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Method for preparation of liver fibrosis animal model with juvenile zebrafish

An animal model and technology of liver fibrosis, which is applied to the active ingredients of hydroxyl compounds, medical preparations containing active ingredients, and pharmaceutical formulas, can solve the problems of high cost, complicated operation, and long time consumption, and achieve short modeling cycle, Simple operation and less time-consuming effect

Active Publication Date: 2018-04-20
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Most of the liver fibrosis models use rats or mice as experimental animals, and the modeling cycle is mostly ranging from 4 to 13 weeks, which takes a long time;
[0006] 2. Although the modeling cycle of bile duct ligation is only five days, the mortality rate is high, the cost is high, and there are certain requirements for technology;
[0007] 3. As experimental animals, mice and rats are costly and difficult to raise on a large scale and perform high-throughput drug screening, and the existing modeling methods all require operations such as gavage, intraperitoneal injection, and subcutaneous injection, which are complicated to operate

Method used

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  • Method for preparation of liver fibrosis animal model with juvenile zebrafish
  • Method for preparation of liver fibrosis animal model with juvenile zebrafish
  • Method for preparation of liver fibrosis animal model with juvenile zebrafish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of experimental animals:

[0037] Adult zebrafish of the wild-type AB strain: The adult zebrafish of the wild-type AB strain was provided by the Guangdong Provincial Key Laboratory of Human Disease Zebrafish Model and Drug Screening, Southern Medical University.

[0038] Select 56-hour-old healthy AB strain wild-type zebrafish: According to the Westerfield method, the wild-type AB strain zebrafish adult fish were cultured. The photoperiod was maintained at L:D=14h:10h, and the temperature was maintained at 28.5°C. The male and female adult fish mate naturally, and the fertilized eggs are collected in a plate containing the hatching solution of juvenile fish, and placed in a constant temperature incubator at 28.5°C for 56 hours.

[0039] Preparation of experimental reagents:

[0040] Juvenile fish hatching solution: Add 1 / 10000 volume of methylene blue to the water of the zebrafish standard breeding system.

[0041] 1.5% ethanol solution: 3 mL of 100% etha...

Embodiment 2

[0046] Preparation of experimental animals:

[0047] Adult zebrafish of the wild-type AB strain: The adult zebrafish of the wild-type AB strain was provided by the Guangdong Provincial Key Laboratory of Human Disease Zebrafish Model and Drug Screening, Southern Medical University.

[0048] Select 56-hour-old healthy AB strain wild-type zebrafish: According to the Westerfield method, the wild-type AB strain zebrafish adult fish were cultured. The photoperiod was maintained at L:D=14h:10h, and the temperature was maintained at 28.5°C. The male and female adult fish mate naturally, and the fertilized eggs are collected in a plate containing the hatching solution of juvenile fish, and placed in a constant temperature incubator at 28.5°C for 56 hours.

[0049] Preparation of experimental reagents:

[0050] Juvenile fish hatching solution: Add 1 / 10000 volume of methylene blue to the water of the zebrafish standard breeding system.

[0051] 2% ethanol solution: 4mL 100% ethanol wa...

Embodiment 3

[0056] Preparation of experimental animals:

[0057] Adult zebrafish of the wild-type AB strain: The adult zebrafish of the wild-type AB strain was provided by the Guangdong Provincial Key Laboratory of Human Disease Zebrafish Model and Drug Screening, Southern Medical University.

[0058] Select 56-hour-old healthy AB strain wild-type zebrafish: According to the Westerfield method, the wild-type AB strain zebrafish adult fish were cultured. The photoperiod was maintained at L:D=14h:10h, and the temperature was maintained at 28.5°C. The male and female adult fish mate naturally, and the fertilized eggs are collected in a plate containing the hatching solution of juvenile fish, and placed in a constant temperature incubator at 28.5°C for 56 hours.

[0059] Preparation of experimental reagents:

[0060] Juvenile fish hatching solution: Add 1 / 10000 volume of methylene blue to the water of the zebrafish standard breeding system.

[0061] 1.5% ethanol solution: 3 mL of 100% etha...

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Abstract

The invention discloses a method for preparation of a liver fibrosis animal model with juvenile zebrafish. The method adopts juvenile zebrafish as the model animal, and selects diethylnitrosamine andethanol as the modeling drug. The zebrafish genome adopted by the method provided by the invention is similar to the human genome, and presents physiological characteristics very similar to human, andcan be well applied to study of various human diseases. Also the method has the advantages of short modeling cycle, low cost and simple operation. The obtained model can be applied to high throughputscreening of various drugs.

Description

technical field [0001] The invention relates to the field of animal models, in particular to a method for preparing an animal model of liver fibrosis using zebrafish juveniles. Background technique [0002] Liver fibrosis is a common pathological link in the development of almost all chronic liver diseases and is reversible. In order to further study the pathogenesis of liver fibrosis and screen out effective drugs for the prevention and treatment of liver fibrosis with high content, the selection and production are similar to those of human liver fibrosis. The animal model is of great significance. [0003] At present, the stable established liver fibrosis models include carbon tetrachloride modeling method, ethanol modeling method, immune modeling method and bile duct ligation method, etc., which have different advantages and disadvantages, and all use mice or rats. As experimental animals, the modeling time ranges from 5 days to 13 weeks. [0004] Defect and deficiency ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/131A61K31/045
CPCA61K31/045A61K31/131A61K2300/00
Inventor 吕志平高磊刘强周楚莹赖裕玲
Owner SOUTHERN MEDICAL UNIVERSITY
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