Method for identifying algal filament draining performance of spirulina by using transmission electron microscope

Abstract

The invention relates to the production technology of spirulina, and aims to provide a method for identifying algal filament draining performance of spirulina by using a transmission electron microscope. The method comprises the following steps: extracting an algae solution of Spirulina platensis from a cultivation pool, selecting algal filament monomers with the lengths of greater than 300 microns by using a capillary pipet microscopic separation method, and cultivating the algal filament monomers into algal filament groups; sampling the obtained algal filament groups, and observing by usingthe transmission electron microscope; if polyphosphate particles inside algae cells are scattered and the maximum diameter is not greater than 0.2 micron, the identified algal filaments are of good draining performance; and if the polyphosphate particles inside algae cells are aggregated and the maximum diameter is greater than 0.4 microns, the identified algal filaments are of poor draining performance. According to the method, the algal filament draining performance of spirulina is identified based on the transmission electron microscope, and compared with a traditional method, the method issimple, convenient, efficient and low in cost, and is applicable for high-flux screening.

Description

technical field [0001] The invention belongs to the production technology of spirulina, in particular to a method for identifying the water-draining performance of spirulina filaments by using a transmission electron microscope. Background technique [0002] Spirulina is a kind of prokaryotic filamentous microalgae with photosynthetic oxygen release and regular spiral, which is a genus of Cyanophyta, Oscillatoriales and Oscillatoriaceae [Wuhan Botany Research, 1997, 15(4):369-374]. Because it is rich in high-quality protein and a variety of biologically active substances, it has attracted great attention at home and abroad, and has formed a huge Spirulina industry on the basis of a large number of researches. It has become the world's largest research and development scale and the most extensive application prospects. microalgae. At present, the production of spirulina powder at home and abroad is generally cultivated in a circular racetrack pool, and then the algae body i...

AI Technical Summary

Problems solved by technology

Such a high drying energy consumption not only greatly limits the large-scale application of spirulina powder as a high-quality feed protein source in areas with greater demand such as livestock and poultry and aquaculture, but also some production bases burn coal or even burn coal without authorization to reduce drying costs. firewood, seriously destroying the ecology and polluting the environment

For this reason, for more than 20 years at home and abroad, attempts have been made to reduce the water holding capacity of spirulina algae mud by using dehydration processes such as continuous centrifugation, decompression suction filtration, and adsorption exchange. Due to low force performance and other reasons, none of them worked

At the same time, "random isolation of algae filaments→cultivation into algae filament groups→indoor transfer culture→outdoor expansion cultivation→drainage performance test→use for production and cultivation" is still used at home and abroad. The traditional method is cumbersome, time-consuming, and has a low success rate, and has little effect in actual production applications.

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