be effectively judged, and reliable information is provided for diagnosis, prognosis evaluation and curative effect monitoring of the thyroid cancer.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to a combined cancer diagnostic marker, the use of the cancer combined diagnostic marker, and primers, detection probes, detection chips and kits designed based on the marker. Background technique [0002] Thyroid carcinoma (thyroid carcinoma) is the most common malignant tumor of the thyroid gland, which is a malignant tumor derived from thyroid epithelial cells. Its incidence rate accounts for about 1.3%-1.5% of systemic malignant tumors, ranking first among endocrine system diseases. It is a common and frequently-occurring disease in clinical practice, and it keeps increasing year by year. The vast majority of thyroid cancer originates from follicular epithelial cells, which can be divided into papillary carcinoma (60%), follicular adenocarcinoma (20%), undifferentiated carcinoma (15%), medullary carcinoma (7%) ). Among them, papillary carcinoma has cervical ly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 程献张莉俞惠新包建东徐石宸
Owner JIANGSU INST OF NUCLEAR MEDICINE
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